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Protein

Glutathione synthetase

Gene

gss

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Unreviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: glutathione biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes glutathione from L-cysteine and L-glutamate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glutathione synthetase (gss), Glutathione synthetase (gss), Glutathione synthetase (gss)
This subpathway is part of the pathway glutathione biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes glutathione from L-cysteine and L-glutamate, the pathway glutathione biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei125SubstrateUniRule annotation1
Metal bindingi144MagnesiumUniRule annotation1
Binding sitei144ATPUniRule annotation1
Metal bindingi146MagnesiumUniRule annotation1
Binding sitei306ATPUniRule annotation1
Metal bindingi369MagnesiumUniRule annotation1
Binding sitei376ATPUniRule annotation1
Binding sitei426ATPUniRule annotation1
Binding sitei451SubstrateUniRule annotation1
Binding sitei453ATPUniRule annotation1
Binding sitei459ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi365 – 374ATPUniRule annotation10
Nucleotide bindingi399 – 402ATPUniRule annotation4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-UniRule
  • glutathione binding Source: GO_Central
  • glutathione synthase activity Source: GO_Central
  • magnesium ion binding Source: UniProtKB-UniRule
  • protein homodimerization activity Source: UniProtKB-UniRule

Keywordsi

Molecular functionLigaseUniRule annotation
Biological processGlutathione biosynthesisUniRule annotation
LigandATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayi
UPA00142;UER00210

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione synthetaseUniRule annotation (EC:6.3.2.3UniRule annotation)
Short name:
GSH-SUniRule annotation
Gene namesi
Name:gssImported
Synonyms:zgc:101574Imported
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio

Organism-specific databases

ZFINiZDB-GENE-041010-208 gss

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Interactioni

GO - Molecular functioni

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini205 – 303GSH_synthaseInterPro annotationAdd BLAST99

Sequence similaritiesi

Belongs to the eukaryotic GSH synthase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG0021 Eukaryota
ENOG410XPHH LUCA
HOGENOMiHOG000172641
HOVERGENiHBG002458
KOiK21456

Family and domain databases

Gene3Di1.10.1080.10, 1 hit
3.30.1490.50, 1 hit
3.30.1490.80, 1 hit
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit

Sequencei

Sequence statusi: Complete.

Q5XJT8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVKLEDTLK DENLIKRLEE IAKDTALLHG VLMRTKDTPN SPEVVSYAPF
60 70 80 90 100
TLFPTSVPAA LFHQALAVQT QFNRLVDRVS QNHSFLEDTL ASTIKVDDFT
110 120 130 140 150
ARLFNIYKQV QQEGHAQKIV VGLNRSDYML DHSPDGRTSL KQIEINTIAA
160 170 180 190 200
SFGGLASRTP DVHRHILKVA NLPDECSLVL DNNPAAGLAK GLAKAWELYG
210 220 230 240 250
SKRAVVLFLV ENVQRNIYDH RYVENELWKR NIPVIRRQFE DVFRTGSLDE
260 270 280 290 300
SKRLFVDGHE VAIVYFRNGY MPQNYTSEQS WEVRLMMERS VAVKCPDIST
310 320 330 340 350
HLAGTKEVQQ ELARPGVLEC FFPDEPETVS QIRATFAGLY TLDMGEEGDN
360 370 380 390 400
TVAMALANPD QYVLKPQREG GGNNIYGSEI CEVLEKLKNS SERTAYILMD
410 420 430 440 450
KIQPVPVQNI LLRPGAPLKV SSCLSELGAF GAYVRKGSEL VLNECVGHLL
460 470
RTKSSEHADG GVAAGVAVLD NPLLV
Length:475
Mass (Da):52,895
Last modified:November 23, 2004 - v1
Checksum:i2C285AE03B14E432
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083207 mRNA Translation: AAH83207.1
BC165747 mRNA Translation: AAI65747.1
RefSeqiNP_001006104.1, NM_001006104.1
UniGeneiDr.88110

Genome annotation databases

GeneIDi450084
KEGGidre:450084

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083207 mRNA Translation: AAH83207.1
BC165747 mRNA Translation: AAI65747.1
RefSeqiNP_001006104.1, NM_001006104.1
UniGeneiDr.88110

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi450084
KEGGidre:450084

Organism-specific databases

CTDi2937
ZFINiZDB-GENE-041010-208 gss

Phylogenomic databases

eggNOGiKOG0021 Eukaryota
ENOG410XPHH LUCA
HOGENOMiHOG000172641
HOVERGENiHBG002458
KOiK21456

Enzyme and pathway databases

UniPathwayi
UPA00142;UER00210

Family and domain databases

Gene3Di1.10.1080.10, 1 hit
3.30.1490.50, 1 hit
3.30.1490.80, 1 hit
3.40.50.1760, 1 hit
InterProiView protein in InterPro
IPR005615 Glutathione_synthase
IPR014042 Glutathione_synthase_a-hlx
IPR014709 Glutathione_synthase_C_euk
IPR014049 Glutathione_synthase_N_euk
IPR037013 GSH-S_sub-bd_sf
IPR004887 GSH_synth_subst-bd
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR11130 PTHR11130, 1 hit
PfamiView protein in Pfam
PF03917 GSH_synth_ATP, 1 hit
PF03199 GSH_synthase, 1 hit
PIRSFiPIRSF001558 GSHase, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01986 glut_syn_euk, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiQ5XJT8_DANRE
AccessioniPrimary (citable) accession number: Q5XJT8
Entry historyiIntegrated into UniProtKB/TrEMBL: November 23, 2004
Last sequence update: November 23, 2004
Last modified: November 7, 2018
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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