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Protein

Cytosolic carboxypeptidase 6

Gene

AGBL4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes polyglutamates from the carboxy-terminus of target proteins such as MYLK. Mediates deglutamylation of CGAS, regulating the antiviral activity of CGAS. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5.By similarity

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi230ZincBy similarity1
Metal bindingi233ZincBy similarity1
Active sitei280NucleophileBy similarity1
Metal bindingi328ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-8955332 Carboxyterminal post-translational modifications of tubulin

Protein family/group databases

MEROPSiM14.027

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic carboxypeptidase 6 (EC:3.4.17.-)
Alternative name(s):
ATP/GTP-binding protein-like 4
Gene namesi
Name:AGBL4
Synonyms:CCP6
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000186094.16
HGNCiHGNC:25892 AGBL4
MIMi616476 gene
neXtProtiNX_Q5VU57

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Cytoskeleton, Golgi apparatus

Pathology & Biotechi

Organism-specific databases

DisGeNETi84871
OpenTargetsiENSG00000186094
PharmGKBiPA142672636

Polymorphism and mutation databases

BioMutaiAGBL4
DMDMi317373463

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002848351 – 503Cytosolic carboxypeptidase 6Add BLAST503

Proteomic databases

EPDiQ5VU57
PaxDbiQ5VU57
PeptideAtlasiQ5VU57
PRIDEiQ5VU57
ProteomicsDBi65385
65386 [Q5VU57-2]

PTM databases

iPTMnetiQ5VU57
PhosphoSitePlusiQ5VU57

Expressioni

Gene expression databases

BgeeiENSG00000186094 Expressed in 92 organ(s), highest expression level in amniotic fluid
CleanExiHS_AGBL4
ExpressionAtlasiQ5VU57 baseline and differential

Organism-specific databases

HPAiHPA037994

Interactioni

Subunit structurei

Interacts with MYLK.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9606.ENSP00000360905

Structurei

3D structure databases

ProteinModelPortaliQ5VU57
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M14 family.Curated

Phylogenomic databases

eggNOGiKOG3641 Eukaryota
COG2866 LUCA
GeneTreeiENSGT00550000074405
HOGENOMiHOG000231164
HOVERGENiHBG107588
InParanoidiQ5VU57
OMAiKWQRIPR
OrthoDBiEOG091G0FXK
PhylomeDBiQ5VU57
TreeFamiTF333009

Family and domain databases

InterProiView protein in InterPro
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5VU57-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAEGSQSAPE AGNDMGNDDA IGGNVSKYIV LPTGYCGQPK KGHLIFDACF
60 70 80 90 100
ESGNLGRVDQ VSEFEYDLFI RPDTCNPRFR VWFNFTVENV KESQRVIFNI
110 120 130 140 150
VNFSKTKSLY RDGMAPMVKS TSRPKWQRLP PKNVYYYRCP DHRKNYVMSF
160 170 180 190 200
AFCFDREEDI YQFAYCYPYT YTRFQHYLDS LQKRNMDYFF REQLGQSVQQ
210 220 230 240 250
RKLDLLTITS PDNLREGAEQ KVVFITGRVH PGETPSSFVC QGIIDFLVSQ
260 270 280 290 300
HPIACVLREY LVFKIAPMLN PDGVYLGNYR CSLMGFDLNR HWLDPSPWVH
310 320 330 340 350
PTLHGVKQLI VQMYNDPKTS LEFYIDIHAH STMMNGFMYG NIFEDEERFQ
360 370 380 390 400
RQAIFPKLLC QNAEDFSYSS TSFNRDAVKA GTGRRFLGGL LDHTSYCYTL
410 420 430 440 450
EVSFYSYIIS GTTAAVPYTE EAYMKLGRNV ARTFLDYYRL NPVVEKVAIP
460 470 480 490 500
MPRLRNKEIE VQRRKEKSPP YKHPLLRGPA SNYPNSKGDK KSSVNHKDPS

TPF
Length:503
Mass (Da):58,230
Last modified:January 11, 2011 - v3
Checksum:i4D1AD20229664D90
GO
Isoform 2 (identifier: Q5VU57-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     94-94: Q → QVREIVDTFRVVL
     455-463: Missing.

Note: No experimental confirmation available.
Show »
Length:506
Mass (Da):58,505
Checksum:i20C9AED5564F23F9
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AMW3B1AMW3_HUMAN
Cytosolic carboxypeptidase 6
AGBL4
320Annotation score:
H0Y5X4H0Y5X4_HUMAN
Cytosolic carboxypeptidase 6
AGBL4
299Annotation score:
F8VWL7F8VWL7_HUMAN
Cytosolic carboxypeptidase 6
AGBL4
292Annotation score:
B1ANV5B1ANV5_HUMAN
Cytosolic carboxypeptidase 6
AGBL4
217Annotation score:

Sequence cautioni

The sequence BAG52938 differs from that shown. Reason: Frameshift at position 501.Curated
The sequence BAG57648 differs from that shown. Reason: Frameshift at position 501.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti237S → L in BAG57648 (PubMed:14702039).Curated1
Sequence conflicti490K → R in BAG57648 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_061078443V → M. Corresponds to variant dbSNP:rs60977321Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04043594Q → QVREIVDTFRVVL in isoform 2. 1 Publication1
Alternative sequenceiVSP_040436455 – 463Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK094826 mRNA Translation: BAG52938.1 Frameshift.
AK294394 mRNA Translation: BAG57648.1 Frameshift.
AC099788 Genomic DNA No translation available.
AL355809 Genomic DNA No translation available.
AL390023 Genomic DNA No translation available.
AL391844 Genomic DNA No translation available.
AL590432 Genomic DNA No translation available.
AL591602 Genomic DNA No translation available.
AL645507 Genomic DNA No translation available.
AL645730 Genomic DNA No translation available.
CCDSiCCDS44137.1 [Q5VU57-1]
RefSeqiNP_001310502.1, NM_001323573.1 [Q5VU57-2]
NP_116174.3, NM_032785.3 [Q5VU57-1]
UniGeneiHs.551844
Hs.679809

Genome annotation databases

EnsembliENST00000371839; ENSP00000360905; ENSG00000186094 [Q5VU57-1]
GeneIDi84871
KEGGihsa:84871
UCSCiuc001cru.3 human [Q5VU57-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK094826 mRNA Translation: BAG52938.1 Frameshift.
AK294394 mRNA Translation: BAG57648.1 Frameshift.
AC099788 Genomic DNA No translation available.
AL355809 Genomic DNA No translation available.
AL390023 Genomic DNA No translation available.
AL391844 Genomic DNA No translation available.
AL590432 Genomic DNA No translation available.
AL591602 Genomic DNA No translation available.
AL645507 Genomic DNA No translation available.
AL645730 Genomic DNA No translation available.
CCDSiCCDS44137.1 [Q5VU57-1]
RefSeqiNP_001310502.1, NM_001323573.1 [Q5VU57-2]
NP_116174.3, NM_032785.3 [Q5VU57-1]
UniGeneiHs.551844
Hs.679809

3D structure databases

ProteinModelPortaliQ5VU57
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000360905

Protein family/group databases

MEROPSiM14.027

PTM databases

iPTMnetiQ5VU57
PhosphoSitePlusiQ5VU57

Polymorphism and mutation databases

BioMutaiAGBL4
DMDMi317373463

Proteomic databases

EPDiQ5VU57
PaxDbiQ5VU57
PeptideAtlasiQ5VU57
PRIDEiQ5VU57
ProteomicsDBi65385
65386 [Q5VU57-2]

Protocols and materials databases

DNASUi84871
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371839; ENSP00000360905; ENSG00000186094 [Q5VU57-1]
GeneIDi84871
KEGGihsa:84871
UCSCiuc001cru.3 human [Q5VU57-1]

Organism-specific databases

CTDi84871
DisGeNETi84871
EuPathDBiHostDB:ENSG00000186094.16
GeneCardsiAGBL4
HGNCiHGNC:25892 AGBL4
HPAiHPA037994
MIMi616476 gene
neXtProtiNX_Q5VU57
OpenTargetsiENSG00000186094
PharmGKBiPA142672636
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3641 Eukaryota
COG2866 LUCA
GeneTreeiENSGT00550000074405
HOGENOMiHOG000231164
HOVERGENiHBG107588
InParanoidiQ5VU57
OMAiKWQRIPR
OrthoDBiEOG091G0FXK
PhylomeDBiQ5VU57
TreeFamiTF333009

Enzyme and pathway databases

ReactomeiR-HSA-8955332 Carboxyterminal post-translational modifications of tubulin

Miscellaneous databases

ChiTaRSiAGBL4 human
GenomeRNAii84871
PROiPR:Q5VU57
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186094 Expressed in 92 organ(s), highest expression level in amniotic fluid
CleanExiHS_AGBL4
ExpressionAtlasiQ5VU57 baseline and differential

Family and domain databases

InterProiView protein in InterPro
IPR000834 Peptidase_M14
PfamiView protein in Pfam
PF00246 Peptidase_M14, 1 hit
SMARTiView protein in SMART
SM00631 Zn_pept, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCBPC6_HUMAN
AccessioniPrimary (citable) accession number: Q5VU57
Secondary accession number(s): B3KT26, B4DG37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 17, 2007
Last sequence update: January 11, 2011
Last modified: September 12, 2018
This is version 112 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Peptidase families
    Classification of peptidase families and list of entries
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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