UniProtKB - Q5XPI4 (RN123_HUMAN)
E3 ubiquitin-protein ligase RNF123
RNF123
Functioni
Catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Promotes the ubiquitination and proteasome-mediated degradation of CDKN1B which is the cyclin-dependent kinase inhibitor at the G0-G1 transition of the cell cycle (PubMed:15531880, PubMed:16227581).
Functions also as an inhibitor of innate antiviral signaling mediated by DDX58 and IFIH1 independently of its E3 ligase activity (PubMed:27312109).
Interacts with the N-terminal CARD domains of DDX58 and IFIH1 and competes with the downstream adapter MAVS (PubMed:27312109).
3 PublicationsCatalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine. EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1254 – 1292 | RING-typePROSITE-ProRule annotationAdd BLAST | 39 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin-protein transferase activity Source: GO_Central
GO - Biological processi
- proteolysis involved in cellular protein catabolic process Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q5XPI4 |
Reactomei | R-HSA-5689880, Ub-specific processing proteases R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q5XPI4 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase RNF123 (EC:2.3.2.27)Alternative name(s): Kip1 ubiquitination-promoting complex protein 1 RING finger protein 123 RING-type E3 ubiquitin transferase RNF123Curated |
Gene namesi | Name:RNF123 Synonyms:KPC1 ORF Names:FP1477 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:21148, RNF123 |
MIMi | 614472, gene |
neXtProti | NX_Q5XPI4 |
VEuPathDBi | HostDB:ENSG00000164068 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 2 Publications
Cytosol
- cytosol Source: HPA
Nucleus
- nuclear membrane Source: HPA
Other locations
- cytoplasm Source: LIFEdb
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 63891 |
OpenTargetsi | ENSG00000164068 |
PharmGKBi | PA134916827 |
Miscellaneous databases
Pharosi | Q5XPI4, Tbio |
Genetic variation databases
BioMutai | RNF123 |
DMDMi | 74748090 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000250447 | 2 – 1314 | E3 ubiquitin-protein ligase RNF123Add BLAST | 1313 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 | |
Modified residuei | 675 | PhosphoserineBy similarity | 1 | |
Modified residuei | 683 | Asymmetric dimethylarginineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q5XPI4 |
jPOSTi | Q5XPI4 |
MassIVEi | Q5XPI4 |
MaxQBi | Q5XPI4 |
PaxDbi | Q5XPI4 |
PeptideAtlasi | Q5XPI4 |
PRIDEi | Q5XPI4 |
ProteomicsDBi | 65842 [Q5XPI4-1] 65843 [Q5XPI4-2] |
PTM databases
iPTMneti | Q5XPI4 |
PhosphoSitePlusi | Q5XPI4 |
Expressioni
Gene expression databases
Bgeei | ENSG00000164068, Expressed in muscle of leg and 224 other tissues |
ExpressionAtlasi | Q5XPI4, baseline and differential |
Genevisiblei | Q5XPI4, HS |
Organism-specific databases
HPAi | ENSG00000164068, Tissue enhanced (skeletal muscle, tongue) |
Interactioni
Subunit structurei
Component of the KPC complex composed of RNF123/KPC1 and UBAC1/KPC2.
Interacts with UBAC1 and CDKN1B via its N-terminal domain.
Interacts with DDX58 (via N-terminus) and IFIH1 (via N-terminus).
3 PublicationsProtein-protein interaction databases
BioGRIDi | 121971, 805 interactors |
CORUMi | Q5XPI4 |
IntActi | Q5XPI4, 36 interactors |
MINTi | Q5XPI4 |
STRINGi | 9606.ENSP00000328287 |
Miscellaneous databases
RNActi | Q5XPI4, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q5XPI4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 74 – 254 | B30.2/SPRYPROSITE-ProRule annotationAdd BLAST | 181 |
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 1254 – 1292 | RING-typePROSITE-ProRule annotationAdd BLAST | 39 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2242, Eukaryota KOG4692, Eukaryota |
GeneTreei | ENSGT00940000155781 |
HOGENOMi | CLU_006687_2_0_1 |
InParanoidi | Q5XPI4 |
OMAi | NHQHVMG |
OrthoDBi | 54386at2759 |
PhylomeDBi | Q5XPI4 |
TreeFami | TF313546 |
Family and domain databases
CDDi | cd12882, SPRY_RNF123, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR013320, ConA-like_dom_sf IPR045129, RNF123/RSPRY1-like IPR003877, SPRY_dom IPR035773, SPRY_RNF123 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR13363, PTHR13363, 1 hit |
Pfami | View protein in Pfam PF00622, SPRY, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50089, ZF_RING_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASKGAGMSF SRKSYRLTSD AEKSRVTGIV QEKLLNDYLN RIFSSSEHAP
60 70 80 90 100
PAATSRKPLN FQNLPEHLDQ LLQVDNEEEE SQGQVEGRLG PSTVVLDHTG
110 120 130 140 150
GFEGLLLVDD DLLGVIGHSN FGTIRSTTCV YKGKWLYEVL ISSQGLMQIG
160 170 180 190 200
WCTISCRFNQ EEGVGDTHNS YAYDGNRVRK WNVTTTNYGK AWAAGDIVSC
210 220 230 240 250
LIDLDDGTLS FCLNGVSLGT AFENLSRGLG MAYFPAISLS FKESVAFNFG
260 270 280 290 300
SRPLRYPVAG YRPLQDPPSA DLVRAQRLLG CFRAVLSVEL DPVEGRLLDK
310 320 330 340 350
ESSKWRLRGQ PTVLLTLAHI FHHFAPLLRK VYLVEAVLMS FLLGIVEKGT
360 370 380 390 400
PTQAQSVVHQ VLDLLWLFME DYEVQDCLKQ LMMSLLRLYR FSPIVPDLGL
410 420 430 440 450
QIHYLRLTIA ILRHEKSRKF LLSNVLFDVL RSVVFFYIKS PLRVEEAGLQ
460 470 480 490 500
ELIPTTWWPH CSSREGKEST EMKEETAEER LRRRAYERGC QRLRKRIEVV
510 520 530 540 550
EELQVQILKL LLDNKDDNGG EASRYIFLTK FRKFLQENAS GRGNMPMLCP
560 570 580 590 600
PEYMVCFLHR LISALRYYWD EYKASNPHAS FSEEAYIPPQ VFYNGKVDYF
610 620 630 640 650
DLQRLGGLLS HLRKTLKDDL ASKANIVIDP LELQSTAMDD LDEDEEPAPA
660 670 680 690 700
MAQRPMQALA VGGPLPLPRP GWLSSPTLGR ANRFLSTAAV SLMTPRRPLS
710 720 730 740 750
TSEKVKVRTL SVEQRTREDI EGSHWNEGLL LGRPPEEPEQ PLTENSLLEV
760 770 780 790 800
LDGAVMMYNL SVHQQLGKMV GVSDDVNEYA MALRDTEDKL RRCPKRRKDI
810 820 830 840 850
LAELTKSQKV FSEKLDHLSR RLAWVHATVY SQEKMLDIYW LLRVCLRTIE
860 870 880 890 900
HGDRTGSLFA FMPEFYLSVA INSYSALKNY FGPVHSMEEL PGYEETLTRL
910 920 930 940 950
AAILAKHFAD ARIVGTDIRD SLMQALASYV CYPHSLRAVE RIPEEQRIAM
960 970 980 990 1000
VRNLLAPYEQ RPWAQTNWIL VRLWRGCGFG YRYTRLPHLL KTKLEDANLP
1010 1020 1030 1040 1050
SLQKPCPSTL LQQHMADLLQ QGPDVAPSFL NSVLNQLNWA FSEFIGMIQE
1060 1070 1080 1090 1100
IQQAAERLER NFVDSRQLKV CATCFDLSVS LLRVLEMTIT LVPEIFLDWT
1110 1120 1130 1140 1150
RPTSEMLLRR LAQLLNQVLN RVTAERNLFD RVVTLRLPGL ESVDHYPILV
1160 1170 1180 1190 1200
AVTGILVQLL VRGPASEREQ ATSVLLADPC FQLRSICYLL GQPEPPAPGT
1210 1220 1230 1240 1250
ALPAPDRKRF SLQSYADYIS ADELAQVEQM LAHLTSASAQ AAAASLPTSE
1260 1270 1280 1290 1300
EDLCPICYAH PISAVFQPCG HKSCKACINQ HLMNNKDCFF CKTTIVSVED
1310
WEKGANTSTT SSAA
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9J266 | C9J266_HUMAN | E3 ubiquitin-protein ligase RNF123 | RNF123 | 762 | Annotation score: | ||
C9JS59 | C9JS59_HUMAN | E3 ubiquitin-protein ligase RNF123 | RNF123 | 721 | Annotation score: | ||
C9JI97 | C9JI97_HUMAN | E3 ubiquitin-protein ligase RNF123 | RNF123 | 426 | Annotation score: | ||
C9JN91 | C9JN91_HUMAN | E3 ubiquitin-protein ligase RNF123 | RNF123 | 76 | Annotation score: | ||
F8WB91 | F8WB91_HUMAN | E3 ubiquitin-protein ligase RNF123 | RNF123 | 68 | Annotation score: | ||
H7C3U1 | H7C3U1_HUMAN | E3 ubiquitin-protein ligase RNF123 | RNF123 | 25 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 809 | K → R in BAB14139 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_027561 | 51 | P → R. Corresponds to variant dbSNP:rs2960546Ensembl. | 1 | |
Natural variantiVAR_052106 | 387 | R → Q. Corresponds to variant dbSNP:rs35620248Ensembl. | 1 | |
Natural variantiVAR_052107 | 596 | K → E. Corresponds to variant dbSNP:rs35726701Ensembl. | 1 | |
Natural variantiVAR_052108 | 854 | R → H2 PublicationsCorresponds to variant dbSNP:rs34823813Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_020650 | 1295 – 1314 | IVSVE…TSSAA → TSNLLACLYPHWWEPSHGPN CA in isoform 2. 1 PublicationAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY744152 mRNA Translation: AAU93470.1 AC099668 Genomic DNA No translation available. BC041145 mRNA Translation: AAH41145.1 BC057392 mRNA Translation: AAH57392.2 BC088801 mRNA Translation: AAH88801.1 BC130632 mRNA Translation: AAI30633.1 AF370367 mRNA Translation: AAQ15203.1 AK022627 mRNA Translation: BAB14139.1 Different initiation. AK026968 mRNA Translation: BAB15607.1 Different initiation. AL136729 mRNA Translation: CAB66663.2 |
CCDSi | CCDS33758.1 [Q5XPI4-1] |
RefSeqi | NP_071347.2, NM_022064.4 [Q5XPI4-1] XP_006713348.1, XM_006713285.1 [Q5XPI4-1] XP_011532297.1, XM_011533995.1 [Q5XPI4-1] XP_016862507.1, XM_017007018.1 [Q5XPI4-1] |
Genome annotation databases
Ensembli | ENST00000327697; ENSP00000328287; ENSG00000164068 |
GeneIDi | 63891 |
KEGGi | hsa:63891 |
MANE-Selecti | ENST00000327697.11; ENSP00000328287.6; NM_022064.5; NP_071347.2 |
UCSCi | uc003cxh.5, human [Q5XPI4-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY744152 mRNA Translation: AAU93470.1 AC099668 Genomic DNA No translation available. BC041145 mRNA Translation: AAH41145.1 BC057392 mRNA Translation: AAH57392.2 BC088801 mRNA Translation: AAH88801.1 BC130632 mRNA Translation: AAI30633.1 AF370367 mRNA Translation: AAQ15203.1 AK022627 mRNA Translation: BAB14139.1 Different initiation. AK026968 mRNA Translation: BAB15607.1 Different initiation. AL136729 mRNA Translation: CAB66663.2 |
CCDSi | CCDS33758.1 [Q5XPI4-1] |
RefSeqi | NP_071347.2, NM_022064.4 [Q5XPI4-1] XP_006713348.1, XM_006713285.1 [Q5XPI4-1] XP_011532297.1, XM_011533995.1 [Q5XPI4-1] XP_016862507.1, XM_017007018.1 [Q5XPI4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2MA6 | NMR | - | A | 1247-1304 | [»] | |
SMRi | Q5XPI4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 121971, 805 interactors |
CORUMi | Q5XPI4 |
IntActi | Q5XPI4, 36 interactors |
MINTi | Q5XPI4 |
STRINGi | 9606.ENSP00000328287 |
PTM databases
iPTMneti | Q5XPI4 |
PhosphoSitePlusi | Q5XPI4 |
Genetic variation databases
BioMutai | RNF123 |
DMDMi | 74748090 |
Proteomic databases
EPDi | Q5XPI4 |
jPOSTi | Q5XPI4 |
MassIVEi | Q5XPI4 |
MaxQBi | Q5XPI4 |
PaxDbi | Q5XPI4 |
PeptideAtlasi | Q5XPI4 |
PRIDEi | Q5XPI4 |
ProteomicsDBi | 65842 [Q5XPI4-1] 65843 [Q5XPI4-2] |
Protocols and materials databases
Antibodypediai | 30603, 111 antibodies from 19 providers |
DNASUi | 63891 |
Genome annotation databases
Ensembli | ENST00000327697; ENSP00000328287; ENSG00000164068 |
GeneIDi | 63891 |
KEGGi | hsa:63891 |
MANE-Selecti | ENST00000327697.11; ENSP00000328287.6; NM_022064.5; NP_071347.2 |
UCSCi | uc003cxh.5, human [Q5XPI4-1] |
Organism-specific databases
CTDi | 63891 |
DisGeNETi | 63891 |
GeneCardsi | RNF123 |
HGNCi | HGNC:21148, RNF123 |
HPAi | ENSG00000164068, Tissue enhanced (skeletal muscle, tongue) |
MIMi | 614472, gene |
neXtProti | NX_Q5XPI4 |
OpenTargetsi | ENSG00000164068 |
PharmGKBi | PA134916827 |
VEuPathDBi | HostDB:ENSG00000164068 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2242, Eukaryota KOG4692, Eukaryota |
GeneTreei | ENSGT00940000155781 |
HOGENOMi | CLU_006687_2_0_1 |
InParanoidi | Q5XPI4 |
OMAi | NHQHVMG |
OrthoDBi | 54386at2759 |
PhylomeDBi | Q5XPI4 |
TreeFami | TF313546 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q5XPI4 |
Reactomei | R-HSA-5689880, Ub-specific processing proteases R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q5XPI4 |
Miscellaneous databases
BioGRID-ORCSi | 63891, 70 hits in 1084 CRISPR screens |
ChiTaRSi | RNF123, human |
GeneWikii | RNF123 |
GenomeRNAii | 63891 |
Pharosi | Q5XPI4, Tbio |
PROi | PR:Q5XPI4 |
RNActi | Q5XPI4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000164068, Expressed in muscle of leg and 224 other tissues |
ExpressionAtlasi | Q5XPI4, baseline and differential |
Genevisiblei | Q5XPI4, HS |
Family and domain databases
CDDi | cd12882, SPRY_RNF123, 1 hit |
Gene3Di | 2.60.120.920, 1 hit 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR001870, B30.2/SPRY IPR043136, B30.2/SPRY_sf IPR013320, ConA-like_dom_sf IPR045129, RNF123/RSPRY1-like IPR003877, SPRY_dom IPR035773, SPRY_RNF123 IPR001841, Znf_RING IPR013083, Znf_RING/FYVE/PHD |
PANTHERi | PTHR13363, PTHR13363, 1 hit |
Pfami | View protein in Pfam PF00622, SPRY, 1 hit |
SMARTi | View protein in SMART SM00184, RING, 1 hit SM00449, SPRY, 1 hit |
SUPFAMi | SSF49899, SSF49899, 1 hit |
PROSITEi | View protein in PROSITE PS50188, B302_SPRY, 1 hit PS50089, ZF_RING_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RN123_HUMAN | |
Accessioni | Q5XPI4Primary (citable) accession number: Q5XPI4 Secondary accession number(s): A1L4Q3 Q9H9T2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | November 23, 2004 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references