UniProtKB - Q5ZJW8 (DTL_CHICK)
Protein
Denticleless protein homolog
Gene
DTL
Organism
Gallus gallus (Chicken)
Status
Functioni
Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1), KMT5A and SDE2. CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1) degradation during S phase or following UV irradiation is essential to control replication licensing. KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL) complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis (By similarity). May play a role in the regulation of the circadian clock (By similarity).By similarity
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- ubiquitin-protein transferase activity Source: Ensembl
GO - Biological processi
- cellular response to DNA damage stimulus Source: UniProtKB
- DNA replication Source: UniProtKB-KW
- positive regulation of G2/M transition of mitotic cell cycle Source: Ensembl
- positive regulation of protein catabolic process Source: Ensembl
- protein monoubiquitination Source: UniProtKB
- protein polyubiquitination Source: UniProtKB
- regulation of cell cycle Source: UniProtKB
- response to UV Source: UniProtKB
- rhythmic process Source: UniProtKB-KW
- signal transduction involved in G2 DNA damage checkpoint Source: UniProtKB
- translesion synthesis Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Biological process | Biological rhythms, DNA damage, DNA replication, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-GGA-8951664, Neddylation |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Denticleless protein homolog |
Gene namesi | Name:DTL Synonyms:CDT2 ORF Names:RCJMB04_15a2 |
Organismi | Gallus gallus (Chicken) |
Taxonomic identifieri | 9031 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archelosauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galloanserae › Galliformes › Phasianidae › Phasianinae › Gallus |
Proteomesi |
|
Subcellular locationi
Cytoskeleton
- centrosome By similarity
Nucleus
- Nucleus By similarity
Other locations
- Chromosome By similarity
Cytoskeleton
- centrosome Source: UniProtKB
Cytosol
- cytosol Source: Ensembl
Nucleus
- nucleolus Source: Ensembl
- nucleoplasm Source: Ensembl
- nucleus Source: UniProtKB
Other locations
- chromosome Source: UniProtKB-SubCell
- Cul4A-RING E3 ubiquitin ligase complex Source: UniProtKB
- Cul4B-RING E3 ubiquitin ligase complex Source: UniProtKB
Keywords - Cellular componenti
Chromosome, Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000274869 | 1 – 720 | Denticleless protein homologAdd BLAST | 720 |
Proteomic databases
PaxDbi | Q5ZJW8 |
PRIDEi | Q5ZJW8 |
Expressioni
Gene expression databases
Bgeei | ENSGALG00000009837, Expressed in testis and 5 other tissues |
Interactioni
Subunit structurei
Component of the DCX(DTL) E3 ubiquitin ligase complex, at least composed of CUL4 (CUL4A or CUL4B), DDB1, DTL/CDT2 and RBX1.
By similarityProtein-protein interaction databases
STRINGi | 9031.ENSGALP00000015973 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 43 – 85 | WD 1Add BLAST | 43 | |
Repeati | 92 – 131 | WD 2Add BLAST | 40 | |
Repeati | 134 – 174 | WD 3Add BLAST | 41 | |
Repeati | 211 – 250 | WD 4Add BLAST | 40 | |
Repeati | 266 – 305 | WD 5Add BLAST | 40 | |
Repeati | 310 – 351 | WD 6Add BLAST | 42 | |
Repeati | 355 – 395 | WD 7Add BLAST | 41 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 164 – 167 | DDB1-binding motifBy similarity | 4 | |
Motifi | 193 – 200 | Nuclear localization signalSequence analysis | 8 | |
Motifi | 240 – 243 | DDB1-binding motifBy similarity | 4 |
Sequence similaritiesi
Belongs to the WD repeat cdt2 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0321, Eukaryota |
GeneTreei | ENSGT00530000064210 |
HOGENOMi | CLU_023407_0_0_1 |
InParanoidi | Q5ZJW8 |
OMAi | IWKVSEP |
OrthoDBi | 1288134at2759 |
PhylomeDBi | Q5ZJW8 |
TreeFami | TF324483 |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5ZJW8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MLCRALLLRA AGHRQSSSLP LQHLLDGYRC SREDDHLSYG EIGMPVPPFG
60 70 80 90 100
CSFSAAPNFE HVLAVANEEG FVRLYDTEAQ NTTKLISKEW QAHSNAVFDL
110 120 130 140 150
AWVPGEHRIV TASGDQTAKV WDVRAGELLG ICKGHQCSLK SVAFSRFEKA
160 170 180 190 200
VFCTGGRDGN IMVWDTRCNK KDGFYRQVNQ ISGAHNVVDR QTPSKLRKKR
210 220 230 240 250
QNLRGLAPLV DFQQSVTVVL LQDEHTLISA GAVDGVIKVW DLRKNYAAYR
260 270 280 290 300
QDPVPSKSFF YPGTSTRKLG YSSLVLDSTG ANLFANCTDD SIYMFNMTSL
310 320 330 340 350
KTFPVAVFSG HQNSTFYIKS SISPDDQFLV SGSSDCNAYI WKVSEPSLPP
360 370 380 390 400
RILVGHSQEV TSIAWCPSDF TKIATCSDDN TVRIWRLQHY PEEEKSVSNK
410 420 430 440 450
AKLVGWVTQK KPEEQRGAGR SASPQSTPAK AFSVGSPCAS SPRPAACAPS
460 470 480 490 500
YSGDLPLSTN TPTVSLKTQM ATACTPAKLS GASPRTSPKL VPSSKMSIKH
510 520 530 540 550
WIARTPCSSP EVGKKTPSPR KALAEVTQSL LETSSTPKAQ HSQAEKRAKR
560 570 580 590 600
RLDCSKEDEA GQKCLQDCSC VTELDHVAKK SKLNLCHLAA GQRACDEGSL
610 620 630 640 650
SLADLDNEHE DSTHSPKELS FPGSLVNPSG TQTPPPVLQS PCERDSDVVD
660 670 680 690 700
KENSSPERKN WLSALGEKLR TGKAGSPPSS YTSSAKRQEA AVVTTSPKTA
710 720
VNTSVSMRKI CTYFHRKPQN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ720316 mRNA Translation: CAG31975.1 |
RefSeqi | NP_001026219.1, NM_001031048.1 |
Genome annotation databases
Ensembli | ENSGALT00000015990; ENSGALP00000015973; ENSGALG00000009837 |
GeneIDi | 421373 |
KEGGi | gga:421373 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ720316 mRNA Translation: CAG31975.1 |
RefSeqi | NP_001026219.1, NM_001031048.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 9031.ENSGALP00000015973 |
Proteomic databases
PaxDbi | Q5ZJW8 |
PRIDEi | Q5ZJW8 |
Genome annotation databases
Ensembli | ENSGALT00000015990; ENSGALP00000015973; ENSGALG00000009837 |
GeneIDi | 421373 |
KEGGi | gga:421373 |
Organism-specific databases
CTDi | 51514 |
Phylogenomic databases
eggNOGi | KOG0321, Eukaryota |
GeneTreei | ENSGT00530000064210 |
HOGENOMi | CLU_023407_0_0_1 |
InParanoidi | Q5ZJW8 |
OMAi | IWKVSEP |
OrthoDBi | 1288134at2759 |
PhylomeDBi | Q5ZJW8 |
TreeFami | TF324483 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Reactomei | R-GGA-8951664, Neddylation |
Miscellaneous databases
PROi | PR:Q5ZJW8 |
Gene expression databases
Bgeei | ENSGALG00000009837, Expressed in testis and 5 other tissues |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR020472, G-protein_beta_WD-40_rep IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 4 hits |
PRINTSi | PR00320, GPROTEINBRPT |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 2 hits PS50082, WD_REPEATS_2, 4 hits PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DTL_CHICK | |
Accessioni | Q5ZJW8Primary (citable) accession number: Q5ZJW8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 6, 2007 |
Last sequence update: | November 23, 2004 | |
Last modified: | December 2, 2020 | |
This is version 112 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families