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Protein

Thymidylate synthase

Gene

thyA

Organism
Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154NucleophileUniRule annotation1
Binding sitei1815,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei189dUMPUniRule annotation1
Binding sitei2785,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi133 – 134dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi178 – 181dUMPUniRule annotation4
Nucleotide bindingi219 – 221dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

UniPathwayi
UPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:M6_Spy0705
OrganismiStreptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394)
Taxonomic identifieri286636 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
Proteomesi
  • UP000001167 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410341 – 279Thymidylate synthaseAdd BLAST279

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliQ5XCM3
SMRiQ5XCM3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000257898
KOiK00560

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit

Sequencei

Sequence statusi: Complete.

Q5XCM3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTKADQIFKA NIQKIINEGS LSEQARPKYK DGRTAHSKYI TGAFAEYDLA
60 70 80 90 100
KGEFPITTLR PIPIKSAIKE LFWIYQDQSN SLDVLEAKYN VHYWNEWEVD
110 120 130 140 150
QTRTIGQRYG AVVKKHDIIS KILKQLAENP WNRRNVISLW DYEAFEETKG
160 170 180 190 200
LLPCAFQIMF DVRRVGEDLY LDASLTQRSN DILVAHHINA MQYVALQMMI
210 220 230 240 250
AKHFGWKIGK FFYFVNNLHI YDNQFDQAQE LLKRQPVASQ PKLVLNVPDG
260 270
TNFFDIKPDD FELQNYDPVK PQLHFDLAI
Length:279
Mass (Da):32,564
Last modified:April 26, 2005 - v2
Checksum:i6D8B39FA881D4B8D
GO

Sequence cautioni

The sequence AAT86840 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA Translation: AAT86840.1 Different initiation.
RefSeqiWP_011017679.1, NC_006086.1

Genome annotation databases

EnsemblBacteriaiAAT86840; AAT86840; M6_Spy0705
KEGGispa:M6_Spy0705

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000003 Genomic DNA Translation: AAT86840.1 Different initiation.
RefSeqiWP_011017679.1, NC_006086.1

3D structure databases

ProteinModelPortaliQ5XCM3
SMRiQ5XCM3
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAT86840; AAT86840; M6_Spy0705
KEGGispa:M6_Spy0705

Phylogenomic databases

HOGENOMiHOG000257898
KOiK00560

Enzyme and pathway databases

UniPathwayi
UPA00575

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
IPR020940 Thymidylate_synthase_AS
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 1 hit
PROSITEiView protein in PROSITE
PS00091 THYMIDYLATE_SYNTHASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTYSY_STRP6
AccessioniPrimary (citable) accession number: Q5XCM3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: October 10, 2018
This is version 87 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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