UniProtKB - Q5XI01 (LARP7_RAT)
Protein
La-related protein 7
Gene
Larp7
Organism
Rattus norvegicus (Rat)
Status
Functioni
RNA-binding protein that specifically binds distinct small nuclear RNA (snRNAs) and regulates their processing and function. Specifically binds the 7SK snRNA (7SK RNA) and acts as a core component of the 7SK ribonucleoprotein (RNP) complex, thereby acting as a negative regulator of transcription elongation by RNA polymerase II. The 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC). LARP7 specifically binds to the highly conserved 3'-terminal U-rich stretch of 7SK RNA; on stimulation, remains associated with 7SK RNA, whereas P-TEFb is released from the complex. LARP7 also acts as a regulator of mRNA splicing fidelity by promoting U6 snRNA processing. Specifically binds U6 snRNAs and associates with a subset of box C/D RNP complexes: promotes U6 snRNA 2'-O-methylation by facilitating U6 snRNA loading into box C/D RNP complexes. U6 snRNA 2'-O-methylation is required for mRNA splicing fidelity. Binds U6 snRNAs with a 5'-CAGGG-3' sequence motif (By similarity). U6 snRNA processing is required for spermatogenesis (By similarity).By similarity
GO - Molecular functioni
- 7SK snRNA binding Source: UniProtKB
- U6 snRNA binding Source: UniProtKB
GO - Biological processi
- box C/D RNA 3'-end processing Source: UniProtKB
- cell differentiation Source: UniProtKB-KW
- germ cell proliferation Source: RGD
- mRNA processing Source: UniProtKB-KW
- negative regulation of chromatin binding Source: RGD
- negative regulation of transcription by RNA polymerase II Source: RGD
- negative regulation of viral transcription Source: RGD
- positive regulation of G1/S transition of mitotic cell cycle Source: RGD
- positive regulation of protein localization to Cajal body Source: UniProtKB
- positive regulation of snRNA transcription by RNA polymerase II Source: UniProtKB
- regulation of mRNA splicing, via spliceosome Source: UniProtKB
- RNA splicing Source: UniProtKB-KW
- spermatogenesis Source: UniProtKB
- U6 2'-O-snRNA methylation Source: UniProtKB
Keywordsi
Molecular function | RNA-binding |
Biological process | Differentiation, mRNA processing, mRNA splicing, Spermatogenesis, Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: La-related protein 7By similarityAlternative name(s): La ribonucleoprotein domain family member 7By similarity |
Gene namesi | Name:Larp7Imported |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1592474, Larp7 |
Subcellular locationi
Nucleus
- nucleoplasm By similarity
Nucleus
- 7SK snRNP Source: RGD
- nucleoplasm Source: UniProtKB-SubCell
- nucleus Source: UniProtKB
Other locations
- ribonucleoprotein complex Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281679 | 1 – 571 | La-related protein 7Add BLAST | 571 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Cross-linki | 232 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 252 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 254 | PhosphoserineCombined sources | 1 | |
Modified residuei | 257 | PhosphoserineCombined sources | 1 | |
Modified residuei | 261 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 294 | PhosphoserineBy similarity | 1 | |
Modified residuei | 295 | PhosphoserineBy similarity | 1 | |
Modified residuei | 335 | PhosphoserineBy similarity | 1 | |
Modified residuei | 336 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 349 | PhosphoserineBy similarity | 1 | |
Cross-linki | 408 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
jPOSTi | Q5XI01 |
PaxDbi | Q5XI01 |
PRIDEi | Q5XI01 |
PTM databases
iPTMneti | Q5XI01 |
PhosphoSitePlusi | Q5XI01 |
Interactioni
Subunit structurei
Core component of the 7SK RNP complex, at least composed of 7SK RNA, LARP7, MEPCE, HEXIM1 (or HEXIM2) and P-TEFb (composed of CDK9 and CCNT1/cyclin-T1).
Interacts with METTL16.
Interacts with RBM7; upon genotoxic stress this interaction is enhanced, triggering the release of inactive P-TEFb complex from the core, yielding to P-TEFb complex activation. Associates with box C/D small nucleolar ribonucleoprotein (snoRNP) complexes.
By similarityProtein-protein interaction databases
BioGRIDi | 601734, 2 interactors |
STRINGi | 10116.ENSRNOP00000065357 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 23 – 117 | HTH La-type RNA-bindingPROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 120 – 198 | RRMPROSITE-ProRule annotationAdd BLAST | 79 | |
Domaini | 439 – 552 | xRRMPROSITE-ProRule annotationAdd BLAST | 114 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 197 – 366 | Lys-richAdd BLAST | 170 |
Domaini
The xRRM domain binds the 3' end of 7SK snRNA (7SK RNA) at the top of stem-loop 4.By similarity
Sequence similaritiesi
Belongs to the LARP7 family.Curated
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
InParanoidi | Q5XI01 |
OrthoDBi | 1499213at2759 |
PhylomeDBi | Q5XI01 |
Family and domain databases
CDDi | cd08032, LARP_7, 1 hit cd12290, RRM1_LARP7, 1 hit cd12542, RRM2_LARP7, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR006630, La_HTH IPR014886, La_RRM IPR034946, LARP7_La IPR034887, LARP7_RRM1 IPR034910, LARP7_RRM2 IPR002344, Lupus_La IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF05383, La, 1 hit PF00076, RRM_1, 1 hit PF08777, RRM_3, 1 hit |
PRINTSi | PR00302, LUPUSLA |
SMARTi | View protein in SMART SM00715, LA, 1 hit SM00360, RRM, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50961, HTH_LA, 1 hit PS50102, RRM, 1 hit PS51939, XRRM, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q5XI01-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
METENQKTME ESTEKRKEEK KKRSRVKQVL ADIAKQVDFW FGDANLHKDR
60 70 80 90 100
FLREQIEKSR DGYVDISLLV SFNKMKKLTT DGKLIARALK SSSVVELDLE
110 120 130 140 150
GTRIRRKKPL GERPKDEEER TVYVELLPKN VTHSWIERVF GKCGNVVYIS
160 170 180 190 200
IPHYKSTGDP KGFAFVEFET KEQAAKAIEF LNNPPEEAPR KPGIFPKTVK
210 220 230 240 250
NKPIPSLRVA EEKKKKKKKK GRIKKEESVQ AKELVVDSSS SGVSKATKRP
260 270 280 290 300
RTASEGSEAE TPEAPKQPAK KKKKRDKVET GGLPESKAGK RERSSAEDED
310 320 330 340 350
CLPPRPKLKK RAQKDGGGPA ASEVSKEHRD LEFCSTEEEK EPGDRKGDSL
360 370 380 390 400
SKGKRKHKKK HKERHKMGEE VIPLRVLSKT EWMDLKKEYL ALQKASMASL
410 420 430 440 450
KKTISQIKLE SEMETESKAP PGSGQQCSTQ EKVSAQGPQF VTGVIVKILS
460 470 480 490 500
EDPLPGRKQV KDILATISEV VYIDLLEGDT ECHARFKTPE DAQAVMNAQT
510 520 530 540 550
EIKKKHSWNL EILSGDHEQR YWQKILVDRQ AKLNQPREKK RGTEKLITKA
560 570
EKIRLAKTQQ ASQHIRFSEY D
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM0R7D1 | M0R7D1_RAT | La ribonucleoprotein domain family ... | Larp7 | 570 | Annotation score: | ||
M0RC12 | M0RC12_RAT | La ribonucleoprotein domain family ... | Larp7 | 389 | Annotation score: |
Sequence cautioni
The sequence AAH83898 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC083898 mRNA Translation: AAH83898.1 Different initiation. |
RefSeqi | NP_001037755.2, NM_001044290.2 |
Genome annotation databases
GeneIDi | 686883 |
KEGGi | rno:686883 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC083898 mRNA Translation: AAH83898.1 Different initiation. |
RefSeqi | NP_001037755.2, NM_001044290.2 |
3D structure databases
SMRi | Q5XI01 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 601734, 2 interactors |
STRINGi | 10116.ENSRNOP00000065357 |
PTM databases
iPTMneti | Q5XI01 |
PhosphoSitePlusi | Q5XI01 |
Proteomic databases
jPOSTi | Q5XI01 |
PaxDbi | Q5XI01 |
PRIDEi | Q5XI01 |
Genome annotation databases
GeneIDi | 686883 |
KEGGi | rno:686883 |
Organism-specific databases
CTDi | 51574 |
RGDi | 1592474, Larp7 |
Phylogenomic databases
eggNOGi | KOG0118, Eukaryota |
InParanoidi | Q5XI01 |
OrthoDBi | 1499213at2759 |
PhylomeDBi | Q5XI01 |
Miscellaneous databases
PROi | PR:Q5XI01 |
Family and domain databases
CDDi | cd08032, LARP_7, 1 hit cd12290, RRM1_LARP7, 1 hit cd12542, RRM2_LARP7, 1 hit |
Gene3Di | 1.10.10.10, 1 hit 3.30.70.330, 2 hits |
InterProi | View protein in InterPro IPR006630, La_HTH IPR014886, La_RRM IPR034946, LARP7_La IPR034887, LARP7_RRM1 IPR034910, LARP7_RRM2 IPR002344, Lupus_La IPR012677, Nucleotide-bd_a/b_plait_sf IPR035979, RBD_domain_sf IPR000504, RRM_dom IPR036388, WH-like_DNA-bd_sf IPR036390, WH_DNA-bd_sf |
Pfami | View protein in Pfam PF05383, La, 1 hit PF00076, RRM_1, 1 hit PF08777, RRM_3, 1 hit |
PRINTSi | PR00302, LUPUSLA |
SMARTi | View protein in SMART SM00715, LA, 1 hit SM00360, RRM, 1 hit |
SUPFAMi | SSF46785, SSF46785, 1 hit SSF54928, SSF54928, 2 hits |
PROSITEi | View protein in PROSITE PS50961, HTH_LA, 1 hit PS50102, RRM, 1 hit PS51939, XRRM, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LARP7_RAT | |
Accessioni | Q5XI01Primary (citable) accession number: Q5XI01 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 20, 2007 |
Last sequence update: | March 20, 2007 | |
Last modified: | April 7, 2021 | |
This is version 115 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families