UniProtKB - Q5XI64 (ABHD6_RAT)
Protein
Monoacylglycerol lipase ABHD6
Gene
Abhd6
Organism
Rattus norvegicus (Rat)
Status
Functioni
Lipase that preferentially hydrolysis medium-chain saturated monoacylglycerols including 2-arachidonoylglycerol (By similarity). Through 2-arachidonoylglycerol degradation may regulate endocannabinoid signaling pathways. Also has a lysophosphatidyl lipase activity with a preference for lysophosphatidylglycerol among other lysophospholipids (By similarity). Also able to degrade bis(monoacylglycero)phosphate (BMP) and constitutes the major enzyme for BMP catabolism. BMP, also known as lysobisphosphatidic acid, is enriched in late endosomes and lysosomes and plays a key role in the formation of intraluminal vesicles and in lipid sorting (By similarity).By similarity
Catalytic activityi
- 2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H+By similarity
- 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-glycerol + H2O = (5Z,8Z,11Z,14Z)-eicosatetraenoate + glycerol + H+By similarity
- Hydrolyzes glycerol monoesters of long-chain fatty acids.By similarity EC:3.1.1.23
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 148 | NucleophileBy similarity | 1 | |
Active sitei | 278 | Charge relay systemBy similarity | 1 | |
Active sitei | 306 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- acylglycerol lipase activity Source: UniProtKB
- phospholipase activity Source: RGD
GO - Biological processi
- acylglycerol catabolic process Source: UniProtKB
- long-term synaptic depression Source: RGD
- lysobisphosphatidic acid metabolic process Source: UniProtKB
- monoacylglycerol catabolic process Source: UniProtKB
- negative regulation of cell migration Source: RGD
- negative regulation of cold-induced thermogenesis Source: YuBioLab
- phospholipid catabolic process Source: RGD
- positive regulation of lipid biosynthetic process Source: RGD
- regulation of endocannabinoid signaling pathway Source: RGD
- regulation of retrograde trans-synaptic signaling by endocanabinoid Source: RGD
Keywordsi
Molecular function | Hydrolase |
Biological process | Lipid metabolism |
Enzyme and pathway databases
Reactomei | R-RNO-426048, Arachidonate production from DAG |
Protein family/group databases
ESTHERi | rat-abhd6, ABHD6-Lip |
Names & Taxonomyi
Protein namesi | Recommended name: Monoacylglycerol lipase ABHD6Curated (EC:3.1.1.23By similarity)Alternative name(s): 2-arachidonoylglycerol hydrolaseBy similarity Abhydrolase domain-containing protein 6Imported |
Gene namesi | Name:Abhd6Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1359323, Abhd6 |
Subcellular locationi
Lysosome
- Lysosome membrane By similarity; Single-pass type II membrane protein Sequence analysis
Mitochondrion
- Mitochondrion membrane By similarity; Single-pass type II membrane protein Sequence analysis
Endosome
- Late endosome membrane By similarity; Single-pass type II membrane protein Sequence analysis
Endosome
- late endosome membrane Source: UniProtKB
Lysosome
- lysosomal membrane Source: UniProtKB
Mitochondrion
- mitochondrial membrane Source: UniProtKB-SubCell
- mitochondrion Source: RGD
Plasma Membrane
- AMPA glutamate receptor complex Source: RGD
- integral component of postsynaptic membrane Source: RGD
Other locations
- GABA-ergic synapse Source: RGD
- glutamatergic synapse Source: RGD
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 8 | ExtracellularSequence analysis | 8 | |
Transmembranei | 9 – 29 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 30 – 337 | CytoplasmicSequence analysisAdd BLAST | 308 |
Keywords - Cellular componenti
Endosome, Lysosome, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281577 | 1 – 337 | Monoacylglycerol lipase ABHD6Add BLAST | 337 |
Proteomic databases
jPOSTi | Q5XI64 |
PaxDbi | Q5XI64 |
PRIDEi | Q5XI64 |
PTM databases
PhosphoSitePlusi | Q5XI64 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000008167, Expressed in jejunum and 21 other tissues |
Genevisiblei | Q5XI64, RN |
Family & Domainsi
Sequence similaritiesi
Belongs to the AB hydrolase superfamily.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1454, Eukaryota |
GeneTreei | ENSGT00510000047225 |
InParanoidi | Q5XI64 |
OMAi | AYYPSDI |
OrthoDBi | 1285824at2759 |
PhylomeDBi | Q5XI64 |
TreeFami | TF331946 |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000073, AB_hydrolase_1 IPR000639, Epox_hydrolase-like |
Pfami | View protein in Pfam PF12697, Abhydrolase_6, 1 hit |
PRINTSi | PR00111, ABHYDROLASE PR00412, EPOXHYDRLASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5XI64-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLDVVNMFV IAGGTLAIPI LAFVASFLLW PSALIRIYYW YWRRTLGMQV
60 70 80 90 100
RYVHHEDYQF CYSFRGRPGH KPSVLMLHGF SAHKDMWLSV VKFLPKNLHL
110 120 130 140 150
VCVDMPGHEG TTRSSLDDLS IVGQVKRIHQ FVECLKLNKK PFHLIGTSMG
160 170 180 190 200
GNVAGVYAAY YPSDVCSLSL VCPAGLQYST DNRFVQRLKE LEDSAATQKI
210 220 230 240 250
PLIPSTPEEM SEMLQLCSYV RFKVPQQILQ GLVDVRIPHN SFYRKLFLEI
260 270 280 290 300
VSEKSRYSLH ENMDKIKVPT QIIWGKQDQV LDVSGADILA KSITNSQVEV
310 320 330
LENCGHSVVM ERPRKTAKLV VDFLASVHNP DNNKKLN
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC083826 mRNA Translation: AAH83826.1 |
RefSeqi | NP_001007681.1, NM_001007680.1 XP_006251828.1, XM_006251766.3 XP_006251829.1, XM_006251767.3 XP_006251830.1, XM_006251768.3 |
Genome annotation databases
Ensembli | ENSRNOT00000012271; ENSRNOP00000012271; ENSRNOG00000008167 ENSRNOT00000080794; ENSRNOP00000074573; ENSRNOG00000008167 |
GeneIDi | 305795 |
KEGGi | rno:305795 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC083826 mRNA Translation: AAH83826.1 |
RefSeqi | NP_001007681.1, NM_001007680.1 XP_006251828.1, XM_006251766.3 XP_006251829.1, XM_006251767.3 XP_006251830.1, XM_006251768.3 |
3D structure databases
SMRi | Q5XI64 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | Q5XI64 |
STRINGi | 10116.ENSRNOP00000012271 |
Chemistry databases
ChEMBLi | CHEMBL3708569 |
Protein family/group databases
ESTHERi | rat-abhd6, ABHD6-Lip |
PTM databases
PhosphoSitePlusi | Q5XI64 |
Proteomic databases
jPOSTi | Q5XI64 |
PaxDbi | Q5XI64 |
PRIDEi | Q5XI64 |
Genome annotation databases
Ensembli | ENSRNOT00000012271; ENSRNOP00000012271; ENSRNOG00000008167 ENSRNOT00000080794; ENSRNOP00000074573; ENSRNOG00000008167 |
GeneIDi | 305795 |
KEGGi | rno:305795 |
Organism-specific databases
CTDi | 57406 |
RGDi | 1359323, Abhd6 |
Phylogenomic databases
eggNOGi | KOG1454, Eukaryota |
GeneTreei | ENSGT00510000047225 |
InParanoidi | Q5XI64 |
OMAi | AYYPSDI |
OrthoDBi | 1285824at2759 |
PhylomeDBi | Q5XI64 |
TreeFami | TF331946 |
Enzyme and pathway databases
Reactomei | R-RNO-426048, Arachidonate production from DAG |
Miscellaneous databases
PROi | PR:Q5XI64 |
Gene expression databases
Bgeei | ENSRNOG00000008167, Expressed in jejunum and 21 other tissues |
Genevisiblei | Q5XI64, RN |
Family and domain databases
Gene3Di | 3.40.50.1820, 1 hit |
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR000073, AB_hydrolase_1 IPR000639, Epox_hydrolase-like |
Pfami | View protein in Pfam PF12697, Abhydrolase_6, 1 hit |
PRINTSi | PR00111, ABHYDROLASE PR00412, EPOXHYDRLASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ABHD6_RAT | |
Accessioni | Q5XI64Primary (citable) accession number: Q5XI64 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | November 23, 2004 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families