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Protein

Terminal uridylyltransferase 7

Gene

TUT7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Uridylyltransferase that mediates the terminal uridylation of mRNAs with short (less than 25 nucleotides) poly(A) tails, hence facilitating global mRNA decay (PubMed:19703396, PubMed:25480299). Essential for both oocyte maturation and fertility. Through 3' terminal uridylation of mRNA, sculpts, with TUT7, the maternal transcriptome by eliminating transcripts during oocyte growth (By similarity). Involved in microRNA (miRNA)-induced gene silencing through uridylation of deadenylated miRNA targets (PubMed:25480299). Also functions as an integral regulator of microRNA biogenesiS using 3 different uridylation mechanisms (PubMed:25979828). Acts as a suppressor of miRNA biogenesis by mediating the terminal uridylation of some miRNA precursors, including that of let-7 (pre-let-7). Uridylated pre-let-7 RNA is not processed by Dicer and undergo degradation. Pre-let-7 uridylation is strongly enhanced in the presence of LIN28A (PubMed:22898984). In the absence of LIN28A, TUT7 and TUT4 monouridylate group II pre-miRNAs, which includes most of pre-let7 members, that shapes an optimal 3' end overhang for efficient processing (PubMed:25979828, PubMed:28671666). Add oligo-U tails to truncated pre-miRNAS with a 5' overhang which may promote rapid degradation of non-fnctional pre-miRNA species (PubMed:25979828). Does not play a role in replication-dependent histone mRNA degradation (PubMed:18172165). Due to functional redundancy between ZCCHC6 and ZCCHC11, the identification of the specific role of each of these proteins is difficult (PubMed:25979828, PubMed:25480299, PubMed:19703396, PubMed:22898984, PubMed:18172165, PubMed:28671666).By similarity6 Publications

Catalytic activityi

UTP + RNA(n) = diphosphate + RNA(n+1).1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi1058Magnesium or manganese; catalyticBy similarity1
Metal bindingi1060Magnesium or manganese; catalyticBy similarity1
Binding sitei1130UTPCombined sources1
Binding sitei1152UTPCombined sources1
Binding sitei1286UTPCombined sources1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri244 – 274Matrin-typePROSITE-ProRule annotationAdd BLAST31
Zinc fingeri963 – 980CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri1345 – 1362CCHC-type 2PROSITE-ProRule annotationCombined sources1 PublicationAdd BLAST18
Zinc fingeri1451 – 1468CCHC-type 3PROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
LigandMagnesium, Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.7.52 2681
ReactomeiR-HSA-429947 Deadenylation of mRNA

Names & Taxonomyi

Protein namesi
Recommended name:
Terminal uridylyltransferase 7Curated (EC:2.7.7.521 Publication)
Short name:
TUTase 7Curated
Alternative name(s):
Zinc finger CCHC domain-containing protein 6
Gene namesi
Name:TUT7Imported
Synonyms:HS2, KIAA1711, ZCCHC6Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000083223.17
HGNCiHGNC:25817 TUT7
MIMi613467 gene
neXtProtiNX_Q5VYS8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1097 – 1099LPI → WPW: Abolishes monouridylation activity. 1 Publication3

Organism-specific databases

OpenTargetsiENSG00000083223
PharmGKBiPA134971144

Polymorphism and mutation databases

BioMutaiZCCHC6
DMDMi67462100

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001509571 – 1495Terminal uridylyltransferase 7Add BLAST1495

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57PhosphothreonineCombined sources1
Modified residuei64PhosphothreonineCombined sources1
Modified residuei132PhosphoserineBy similarity1
Modified residuei172PhosphoserineCombined sources1
Modified residuei600PhosphoserineCombined sources1
Modified residuei844PhosphoserineCombined sources1
Modified residuei893PhosphoserineBy similarity1
Modified residuei939PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5VYS8
MaxQBiQ5VYS8
PaxDbiQ5VYS8
PeptideAtlasiQ5VYS8
PRIDEiQ5VYS8
ProteomicsDBi65642
65643 [Q5VYS8-2]
65644 [Q5VYS8-3]
65645 [Q5VYS8-4]
65646 [Q5VYS8-5]
65647 [Q5VYS8-6]

PTM databases

iPTMnetiQ5VYS8
PhosphoSitePlusiQ5VYS8

Expressioni

Gene expression databases

BgeeiENSG00000083223 Expressed in 215 organ(s), highest expression level in tendon
CleanExiHS_ZCCHC6
ExpressionAtlasiQ5VYS8 baseline and differential
GenevisibleiQ5VYS8 HS

Organism-specific databases

HPAiHPA020615
HPA020620

Interactioni

Protein-protein interaction databases

BioGridi122795, 43 interactors
IntActiQ5VYS8, 18 interactors
STRINGi9606.ENSP00000365130

Structurei

Secondary structure

11495
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5VYS8
SMRiQ5VYS8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini551 – 600PAP-associated 1Add BLAST50
Domaini1233 – 1286PAP-associated 2Add BLAST54

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni951 – 1495Sufficient for monouridylation activity1 PublicationAdd BLAST545
Regioni1047 – 1050UTP bindingCombined sources4
Regioni1057 – 1060UTP bindingCombined sources4
Regioni1170 – 1174UTP bindingCombined sources5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 23Poly-Asp4
Compositional biasi815 – 936Glu-richAdd BLAST122
Compositional biasi1367 – 1370Poly-Arg4

Domaini

Utilizes two multidomain functional modules during the switch from monouridylation to oligouridylation. The catalytic module (containing the 3 CCHC-type Zinc finger domains) is essential for both activites while the Lin28-interacting module (LIM) at the N-termail part is indispensable for oligouridylation.1 Publication

Sequence similaritiesi

Belongs to the DNA polymerase type-B-like family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri244 – 274Matrin-typePROSITE-ProRule annotationAdd BLAST31
Zinc fingeri963 – 980CCHC-type 1PROSITE-ProRule annotationAdd BLAST18
Zinc fingeri1345 – 1362CCHC-type 2PROSITE-ProRule annotationCombined sources1 PublicationAdd BLAST18
Zinc fingeri1451 – 1468CCHC-type 3PROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2277 Eukaryota
COG5260 LUCA
GeneTreeiENSGT00550000074490
InParanoidiQ5VYS8
KOiK13291
OMAiKYFALPH
OrthoDBiEOG091G01G7
PhylomeDBiQ5VYS8
TreeFamiTF315661

Family and domain databases

InterProiView protein in InterPro
IPR003604 Matrin/U1-like-C_Znf_C2H2
IPR002058 PAP_assoc
IPR002934 Polymerase_NTP_transf_dom
IPR001878 Znf_CCHC
IPR036875 Znf_CCHC_sf
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF03828 PAP_assoc, 2 hits
PF00098 zf-CCHC, 3 hits
SMARTiView protein in SMART
SM00343 ZnF_C2HC, 3 hits
SM00451 ZnF_U1, 1 hit
SUPFAMiSSF57756 SSF57756, 3 hits
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 3 hits
PS00028 ZINC_FINGER_C2H2_1, 1 hit

Sequences (6+)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 6 described isoforms and 4 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: Q5VYS8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGDTAKPYFV KRTKDRGTMD DDDFRRGHPQ QDYLIIDDHA KGHGSKMEKG
60 70 80 90 100
LQKKKITPGN YGNTPRKGPC AVSSNPYAFK NPIYSQPAWM NDSHKDQSKR
110 120 130 140 150
WLSDEHTGNS DNWREFKPGP RIPVINRQRK DSFQENEDGY RWQDTRGCRT
160 170 180 190 200
VRRLFHKDLT SLETTSEMEA GSPENKKQRS RPRKPRKTRN EENEQDGDLE
210 220 230 240 250
GPVIDESVLS TKELLGLQQA EERLKRDCID RLKRRPRNYP TAKYTCRLCD
260 270 280 290 300
VLIESIAFAH KHIKEKRHKK NIKEKQEEEL LTTLPPPTPS QINAVGIAID
310 320 330 340 350
KVVQEFGLHN ENLEQRLEIK RIMENVFQHK LPDCSLRLYG SSCSRLGFKN
360 370 380 390 400
SDVNIDIQFP AIMSQPDVLL LVQECLKNSD SFIDVDADFH ARVPVVVCRE
410 420 430 440 450
KQSGLLCKVS AGNENACLTT KHLTALGKLE PKLVPLVIAF RYWAKLCSID
460 470 480 490 500
RPEEGGLPPY VFALMAIFFL QQRKEPLLPV YLGSWIEGFS LSKLGNFNLQ
510 520 530 540 550
DIEKDVVIWE HTDSAAGDTG ITKEEAPRET PIKRGQVSLI LDVKHQPSVP
560 570 580 590 600
VGQLWVELLR FYALEFNLAD LVISIRVKEL VSRELKDWPK KRIAIEDPYS
610 620 630 640 650
VKRNVARTLN SQPVFEYILH CLRTTYKYFA LPHKITKSSL LKPLNAITCI
660 670 680 690 700
SEHSKEVINH HPDVQTKDDK LKNSVLAQGP GATSSAANTC KVQPLTLKET
710 720 730 740 750
AESFGSPPKE EMGNEHISVH PENSDCIQAD VNSDDYKGDK VYHPETGRKN
760 770 780 790 800
EKEKVGRKGK HLLTVDQKRG EHVVCGSTRN NESESTLDLE GFQNPTAKEC
810 820 830 840 850
EGLATLDNKA DLDGESTEGT EELEDSLNHF THSVQGQTSE MIPSDEEEED
860 870 880 890 900
DEEEEEEEEP RLTINQREDE DGMANEDELD NTYTGSGDED ALSEEDDELG
910 920 930 940 950
EAAKYEDVKE CGKHVERALL VELNKISLKE ENVCEEKNSP VDQSDFFYEF
960 970 980 990 1000
SKLIFTKGKS PTVVCSLCKR EGHLKKDCPE DFKRIQLEPL PPLTPKFLNI
1010 1020 1030 1040 1050
LDQVCIQCYK DFSPTIIEDQ AREHIRQNLE SFIRQDFPGT KLSLFGSSKN
1060 1070 1080 1090 1100
GFGFKQSDLD VCMTINGLET AEGLDCVRTI EELARVLRKH SGLRNILPIT
1110 1120 1130 1140 1150
TAKVPIVKFF HLRSGLEVDI SLYNTLALHN TRLLSAYSAI DPRVKYLCYT
1160 1170 1180 1190 1200
MKVFTKMCDI GDASRGSLSS YAYTLMVLYF LQQRNPPVIP VLQEIYKGEK
1210 1220 1230 1240 1250
KPEIFVDGWN IYFFDQIDEL PTYWSECGKN TESVGQLWLG LLRFYTEEFD
1260 1270 1280 1290 1300
FKEHVISIRR KSLLTTFKKQ WTSKYIVIED PFDLNHNLGA GLSRKMTNFI
1310 1320 1330 1340 1350
MKAFINGRRV FGIPVKGFPK DYPSKMEYFF DPDVLTEGEL APNDRCCRIC
1360 1370 1380 1390 1400
GKIGHFMKDC PMRRKVRRRR DQEDALNQRY PENKEKRSKE DKEIHNKYTE
1410 1420 1430 1440 1450
REVSTKEDKP IQCTPQKAKP MRAAADLGRE KILRPPVEKW KRQDDKDLRE
1460 1470 1480 1490
KRCFICGREG HIKKECPQFK GSSGSLSSKY MTQGKASAKR TQQES
Length:1,495
Mass (Da):171,229
Last modified:December 7, 2004 - v1
Checksum:i1AAB0F5B37266FF9
GO
Isoform 2 (identifier: Q5VYS8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     597-615: DPYSVKRNVARTLNSQPVF → GISKCLSYSPPLFFLKVPV
     616-1495: Missing.

Note: No experimental confirmation available.
Show »
Length:615
Mass (Da):70,475
Checksum:iE3B7FEE22E438BBD
GO
Isoform 3 (identifier: Q5VYS8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     538-544: SLILDVK → VSSLLCR
     545-1495: Missing.

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):62,261
Checksum:iDE285E0532C43340
GO
Isoform 4 (identifier: Q5VYS8-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     363-486: MSQPDVLLLV...LLPVYLGSWI → I
     960-1072: Missing.

Note: No experimental confirmation available.
Show »
Length:1,259
Mass (Da):144,656
Checksum:i2DFDA89240222042
GO
Isoform 5 (identifier: Q5VYS8-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     403-412: SGLLCKVSAG → RSHFFKLFIY
     413-1495: Missing.

Note: No experimental confirmation available.
Show »
Length:412
Mass (Da):47,903
Checksum:i73F48669DDBD99CB
GO
Isoform 6 (identifier: Q5VYS8-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1157-1194: Missing.

Note: No experimental confirmation available.
Show »
Length:1,457
Mass (Da):166,970
Checksum:iF5F376C95EF2CEE5
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
X6R3Q3X6R3Q3_HUMAN
Terminal uridylyltransferase 7
TUT7
784Annotation score:
Q5VYS9Q5VYS9_HUMAN
Terminal uridylyltransferase 7
TUT7
168Annotation score:
A0A0C4DFW3A0A0C4DFW3_HUMAN
Terminal uridylyltransferase 7
TUT7
395Annotation score:
Q5VYT0Q5VYT0_HUMAN
Terminal uridylyltransferase 7
TUT7
124Annotation score:

Sequence cautioni

The sequence BAB14584 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB71052 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti157K → R in BAB70951 (PubMed:14702039).Curated1
Sequence conflicti316R → T in CAE46038 (PubMed:17974005).Curated1
Sequence conflicti514S → N in CAE46038 (PubMed:17974005).Curated1
Sequence conflicti900G → V in CAI45944 (PubMed:17974005).Curated1
Sequence conflicti937Missing in CAI45944 (PubMed:17974005).Curated1
Sequence conflicti937Missing in CAH56219 (PubMed:17974005).Curated1
Sequence conflicti1104V → M in CAH56219 (PubMed:17974005).Curated1
Sequence conflicti1319P → S in CAH56219 (PubMed:17974005).Curated1
Sequence conflicti1474G → D in CAI45944 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05375340A → V. Corresponds to variant dbSNP:rs2378695Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013832363 – 486MSQPD…LGSWI → I in isoform 4. 1 PublicationAdd BLAST124
Alternative sequenceiVSP_013833403 – 412SGLLCKVSAG → RSHFFKLFIY in isoform 5. 1 Publication10
Alternative sequenceiVSP_013834413 – 1495Missing in isoform 5. 1 PublicationAdd BLAST1083
Alternative sequenceiVSP_013835538 – 544SLILDVK → VSSLLCR in isoform 3. 1 Publication7
Alternative sequenceiVSP_013836545 – 1495Missing in isoform 3. 1 PublicationAdd BLAST951
Alternative sequenceiVSP_013837597 – 615DPYSV…SQPVF → GISKCLSYSPPLFFLKVPV in isoform 2. 1 PublicationAdd BLAST19
Alternative sequenceiVSP_013838616 – 1495Missing in isoform 2. 1 PublicationAdd BLAST880
Alternative sequenceiVSP_013839960 – 1072Missing in isoform 4. 1 PublicationAdd BLAST113
Alternative sequenceiVSP_0138401157 – 1194Missing in isoform 6. 1 PublicationAdd BLAST38

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL832026 mRNA Translation: CAH56219.1
AL832193 mRNA Translation: CAH56214.1
BX641077 mRNA Translation: CAE46038.1
CR933643 mRNA Translation: CAI45944.1
CR933644 mRNA Translation: CAI45945.1
CR936608 mRNA Translation: CAI56753.1
AL137849 Genomic DNA No translation available.
AL353678 Genomic DNA No translation available.
BC032456 mRNA Translation: AAH32456.1
AK023471 mRNA Translation: BAB14584.1 Different initiation.
AK055546 mRNA Translation: BAB70951.1
AK055948 mRNA Translation: BAB71052.1 Different initiation.
AB051498 mRNA Translation: BAB21802.1
CCDSiCCDS35057.1 [Q5VYS8-1]
CCDS55323.1 [Q5VYS8-4]
RefSeqiNP_001171988.1, NM_001185059.1 [Q5VYS8-1]
NP_001172003.1, NM_001185074.1 [Q5VYS8-4]
NP_001317647.1, NM_001330718.1
NP_078893.2, NM_024617.3 [Q5VYS8-1]
XP_016870619.1, XM_017015130.1 [Q5VYS8-1]
XP_016870620.1, XM_017015131.1 [Q5VYS8-1]
UniGeneiHs.597057

Genome annotation databases

EnsembliENST00000375960; ENSP00000365127; ENSG00000083223 [Q5VYS8-4]
ENST00000375963; ENSP00000365130; ENSG00000083223 [Q5VYS8-1]
GeneIDi79670
KEGGihsa:79670
UCSCiuc004aoq.4 human [Q5VYS8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTUT7_HUMAN
AccessioniPrimary (citable) accession number: Q5VYS8
Secondary accession number(s): Q5H9T0
, Q5VYS5, Q5VYS7, Q658Z9, Q659A2, Q6MZJ3, Q8N5F0, Q96N57, Q96NE8, Q9C0F2, Q9H8M6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: December 7, 2004
Last modified: September 12, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health

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