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Protein

Non-canonical poly(A) RNA polymerase PAPD7

Gene

TENT4A

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation.1 Publication

Caution

Was originally thought to have DNA polymerase activity.Curated

Catalytic activityi

ATP + RNA(n) = diphosphate + RNA(n+1).

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi277Magnesium or manganese; catalyticBy similarity1
Metal bindingi279Magnesium or manganese; catalyticBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • polynucleotide adenylyltransferase activity Source: UniProtKB-EC
  • SMC family protein binding Source: UniProtKB

GO - Biological processi

  • double-strand break repair Source: UniProtKB
  • mitotic chromosome condensation Source: UniProtKB
  • mRNA processing Source: UniProtKB-KW
  • response to drug Source: UniProtKB
  • sister chromatid cohesion Source: UniProtKB

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processmRNA processing
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6802952 Signaling by BRAF and RAF fusions

Names & Taxonomyi

Protein namesi
Recommended name:
Non-canonical poly(A) RNA polymerase PAPD7Curated (EC:2.7.7.19)
Alternative name(s):
DNA polymerase sigma
LAK-1
PAP-associated domain-containing protein 7
TRAMP-like complex polyadenylate polymerase
Terminal nucleotidyltransferase 4AImported
Terminal uridylyltransferase 5
Short name:
TUTase 5
Topoisomerase-related function protein 4-1
Short name:
TRF4-1
Gene namesi
Name:TENT4AImported
Synonyms:PAPD7Imported, POLSImported, TRF4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000112941.13
HGNCiHGNC:16705 TENT4A
MIMi605198 gene
neXtProtiNX_Q5XG87

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi167 – 230Missing : Localizes to cytoplasm. 1 PublicationAdd BLAST64

Organism-specific databases

DisGeNETi11044
OpenTargetsiENSG00000112941
PharmGKBiPA33523

Polymorphism and mutation databases

BioMutaiPAPD7
DMDMi60392922

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203081 – 772Non-canonical poly(A) RNA polymerase PAPD7Add BLAST772

Proteomic databases

EPDiQ5XG87
PaxDbiQ5XG87
PeptideAtlasiQ5XG87
PRIDEiQ5XG87
ProteomicsDBi65815

PTM databases

iPTMnetiQ5XG87
PhosphoSitePlusiQ5XG87

Expressioni

Gene expression databases

BgeeiENSG00000112941
CleanExiHS_POLS
ExpressionAtlasiQ5XG87 baseline and differential
GenevisibleiQ5XG87 HS

Organism-specific databases

HPAiHPA045487
HPA046742

Interactioni

Subunit structurei

Component of a nuclear TRAMP-like complex, an ATP-dependent exosome regulatory complex consisting of a helicase (MTREX), an oligadenylate polymerase (TENT4B or TENT4A), and a substrate specific RNA-binding factor (ZCCHC7 or ZCCHC8). Several TRAMP-like complexes exist with specific compositions and are associated with nuclear, or nucleolar RNA exosomes.

GO - Molecular functioni

  • SMC family protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi116232, 5 interactors
IntActiQ5XG87, 1 interactor
MINTiQ5XG87
STRINGi9606.ENSP00000230859

Structurei

3D structure databases

ProteinModelPortaliQ5XG87
SMRiQ5XG87
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini408 – 468PAP-associatedAdd BLAST61

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi27 – 79Ala-richAdd BLAST53
Compositional biasi85 – 117Ser-richAdd BLAST33
Compositional biasi576 – 602Ser-richAdd BLAST27

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated

Phylogenomic databases

eggNOGiKOG1906 Eukaryota
COG5260 LUCA
GeneTreeiENSGT00400000022055
HOGENOMiHOG000290646
HOVERGENiHBG053636
InParanoidiQ5XG87
KOiK03514
OrthoDBiEOG091G05VI
TreeFamiTF313939

Family and domain databases

InterProiView protein in InterPro
IPR002058 PAP_assoc
IPR002934 Polymerase_NTP_transf_dom
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF03828 PAP_assoc, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5XG87-1) [UniParc]FASTAAdd to basket
Also known as: l

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQIWETSQGV GRGGSGFASY FCLNSPALDT AAAAGAAGRG SGGLGPALPA
60 70 80 90 100
ASPPPPGPTA PAALPPALLT ALGPAAEGAR RLHKSPSLSS SSSSSSSNAE
110 120 130 140 150
SGTESPGCSS SSSSSASLGR PGGGRGGAFF NFADGAPSAP GTANGHPGPR
160 170 180 190 200
GPAPAGSPSQ HQFHPGRRKR ENKASTYGLN YLLSGSRAAA LSGGGGPGAQ
210 220 230 240 250
APRPGTPWKS RAYSPGIQGL HEEIIDFYNF MSPCPEEAAM RREVVKRIET
260 270 280 290 300
VVKDLWPTAD VQIFGSFSTG LYLPTSDIDL VVFGKWERPP LQLLEQALRK
310 320 330 340 350
HNVAEPCSIK VLDKATVPII KLTDQETEVK VDISFNMETG VRAAEFIKNY
360 370 380 390 400
MKKYSLLPYL ILVLKQFLLQ RDLNEVFTGG ISSYSLILMA ISFLQLHPRI
410 420 430 440 450
DARRADENLG MLLVEFFELY GRNFNYLKTG IRIKEGGAYI AKEEIMKAMT
460 470 480 490 500
SGYRPSMLCI EDPLLPGNDV GRSSYGAMQV KQVFDYAYIV LSHAVSPLAR
510 520 530 540 550
SYPNRDAEST LGRIIKVTQE VIDYRRWIKE KWGSKAHPSP GMDSRIKIKE
560 570 580 590 600
RIATCNGEQT QNREPESPYG QRLTLSLSSP QLLSSGSSAS SVSSLSGSDV
610 620 630 640 650
DSDTPPCTTP SVYQFSLQAP APLMAGLPTA LPMPSGKPQP TTSRTLIMTT
660 670 680 690 700
NNQTRFTIPP PTLGVAPVPC RQAGVEGTAS LKAVHHMSSP AIPSASPNPL
710 720 730 740 750
SSPHLYHKQH NGMKLSMKGS HGHTQGGGYS SVGSGGVRPP VGNRGHHQYN
760 770
RTGWRRKKHT HTRDSLPVSL SR
Length:772
Mass (Da):82,360
Last modified:February 19, 2014 - v3
Checksum:iA5EBEC15CA37689C
GO
Isoform 2 (identifier: Q5XG87-2) [UniParc]FASTAAdd to basket
Also known as: s

The sequence of this isoform differs from the canonical sequence as follows:
     1-230: Missing.

Note: Exhibits poor nucleotidyl transferase activity.
Show »
Length:542
Mass (Da):59,874
Checksum:i233DA7E2222C2FB0
GO

Sequence cautioni

The sequence AAD45198 differs from that shown. Reason: Frameshift at position 474.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_021175556N → S1 PublicationCorresponds to variant dbSNP:rs28381415Ensembl.1
Natural variantiVAR_021176626G → S1 PublicationCorresponds to variant dbSNP:rs28381418Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0537321 – 230Missing in isoform 2. 4 PublicationsAdd BLAST230

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF089896 mRNA Translation: AAD45198.1 Sequence problems.
KC424495 mRNA Translation: AGE92663.1
AB005754 mRNA Translation: BAA24434.2
AY623114 Genomic DNA Translation: AAT38110.1
AK289857 mRNA Translation: BAF82546.1
CH471102 Genomic DNA Translation: EAX08101.1
BC084567 mRNA Translation: AAH84567.2
BC117137 mRNA Translation: AAI17138.1
BC126106 mRNA Translation: AAI26107.1
CCDSiCCDS3871.1 [Q5XG87-2]
RefSeqiNP_001165276.1, NM_001171805.1
NP_001165277.1, NM_001171806.1
NP_008930.1, NM_006999.4 [Q5XG87-2]
UniGeneiHs.481542

Genome annotation databases

EnsembliENST00000631941; ENSP00000488642; ENSG00000112941 [Q5XG87-2]
GeneIDi11044
KEGGihsa:11044
UCSCiuc003jdx.1 human [Q5XG87-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPAPD7_HUMAN
AccessioniPrimary (citable) accession number: Q5XG87
Secondary accession number(s): A8K1E2
, M1JCE6, O43289, Q17RZ1, Q9Y6C1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 19, 2014
Last modified: July 18, 2018
This is version 126 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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