UniProtKB - Q5XGB9 (AMPL_XENTR)
Cytosol aminopeptidase
lap3
Functioni
Cytosolic metallopeptidase that catalyzes the removal of unsubstituted N-terminal hydrophobic amino acids from various peptides. The presence of Zn2+ ions is essential for the peptidase activity, and the association with other cofactors can modulate the substrate spectificity of the enzyme. For instance, in the presence of Mn2+, it displays a specific Cys-Gly hydrolyzing activity of Cys-Gly-S-conjugates. Involved in the metabolism of glutathione and in the degradation of glutathione S-conjugates, which may play a role in the control of the cell redox status.
By similarityCatalytic activityi
- EC:3.4.13.23By similarityThis reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
- Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.By similarity EC:3.4.11.1
- Release of N-terminal proline from a peptide.By similarity EC:3.4.11.5
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 200 | Zinc 3; via carbonyl oxygen; structuralBy similarity | 1 | |
Metal bindingi | 201 | Zinc 3; via carbonyl oxygen; structuralBy similarity | 1 | |
Metal bindingi | 280 | Zinc 2; catalyticBy similarity | 1 | |
Binding sitei | 280 | SubstrateBy similarity | 1 | |
Metal bindingi | 285 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 285 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 285 | Zinc 2; catalyticBy similarity | 1 | |
Binding sitei | 285 | SubstrateBy similarity | 1 | |
Binding sitei | 290 | SubstrateBy similarity | 1 | |
Active sitei | 292 | By similarity | 1 | |
Binding sitei | 292 | SubstrateBy similarity | 1 | |
Metal bindingi | 301 | Zinc 3; via carbonyl oxygen; structuralBy similarity | 1 | |
Metal bindingi | 303 | Zinc 2; catalyticBy similarity | 1 | |
Binding sitei | 303 | SubstrateBy similarity | 1 | |
Metal bindingi | 362 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 362 | Zinc 1; catalyticBy similarity | 1 | |
Binding sitei | 362 | SubstrateBy similarity | 1 | |
Metal bindingi | 364 | Magnesium; catalyticBy similarity | 1 | |
Metal bindingi | 364 | Zinc 1; catalyticBy similarity | 1 | |
Metal bindingi | 364 | Zinc 2; catalyticBy similarity | 1 | |
Active sitei | 366 | By similarity | 1 |
GO - Molecular functioni
- carboxypeptidase activity Source: RHEA
- manganese ion binding Source: InterPro
- metalloaminopeptidase activity Source: InterPro
- peptidase activity Source: GO_Central
Keywordsi
Molecular function | Aminopeptidase, Hydrolase, Protease |
Ligand | Magnesium, Manganese, Metal-binding, Zinc |
Protein family/group databases
MEROPSi | M17.001 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:lap3 |
Organismi | Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) |
Taxonomic identifieri | 8364 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana |
Proteomesi |
|
Organism-specific databases
Xenbasei | XB-GENE-1006711, lap3 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: GO_Central
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000274147 | 1 – 520 | Cytosol aminopeptidaseAdd BLAST | 520 |
Proteomic databases
PaxDbi | Q5XGB9 |
Interactioni
Subunit structurei
Homohexamer.
By similarityFamily & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG2597, Eukaryota |
GeneTreei | ENSGT00530000063255 |
HOGENOMi | CLU_013734_1_2_1 |
InParanoidi | Q5XGB9 |
OrthoDBi | 562530at2759 |
PhylomeDBi | Q5XGB9 |
Family and domain databases
CDDi | cd00433, Peptidase_M17, 1 hit |
Gene3Di | 3.40.220.10, 1 hit |
HAMAPi | MF_00181, Cytosol_peptidase_M17, 1 hit |
InterProi | View protein in InterPro IPR011356, Leucine_aapep/pepB IPR043472, Macro_dom-like IPR000819, Peptidase_M17_C IPR023042, Peptidase_M17_leu_NH2_pept IPR008283, Peptidase_M17_N |
PANTHERi | PTHR11963, PTHR11963, 1 hit |
Pfami | View protein in Pfam PF00883, Peptidase_M17, 1 hit PF02789, Peptidase_M17_N, 1 hit |
PRINTSi | PR00481, LAMNOPPTDASE |
SUPFAMi | SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS00631, CYTOSOL_AP, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MLPFRTLLKW SVNRNCCRGF AVSQQNYNSV KKGLVLGVYE KEKEEESLTL
60 70 80 90 100
TNAGDAVDNA VLGKLRDQLA RSGPSLKKGK SRIFYGLHED FPSIVVVGLG
110 120 130 140 150
KKSAGVNQHE LWNEAKENIR AAVSVGCRQM QDMEIVQVEV DPCGDAQAAA
160 170 180 190 200
EGAVLGLFEY NEMKKKKKKA VTTHLHGSSE ITAWEKGVLY AEGQNLARHL
210 220 230 240 250
MEAPANYITP TKFAETFEQR LANMGSNVKV FTRSKQWIEE QQMGAFLSVA
260 270 280 290 300
KGSEEPPVFL EIHYSGSSDA SQPPLVFVGK GVTFDSGGIS LKPSSGMDAM
310 320 330 340 350
RGDMGGAATV CSAITTAAKL KLPINIISLA PLCENMPNGR ANKPGDVVKA
360 370 380 390 400
KNGKTIQVDN TDAEGRLLLA DALCYAHSFN PRAIVNAATL TGAMDVALGS
410 420 430 440 450
AAAGVFTNSS WLWTHLQEAS VVTGDRVWRM PLFEHYSKQV TESALADLNN
460 470 480 490 500
IGKYSRSGGA CTAAAFLKEF VTAPHWAHLD IAGVMSNKDE VPYLRKGMSG
510 520
RPTRTLIEFA ARLSEDKQTI
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketG1K3J6 | G1K3J6_XENTR | Cysteinylglycine-S-conjugate dipept... | ttc22 lap3 | 521 | Annotation score: | ||
A0A6I8R114 | A0A6I8R114_XENTR | Cysteinylglycine-S-conjugate dipept... | lap3 | 496 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC084523 mRNA Translation: AAH84523.1 |
RefSeqi | NP_001011124.1, NM_001011124.2 |
Genome annotation databases
Ensembli | ENSXETT00000043882; ENSXETP00000043882; ENSXETG00000020343 |
GeneIDi | 496537 |
KEGGi | xtr:496537 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC084523 mRNA Translation: AAH84523.1 |
RefSeqi | NP_001011124.1, NM_001011124.2 |
3D structure databases
SMRi | Q5XGB9 |
ModBasei | Search... |
Protein family/group databases
MEROPSi | M17.001 |
Proteomic databases
PaxDbi | Q5XGB9 |
Genome annotation databases
Ensembli | ENSXETT00000043882; ENSXETP00000043882; ENSXETG00000020343 |
GeneIDi | 496537 |
KEGGi | xtr:496537 |
Organism-specific databases
CTDi | 51056 |
Xenbasei | XB-GENE-1006711, lap3 |
Phylogenomic databases
eggNOGi | KOG2597, Eukaryota |
GeneTreei | ENSGT00530000063255 |
HOGENOMi | CLU_013734_1_2_1 |
InParanoidi | Q5XGB9 |
OrthoDBi | 562530at2759 |
PhylomeDBi | Q5XGB9 |
Family and domain databases
CDDi | cd00433, Peptidase_M17, 1 hit |
Gene3Di | 3.40.220.10, 1 hit |
HAMAPi | MF_00181, Cytosol_peptidase_M17, 1 hit |
InterProi | View protein in InterPro IPR011356, Leucine_aapep/pepB IPR043472, Macro_dom-like IPR000819, Peptidase_M17_C IPR023042, Peptidase_M17_leu_NH2_pept IPR008283, Peptidase_M17_N |
PANTHERi | PTHR11963, PTHR11963, 1 hit |
Pfami | View protein in Pfam PF00883, Peptidase_M17, 1 hit PF02789, Peptidase_M17_N, 1 hit |
PRINTSi | PR00481, LAMNOPPTDASE |
SUPFAMi | SSF52949, SSF52949, 1 hit |
PROSITEi | View protein in PROSITE PS00631, CYTOSOL_AP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AMPL_XENTR | |
Accessioni | Q5XGB9Primary (citable) accession number: Q5XGB9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2007 |
Last sequence update: | November 23, 2004 | |
Last modified: | February 23, 2022 | |
This is version 96 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - SIMILARITY comments
Index of protein domains and families