UniProtKB - Q5VWP2 (TET5C_HUMAN)
Terminal nucleotidyltransferase 5C
TENT5C
Functioni
Catalyzes the transfer of one adenosine molecule from an ATP to an mRNA poly(A) tail bearing a 3'-OH terminal group and enhances mRNA stability and gene expression (PubMed:32009146, PubMed:28931820, PubMed:34048638).
Can also elongate RNA oligos ending with uridine molecule, provided that the sequence is adenosine-rich (PubMed:34048638).
Mainly targets mRNAs encoding endoplasmic reticulum-targeted protein (PubMed:28931820).
3 Publications(Microbial infection) Seems to enhance replication of some viruses, including yellow fever virus, in response to type I interferon.
1 PublicationCatalytic activityi
GO - Molecular functioni
- polynucleotide adenylyltransferase activity Source: UniProtKB
- RNA adenylyltransferase activity Source: UniProtKB
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- in utero embryonic development Source: Ensembl
- mRNA stabilization Source: UniProtKB
- negative regulation of cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Nucleotidyltransferase, RNA-binding, Transferase |
Biological process | Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | Q5VWP2 |
SignaLinki | Q5VWP2 |
Names & Taxonomyi
Protein namesi | Recommended name: Terminal nucleotidyltransferase 5CCurated (EC:2.7.7.192 Publications)Alternative name(s): Non-canonical poly(A) polymerase FAM46C1 Publication |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:24712, TENT5C |
MIMi | 613952, gene |
neXtProti | NX_Q5VWP2 |
VEuPathDBi | HostDB:ENSG00000183508 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- centrosome 1 Publication
Note: Recruited into the centrosome through its interaction with PLK4.1 Publication
Cytoskeleton
- centrosome Source: UniProtKB
Nucleus
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 90 – 92 | DLD → ALA: Loss of poly(a) polymerase activity. 1 Publication | 3 | |
Mutagenesisi | 144 | K → P: Decreases substantially the interaction with PLK4. Weakens binding to PLK4; when associated with E-230 and E-321. Abolishes the inhibitory effect of TENT5C on the cell viability; when associated with E-320 and E-321. 1 Publication | 1 | |
Mutagenesisi | 146 | C → P: Decreases substantially the interaction with PLK4. Weakens binding to PLK4; when associated with E-230 and E-321. 1 Publication | 1 | |
Mutagenesisi | 166 | E → Q: Does not affect colocalization with PLK4 in centrosome. Increases cell viability. 1 Publication | 1 | |
Mutagenesisi | 320 | C → E: Slightly decreases the binding to PLK4; when associated with E-321. Abolishes the inhibitory effect of TENT5C on the cell viability; when associated with P-144 and E-321. 1 Publication | 1 | |
Mutagenesisi | 321 | L → E: Slightly decreases the binding to PLK4; when associated with E-320. Abolishes the inhibitory effect of TENT5C on the cell viability; when associated with P-144 and E-320. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 54855 |
OpenTargetsi | ENSG00000183508 |
PharmGKBi | PA134955264 |
Miscellaneous databases
Pharosi | Q5VWP2, Tbio |
Genetic variation databases
BioMutai | FAM46C |
DMDMi | 117940143 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000259933 | 1 – 391 | Terminal nucleotidyltransferase 5CAdd BLAST | 391 |
Proteomic databases
jPOSTi | Q5VWP2 |
MassIVEi | Q5VWP2 |
PaxDbi | Q5VWP2 |
PeptideAtlasi | Q5VWP2 |
PRIDEi | Q5VWP2 |
ProteomicsDBi | 65547 |
PTM databases
iPTMneti | Q5VWP2 |
PhosphoSitePlusi | Q5VWP2 |
Expressioni
Inductioni
Gene expression databases
Bgeei | ENSG00000183508, Expressed in secondary oocyte and 208 other tissues |
Genevisiblei | Q5VWP2, HS |
Organism-specific databases
HPAi | ENSG00000183508, Tissue enhanced (bone) |
Interactioni
Subunit structurei
Binary interactionsi
Q5VWP2
With | #Exp. | IntAct |
---|---|---|
RHOXF2 [Q9BQY4] | 3 | EBI-741787,EBI-372094 |
Protein-protein interaction databases
BioGRIDi | 120205, 11 interactors |
IntActi | Q5VWP2, 9 interactors |
STRINGi | 9606.ENSP00000358458 |
Miscellaneous databases
RNActi | Q5VWP2, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q5VWP2 |
SMRi | Q5VWP2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3852, Eukaryota |
GeneTreei | ENSGT00940000158856 |
HOGENOMi | CLU_008115_2_0_1 |
InParanoidi | Q5VWP2 |
OMAi | NTRDCMS |
OrthoDBi | 612201at2759 |
PhylomeDBi | Q5VWP2 |
TreeFami | TF315239 |
Family and domain databases
InterProi | View protein in InterPro IPR012937, TET5 |
PANTHERi | PTHR12974, PTHR12974, 1 hit |
Pfami | View protein in Pfam PF07984, NTP_transf_7, 1 hit |
SMARTi | View protein in SMART SM01153, DUF1693, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAEESSCTRD CMSFSVLNWD QVSRLHEVLT EVVPIHGRGN FPTLEITLKD
60 70 80 90 100
IVQTVRSRLE EAGIKVHDVR LNGSAAGHVL VKDNGLGCKD LDLIFHVALP
110 120 130 140 150
TEAEFQLVRD VVLCSLLNFL PEGVNKLKIS PVTLKEAYVQ KLVKVCTDTD
160 170 180 190 200
RWSLISLSNK NGKNVELKFV DSIRRQFEFS VDSFQIILDS LLFFYDCSNN
210 220 230 240 250
PISEHFHPTV IGESMYGDFE EAFDHLQNRL IATKNPEEIR GGGLLKYSNL
260 270 280 290 300
LVRDFRPTDQ EEIKTLERYM CSRFFIDFPD ILEQQRKLET YLQNHFAEEE
310 320 330 340 350
RSKYDYLMIL RRVVNESTVC LMGHERRQTL NLISLLALRV LAEQNIIPSA
360 370 380 390
TNVTCYYQPA PYVSDGNFSN YYVAHPPVTY SQPYPTWLPC N
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 150 | D → V in BAA91010 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_060132 | 67 | H → Q. Corresponds to variant dbSNP:rs1630312EnsemblClinVar. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK000209 mRNA Translation: BAA91010.1 AL365331 Genomic DNA No translation available. BC036516 mRNA Translation: AAH36516.3 BC131726 mRNA Translation: AAI31727.1 |
CCDSi | CCDS896.1 |
RefSeqi | NP_060179.2, NM_017709.3 |
Genome annotation databases
Ensembli | ENST00000369448.4; ENSP00000358458.3; ENSG00000183508.5 |
GeneIDi | 54855 |
KEGGi | hsa:54855 |
MANE-Selecti | ENST00000369448.4; ENSP00000358458.3; NM_017709.4; NP_060179.2 |
UCSCi | uc001ehe.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK000209 mRNA Translation: BAA91010.1 AL365331 Genomic DNA No translation available. BC036516 mRNA Translation: AAH36516.3 BC131726 mRNA Translation: AAI31727.1 |
CCDSi | CCDS896.1 |
RefSeqi | NP_060179.2, NM_017709.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
6W36 | X-ray | 2.85 | A | 14-358 | [»] | |
6W38 | X-ray | 4.48 | A | 14-358 | [»] | |
6W3I | X-ray | 3.80 | A | 14-358 | [»] | |
6W3J | X-ray | 4.38 | A | 14-358 | [»] | |
AlphaFoldDBi | Q5VWP2 | |||||
SMRi | Q5VWP2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 120205, 11 interactors |
IntActi | Q5VWP2, 9 interactors |
STRINGi | 9606.ENSP00000358458 |
PTM databases
iPTMneti | Q5VWP2 |
PhosphoSitePlusi | Q5VWP2 |
Genetic variation databases
BioMutai | FAM46C |
DMDMi | 117940143 |
Proteomic databases
jPOSTi | Q5VWP2 |
MassIVEi | Q5VWP2 |
PaxDbi | Q5VWP2 |
PeptideAtlasi | Q5VWP2 |
PRIDEi | Q5VWP2 |
ProteomicsDBi | 65547 |
Protocols and materials databases
Antibodypediai | 33893, 108 antibodies from 20 providers |
DNASUi | 54855 |
Genome annotation databases
Ensembli | ENST00000369448.4; ENSP00000358458.3; ENSG00000183508.5 |
GeneIDi | 54855 |
KEGGi | hsa:54855 |
MANE-Selecti | ENST00000369448.4; ENSP00000358458.3; NM_017709.4; NP_060179.2 |
UCSCi | uc001ehe.4, human |
Organism-specific databases
CTDi | 54855 |
DisGeNETi | 54855 |
GeneCardsi | TENT5C |
HGNCi | HGNC:24712, TENT5C |
HPAi | ENSG00000183508, Tissue enhanced (bone) |
MIMi | 613952, gene |
neXtProti | NX_Q5VWP2 |
OpenTargetsi | ENSG00000183508 |
PharmGKBi | PA134955264 |
VEuPathDBi | HostDB:ENSG00000183508 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3852, Eukaryota |
GeneTreei | ENSGT00940000158856 |
HOGENOMi | CLU_008115_2_0_1 |
InParanoidi | Q5VWP2 |
OMAi | NTRDCMS |
OrthoDBi | 612201at2759 |
PhylomeDBi | Q5VWP2 |
TreeFami | TF315239 |
Enzyme and pathway databases
PathwayCommonsi | Q5VWP2 |
SignaLinki | Q5VWP2 |
Miscellaneous databases
BioGRID-ORCSi | 54855, 10 hits in 1078 CRISPR screens |
GenomeRNAii | 54855 |
Pharosi | Q5VWP2, Tbio |
PROi | PR:Q5VWP2 |
RNActi | Q5VWP2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000183508, Expressed in secondary oocyte and 208 other tissues |
Genevisiblei | Q5VWP2, HS |
Family and domain databases
InterProi | View protein in InterPro IPR012937, TET5 |
PANTHERi | PTHR12974, PTHR12974, 1 hit |
Pfami | View protein in Pfam PF07984, NTP_transf_7, 1 hit |
SMARTi | View protein in SMART SM01153, DUF1693, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TET5C_HUMAN | |
Accessioni | Q5VWP2Primary (citable) accession number: Q5VWP2 Secondary accession number(s): A3KMG2, Q8NE25, Q9NXK0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 14, 2006 |
Last sequence update: | August 30, 2005 | |
Last modified: | May 25, 2022 | |
This is version 119 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families