TAF3

Functionⁱ

Transcription factor TFIID is one of the general factors required for accurate and regulated initiation by RNA polymerase II. TFIID is a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Required in complex with TBPL2 for the differentiation of myoblasts into myocytes. The complex replaces TFIID at specific promoters at an early stage in the differentiation process.

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	865 – 915	PHD-typePROSITE-ProRule annotationAdd BLAST		51

GO - Molecular functionⁱ

metal ion binding Source: UniProtKB-KW
p53 binding Source: Ensembl
protein heterodimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

maintenance of protein location in nucleus
Source: HGNC
Inferred from direct assayⁱ
- 15870280
negative regulation of DNA-binding transcription factor activity
Source: BHF-UCL
Inferred from direct assayⁱ
- 18549481
negative regulation of transcription by RNA polymerase II
Source: BHF-UCL
Inferred from direct assayⁱ
- 18549481
regulation of signal transduction by p53 class mediator Source: Reactome
transcription by RNA polymerase II Source: Reactome
transcription initiation from RNA polymerase II promoter Source: Reactome

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Transcription, Transcription regulation
Ligand	Metal-binding, Zinc

Enzyme and pathway databases

Reactomeⁱ	R-HSA-167161 HIV Transcription Initiation R-HSA-167162 RNA Polymerase II HIV Promoter Escape R-HSA-167172 Transcription of the HIV genome R-HSA-674695 RNA Polymerase II Pre-transcription Events R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation R-HSA-73776 RNA Polymerase II Promoter Escape R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening R-HSA-75953 RNA Polymerase II Transcription Initiation R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
SIGNORⁱ	Q5VWG9

Reactomeⁱ

R-HSA-167161 HIV Transcription Initiation
R-HSA-167162 RNA Polymerase II HIV Promoter Escape
R-HSA-167172 Transcription of the HIV genome
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-73776 RNA Polymerase II Promoter Escape
R-HSA-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-HSA-75953 RNA Polymerase II Transcription Initiation
R-HSA-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance

SIGNORⁱ

Q5VWG9

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Transcription initiation factor TFIID subunit 3 Alternative name(s): 140 kDa TATA box-binding protein-associated factor TBP-associated factor 3 Transcription initiation factor TFIID 140 kDa subunit Short name: TAF(II)140 Short name: TAF140 Short name: TAFII-140 Short name: TAFII140
Gene namesⁱ	Name:TAF3
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 10

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000165632.7
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:17303 TAF3
MIMⁱ	606576 gene
neXtProtⁱ	NX_Q5VWG9

Keywords - Cellular componentⁱ

Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	23	W → R: Loss of interaction with TAF10. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	83860
Open Targets More...OpenTargetsⁱ	ENSG00000165632
PharmGKBⁱ	PA38222

Polymorphism and mutation databases

DMDMⁱ	74747393

DMDMⁱ

74747393

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000245528	1 – 929	Transcription initiation factor TFIID subunit 3Add BLAST		929

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	183	PhosphoserineCombined sources	1
Modified residueⁱ	199	PhosphoserineCombined sources	1
Modified residueⁱ	229	PhosphoserineCombined sources	1
Modified residueⁱ	243	PhosphoserineCombined sources	1
Modified residueⁱ	266	N6-acetyllysineCombined sources	1
Modified residueⁱ	291	PhosphoserineCombined sources	1
Modified residueⁱ	297	PhosphoserineCombined sources	1
Modified residueⁱ	301	PhosphoserineCombined sources	1
Modified residueⁱ	501	PhosphothreonineCombined sources	1
Cross-linkⁱ	581	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residueⁱ	667	PhosphoserineCombined sources	1
Cross-linkⁱ	746	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residueⁱ	755	PhosphoserineCombined sources	1
Modified residueⁱ	776	N6-acetyllysineCombined sources	1

Keywords - PTMⁱ

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q5VWG9
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q5VWG9
PaxDbⁱ	Q5VWG9
PeptideAtlas More...PeptideAtlasⁱ	Q5VWG9
PRIDEⁱ	Q5VWG9
ProteomicsDBⁱ	65526

PTM databases

iPTMnetⁱ	Q5VWG9
PhosphoSitePlusⁱ	Q5VWG9

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000165632 Expressed in 210 organ(s), highest expression level in tendon of biceps brachii
CleanExⁱ	HS_TAF3
Genevisibleⁱ	Q5VWG9 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA066184

HPAⁱ

HPA066184

Interactionⁱ

Subunit structureⁱ

Belongs to the TFIID complex which is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). Interacts with TAF10 via the histone fold. Interacts with TAF13, TBP, SAP130 and GCN5L2. Interacts with TBPL2.1 Publication

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
H3F3B	P84243	3	EBI-1560087,EBI-120658

With

Entry

#Exp.

IntAct

Notes

H3F3B

P84243

3

EBI-1560087,EBI-120658

GO - Molecular functionⁱ

p53 binding Source: Ensembl
protein heterodimerization activity Source: InterPro

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	123775, 29 interactors
ComplexPortalⁱ	CPX-915 General transcription factor complex TFIID CPX-930 General transcription factor complex TFIID, TAF4B variant
CORUMⁱ	Q5VWG9
Database of interacting proteins More...DIPⁱ	DIP-38920N
IntActⁱ	Q5VWG9, 6 interactors
Molecular INTeraction database More...MINTⁱ	Q5VWG9
STRINGⁱ	9606.ENSP00000340271

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	856 – 858	Combined sources	3
Beta strandⁱ	864 – 867	Combined sources	4
Turnⁱ	869 – 871	Combined sources	3
Beta strandⁱ	880 – 882	Combined sources	3
Turnⁱ	884 – 886	Combined sources	3
Beta strandⁱ	889 – 891	Combined sources	3
Helixⁱ	892 – 895	Combined sources	4
Helixⁱ	910 – 913	Combined sources	4

Show more details

3D structure databases

ProteinModelPortalⁱ	Q5VWG9
SMRⁱ	Q5VWG9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Compositional bias

Feature key	Position(s)	DescriptionActions	Length
Compositional biasⁱ	136 – 141	Poly-Glu	6
Compositional biasⁱ	163 – 168	Poly-Glu	6
Compositional biasⁱ	507 – 746	Lys-richAdd BLAST	240
Compositional biasⁱ	779 – 833	Pro-richAdd BLAST	55

Domainⁱ

The PHD-type zinc finger mediates binding to histone H3 methyllysine at position 4 (H3K4me3).By similarity

Sequence similaritiesⁱ

Belongs to the TAF3 family.Curated

Zinc finger

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Zinc fingerⁱ	865 – 915	PHD-typePROSITE-ProRule annotationAdd BLAST		51

Keywords - Domainⁱ

Zinc-finger

Phylogenomic databases

eggNOGⁱ	KOG1973 Eukaryota KOG2389 Eukaryota ENOG410ZS4D LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00710000106806
HOGENOMⁱ	HOG000231905
HOVERGENⁱ	HBG083188
InParanoidⁱ	Q5VWG9
KEGG Orthology (KO) More...KOⁱ	K14650
OMAⁱ	MPPNFPY
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0XX7
PhylomeDBⁱ	Q5VWG9
TreeFamⁱ	TF316513

Family and domain databases

Gene3Dⁱ	1.10.20.10, 1 hit 3.30.40.10, 1 hit
InterProⁱ	View protein in InterPro IPR006565 BTP IPR009072 Histone-fold IPR037815 TAF3 IPR019786 Zinc_finger_PHD-type_CS IPR011011 Znf_FYVE_PHD IPR001965 Znf_PHD IPR019787 Znf_PHD-finger IPR013083 Znf_RING/FYVE/PHD
PANTHERⁱ	PTHR12321:SF34 PTHR12321:SF34, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07524 Bromo_TP, 1 hit PF00628 PHD, 1 hit
SMARTⁱ	View protein in SMART SM00576 BTP, 1 hit SM00249 PHD, 1 hit
SUPFAMⁱ	SSF57903 SSF57903, 1 hit
PROSITEⁱ	View protein in PROSITE PS01359 ZF_PHD_1, 1 hit PS50016 ZF_PHD_2, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q5VWG9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MCESYSRSLL RVSVAQICQA LGWDSVQLSA CHLLTDVLQR YLQQLGRGCH 
        60         70         80         90        100
RYSELYGRTD PILDDVGEAF QLMGVSLHEL EDYIHNIEPV TFPHQIPSFP 
       110        120        130        140        150
VSKNNVLQFP QPGSKDAEER KEYIPDYLPP IVSSQEEEEE EQVPTDGGTS 
       160        170        180        190        200
AEAMQVPLEE DDELEEEEII NDENFLGKRP LDSPEAEELP AMKRPRLLST 
       210        220        230        240        250
KGDTLDVVLL EAREPLSSIN TQKIPPMLSP VHVQDSTDLA PPSPEPPMLA 
       260        270        280        290        300
PVAKSQMPTA KPLETKSFTP KTKTKTSSPG QKTKSPKTAQ SPAMVGSPIR 
       310        320        330        340        350
SPKTVSKEKK SPGRSKSPKS PKSPKVTTHI PQTPVRPETP NRTPSATLSE 
       360        370        380        390        400
KISKETIQVK QIQTPPDAGK LNSENQPKKA VVADKTIEAS IDAVIARACA 
       410        420        430        440        450
EREPDPFEFS SGSESEGDIF TSPKRISGPE CTTPKASTSA NNFTKSGSTP 
       460        470        480        490        500
LPLSGGTSSS DNSWTMDASI DEVVRKAKLG TPSNMPPNFP YISSPSVSPP 
       510        520        530        540        550
TPEPLHKVYE EKTKLPSSVE VKKKLKKELK TKMKKKEKQR DREREKDKNK 
       560        570        580        590        600
DKSKEKDKVK EKEKDKETGR ETKYPWKEFL KEEEADPYKF KIKEFEDVDP 
       610        620        630        640        650
KVKLKDGLVR KEKEKHKDKK KDREKGKKDK DKREKEKVKD KGREDKMKAP 
       660        670        680        690        700
APPLVLPPKE LALPLFSPAT ASRVPAMLPS LLPVLPEKLF EEKEKVKEKE 
       710        720        730        740        750
KKKDKKEKKK KKEKEKEKKE KEREKEKRER EKREKEKEKH KHEKIKVEPV 
       760        770        780        790        800
ALAPSPVIPR LTLRVGAGQD KIVISKVVPA PEAKPAPSQN RPKTPPPAPA 
       810        820        830        840        850
PAPGPMLVSP APVPLPLLAQ AAAGPALLPS PGPAASGASA KAPVRSVVTE 
       860        870        880        890        900
TVSTYVIRDE WGNQIWICPG CNKPDDGSPM IGCDDCDDWY HWPCVGIMTA 
       910        920 
PPEEMQWFCP KCANKKKDKK HKKRKHRAH

Length:929

Mass (Da):103,582

Last modified:December 7, 2004 - v1

Checksum:ⁱC951754B5B532E72

GO

Sequence cautionⁱ

The sequence AAH45106 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

The sequence AAH62352 differs from that shown. Reason: Erroneous initiation.Curated

The sequence AAH73884 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	23 – 25	WDS → TRP in CAB56032 (PubMed:17974005).Curated	3
Sequence conflictⁱ	533	M → K in AAH73884 (PubMed:15489334).Curated	1
Sequence conflictⁱ	537 – 539	EKQ → KKK in AAH17320 (PubMed:15489334).Curated	3
Sequence conflictⁱ	696	V → P in AAH62352 (PubMed:15489334).Curated	1
Sequence conflictⁱ	700	E → K in CAB56032 (PubMed:17974005).Curated	1
Sequence conflictⁱ	707	E → K in AAH62352 (PubMed:15489334).Curated	1
Sequence conflictⁱ	713	E → K in AAH62352 (PubMed:15489334).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_052254	349	S → T. Corresponds to variant dbSNP:rs17366712Ensembl.	1
Natural variantⁱVAR_052255	442	N → S1 PublicationCorresponds to variant dbSNP:rs4747647Ensembl.	1
Natural variantⁱVAR_052256	696	V → A1 PublicationCorresponds to variant dbSNP:rs1244229Ensembl.	1
Natural variantⁱVAR_052257	696	V → L. Corresponds to variant dbSNP:rs10795583Ensembl.	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ292190 mRNA Translation: CAC34475.1 AL390294 Genomic DNA No translation available. AL353754 Genomic DNA No translation available. AL159172 Genomic DNA No translation available. BC017320 mRNA Translation: AAH17320.1 BC045106 mRNA Translation: AAH45106.1 Sequence problems. BC073884 mRNA Translation: AAH73884.1 Different initiation. BC062352 mRNA Translation: AAH62352.1 Different initiation. AL117661 mRNA Translation: CAB56032.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS41487.1
PIRⁱ	T17342
NCBI Reference Sequences More...RefSeqⁱ	NP_114129.1, NM_031923.3
UniGeneⁱ	Hs.527688 Hs.709995

Genome annotation databases

Ensemblⁱ	ENST00000344293; ENSP00000340271; ENSG00000165632
GeneIDⁱ	83860
KEGGⁱ	hsa:83860
UCSC genome browser More...UCSCⁱ	uc010qbd.4 human

Keywords - Coding sequence diversityⁱ

Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q5VWG9	TATA-box binding protein associated factor 3		CALJA		1099	UniRef90_Q5VWG9
TATA-box binding protein associated factor 3		MACFA		1008
Transcription initiation factor TFIID subunit 3 (Fragment)		MACMU		73
TATA-box binding protein associated factor 3		PONAB		933
TATA-box binding protein associated factor 3		CERAT		931
+38

Protein	Similar proteins		Species	Score	Length	Source
Q5VWG9	TATA-box binding protein associated factor 3		CALJA		1099	UniRef50_Q5VWG9
TATA-box binding protein associated factor 3		MACFA		1008
Transcription initiation factor TFIID subunit 3 (Fragment)		MACMU		73
TATA-box binding protein associated factor 3		PONAB		933
TATA-box binding protein associated factor 3		PAPAN		931
+39

Entry informationⁱ

Entry nameⁱ	TAF3_HUMAN
Accessionⁱ	Q5VWG9Primary (citable) accession number: Q5VWG9 Secondary accession number(s): Q05DA0 , Q6GMS5, Q6P6B5, Q86VY6, Q9BQS9, Q9UFI8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 11, 2006
	Last sequence update:	December 7, 2004
	Last modified:	September 12, 2018
	This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

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UniRef

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Annotation systems

Supporting data

UniProtKB - Q5VWG9 (TAF3_HUMAN)

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Transcription initiation factor TFIID subunit 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Zinc finger

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ