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Protein

Presenilins-associated rhomboid-like protein, mitochondrial

Gene

Parl

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity).By similarity1 Publication

Catalytic activityi

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei275NucleophileBy similarity1
Active sitei333By similarity1

GO - Molecular functioni

  • endopeptidase activity Source: ParkinsonsUK-UCL
  • serine-type endopeptidase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-8949664 Processing of SMDT1

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilins-associated rhomboid-like protein, mitochondrial (EC:3.4.21.105)
Alternative name(s):
Mitochondrial intramembrane-cleaving protease PARL
Cleaved into the following chain:
P-beta
Short name:
Pbeta
Gene namesi
Name:Parl
Synonyms:Psarl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1277152 Parl

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini51 – 99Mitochondrial matrixSequence analysisAdd BLAST49
Transmembranei100 – 119HelicalSequence analysisAdd BLAST20
Topological domaini120 – 165Mitochondrial intermembraneSequence analysisAdd BLAST46
Transmembranei166 – 185HelicalSequence analysisAdd BLAST20
Topological domaini186 – 205Mitochondrial matrixSequence analysisAdd BLAST20
Transmembranei206 – 228HelicalSequence analysisAdd BLAST23
Topological domaini229 – 242Mitochondrial intermembraneSequence analysisAdd BLAST14
Transmembranei243 – 260HelicalSequence analysisAdd BLAST18
Topological domaini261 – 270Mitochondrial matrixSequence analysis10
Transmembranei271 – 287HelicalSequence analysisAdd BLAST17
Topological domaini288 – 293Mitochondrial intermembraneSequence analysis6
Transmembranei294 – 316HelicalSequence analysisAdd BLAST23
Topological domaini317 – 330Mitochondrial matrixSequence analysisAdd BLAST14
Transmembranei331 – 352HelicalSequence analysisAdd BLAST22
Topological domaini353 – 377Mitochondrial intermembraneSequence analysisAdd BLAST25

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice develop normally until 4 weeks of age. They show subsequent progressive growth retardation, atrophy of muscle, spleen, and thymus as well as severe apoptosis of T and B lymphocytes leading to premature death between 8-12 weeks of age. Mouse embryonic fibroblasts lacking Parl show high susceptibility to intrinsic apoptotic signals. This defect can be complemented by Parl or a soluble form of Opa1.1 Publication

Chemistry databases

ChEMBLiCHEMBL3259502

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50MitochondrionBy similarityAdd BLAST50
ChainiPRO_000002738851 – 377Presenilins-associated rhomboid-like protein, mitochondrialAdd BLAST327
PeptideiPRO_000002738951 – 75P-betaBy similarityAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63PhosphoserineBy similarity1
Modified residuei68PhosphoserineBy similarity1

Post-translational modificationi

P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5XJY4
MaxQBiQ5XJY4
PaxDbiQ5XJY4
PRIDEiQ5XJY4

PTM databases

PhosphoSitePlusiQ5XJY4

Expressioni

Gene expression databases

BgeeiENSMUSG00000033918 Expressed in 79 organ(s), highest expression level in primary oocyte
CleanExiMM_PARL
ExpressionAtlasiQ5XJY4 baseline and differential

Interactioni

Subunit structurei

Interacts with PSEN1 and PSEN2 (By similarity). Binds OPA1.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-59825N
IntActiQ5XJY4, 2 interactors
STRINGi10090.ENSMUSP00000045361

Structurei

3D structure databases

ProteinModelPortaliQ5XJY4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Curated

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2980 Eukaryota
COG0705 LUCA
GeneTreeiENSGT00390000013063
HOGENOMiHOG000230670
HOVERGENiHBG082107
InParanoidiQ5XJY4
KOiK09650
OMAiEQFVAVY
OrthoDBiEOG091G0B3Z
PhylomeDBiQ5XJY4
TreeFamiTF313603

Family and domain databases

Gene3Di1.20.1540.10, 1 hit
InterProiView protein in InterPro
IPR022764 Peptidase_S54_rhomboid_dom
IPR035952 Rhomboid-like_sf
PfamiView protein in Pfam
PF01694 Rhomboid, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

Q5XJY4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MALQGWVQRG WRCGPAWAPP LGGGYRELSA TQAPRLLGRR FNLFVQQKCG
60 70 80 90 100
FRKAPRKVEP RRSDTGSSGE AYKRSALIPP LEETVFYPSP YPIRTLVKPF
110 120 130 140 150
FFTIGFTGCA FGSAAIWQYE SLKSRVQSYF DGIKADWLDS IRPQKEGNLR
160 170 180 190 200
KEINKWWNSL SDGQRTVTGI IAANALVFCL WRVPSLQRTM IRYFTSNPAS
210 220 230 240 250
KVLCSPMLLS TFSHFSLFHM AANMYVLWSF SSSIVNILGQ EQFVAVYLSA
260 270 280 290 300
GVISNFVSYV CKVATGRYGP SLGASGAIMT VLAAVCTKIP EGRLAIIFLP
310 320 330 340 350
VFTFTAGNAL KAIIAMDTAG MILGWKFFDH AAHLGGALFG IWYITYGHEL
360 370
IWKNREPLVK IWHEIRTNGP KKGGGSK
Length:377
Mass (Da):41,964
Last modified:November 23, 2004 - v1
Checksum:i41C81310031A2EC4
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A338P6U4A0A338P6U4_MOUSE
Presenilins-associated rhomboid-lik...
Parl
343Annotation score:
A0A338P6A0A0A338P6A0_MOUSE
Presenilins-associated rhomboid-lik...
Parl
188Annotation score:
A0A338P759A0A338P759_MOUSE
Presenilins-associated rhomboid-lik...
Parl
68Annotation score:
D6RJ50D6RJ50_MOUSE
Presenilins-associated rhomboid-lik...
Parl
171Annotation score:
D6RCZ6D6RCZ6_MOUSE
Presenilins-associated rhomboid-lik...
Parl
119Annotation score:
A0A338P7G5A0A338P7G5_MOUSE
Presenilins-associated rhomboid-lik...
Parl
103Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK151652 mRNA Translation: BAE30581.1
BC083153 mRNA Translation: AAH83153.1
CCDSiCCDS28044.1
RefSeqiNP_001005767.1, NM_001005767.4
UniGeneiMm.371737

Genome annotation databases

EnsembliENSMUST00000048642; ENSMUSP00000045361; ENSMUSG00000033918
GeneIDi381038
KEGGimmu:381038
UCSCiuc007ypm.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK151652 mRNA Translation: BAE30581.1
BC083153 mRNA Translation: AAH83153.1
CCDSiCCDS28044.1
RefSeqiNP_001005767.1, NM_001005767.4
UniGeneiMm.371737

3D structure databases

ProteinModelPortaliQ5XJY4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59825N
IntActiQ5XJY4, 2 interactors
STRINGi10090.ENSMUSP00000045361

Chemistry databases

ChEMBLiCHEMBL3259502

PTM databases

PhosphoSitePlusiQ5XJY4

Proteomic databases

EPDiQ5XJY4
MaxQBiQ5XJY4
PaxDbiQ5XJY4
PRIDEiQ5XJY4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048642; ENSMUSP00000045361; ENSMUSG00000033918
GeneIDi381038
KEGGimmu:381038
UCSCiuc007ypm.1 mouse

Organism-specific databases

CTDi55486
MGIiMGI:1277152 Parl

Phylogenomic databases

eggNOGiKOG2980 Eukaryota
COG0705 LUCA
GeneTreeiENSGT00390000013063
HOGENOMiHOG000230670
HOVERGENiHBG082107
InParanoidiQ5XJY4
KOiK09650
OMAiEQFVAVY
OrthoDBiEOG091G0B3Z
PhylomeDBiQ5XJY4
TreeFamiTF313603

Enzyme and pathway databases

ReactomeiR-MMU-8949664 Processing of SMDT1

Miscellaneous databases

PROiPR:Q5XJY4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033918 Expressed in 79 organ(s), highest expression level in primary oocyte
CleanExiMM_PARL
ExpressionAtlasiQ5XJY4 baseline and differential

Family and domain databases

Gene3Di1.20.1540.10, 1 hit
InterProiView protein in InterPro
IPR022764 Peptidase_S54_rhomboid_dom
IPR035952 Rhomboid-like_sf
PfamiView protein in Pfam
PF01694 Rhomboid, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPARL_MOUSE
AccessioniPrimary (citable) accession number: Q5XJY4
Secondary accession number(s): Q3U9T5, Q641T5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: November 23, 2004
Last modified: November 7, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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