UniProtKB - Q5VWC8 (HACD4_HUMAN)
Protein
Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4
Gene
HACD4
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the third of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.1 Publication
Caution
Shares some similarity with tyrosine phosphatase proteins but it has probably no phosphatase activity.By similarity1 Publication
Catalytic activityi
- EC:4.2.1.1341 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Kineticsi
- KM=6.8 µM for 3-hydroxypalmitoyl-CoA (at 37 degrees Celsius)1 Publication
: fatty acid biosynthesis Pathwayi
This protein is involved in the pathway fatty acid biosynthesis, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway fatty acid biosynthesis and in Lipid metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 156 | By similarity | 1 | |
Active sitei | 163 | By similarity | 1 |
GO - Molecular functioni
- 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB
- 3-hydroxy-arachidoyl-CoA dehydratase activity Source: UniProtKB-EC
- 3-hydroxy-behenoyl-CoA dehydratase activity Source: UniProtKB-EC
- 3-hydroxy-lignoceroyl-CoA dehydratase activity Source: UniProtKB-EC
- enzyme binding Source: UniProtKB
- very-long-chain 3-hydroxyacyl-CoA dehydratase activity Source: UniProtKB-EC
GO - Biological processi
- fatty acid elongation Source: UniProtKB
- sphingolipid biosynthetic process Source: GO_Central
- very long-chain fatty acid biosynthetic process Source: UniProtKB
Keywordsi
Molecular function | Lyase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
PathwayCommonsi | Q5VWC8 |
Reactomei | R-HSA-75876, Synthesis of very long-chain fatty acyl-CoAs |
UniPathwayi | UPA00094 |
Chemistry databases
SwissLipidsi | SLP:000000440 |
Names & Taxonomyi
Protein namesi | Recommended name: Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4Curated (EC:4.2.1.1341 Publication)Alternative name(s): 3-hydroxyacyl-CoA dehydratase 41 Publication Short name: HACD41 Publication Protein-tyrosine phosphatase-like A domain-containing protein 2Imported |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000188921.13 |
HGNCi | HGNC:20920, HACD4 |
MIMi | 615941, gene |
neXtProti | NX_Q5VWC8 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB
- integral component of endoplasmic reticulum membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 19 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 20 – 40 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 41 – 56 | LumenalSequence analysisAdd BLAST | 16 | |
Transmembranei | 57 – 77 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 78 – 112 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 113 – 133 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 134 – 135 | LumenalSequence analysis | 2 | |
Transmembranei | 136 – 156 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 157 | CytoplasmicSequence analysis | 1 | |
Transmembranei | 158 – 178 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 179 – 189 | LumenalSequence analysisAdd BLAST | 11 | |
Transmembranei | 190 – 210 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 211 – 232 | CytoplasmicSequence analysisAdd BLAST | 22 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 401494 |
OpenTargetsi | ENSG00000188921 |
PharmGKBi | PA142671114 |
Miscellaneous databases
Pharosi | Q5VWC8, Tbio |
Polymorphism and mutation databases
BioMutai | HACD4 |
DMDMi | 74747375 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000313730 | 1 – 232 | Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 4Add BLAST | 232 |
Proteomic databases
jPOSTi | Q5VWC8 |
MassIVEi | Q5VWC8 |
PaxDbi | Q5VWC8 |
PeptideAtlasi | Q5VWC8 |
PRIDEi | Q5VWC8 |
ProteomicsDBi | 65525 |
TopDownProteomicsi | Q5VWC8 |
PTM databases
PhosphoSitePlusi | Q5VWC8 |
Expressioni
Tissue specificityi
Highly expressed in leukocytes, and low expression in heart, spleen, kidney, and placenta.1 Publication
Gene expression databases
Bgeei | ENSG00000188921, Expressed in bone marrow and 169 other tissues |
Genevisiblei | Q5VWC8, HS |
Organism-specific databases
HPAi | ENSG00000188921, Low tissue specificity |
Interactioni
Subunit structurei
May interact with enzymes of the ELO family (including ELOVL1); with those enzymes that mediate condensation, the first of the four steps of the reaction cycle responsible for fatty acids elongation, may be part of a larger fatty acids elongase complex.
1 PublicationBinary interactionsi
Q5VWC8
With | #Exp. | IntAct |
---|---|---|
CD79A [P11912] | 3 | EBI-18076069,EBI-7797864 |
GO - Molecular functioni
- enzyme binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 135110, 1 interactor |
IntActi | Q5VWC8, 1 interactor |
STRINGi | 9606.ENSP00000419503 |
Miscellaneous databases
RNActi | Q5VWC8, protein |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 228 – 231 | Poly-Lys | 4 |
Sequence similaritiesi
Belongs to the very long-chain fatty acids dehydratase HACD family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3187, Eukaryota |
GeneTreei | ENSGT00530000062962 |
HOGENOMi | CLU_034302_3_0_1 |
InParanoidi | Q5VWC8 |
OMAi | IGMYYTY |
OrthoDBi | 1458293at2759 |
PhylomeDBi | Q5VWC8 |
TreeFami | TF313326 |
Family and domain databases
InterProi | View protein in InterPro IPR007482, Tyr_Pase-like_PTPLA |
PANTHERi | PTHR11035, PTHR11035, 1 hit |
Pfami | View protein in Pfam PF04387, PTPLA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5VWC8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGPLALPAWL QPRYRKNAYL FIYYLIQFCG HSWIFTNMTV RFFSFGKDSM
60 70 80 90 100
VDTFYAIGLV MRLCQSVSLL ELLHIYVGIE SNHLLPRFLQ LTERIIILFV
110 120 130 140 150
VITSQEEVQE KYVVCVLFVF WNLLDMVRYT YSMLSVIGIS YAVLTWLSQT
160 170 180 190 200
LWMPIYPLCV LAEAFAIYQS LPYFESFGTY STKLPFDLSI YFPYVLKIYL
210 220 230
MMLFIGMYFT YSHLYSERRD ILGIFPIKKK KM
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 133 | M → V in CAD97990 (PubMed:17974005).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_037714 | 36 | T → A. Corresponds to variant dbSNP:rs2298260Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL390882 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58626.1 BC114215 mRNA Translation: AAI14216.1 BX538052 mRNA Translation: CAD97990.1 |
CCDSi | CCDS43791.1 |
RefSeqi | NP_001010915.2, NM_001010915.4 NP_001308812.1, NM_001321883.1 |
Genome annotation databases
Ensembli | ENST00000495827; ENSP00000419503; ENSG00000188921 |
GeneIDi | 401494 |
KEGGi | hsa:401494 |
UCSCi | uc010miq.3, human |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL390882 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58626.1 BC114215 mRNA Translation: AAI14216.1 BX538052 mRNA Translation: CAD97990.1 |
CCDSi | CCDS43791.1 |
RefSeqi | NP_001010915.2, NM_001010915.4 NP_001308812.1, NM_001321883.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 135110, 1 interactor |
IntActi | Q5VWC8, 1 interactor |
STRINGi | 9606.ENSP00000419503 |
Chemistry databases
SwissLipidsi | SLP:000000440 |
PTM databases
PhosphoSitePlusi | Q5VWC8 |
Polymorphism and mutation databases
BioMutai | HACD4 |
DMDMi | 74747375 |
Proteomic databases
jPOSTi | Q5VWC8 |
MassIVEi | Q5VWC8 |
PaxDbi | Q5VWC8 |
PeptideAtlasi | Q5VWC8 |
PRIDEi | Q5VWC8 |
ProteomicsDBi | 65525 |
TopDownProteomicsi | Q5VWC8 |
Protocols and materials databases
Antibodypediai | 24843, 31 antibodies |
Genome annotation databases
Ensembli | ENST00000495827; ENSP00000419503; ENSG00000188921 |
GeneIDi | 401494 |
KEGGi | hsa:401494 |
UCSCi | uc010miq.3, human |
Organism-specific databases
CTDi | 401494 |
DisGeNETi | 401494 |
EuPathDBi | HostDB:ENSG00000188921.13 |
GeneCardsi | HACD4 |
HGNCi | HGNC:20920, HACD4 |
HPAi | ENSG00000188921, Low tissue specificity |
MIMi | 615941, gene |
neXtProti | NX_Q5VWC8 |
OpenTargetsi | ENSG00000188921 |
PharmGKBi | PA142671114 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3187, Eukaryota |
GeneTreei | ENSGT00530000062962 |
HOGENOMi | CLU_034302_3_0_1 |
InParanoidi | Q5VWC8 |
OMAi | IGMYYTY |
OrthoDBi | 1458293at2759 |
PhylomeDBi | Q5VWC8 |
TreeFami | TF313326 |
Enzyme and pathway databases
UniPathwayi | UPA00094 |
PathwayCommonsi | Q5VWC8 |
Reactomei | R-HSA-75876, Synthesis of very long-chain fatty acyl-CoAs |
Miscellaneous databases
BioGRID-ORCSi | 401494, 6 hits in 822 CRISPR screens |
ChiTaRSi | HACD4, human |
GenomeRNAii | 401494 |
Pharosi | Q5VWC8, Tbio |
PROi | PR:Q5VWC8 |
RNActi | Q5VWC8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000188921, Expressed in bone marrow and 169 other tissues |
Genevisiblei | Q5VWC8, HS |
Family and domain databases
InterProi | View protein in InterPro IPR007482, Tyr_Pase-like_PTPLA |
PANTHERi | PTHR11035, PTHR11035, 1 hit |
Pfami | View protein in Pfam PF04387, PTPLA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HACD4_HUMAN | |
Accessioni | Q5VWC8Primary (citable) accession number: Q5VWC8 Secondary accession number(s): Q7Z385 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | December 7, 2004 | |
Last modified: | December 2, 2020 | |
This is version 111 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations