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Protein

E3 ubiquitin-protein ligase BRE1A

Gene

RNF20

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation.4 Publications

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.3 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri922 – 961RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Transferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-8866654 E3 ubiquitin ligases ubiquitinate target proteins
SIGNORiQ5VTR2
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase BRE1A (EC:2.3.2.273 Publications)
Short name:
BRE1-A
Short name:
hBRE1
Alternative name(s):
RING finger protein 20
RING-type E3 ubiquitin transferase BRE1ACurated
Gene namesi
Name:RNF20
Synonyms:BRE1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000155827.11
HGNCiHGNC:10062 RNF20
MIMi607699 gene
neXtProtiNX_Q5VTR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi56254
OpenTargetsiENSG00000155827
PharmGKBiPA34427

Polymorphism and mutation databases

DMDMi84027766

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000558361 – 975E3 ubiquitin-protein ligase BRE1AAdd BLAST975

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21N6-acetyllysineBy similarity1
Modified residuei41PhosphoserineCombined sources1
Modified residuei136PhosphoserineCombined sources1
Modified residuei138PhosphoserineCombined sources1
Modified residuei348N6-acetyllysineCombined sources1
Modified residuei510N6-acetyllysineBy similarity1
Modified residuei522PhosphoserineCombined sources1
Modified residuei562PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ5VTR2
MaxQBiQ5VTR2
PaxDbiQ5VTR2
PeptideAtlasiQ5VTR2
PRIDEiQ5VTR2
ProteomicsDBi65346

PTM databases

iPTMnetiQ5VTR2
PhosphoSitePlusiQ5VTR2

Expressioni

Tissue specificityi

Expressed in the normal brain and also in malignant gliomas (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000155827 Expressed in 211 organ(s), highest expression level in deltoid
ExpressionAtlasiQ5VTR2 baseline and differential
GenevisibleiQ5VTR2 HS

Organism-specific databases

HPAiCAB012478
HPA051773

Interactioni

Subunit structurei

Component of the RNF20/40 complex (also known as BRE1 complex) probably composed of 2 copies of RNF20/BRE1A and 2 copies of RNF40/BRE1B. Interacts with UBE2E1/UBCH6. Interacts with p53/TP53 and WAC. Interacts with PAF1; the interaction mediates the association of the PAF1 and RNF20/40 complexes which is a prerequsite for recruitment of UBE2A/B. Interacts with isoform 1 and isoform 2 of PA2G4.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi121119, 92 interactors
CORUMiQ5VTR2
DIPiDIP-53411N
IntActiQ5VTR2, 43 interactors
MINTiQ5VTR2
STRINGi9606.ENSP00000373772

Structurei

Secondary structure

1975
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5VTR2
SMRiQ5VTR2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili43 – 90Sequence analysisAdd BLAST48
Coiled coili168 – 375Sequence analysisAdd BLAST208
Coiled coili429 – 898Sequence analysisAdd BLAST470

Sequence similaritiesi

Belongs to the BRE1 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri922 – 961RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG0978 Eukaryota
ENOG410Y5C6 LUCA
GeneTreeiENSGT00390000002866
HOVERGENiHBG080312
InParanoidiQ5VTR2
KOiK10696
OMAiNRYWNQF
OrthoDBiEOG091G01SA
PhylomeDBiQ5VTR2
TreeFamiTF323183

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR013956 E3_ubiquit_lig_Bre1
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PANTHERiPTHR23163 PTHR23163, 1 hit
SMARTiView protein in SMART
SM00184 RING, 1 hit
PROSITEiView protein in PROSITE
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

Q5VTR2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSGIGNKRAA GEPGTSMPPE KKAAVEDSGT TVETIKLGGV SSTEELDIRT
60 70 80 90 100
LQTKNRKLAE MLDQRQAIED ELREHIEKLE RRQATDDASL LIVNRYWSQF
110 120 130 140 150
DENIRIILKR YDLEQGLGDL LTERKALVVP EPEPDSDSNQ ERKDDRERGE
160 170 180 190 200
GQEPAFSFLA TLASSSSEEM ESQLQERVES SRRAVSQIVT VYDKLQEKVE
210 220 230 240 250
LLSRKLNSGD NLIVEEAVQE LNSFLAQENM RLQELTDLLQ EKHRTMSQEF
260 270 280 290 300
SKLQSKVETA ESRVSVLESM IDDLQWDIDK IRKREQRLNR HLAEVLERVN
310 320 330 340 350
SKGYKVYGAG SSLYGGTITI NARKFEEMNA ELEENKELAQ NRLCELEKLR
360 370 380 390 400
QDFEEVTTQN EKLKVELRSA VEQVVKETPE YRCMQSQFSV LYNESLQLKA
410 420 430 440 450
HLDEARTLLH GTRGTHQHQV ELIERDEVSL HKKLRTEVIQ LEDTLAQVRK
460 470 480 490 500
EYEMLRIEFE QTLAANEQAG PINREMRHLI SSLQNHNHQL KGEVLRYKRK
510 520 530 540 550
LREAQSDLNK TRLRSGSALL QSQSSTEDPK DEPAELKPDS EDLSSQSSAS
560 570 580 590 600
KASQEDANEI KSKRDEEERE RERREKERER EREREKEKER EREKQKLKES
610 620 630 640 650
EKERDSAKDK EKGKHDDGRK KEAEIIKQLK IELKKAQESQ KEMKLLLDMY
660 670 680 690 700
RSAPKEQRDK VQLMAAEKKS KAELEDLRQR LKDLEDKEKK ENKKMADEDA
710 720 730 740 750
LRKIRAVEEQ IEYLQKKLAM AKQEEEALLS EMDVTGQAFE DMQEQNIRLM
760 770 780 790 800
QQLREKDDAN FKLMSERIKS NQIHKLLKEE KEELADQVLT LKTQVDAQLQ
810 820 830 840 850
VVRKLEEKEH LLQSNIGTGE KELGLRTQAL EMNKRKAMEA AQLADDLKAQ
860 870 880 890 900
LELAQKKLHD FQDEIVENSV TKEKDMFNFK RAQEDISRLR RKLETTKKPD
910 920 930 940 950
NVPKCDEILM EEIKDYKARL TCPCCNMRKK DAVLTKCFHV FCFECVKTRY
960 970
DTRQRKCPKC NAAFGANDFH RIYIG
Length:975
Mass (Da):113,662
Last modified:December 20, 2005 - v2
Checksum:iD75C7BE02880594A
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9JXC9C9JXC9_HUMAN
E3 ubiquitin protein ligase
RNF20
181Annotation score:
C9J0A5C9J0A5_HUMAN
E3 ubiquitin protein ligase
RNF20
145Annotation score:
C9JWJ4C9JWJ4_HUMAN
E3 ubiquitin protein ligase
RNF20
99Annotation score:

Sequence cautioni

The sequence AAH63115 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA91326 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAA92057 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAB14005 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149G → GKW in CAH69963 (PubMed:15164053).Curated1
Sequence conflicti149G → GKW in CAI14618 (PubMed:15164053).Curated1
Sequence conflicti285E → Q in BAA91134 (PubMed:14702039).Curated1
Sequence conflicti285E → Q in BAA91326 (PubMed:14702039).Curated1
Sequence conflicti289N → D in AAK58539 (Ref. 1) Curated1
Sequence conflicti322A → V in BAB14081 (PubMed:14702039).Curated1
Sequence conflicti668K → E in BAB14081 (PubMed:14702039).Curated1
Sequence conflicti693K → T in AAK58539 (Ref. 1) Curated1
Sequence conflicti699D → E in BAB14081 (PubMed:14702039).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF265230 mRNA Translation: AAK58539.1
AK000389 mRNA Translation: BAA91134.1 Sequence problems.
AK000697 mRNA Translation: BAA91326.1 Different initiation.
AK002051 mRNA Translation: BAA92057.1 Different initiation.
AK022300 mRNA Translation: BAB14005.1 Different initiation.
AK022532 mRNA Translation: BAB14081.1
AK314401 mRNA Translation: BAG37025.1
AL591377, AL353621 Genomic DNA Translation: CAH69963.1
AL353621, AL591377 Genomic DNA Translation: CAI14618.1
BC063115 mRNA Translation: AAH63115.1 Sequence problems.
BC110584 mRNA Translation: AAI10585.1
BC110585 mRNA Translation: AAI10586.1
BC152309 mRNA Translation: AAI52310.1
AL832910 mRNA Translation: CAH10630.1
AL834272 mRNA Translation: CAD38947.1
CCDSiCCDS35084.1
RefSeqiNP_062538.5, NM_019592.6
XP_011517164.1, XM_011518862.1
UniGeneiHs.729085

Genome annotation databases

EnsembliENST00000389120; ENSP00000373772; ENSG00000155827
GeneIDi56254
KEGGihsa:56254
UCSCiuc004bbn.5 human

Similar proteinsi

Entry informationi

Entry nameiBRE1A_HUMAN
AccessioniPrimary (citable) accession number: Q5VTR2
Secondary accession number(s): A7MCT5
, Q2TB34, Q69YL5, Q6P527, Q8N3J4, Q96JD3, Q9H9Y7, Q9HA51, Q9NUR4, Q9NWQ3, Q9NX83
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: September 12, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health

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