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Protein

F-actin-uncapping protein LRRC16A

Gene

CARMIL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cell membrane-cytoskeleton-associated protein that plays a role in the regulation of actin polymerization at the barbed end of actin filaments. Prevents F-actin heterodimeric capping protein (CP) activity at the leading edges of migrating cells, and hence generates uncapped barbed ends and enhances actin polymerization, however, seems unable to nucleate filaments (PubMed:16054028). Plays a role in lamellipodial protrusion formations and cell migration (PubMed:19846667).2 Publications

GO - Molecular functioni

  • protein-containing complex binding Source: UniProtKB

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-HSA-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
F-actin-uncapping protein LRRC16ACurated
Alternative name(s):
CARMIL homologBy similarity
Capping protein regulator and myosin 1 linker protein 1Imported
Capping protein, Arp2/3 and myosin-I linker homolog 1By similarity
Capping protein, Arp2/3 and myosin-I linker protein 11 Publication
Leucine-rich repeat-containing protein 16AImported
Gene namesi
Name:CARMIL11 PublicationImported
Synonyms:CARMILBy similarity, LRRC16, LRRC16A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000079691.17
HGNCiHGNC:21581 CARMIL1
MIMi609593 gene
neXtProtiNX_Q5VZK9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi55604
OpenTargetsiENSG00000079691
PharmGKBiPA162394368

Polymorphism and mutation databases

BioMutaiLRRC16A
DMDMi74762279

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003258151 – 1371F-actin-uncapping protein LRRC16AAdd BLAST1371

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei122PhosphoserineCombined sources1
Modified residuei916PhosphothreonineCombined sources1
Modified residuei968PhosphoserineCombined sources1
Modified residuei1067PhosphoserineCombined sources1
Modified residuei1094PhosphoserineCombined sources1
Modified residuei1228PhosphothreonineCombined sources1
Modified residuei1280PhosphoserineCombined sources1
Modified residuei1288PhosphoserineCombined sources1
Modified residuei1291PhosphoserineCombined sources1
Modified residuei1315PhosphoserineCombined sources1
Modified residuei1324PhosphoserineCombined sources1
Modified residuei1331PhosphoserineCombined sources1
Modified residuei1360PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5VZK9
MaxQBiQ5VZK9
PaxDbiQ5VZK9
PeptideAtlasiQ5VZK9
PRIDEiQ5VZK9
ProteomicsDBi65702
65703 [Q5VZK9-2]
65704 [Q5VZK9-3]
65705 [Q5VZK9-4]

PTM databases

iPTMnetiQ5VZK9
PhosphoSitePlusiQ5VZK9

Expressioni

Tissue specificityi

Expressed in lung, placenta, small intestine, liver, thymus, colon, skeletal muscle, heart and brain. Higher expression in kidney.1 Publication

Gene expression databases

BgeeiENSG00000079691 Expressed in 175 organ(s), highest expression level in oocyte
CleanExiHS_LRRC16A
ExpressionAtlasiQ5VZK9 baseline and differential
GenevisibleiQ5VZK9 HS

Organism-specific databases

HPAiHPA029038
HPA029039

Interactioni

Subunit structurei

Homodimer (PubMed:19846667). Interacts (via C-terminus) with heterodimer capping protein (CP); this interaction uncaps barbed ends capped by CP, enhances barbed-end actin polymerization and promotes lamellipodial formation and cell migration (By similarity). Interacts with heterodimer capping protein (CP) (PubMed:19846667). Interacts with MYO1E (PubMed:19846667). Interacts with TRIO (PubMed:19846667).By similarity1 Publication

Protein-protein interaction databases

BioGridi120745, 18 interactors
DIPiDIP-56920N
IntActiQ5VZK9, 7 interactors
STRINGi9606.ENSP00000331983

Structurei

Secondary structure

11371
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5VZK9
SMRiQ5VZK9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5VZK9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati245 – 269LRR 1Add BLAST25
Repeati275 – 298LRR 2Add BLAST24
Repeati304 – 327LRR 3Add BLAST24
Repeati336 – 363LRR 4Add BLAST28
Repeati391 – 418LRR 5Add BLAST28
Repeati423 – 447LRR 6Add BLAST25
Repeati481 – 506LRR 7Add BLAST26
Repeati543 – 566LRR 8Add BLAST24
Repeati570 – 593LRR 9Add BLAST24
Repeati654 – 678LRR 10Add BLAST25
Repeati958 – 981LRR 11Add BLAST24

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni958 – 1082Inhibits capping activity of CAPZA2By similarityAdd BLAST125
Regioni1055 – 1089Necessary for localization at the cell membrane1 PublicationAdd BLAST35

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili710 – 734Sequence analysisAdd BLAST25

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1243 – 1250Poly-Ser8

Domaini

The C-terminus is necessary for localization to the cell membrane (PubMed:26578515).1 Publication

Sequence similaritiesi

Belongs to the CARMIL family.Curated

Keywords - Domaini

Coiled coil, Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG4242 Eukaryota
ENOG410Y5JZ LUCA
GeneTreeiENSGT00390000014487
HOGENOMiHOG000230565
HOVERGENiHBG108094
InParanoidiQ5VZK9
KOiK20493
OMAiGVQMMGS
OrthoDBiEOG091G01FI
PhylomeDBiQ5VZK9
TreeFamiTF316381

Family and domain databases

Gene3Di2.30.29.30, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR029764 CARMIL1
IPR031943 CARMIL_C
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
IPR011993 PH-like_dom_sf
PANTHERiPTHR24112:SF39 PTHR24112:SF39, 1 hit
PfamiView protein in Pfam
PF16000 CARMIL_C, 1 hit
PF13516 LRR_6, 2 hits

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5VZK9-1) [UniParc]FASTAAdd to basket
Also known as: CARMIL1a1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTEESSDVPR ELIESIKDVI GRKIKISVKK KVKLEVKGDK VENKVLVLTS
60 70 80 90 100
CRAFLVTARI PTKLELTFSY LEIHGVVCSK SAQMIVETEK CSISMKMASP
110 120 130 140 150
EDVSEVLAHI GTCLRKIFPG LSPVRIMKKV SMEPSERLAS LQALWDSQTV
160 170 180 190 200
AEQGPCGGFS QMYACVCDWL GFSYREEVQW DVDTIYLTQD TRELNLQDFS
210 220 230 240 250
HLDHRDLIPI IAALEYNQWF TKLSSKDLKL STDVCEQILR VVSRSNRLEE
260 270 280 290 300
LVLENAGLRT DFAQKLASAL AHNPNSGLHT INLAGNPLED RGVSSLSIQF
310 320 330 340 350
AKLPKGLKHL NLSKTSLSPK GVNSLSQSLS ANPLTASTLV HLDLSGNVLR
360 370 380 390 400
GDDLSHMYNF LAQPNAIVHL DLSNTECSLD MVCGALLRGC LQYLAVLNLS
410 420 430 440 450
RTVFSHRKGK EVPPSFKQFF SSSLALMHIN LSGTKLSPEP LKALLLGLAC
460 470 480 490 500
NHNLKGVSLD LSNCELRSGG AQVLEGCIAE IHNITSLDIS DNGLESDLST
510 520 530 540 550
LIVWLSKNRS IQHLALGKNF NNMKSKNLTP VLDNLVQMIQ DEESPLQSLS
560 570 580 590 600
LADSKLKTEV TIIINALGSN TSLTKVDISG NGMGDMGAKM LAKALQINTK
610 620 630 640 650
LRTVIWDKNN ITAQGFQDIA VAMEKNYTLR FMPIPMYDAS QALKTNPEKT
660 670 680 690 700
EDALQKIENY LLRNHETRKY LQEQAYRLQQ GIVTSTTQQM IDRICVKVQD
710 720 730 740 750
HLNSLRNCGG DAIQEDLKSA ERLMRDAKNS KTLLPNLYHV GGASWAGASG
760 770 780 790 800
LLSSPIQETL ESMAGEVTRV VDEQLKALLE SMVDAAENLC PNVMKKAHIR
810 820 830 840 850
QDLIHASTEK ISIPRTFVKN VLLEQSGIDI LNKISEVKLT VASFLSDRIV
860 870 880 890 900
DEILDALSHC HHKLADHFSR RGKTLPQQES LEIELAEEKP VKRSIITVEE
910 920 930 940 950
LTEIERLEDL DTCMMTPKSK RKSIHSRMLR PVSRAFEMEF DLDKALEEVP
960 970 980 990 1000
IHIEDPPFPS LRQEKRSSGF ISELPSEEGK KLEHFTKLRP KRNKKQQPTQ
1010 1020 1030 1040 1050
AAVCAANIVS QDGEQNGLMG RVDEGVDEFF TKKVTKMDSK KWSTRGSESH
1060 1070 1080 1090 1100
ELNEGGDEKK KRDSRKSSGF LNLIKSRSKS ERPPTILMTE EPSSPKGAVR
1110 1120 1130 1140 1150
SPPVDCPRKD TKAAEHNGNS ERIEEIKTPD SFEESQGEEI GKVERSDSKS
1160 1170 1180 1190 1200
SPQAGRRYGV QVMGSGLLAE MKAKQEKRAA CAQKKLGNDA VSQDSSSPAL
1210 1220 1230 1240 1250
SGVERSDGGG AVPKLHPGLP ENRFGLGTPE KNTKAEPKAE AGSRSRSSSS
1260 1270 1280 1290 1300
TPTSPKPLLQ SPKPSLAARP VIPQKPRTAS RPDDIPDSPS SPKVALLPPV
1310 1320 1330 1340 1350
LKKVPSDKER DGQSSPQPSP RTFSQEVSRR SWGQQAQEYQ EQKQRSSSKD
1360 1370
GHQGSKSNDS GEEAEKEFIF V
Length:1,371
Mass (Da):151,557
Last modified:December 7, 2004 - v1
Checksum:i6DECD6387FD73DCE
GO
Isoform 2 (identifier: Q5VZK9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1177-1221: Missing.

Note: No experimental confirmation available.
Show »
Length:1,326
Mass (Da):147,088
Checksum:iBC4881025483CA27
GO
Isoform 3 (identifier: Q5VZK9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     486-499: SLDISDNGLESDLS → QLGTRYRNAVLRVY
     500-1371: Missing.

Note: No experimental confirmation available.
Show »
Length:499
Mass (Da):55,117
Checksum:iA77D54FABEE374C8
GO
Isoform 4 (identifier: Q5VZK9-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-161: Missing.
     292-337: GVSSLSIQFA...SLSANPLTAS → ETTTKIKRQN...NYYSDFSHDG
     338-1371: Missing.

Note: No experimental confirmation available.
Show »
Length:176
Mass (Da):20,181
Checksum:i806D8D31AAC2A04A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0U1RQQ1A0A0U1RQQ1_HUMAN
F-actin-uncapping protein LRRC16A
CARMIL1
932Annotation score:
A0A0U1RR91A0A0U1RR91_HUMAN
F-actin-uncapping protein LRRC16A
CARMIL1
196Annotation score:

Sequence cautioni

The sequence BAA90912 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255N → D in BAC85701 (PubMed:14702039).Curated1
Sequence conflicti889K → R in BAA90912 (PubMed:14702039).Curated1
Sequence conflicti1202G → S in ACI49709 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_03992377V → I. Corresponds to variant dbSNP:rs9358856Ensembl.1
Natural variantiVAR_039924545P → L. Corresponds to variant dbSNP:rs12207840Ensembl.1
Natural variantiVAR_039925639A → G. Corresponds to variant dbSNP:rs7454756Ensembl.1
Natural variantiVAR_0399261117N → S. Corresponds to variant dbSNP:rs9885914Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0324151 – 161Missing in isoform 4. 1 PublicationAdd BLAST161
Alternative sequenceiVSP_032416292 – 337GVSSL…PLTAS → ETTTKIKRQNVPTVLQTYLV VCPSDYQPCPLPLGKDNYYS DFSHDG in isoform 4. 1 PublicationAdd BLAST46
Alternative sequenceiVSP_032417338 – 1371Missing in isoform 4. 1 PublicationAdd BLAST1034
Alternative sequenceiVSP_032418486 – 499SLDIS…ESDLS → QLGTRYRNAVLRVY in isoform 3. 1 PublicationAdd BLAST14
Alternative sequenceiVSP_032419500 – 1371Missing in isoform 3. 1 PublicationAdd BLAST872
Alternative sequenceiVSP_0324201177 – 1221Missing in isoform 2. 1 PublicationAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ009082 mRNA Translation: ACI49709.1
AK123817 mRNA Translation: BAC85701.1
AK125696 mRNA Translation: BAC86250.1
AK000055 mRNA Translation: BAA90912.1 Different initiation.
AL022170 Genomic DNA No translation available.
AL024509 Genomic DNA No translation available.
AL160037 Genomic DNA No translation available.
CCDSiCCDS54973.1 [Q5VZK9-1]
RefSeqiNP_060110.4, NM_017640.5 [Q5VZK9-1]
UniGeneiHs.649550

Genome annotation databases

EnsembliENST00000329474; ENSP00000331983; ENSG00000079691 [Q5VZK9-1]
GeneIDi55604
KEGGihsa:55604
UCSCiuc011djw.2 human [Q5VZK9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ009082 mRNA Translation: ACI49709.1
AK123817 mRNA Translation: BAC85701.1
AK125696 mRNA Translation: BAC86250.1
AK000055 mRNA Translation: BAA90912.1 Different initiation.
AL022170 Genomic DNA No translation available.
AL024509 Genomic DNA No translation available.
AL160037 Genomic DNA No translation available.
CCDSiCCDS54973.1 [Q5VZK9-1]
RefSeqiNP_060110.4, NM_017640.5 [Q5VZK9-1]
UniGeneiHs.649550

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3LK2X-ray2.20T961-1012[»]
3LK3X-ray2.68T964-1078[»]
ProteinModelPortaliQ5VZK9
SMRiQ5VZK9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120745, 18 interactors
DIPiDIP-56920N
IntActiQ5VZK9, 7 interactors
STRINGi9606.ENSP00000331983

PTM databases

iPTMnetiQ5VZK9
PhosphoSitePlusiQ5VZK9

Polymorphism and mutation databases

BioMutaiLRRC16A
DMDMi74762279

Proteomic databases

EPDiQ5VZK9
MaxQBiQ5VZK9
PaxDbiQ5VZK9
PeptideAtlasiQ5VZK9
PRIDEiQ5VZK9
ProteomicsDBi65702
65703 [Q5VZK9-2]
65704 [Q5VZK9-3]
65705 [Q5VZK9-4]

Protocols and materials databases

DNASUi55604
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000329474; ENSP00000331983; ENSG00000079691 [Q5VZK9-1]
GeneIDi55604
KEGGihsa:55604
UCSCiuc011djw.2 human [Q5VZK9-1]

Organism-specific databases

CTDi55604
DisGeNETi55604
EuPathDBiHostDB:ENSG00000079691.17
GeneCardsiCARMIL1
H-InvDBiHIX0005632
HGNCiHGNC:21581 CARMIL1
HPAiHPA029038
HPA029039
MIMi609593 gene
neXtProtiNX_Q5VZK9
OpenTargetsiENSG00000079691
PharmGKBiPA162394368
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4242 Eukaryota
ENOG410Y5JZ LUCA
GeneTreeiENSGT00390000014487
HOGENOMiHOG000230565
HOVERGENiHBG108094
InParanoidiQ5VZK9
KOiK20493
OMAiGVQMMGS
OrthoDBiEOG091G01FI
PhylomeDBiQ5VZK9
TreeFamiTF316381

Enzyme and pathway databases

ReactomeiR-HSA-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

ChiTaRSiCARMIL1 human
EvolutionaryTraceiQ5VZK9
GeneWikiiLRRC16A
GenomeRNAii55604
PROiPR:Q5VZK9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000079691 Expressed in 175 organ(s), highest expression level in oocyte
CleanExiHS_LRRC16A
ExpressionAtlasiQ5VZK9 baseline and differential
GenevisibleiQ5VZK9 HS

Family and domain databases

Gene3Di2.30.29.30, 1 hit
3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR029764 CARMIL1
IPR031943 CARMIL_C
IPR001611 Leu-rich_rpt
IPR032675 LRR_dom_sf
IPR011993 PH-like_dom_sf
PANTHERiPTHR24112:SF39 PTHR24112:SF39, 1 hit
PfamiView protein in Pfam
PF16000 CARMIL_C, 1 hit
PF13516 LRR_6, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCARL1_HUMAN
AccessioniPrimary (citable) accession number: Q5VZK9
Secondary accession number(s): B8X1J0
, Q6ZUH5, Q6ZW07, Q9NXU7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: December 7, 2004
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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