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Entry version 101 (16 Jan 2019)
Sequence version 1 (23 Nov 2004)
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Protein

Replication protein A 70 kDa DNA-binding subunit

Gene

RPA1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at transcript leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Function’ section specifies the position and type of each DNA-binding domain present within the protein.<p><a href='/help/dna_bind' target='_top'>More...</a></p>DNA bindingi194 – 278OBAdd BLAST85

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionDNA-binding
Biological processDNA replication
LigandMetal-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-GGA-110312 Translesion synthesis by REV1
R-GGA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-GGA-110320 Translesion Synthesis by POLH
R-GGA-174437 Removal of the Flap Intermediate from the C-strand
R-GGA-176187 Activation of ATR in response to replication stress
R-GGA-3108214 SUMOylation of DNA damage response and repair proteins
R-GGA-3371453 Regulation of HSF1-mediated heat shock response
R-GGA-351451 Homologous recombination repair of replication-dependent double-strand breaks
R-GGA-351468 Processing of DNA double-strand break ends
R-GGA-353303 Nucleotide Excision Repair
R-GGA-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
R-GGA-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-GGA-5655862 Translesion synthesis by POLK
R-GGA-5656121 Translesion synthesis by POLI
R-GGA-5656169 Termination of translesion DNA synthesis
R-GGA-5685938 HDR through Single Strand Annealing (SSA)
R-GGA-5685942 HDR through Homologous Recombination (HRR)
R-GGA-5693607 Processing of DNA double-strand break ends
R-GGA-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-GGA-5696395 Formation of Incision Complex in GG-NER
R-GGA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-GGA-5696400 Dual Incision in GG-NER
R-GGA-6782135 Dual incision in TC-NER
R-GGA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-GGA-6783310 Fanconi Anemia Pathway
R-GGA-68962 Activation of the pre-replicative complex
R-GGA-69166 Removal of the Flap Intermediate

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Replication protein A 70 kDa DNA-binding subunit
Short name:
RP-A p70
Alternative name(s):
Replication factor A protein 1
Short name:
RF-A protein 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:RPA1
ORF Names:RCJMB04_17l6
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiGallus gallus (Chicken)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9031 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000539 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000972631 – 614Replication protein A 70 kDa DNA-binding subunitAdd BLAST614

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q5ZJJ2

PRoteomics IDEntifications database

More...
PRIDEi
Q5ZJJ2

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSGALG00000003072 Expressed in 11 organ(s), highest expression level in female gonad

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Component of the heterotrimeric canonical replication protein A complex (RPA).By similarity

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
9031.ENSGALP00000004840

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

More...
ProteinModelPortali
Q5ZJJ2

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q5ZJJ2

Database of comparative protein structure models

More...
ModBasei
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG0851 Eukaryota
COG1599 LUCA

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000012403

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000162322

The HOVERGEN Database of Homologous Vertebrate Genes

More...
HOVERGENi
HBG010502

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q5ZJJ2

KEGG Orthology (KO)

More...
KOi
K07466

Identification of Orthologs from Complete Genome Data

More...
OMAi
NIYLMDT

Database of Orthologous Groups

More...
OrthoDBi
1189265at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q5ZJJ2

TreeFam database of animal gene trees

More...
TreeFami
TF105241

Family and domain databases

Conserved Domains Database

More...
CDDi
cd04476 RPA1_DBD_C, 1 hit
cd04477 RPA1N, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
IPR013955 Rep_factor-A_C
IPR007199 Rep_factor-A_N
IPR031657 REPA_OB_2
IPR004591 Rfa1

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF04057 Rep-A_N, 1 hit
PF08646 Rep_fac-A_C, 1 hit
PF16900 REPA_OB_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF50249 SSF50249, 4 hits

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00617 rpa1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q5ZJJ2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVRLSEGAI AAIMQGENVY KPVLQVINTR AIATGNGPPR YRVLMSDGVN
60 70 80 90 100
TLSSFMLATQ LNPLVEEERL SAHCICQVNR FIVNSLKDGR RVVILMDLDV
110 120 130 140 150
LKTADMVGGT VGNPVPYNEG QGQQRSSAPT ANAAPNKPQQ QDGNLSVAGS
160 170 180 190 200
AAPKYHAPSN QFSKASAPSS VKTPGGTQSK VVPIASLNPY QSKWTICARV
210 220 230 240 250
TQKGQIRTWS NSRGEGKLFS IELVDESGEI RATAFNDQAD KFFPLIELNK
260 270 280 290 300
VYYFTKGNLK TANKQYTAVK NDYEITFNNE TSVVPCDDAQ HLPSVQFDFV
310 320 330 340 350
SISDLENTPK DSIVDVIGIC KSYEDVTKIV VKASNREVSK RNVHLMDTSG
360 370 380 390 400
KLVTATLWGN EAEKFDGSRQ PVIAIKGARV SDFGGRSLSV LSSSTVVVNP
410 420 430 440 450
DSPEAFKLRG WFDSEGQLLE CASISDVRGG SASGVNTNWK TLYEAKSERL
460 470 480 490 500
GQGDKADYFS CVGTIVHLRK ENCMYQACPS QDCNKKVIDQ QNGLYRCEKC
510 520 530 540 550
DREFPNFKYR MMLLVTIADS LDYQWVTCFQ ESAEFILGQS ATFLGELKDK
560 570 580 590 600
NEQAFEEVFQ NANFNTYEFK IRVKLETYND ESRIKATALD VKPVNYREYS
610
KRLIASIRRN AQLG
Length:614
Mass (Da):68,001
Last modified:November 23, 2004 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i21F99B6673CD785C
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AJ720442 mRNA Translation: CAG32101.1

NCBI Reference Sequences

More...
RefSeqi
NP_001006221.1, NM_001006221.1
XP_015151236.1, XM_015295750.1
XP_015151237.1, XM_015295751.1

UniGene gene-oriented nucleotide sequence clusters

More...
UniGenei
Gga.4736

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSGALT00000004849; ENSGALP00000004840; ENSGALG00000003072

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
417563

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
gga:417563

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720442 mRNA Translation: CAG32101.1
RefSeqiNP_001006221.1, NM_001006221.1
XP_015151236.1, XM_015295750.1
XP_015151237.1, XM_015295751.1
UniGeneiGga.4736

3D structure databases

ProteinModelPortaliQ5ZJJ2
SMRiQ5ZJJ2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9031.ENSGALP00000004840

Proteomic databases

PaxDbiQ5ZJJ2
PRIDEiQ5ZJJ2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000004849; ENSGALP00000004840; ENSGALG00000003072
GeneIDi417563
KEGGigga:417563

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
6117

Phylogenomic databases

eggNOGiKOG0851 Eukaryota
COG1599 LUCA
GeneTreeiENSGT00390000012403
HOGENOMiHOG000162322
HOVERGENiHBG010502
InParanoidiQ5ZJJ2
KOiK07466
OMAiNIYLMDT
OrthoDBi1189265at2759
PhylomeDBiQ5ZJJ2
TreeFamiTF105241

Enzyme and pathway databases

ReactomeiR-GGA-110312 Translesion synthesis by REV1
R-GGA-110314 Recognition of DNA damage by PCNA-containing replication complex
R-GGA-110320 Translesion Synthesis by POLH
R-GGA-174437 Removal of the Flap Intermediate from the C-strand
R-GGA-176187 Activation of ATR in response to replication stress
R-GGA-3108214 SUMOylation of DNA damage response and repair proteins
R-GGA-3371453 Regulation of HSF1-mediated heat shock response
R-GGA-351451 Homologous recombination repair of replication-dependent double-strand breaks
R-GGA-351468 Processing of DNA double-strand break ends
R-GGA-353303 Nucleotide Excision Repair
R-GGA-5358565 Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha)
R-GGA-5651801 PCNA-Dependent Long Patch Base Excision Repair
R-GGA-5655862 Translesion synthesis by POLK
R-GGA-5656121 Translesion synthesis by POLI
R-GGA-5656169 Termination of translesion DNA synthesis
R-GGA-5685938 HDR through Single Strand Annealing (SSA)
R-GGA-5685942 HDR through Homologous Recombination (HRR)
R-GGA-5693607 Processing of DNA double-strand break ends
R-GGA-5693616 Presynaptic phase of homologous DNA pairing and strand exchange
R-GGA-5696395 Formation of Incision Complex in GG-NER
R-GGA-5696397 Gap-filling DNA repair synthesis and ligation in GG-NER
R-GGA-5696400 Dual Incision in GG-NER
R-GGA-6782135 Dual incision in TC-NER
R-GGA-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-GGA-6783310 Fanconi Anemia Pathway
R-GGA-68962 Activation of the pre-replicative complex
R-GGA-69166 Removal of the Flap Intermediate

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q5ZJJ2

Gene expression databases

BgeeiENSGALG00000003072 Expressed in 11 organ(s), highest expression level in female gonad

Family and domain databases

CDDicd04476 RPA1_DBD_C, 1 hit
cd04477 RPA1N, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR004365 NA-bd_OB_tRNA
IPR013955 Rep_factor-A_C
IPR007199 Rep_factor-A_N
IPR031657 REPA_OB_2
IPR004591 Rfa1
PfamiView protein in Pfam
PF04057 Rep-A_N, 1 hit
PF08646 Rep_fac-A_C, 1 hit
PF16900 REPA_OB_2, 1 hit
PF01336 tRNA_anti-codon, 1 hit
SUPFAMiSSF50249 SSF50249, 4 hits
TIGRFAMsiTIGR00617 rpa1, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRFA1_CHICK
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q5ZJJ2
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 7, 2006
Last sequence update: November 23, 2004
Last modified: January 16, 2019
This is version 101 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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