UniProtKB - Q5VVX9 (UBE2U_HUMAN)
Protein
Ubiquitin-conjugating enzyme E2 U
Gene
UBE2U
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the covalent attachment of ubiquitin to other proteins.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation EC:2.3.2.23
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 89 | Glycyl thioester intermediatePROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ubiquitin conjugating enzyme activity Source: GO_Central
GO - Biological processi
- DNA repair Source: GO_Central
- histone ubiquitination Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- protein polyubiquitination Source: GO_Central
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
PathwayCommonsi | Q5VVX9 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q5VVX9 |
UniPathwayi | UPA00143 |
Protein family/group databases
MoonDBi | Q5VVX9, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-conjugating enzyme E2 U (EC:2.3.2.23)Alternative name(s): E2 ubiquitin-conjugating enzyme U Ubiquitin carrier protein U Ubiquitin-protein ligase U |
Gene namesi | Name:UBE2U |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:28559, UBE2U |
neXtProti | NX_Q5VVX9 |
VEuPathDBi | HostDB:ENSG00000177414.13 |
Subcellular locationi
Nucleus
- nuclear chromatin Source: GO_Central
Other locations
- HULC complex Source: GO_Central
Pathology & Biotechi
Organism-specific databases
DisGeNETi | 148581 |
OpenTargetsi | ENSG00000177414 |
PharmGKBi | PA142670656 |
Miscellaneous databases
Pharosi | Q5VVX9, Tdark |
Genetic variation databases
BioMutai | UBE2U |
DMDMi | 74747311 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082512 | 1 – 321 | Ubiquitin-conjugating enzyme E2 UAdd BLAST | 321 |
Post-translational modificationi
Autoubiquitinated in vitro in the presence of UBR5.1 Publication
Keywords - PTMi
Ubl conjugationProteomic databases
jPOSTi | Q5VVX9 |
PaxDbi | Q5VVX9 |
PRIDEi | Q5VVX9 |
ProteomicsDBi | 65500 [Q5VVX9-1] 65501 [Q5VVX9-2] |
PTM databases
iPTMneti | Q5VVX9 |
PhosphoSitePlusi | Q5VVX9 |
Expressioni
Gene expression databases
Bgeei | ENSG00000177414, Expressed in right testis and 75 other tissues |
ExpressionAtlasi | Q5VVX9, baseline and differential |
Genevisiblei | Q5VVX9, HS |
Organism-specific databases
HPAi | ENSG00000177414, Tissue enriched (testis) |
Interactioni
Binary interactionsi
Hide detailsQ5VVX9
With | #Exp. | IntAct |
---|---|---|
GOLGA2 [Q08379] | 4 | EBI-2130181,EBI-618309 |
TRIM32 [Q13049] | 4 | EBI-2130181,EBI-742790 |
Isoform 2 [Q5VVX9-2]
With | #Exp. | IntAct |
---|---|---|
GNB2 [P62879] | 3 | EBI-21897992,EBI-356942 |
VHL - isoform 2 [P40337-2] | 3 | EBI-21897992,EBI-12157263 |
Protein-protein interaction databases
BioGRIDi | 127156, 73 interactors |
IntActi | Q5VVX9, 70 interactors |
MINTi | Q5VVX9 |
STRINGi | 9606.ENSP00000360116 |
Miscellaneous databases
RNActi | Q5VVX9, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q5VVX9 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q5VVX9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 4 – 153 | UBC corePROSITE-ProRule annotationAdd BLAST | 150 |
Sequence similaritiesi
Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG0419, Eukaryota |
GeneTreei | ENSGT00940000162256 |
HOGENOMi | CLU_076373_0_0_1 |
InParanoidi | Q5VVX9 |
OMAi | HGLFFQL |
OrthoDBi | 1538096at2759 |
PhylomeDBi | Q5VVX9 |
TreeFami | TF329302 |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
IDEALi | IID00638 |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q5VVX9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MHGRAYLLLH RDFCDLKENN YKGITAKPVS EDMMEWEVEI EGLQNSVWQG
60 70 80 90 100
LVFQLTIHFT SEYNYAPPVV KFITIPFHPN VDPHTGQPCI DFLDNPEKWN
110 120 130 140 150
TNYTLSSILL ALQVMLSNPV LENPVNLEAA RILVKDESLY RTILRLFNRP
160 170 180 190 200
LQMKDDSQEL PKDPRKCIRP IKTTSFSDYY QTWSRIATSK ATEYYRTPLL
210 220 230 240 250
KVPNFIGQYY KWKKMDLQHQ KEWNLKYSVI KCWLARKRMP HEVTHSMEEI
260 270 280 290 300
KLCPTLIPTT DEIFLESPTA INSITDIYET EEEGWKSDTS LYENDTDEPR
310 320
EEEVEDLISW TNTLNTNTSE D
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0A0MRP4 | A0A0A0MRP4_HUMAN | Ubiquitin-conjugating enzyme E2 U | UBE2U | 256 | Annotation score: | ||
V9GZ31 | V9GZ31_HUMAN | Ubiquitin-conjugating enzyme E2 U | UBE2U | 204 | Annotation score: | ||
V9GYA6 | V9GYA6_HUMAN | Ubiquitin-conjugating enzyme E2 U | UBE2U | 134 | Annotation score: |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_057323 | 90 | I → T. Corresponds to variant dbSNP:rs7532933Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_016003 | 227 – 321 | Missing in isoform 2. 1 PublicationAdd BLAST | 95 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL445205 Genomic DNA No translation available. AL161916 Genomic DNA No translation available. BC029895 mRNA Translation: AAH29895.1 |
CCDSi | CCDS627.1 [Q5VVX9-2] |
RefSeqi | NP_689702.1, NM_152489.2 [Q5VVX9-2] |
Genome annotation databases
Ensembli | ENST00000371076; ENSP00000360116; ENSG00000177414 [Q5VVX9-2] ENST00000611228; ENSP00000481174; ENSG00000177414 [Q5VVX9-1] |
GeneIDi | 148581 |
KEGGi | hsa:148581 |
UCSCi | uc001dbn.1, human [Q5VVX9-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL445205 Genomic DNA No translation available. AL161916 Genomic DNA No translation available. BC029895 mRNA Translation: AAH29895.1 |
CCDSi | CCDS627.1 [Q5VVX9-2] |
RefSeqi | NP_689702.1, NM_152489.2 [Q5VVX9-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1YRV | X-ray | 2.18 | A | 1-150 | [»] | |
SMRi | Q5VVX9 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 127156, 73 interactors |
IntActi | Q5VVX9, 70 interactors |
MINTi | Q5VVX9 |
STRINGi | 9606.ENSP00000360116 |
Protein family/group databases
MoonDBi | Q5VVX9, Predicted |
PTM databases
iPTMneti | Q5VVX9 |
PhosphoSitePlusi | Q5VVX9 |
Genetic variation databases
BioMutai | UBE2U |
DMDMi | 74747311 |
Proteomic databases
jPOSTi | Q5VVX9 |
PaxDbi | Q5VVX9 |
PRIDEi | Q5VVX9 |
ProteomicsDBi | 65500 [Q5VVX9-1] 65501 [Q5VVX9-2] |
Protocols and materials databases
Antibodypediai | 19528, 148 antibodies |
DNASUi | 148581 |
Genome annotation databases
Ensembli | ENST00000371076; ENSP00000360116; ENSG00000177414 [Q5VVX9-2] ENST00000611228; ENSP00000481174; ENSG00000177414 [Q5VVX9-1] |
GeneIDi | 148581 |
KEGGi | hsa:148581 |
UCSCi | uc001dbn.1, human [Q5VVX9-1] |
Organism-specific databases
CTDi | 148581 |
DisGeNETi | 148581 |
GeneCardsi | UBE2U |
HGNCi | HGNC:28559, UBE2U |
HPAi | ENSG00000177414, Tissue enriched (testis) |
neXtProti | NX_Q5VVX9 |
OpenTargetsi | ENSG00000177414 |
PharmGKBi | PA142670656 |
VEuPathDBi | HostDB:ENSG00000177414.13 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0419, Eukaryota |
GeneTreei | ENSGT00940000162256 |
HOGENOMi | CLU_076373_0_0_1 |
InParanoidi | Q5VVX9 |
OMAi | HGLFFQL |
OrthoDBi | 1538096at2759 |
PhylomeDBi | Q5VVX9 |
TreeFami | TF329302 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q5VVX9 |
Reactomei | R-HSA-983168, Antigen processing: Ubiquitination & Proteasome degradation |
SignaLinki | Q5VVX9 |
Miscellaneous databases
BioGRID-ORCSi | 148581, 6 hits in 859 CRISPR screens |
EvolutionaryTracei | Q5VVX9 |
GenomeRNAii | 148581 |
Pharosi | Q5VVX9, Tdark |
PROi | PR:Q5VVX9 |
RNActi | Q5VVX9, protein |
Gene expression databases
Bgeei | ENSG00000177414, Expressed in right testis and 75 other tissues |
ExpressionAtlasi | Q5VVX9, baseline and differential |
Genevisiblei | Q5VVX9, HS |
Family and domain databases
CDDi | cd00195, UBCc, 1 hit |
Gene3Di | 3.10.110.10, 1 hit |
IDEALi | IID00638 |
InterProi | View protein in InterPro IPR000608, UBQ-conjugat_E2 IPR023313, UBQ-conjugating_AS IPR016135, UBQ-conjugating_enzyme/RWD |
Pfami | View protein in Pfam PF00179, UQ_con, 1 hit |
SUPFAMi | SSF54495, SSF54495, 1 hit |
PROSITEi | View protein in PROSITE PS00183, UBC_1, 1 hit PS50127, UBC_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBE2U_HUMAN | |
Accessioni | Q5VVX9Primary (citable) accession number: Q5VVX9 Secondary accession number(s): Q8N1D4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | December 7, 2004 | |
Last modified: | February 10, 2021 | |
This is version 147 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families