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Protein

Ubiquitin-conjugating enzyme E2 U

Gene

UBE2U

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.1 Publication

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ5VVX9
UniPathwayi
UPA00143

Protein family/group databases

MoonDBiQ5VVX9 Predicted

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 U (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme U
Ubiquitin carrier protein U
Ubiquitin-protein ligase U
Gene namesi
Name:UBE2U
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000177414.13
HGNCiHGNC:28559 UBE2U
neXtProtiNX_Q5VVX9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000177414
PharmGKBiPA142670656

Polymorphism and mutation databases

BioMutaiUBE2U
DMDMi74747311

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825121 – 321Ubiquitin-conjugating enzyme E2 UAdd BLAST321

Post-translational modificationi

Autoubiquitinated in vitro in the presence of UBR5.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

PaxDbiQ5VVX9
PRIDEiQ5VVX9
ProteomicsDBi65500
65501 [Q5VVX9-2]

PTM databases

iPTMnetiQ5VVX9
PhosphoSitePlusiQ5VVX9

Expressioni

Gene expression databases

BgeeiENSG00000177414 Expressed in 49 organ(s), highest expression level in right testis
CleanExiHS_UBE2U
ExpressionAtlasiQ5VVX9 baseline and differential
GenevisibleiQ5VVX9 HS

Interactioni

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi127156, 71 interactors
IntActiQ5VVX9, 66 interactors
MINTiQ5VVX9
STRINGi9606.ENSP00000360116

Structurei

Secondary structure

1321
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5VVX9
SMRiQ5VVX9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5VVX9

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0419 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00900000140930
HOGENOMiHOG000049184
HOVERGENiHBG080217
InParanoidiQ5VVX9
KOiK10584
OMAiHPNVDPH
OrthoDBiEOG091G0ORT
PhylomeDBiQ5VVX9
TreeFamiTF329302

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5VVX9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MHGRAYLLLH RDFCDLKENN YKGITAKPVS EDMMEWEVEI EGLQNSVWQG
60 70 80 90 100
LVFQLTIHFT SEYNYAPPVV KFITIPFHPN VDPHTGQPCI DFLDNPEKWN
110 120 130 140 150
TNYTLSSILL ALQVMLSNPV LENPVNLEAA RILVKDESLY RTILRLFNRP
160 170 180 190 200
LQMKDDSQEL PKDPRKCIRP IKTTSFSDYY QTWSRIATSK ATEYYRTPLL
210 220 230 240 250
KVPNFIGQYY KWKKMDLQHQ KEWNLKYSVI KCWLARKRMP HEVTHSMEEI
260 270 280 290 300
KLCPTLIPTT DEIFLESPTA INSITDIYET EEEGWKSDTS LYENDTDEPR
310 320
EEEVEDLISW TNTLNTNTSE D
Length:321
Mass (Da):37,741
Last modified:December 7, 2004 - v1
Checksum:i8104FE91620417BB
GO
Isoform 2 (identifier: Q5VVX9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     227-321: Missing.

Show »
Length:226
Mass (Da):26,689
Checksum:i2DFF8434DD9452FB
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MRP4A0A0A0MRP4_HUMAN
Ubiquitin-conjugating enzyme E2 U
UBE2U
256Annotation score:
V9GZ31V9GZ31_HUMAN
Ubiquitin-conjugating enzyme E2 U
UBE2U
204Annotation score:
V9GYA6V9GYA6_HUMAN
Ubiquitin-conjugating enzyme E2 U
UBE2U
134Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05732390I → T. Corresponds to variant dbSNP:rs7532933Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016003227 – 321Missing in isoform 2. 1 PublicationAdd BLAST95

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445205 Genomic DNA No translation available.
AL161916 Genomic DNA No translation available.
BC029895 mRNA Translation: AAH29895.1
CCDSiCCDS627.1 [Q5VVX9-2]
RefSeqiNP_689702.1, NM_152489.2 [Q5VVX9-2]
UniGeneiHs.388746
Hs.656862

Genome annotation databases

EnsembliENST00000371076; ENSP00000360116; ENSG00000177414 [Q5VVX9-2]
ENST00000611228; ENSP00000481174; ENSG00000177414 [Q5VVX9-1]
GeneIDi148581
KEGGihsa:148581
UCSCiuc001dbn.1 human [Q5VVX9-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL445205 Genomic DNA No translation available.
AL161916 Genomic DNA No translation available.
BC029895 mRNA Translation: AAH29895.1
CCDSiCCDS627.1 [Q5VVX9-2]
RefSeqiNP_689702.1, NM_152489.2 [Q5VVX9-2]
UniGeneiHs.388746
Hs.656862

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1YRVX-ray2.18A1-150[»]
ProteinModelPortaliQ5VVX9
SMRiQ5VVX9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi127156, 71 interactors
IntActiQ5VVX9, 66 interactors
MINTiQ5VVX9
STRINGi9606.ENSP00000360116

Protein family/group databases

MoonDBiQ5VVX9 Predicted

PTM databases

iPTMnetiQ5VVX9
PhosphoSitePlusiQ5VVX9

Polymorphism and mutation databases

BioMutaiUBE2U
DMDMi74747311

Proteomic databases

PaxDbiQ5VVX9
PRIDEiQ5VVX9
ProteomicsDBi65500
65501 [Q5VVX9-2]

Protocols and materials databases

DNASUi148581
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371076; ENSP00000360116; ENSG00000177414 [Q5VVX9-2]
ENST00000611228; ENSP00000481174; ENSG00000177414 [Q5VVX9-1]
GeneIDi148581
KEGGihsa:148581
UCSCiuc001dbn.1 human [Q5VVX9-1]

Organism-specific databases

CTDi148581
EuPathDBiHostDB:ENSG00000177414.13
GeneCardsiUBE2U
HGNCiHGNC:28559 UBE2U
neXtProtiNX_Q5VVX9
OpenTargetsiENSG00000177414
PharmGKBiPA142670656
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0419 Eukaryota
COG5078 LUCA
GeneTreeiENSGT00900000140930
HOGENOMiHOG000049184
HOVERGENiHBG080217
InParanoidiQ5VVX9
KOiK10584
OMAiHPNVDPH
OrthoDBiEOG091G0ORT
PhylomeDBiQ5VVX9
TreeFamiTF329302

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkiQ5VVX9

Miscellaneous databases

EvolutionaryTraceiQ5VVX9
GenomeRNAii148581
PROiPR:Q5VVX9

Gene expression databases

BgeeiENSG00000177414 Expressed in 49 organ(s), highest expression level in right testis
CleanExiHS_UBE2U
ExpressionAtlasiQ5VVX9 baseline and differential
GenevisibleiQ5VVX9 HS

Family and domain databases

CDDicd00195 UBCc, 1 hit
Gene3Di3.10.110.10, 1 hit
InterProiView protein in InterPro
IPR000608 UBQ-conjugat_E2
IPR023313 UBQ-conjugating_AS
IPR016135 UBQ-conjugating_enzyme/RWD
PfamiView protein in Pfam
PF00179 UQ_con, 1 hit
SUPFAMiSSF54495 SSF54495, 1 hit
PROSITEiView protein in PROSITE
PS00183 UBIQUITIN_CONJUGAT_1, 1 hit
PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE2U_HUMAN
AccessioniPrimary (citable) accession number: Q5VVX9
Secondary accession number(s): Q8N1D4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: December 7, 2004
Last modified: September 12, 2018
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. SIMILARITY comments
    Index of protein domains and families
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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