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Protein

7-methylguanosine phosphate-specific 5'-nucleotidase

Gene

NT5C3B

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Specifically hydrolyzes 7-methylguanosine monophosphate (m7GMP) to 7-methylguanosine and inorganic phosphate. The specific activity for m7GMP may protect cells against undesired salvage of m7GMP and its incorporation into nucleic acids. Also has weak activity for CMP. UMP and purine nucleotides are poor substrates (By similarity).By similarity

Catalytic activityi

N7-methyl-GMP + H2O = N7-methyl-guanosine + phosphate.By similarity
CMP + H2O = cytidine + phosphate.By similarity
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38NucleophileBy similarity1
Metal bindingi38MagnesiumBy similarity1
Active sitei40Proton donorBy similarity1
Metal bindingi40Magnesium; via carbonyl oxygenBy similarity1
Binding sitei85CMPBy similarity1
Binding sitei85N(7)-methyl-GMPBy similarity1
Binding sitei202SubstrateBy similarity1
Metal bindingi227MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processNucleotide metabolism
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-GGA-429958 mRNA decay by 3' to 5' exoribonuclease

Names & Taxonomyi

Protein namesi
Recommended name:
7-methylguanosine phosphate-specific 5'-nucleotidaseBy similarity (EC:3.1.3.91By similarity)
Short name:
7-methylguanosine nucleotidase
Alternative name(s):
Cytosolic 5'-nucleotidase 3B
Cytosolic 5'-nucleotidase III-like proteinBy similarity (EC:3.1.3.5By similarity)
Short name:
cN-III-like protein
N(7)-methylguanylate 5'-phosphatase
Gene namesi
Name:NT5C3B
Synonyms:NT5C3L
ORF Names:RCJMB04_11c18
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 27

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289511 – 2897-methylguanosine phosphate-specific 5'-nucleotidaseAdd BLAST289

Proteomic databases

PaxDbiQ5ZKF6

Expressioni

Gene expression databases

BgeeiENSGALG00000003651

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi9031.ENSGALP00000005776

Structurei

3D structure databases

ProteinModelPortaliQ5ZKF6
SMRiQ5ZKF6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni153 – 154Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the pyrimidine 5'-nucleotidase family.Curated

Phylogenomic databases

eggNOGiKOG3128 Eukaryota
ENOG410ZQJ8 LUCA
GeneTreeiENSGT00390000012959
HOGENOMiHOG000244931
HOVERGENiHBG059750
InParanoidiQ5ZKF6
KOiK01081
OMAiIISEECR
OrthoDBiEOG091G0BCN
PhylomeDBiQ5ZKF6
TreeFamiTF314663

Family and domain databases

CDDicd07504 HAD_5NT, 1 hit
Gene3Di3.40.50.1000, 2 hits
InterProiView protein in InterPro
IPR036412 HAD-like_sf
IPR023214 HAD_sf
IPR006434 Pyrimidine_nucleotidase_eu
PfamiView protein in Pfam
PF05822 UMPH-1, 1 hit
SFLDiSFLDG01128 C1.4:_5'-Nucleotidase_Like, 1 hit
SUPFAMiSSF56784 SSF56784, 1 hit
TIGRFAMsiTIGR01544 HAD-SF-IE, 1 hit

Sequencei

Sequence statusi: Complete.

Q5ZKF6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVPELQKSTV RIQQPERVMG VIRAIKEQGC SKLQVISDFD MTLSRFGCNG
60 70 80 90 100
RRCPTSHNIL DNSHVISEDG KKKLKDLLHH YYPIEIDPNR TLEEKRPLMV
110 120 130 140 150
EWWTRAHELL SQQKIQKGDI AQIVRESDVM LRDGFNELFD QLHKYSVPMF
160 170 180 190 200
IFSAGVGDVL EEIIRQANVF YPNVNVVSNY MDFDDSGVLK CFKSPLIHTY
210 220 230 240 250
NKNNSVLQGT AYFQQLSTRT SIILLGDSMG DLTMADGVPS VENILKIGFL
260 270 280
NDKVEEQRGR YLDAYDIVLE SDETLDVVNG ILRYILMET
Length:289
Mass (Da):33,109
Last modified:November 23, 2004 - v1
Checksum:i9DF06DC1F6EF099E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720128 mRNA Translation: CAG31787.1
RefSeqiNP_001006334.1, NM_001006334.1
XP_015155089.1, XM_015299603.1
XP_015155090.1, XM_015299604.1
UniGeneiGga.4321

Genome annotation databases

EnsembliENSGALT00000005786; ENSGALP00000005776; ENSGALG00000003651
GeneIDi420034
KEGGigga:420034

Similar proteinsi

Entry informationi

Entry namei5NT3B_CHICK
AccessioniPrimary (citable) accession number: Q5ZKF6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: November 23, 2004
Last modified: February 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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