UBE2U

Functionⁱ

Catalyzes the covalent attachment of ubiquitin to other proteins.1 Publication

Catalytic activityⁱ

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Active siteⁱ	89	Glycyl thioester intermediatePROSITE-ProRule annotation		1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
ubiquitin protein ligase activity Source: GO_Central
ubiquitin protein ligase binding Source: GO_Central

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA repair Source: GO_Central
histone ubiquitination Source: GO_Central
proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
protein polyubiquitination Source: GO_Central

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Transferase
Biological process	Ubl conjugation pathway
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

Reactomeⁱ	R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkⁱ	Q5VVX9
UniPathwayⁱ	UPA00143

Protein family/group databases

MoonDBⁱ	Q5VVX9 Predicted

MoonDBⁱ

Q5VVX9 Predicted

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-conjugating enzyme E2 U (EC:2.3.2.23) Alternative name(s): E2 ubiquitin-conjugating enzyme U Ubiquitin carrier protein U Ubiquitin-protein ligase U
Gene namesⁱ	Name:UBE2U
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000177414.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:28559 UBE2U
neXtProtⁱ	NX_Q5VVX9

Subcellular locationⁱ

GO - Cellular component

Pathology & Biotechⁱ

Organism-specific databases

Open Targets More...OpenTargetsⁱ	ENSG00000177414
PharmGKBⁱ	PA142670656

Polymorphism and mutation databases

BioMutaⁱ	UBE2U
DMDMⁱ	74747311

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000082512	1 – 321	Ubiquitin-conjugating enzyme E2 UAdd BLAST		321

Post-translational modificationⁱ

Autoubiquitinated in vitro in the presence of UBR5.1 Publication

Keywords - PTMⁱ

Ubl conjugation

Proteomic databases

PaxDbⁱ	Q5VVX9
PRIDEⁱ	Q5VVX9
ProteomicsDBⁱ	65500 65501 [Q5VVX9-2]

PTM databases

iPTMnetⁱ	Q5VVX9
PhosphoSitePlusⁱ	Q5VVX9

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSG00000177414
CleanExⁱ	HS_UBE2U
ExpressionAtlasⁱ	Q5VVX9 baseline and differential
Genevisibleⁱ	Q5VVX9 HS

Interactionⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
GOLGA2	Q08379	4	EBI-2130181,EBI-618309
TRIM32	Q13049	4	EBI-2130181,EBI-742790

Show more details

GO - Molecular functionⁱ

ubiquitin protein ligase binding Source: GO_Central

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	127156, 71 interactors
IntActⁱ	Q5VVX9, 66 interactors
Molecular INTeraction database More...MINTⁱ	Q5VVX9
STRINGⁱ	9606.ENSP00000360116

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Show more details

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	4 – 19	Combined sources	16
Beta strandⁱ	24 – 28	Combined sources	5
Beta strandⁱ	35 – 41	Combined sources	7
Turnⁱ	47 – 50	Combined sources	4
Beta strandⁱ	52 – 58	Combined sources	7
Beta strandⁱ	69 – 74	Combined sources	6
Turnⁱ	83 – 85	Combined sources	3
Helixⁱ	91 – 94	Combined sources	4
Helixⁱ	96 – 98	Combined sources	3
Helixⁱ	105 – 116	Combined sources	12
Helixⁱ	127 – 134	Combined sources	8
Helixⁱ	137 – 147	Combined sources	11

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1YRV	X-ray	2.18	A	1-150	[»]
ProteinModelPortalⁱ	Q5VVX9
SMRⁱ	Q5VVX9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

Q5VVX9

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGⁱ	KOG0419 Eukaryota COG5078 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140930
HOGENOMⁱ	HOG000049184
HOVERGENⁱ	HBG080217
InParanoidⁱ	Q5VVX9
KEGG Orthology (KO) More...KOⁱ	K10584
OMAⁱ	HPNVDPH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0ORT
PhylomeDBⁱ	Q5VVX9
TreeFamⁱ	TF329302

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR000608 UBQ-conjugat_E2 IPR023313 UBQ-conjugating_AS IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS00183 UBIQUITIN_CONJUGAT_1, 1 hit PS50127 UBIQUITIN_CONJUGAT_2, 1 hit

Sequences (2)ⁱ

Sequence statusⁱ: Complete.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket Added to basket

Isoform 1 (identifier: Q5VVX9-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MHGRAYLLLH RDFCDLKENN YKGITAKPVS EDMMEWEVEI EGLQNSVWQG 
        60         70         80         90        100
LVFQLTIHFT SEYNYAPPVV KFITIPFHPN VDPHTGQPCI DFLDNPEKWN 
       110        120        130        140        150
TNYTLSSILL ALQVMLSNPV LENPVNLEAA RILVKDESLY RTILRLFNRP 
       160        170        180        190        200
LQMKDDSQEL PKDPRKCIRP IKTTSFSDYY QTWSRIATSK ATEYYRTPLL 
       210        220        230        240        250
KVPNFIGQYY KWKKMDLQHQ KEWNLKYSVI KCWLARKRMP HEVTHSMEEI 
       260        270        280        290        300
KLCPTLIPTT DEIFLESPTA INSITDIYET EEEGWKSDTS LYENDTDEPR 
       310        320 
EEEVEDLISW TNTLNTNTSE D

Length:321

Mass (Da):37,741

Last modified:December 7, 2004 - v1

Checksum:ⁱ8104FE91620417BB

GO

Isoform 2 (identifier: Q5VVX9-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
227-321: Missing.

« Hide

        10         20         30         40         50
MHGRAYLLLH RDFCDLKENN YKGITAKPVS EDMMEWEVEI EGLQNSVWQG 
        60         70         80         90        100
LVFQLTIHFT SEYNYAPPVV KFITIPFHPN VDPHTGQPCI DFLDNPEKWN 
       110        120        130        140        150
TNYTLSSILL ALQVMLSNPV LENPVNLEAA RILVKDESLY RTILRLFNRP 
       160        170        180        190        200
LQMKDDSQEL PKDPRKCIRP IKTTSFSDYY QTWSRIATSK ATEYYRTPLL 
       210        220 
KVPNFIGQYY KWKKMDLQHQ KEWNLK

Show »

Length:226

Mass (Da):26,689

Checksum:ⁱ2DFF8434DD9452FB

GO

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_057323	90	I → T. Corresponds to variant dbSNP:rs7532933Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_016003	227 – 321	Missing in isoform 2. 1 PublicationAdd BLAST		95

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL445205 Genomic DNA No translation available. AL161916 Genomic DNA No translation available. BC029895 mRNA Translation: AAH29895.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS627.1 [Q5VVX9-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_689702.1, NM_152489.2 [Q5VVX9-2]
UniGeneⁱ	Hs.388746 Hs.656862

Genome annotation databases

Ensemblⁱ	ENST00000371076; ENSP00000360116; ENSG00000177414 [Q5VVX9-2] ENST00000611228; ENSP00000481174; ENSG00000177414 [Q5VVX9-1]
GeneIDⁱ	148581
KEGGⁱ	hsa:148581
UCSC genome browser More...UCSCⁱ	uc001dbn.1 human [Q5VVX9-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Protein	Similar proteins		Species	Score	Length
Q5VVX9	Ubiquitin-conjugating enzyme E2 U (Fragment)		HUMAN		256
Ubiquitin-conjugating enzyme E2 U		MACFA		322
Ubiquitin conjugating enzyme E2 U (putative)		MACMU		226
UBE2U isoform 2		PONAB		322
Ubiquitin conjugating enzyme E2 U (putative)		CERAT		322
+45

Protein	Similar proteins		Species	Score	Length
Q5VVX9	Ubiquitin-conjugating enzyme E2 U (Fragment)		HUMAN		256
Ubiquitin conjugating enzyme E2 U (putative)		MACMU		322
UBE2U isoform 2		PONAB		322
UPI0005F4BDAD		MACNE		322
Uncharacterized protein		PANPA		322
+78

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AL445205 Genomic DNA No translation available. AL161916 Genomic DNA No translation available. BC029895 mRNA Translation: AAH29895.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS627.1 [Q5VVX9-2]
NCBI Reference Sequences More...RefSeqⁱ	NP_689702.1, NM_152489.2 [Q5VVX9-2]
UniGeneⁱ	Hs.388746 Hs.656862

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1YRV	X-ray	2.18	A	1-150	[»]
ProteinModelPortalⁱ	Q5VVX9
SMRⁱ	Q5VVX9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	127156, 71 interactors
IntActⁱ	Q5VVX9, 66 interactors
Molecular INTeraction database More...MINTⁱ	Q5VVX9
STRINGⁱ	9606.ENSP00000360116

Protein family/group databases

MoonDBⁱ	Q5VVX9 Predicted

MoonDBⁱ

Q5VVX9 Predicted

PTM databases

iPTMnetⁱ	Q5VVX9
PhosphoSitePlusⁱ	Q5VVX9

Polymorphism and mutation databases

BioMutaⁱ	UBE2U
DMDMⁱ	74747311

Proteomic databases

PaxDbⁱ	Q5VVX9
PRIDEⁱ	Q5VVX9
ProteomicsDBⁱ	65500 65501 [Q5VVX9-2]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	148581
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000371076; ENSP00000360116; ENSG00000177414 [Q5VVX9-2] ENST00000611228; ENSP00000481174; ENSG00000177414 [Q5VVX9-1]
GeneIDⁱ	148581
KEGGⁱ	hsa:148581
UCSC genome browser More...UCSCⁱ	uc001dbn.1 human [Q5VVX9-1]

Organism-specific databases

CTDⁱ	148581
EuPathDBⁱ	HostDB:ENSG00000177414.13
GeneCardsⁱ	UBE2U
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:28559 UBE2U
neXtProtⁱ	NX_Q5VVX9
Open Targets More...OpenTargetsⁱ	ENSG00000177414
PharmGKBⁱ	PA142670656
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0419 Eukaryota COG5078 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00900000140930
HOGENOMⁱ	HOG000049184
HOVERGENⁱ	HBG080217
InParanoidⁱ	Q5VVX9
KEGG Orthology (KO) More...KOⁱ	K10584
OMAⁱ	HPNVDPH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0ORT
PhylomeDBⁱ	Q5VVX9
TreeFamⁱ	TF329302

Enzyme and pathway databases

UniPathwayⁱ	UPA00143
Reactomeⁱ	R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
SignaLinkⁱ	Q5VVX9

Miscellaneous databases

EvolutionaryTraceⁱ	Q5VVX9
GenomeRNAiⁱ	148581
Protein Ontology More...PROⁱ	PR:Q5VVX9

Gene expression databases

Bgeeⁱ	ENSG00000177414
CleanExⁱ	HS_UBE2U
ExpressionAtlasⁱ	Q5VVX9 baseline and differential
Genevisibleⁱ	Q5VVX9 HS

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00195 UBCc, 1 hit
Gene3Dⁱ	3.10.110.10, 1 hit
InterProⁱ	View protein in InterPro IPR000608 UBQ-conjugat_E2 IPR023313 UBQ-conjugating_AS IPR016135 UBQ-conjugating_enzyme/RWD
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00179 UQ_con, 1 hit
SUPFAMⁱ	SSF54495 SSF54495, 1 hit
PROSITEⁱ	View protein in PROSITE PS00183 UBIQUITIN_CONJUGAT_1, 1 hit PS50127 UBIQUITIN_CONJUGAT_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	UBE2U_HUMAN
Accessionⁱ	Q5VVX9Primary (citable) accession number: Q5VVX9 Secondary accession number(s): Q8N1D4
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 25, 2005
	Last sequence update:	December 7, 2004
	Last modified:	June 20, 2018
	This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
PATHWAY comments
Index of metabolic and biosynthesis pathways
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

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UniRef

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Supporting data

UniProtKB - Q5VVX9 (UBE2U_HUMAN)

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Ubiquitin-conjugating enzyme E2 U

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ