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Protein

E3 ubiquitin-protein ligase RNF220

Gene

RNF220

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that promotes the ubiquitination and proteasomal degradation of SIN3B (By similarity). Independently of its E3 ligase activity, acts as a CTNNB1 stabilizer through USP7-mediated deubiquitination of CTNNB1 promoting Wnt signaling (PubMed:25266658).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri514 – 553RING-typePROSITE-ProRule annotationAdd BLAST40

GO - Molecular functioni

GO - Biological processi

  • positive regulation of canonical Wnt signaling pathway Source: UniProtKB
  • protein autoubiquitination Source: UniProtKB
  • protein ubiquitination Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF220 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 220
RING-type E3 ubiquitin transferase RNF220Curated
Gene namesi
Name:RNF220
Synonyms:C1orf164
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000187147.17
HGNCiHGNC:25552 RNF220
MIMi616136 gene
neXtProtiNX_Q5VTB9

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

DisGeNETi55182
OpenTargetsiENSG00000187147
PharmGKBiPA162401886

Polymorphism and mutation databases

BioMutaiRNF220
DMDMi74756788

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002776571 – 566E3 ubiquitin-protein ligase RNF220Add BLAST566

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki277Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei390PhosphoserineCombined sources1

Post-translational modificationi

Auto-ubiquitinated; leads to proteasomal degradation.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5VTB9
MaxQBiQ5VTB9
PaxDbiQ5VTB9
PeptideAtlasiQ5VTB9
PRIDEiQ5VTB9
ProteomicsDBi65319

PTM databases

iPTMnetiQ5VTB9
PhosphoSitePlusiQ5VTB9

Expressioni

Gene expression databases

BgeeiENSG00000187147 Expressed in 225 organ(s), highest expression level in corpus callosum
CleanExiHS_RNF220
ExpressionAtlasiQ5VTB9 baseline and differential
GenevisibleiQ5VTB9 HS

Organism-specific databases

HPAiHPA027578

Interactioni

Subunit structurei

Interacts with SIN3B (By similarity). Interacts with CTNNB1 (via Armadillo repeats 2-8) (PubMed:25266658). Interacts with USP7 (via MATH domain) (PubMed:25266658).By similarity1 Publication

Protein-protein interaction databases

BioGridi120481, 17 interactors
IntActiQ5VTB9, 7 interactors
STRINGi9606.ENSP00000347548

Structurei

3D structure databases

ProteinModelPortaliQ5VTB9
SMRiQ5VTB9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni514 – 522Required for targeting to the cytoplasmBy similarity9

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili485 – 513Sequence analysisAdd BLAST29

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri514 – 553RING-typePROSITE-ProRule annotationAdd BLAST40

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IEJC Eukaryota
ENOG4111JY9 LUCA
GeneTreeiENSGT00390000016573
HOGENOMiHOG000231748
HOVERGENiHBG055526
InParanoidiQ5VTB9
OMAiXGAKKLC
OrthoDBiEOG091G0PN3
PhylomeDBiQ5VTB9
TreeFamiTF324716

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031824 RNF220_mid
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF15926 RNF220, 2 hits
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5VTB9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MDLHRAAFKM ENSSYLPNPL ASPALMVLAS TAEASRDASI PCQQPRPFGV
60 70 80 90 100
PVSVDKDVHI PFTNGSYTFA SMYHRQGGVP GTFANRDFPP SLLHLHPQFA
110 120 130 140 150
PPNLDCTPIS MLNHSGVGAF RPFASTEDRE SYQSAFTPAK RLKNCHDTES
160 170 180 190 200
PHLRFSDADG KEYDFGTQLP SSSPGSLKVD DTGKKIFAVS GLISDREASS
210 220 230 240 250
SPEDRNDRCK KKAAALFDSQ APICPICQVL LRPSELQEHM EQELEQLAQL
260 270 280 290 300
PSSKNSLLKD AMAPGTPKSL LLSASIKREG ESPTASPHSS ATDDLHHSDR
310 320 330 340 350
YQTFLRVRAN RQTRLNARIG KMKRRKQDEG QREGSCMAED DAVDIEHENN
360 370 380 390 400
NRFEEYEWCG QKRIRATTLL EGGFRGSGFI MCSGKENPDS DADLDVDGDD
410 420 430 440 450
TLEYGKPQYT EADVIPCTGE EPGEAKEREA LRGAVLNGGP PSTRITPEFS
460 470 480 490 500
KWASDEMPST SNGESSKQEA MQKTCKNSDI EKITEDSAVT TFEALKARVR
510 520 530 540 550
ELERQLSRGD RYKCLICMDS YSMPLTSIQC WHVHCEECWL RTLGAKKLCP
560
QCNTITAPGD LRRIYL
Length:566
Mass (Da):62,765
Last modified:December 7, 2004 - v1
Checksum:iAAE3C723032B062B
GO
Isoform 2 (identifier: Q5VTB9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-239: Missing.
     331-331: Q → QVCPLCNRPLAGSEQEMSRHVEHCLSK

Note: No experimental confirmation available.
Show »
Length:353
Mass (Da):39,570
Checksum:i9014236EFCF08F29
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5TDE7Q5TDE7_HUMAN
E3 ubiquitin-protein ligase RNF220
RNF220
259Annotation score:
Q5TDE8Q5TDE8_HUMAN
E3 ubiquitin-protein ligase RNF220
RNF220
260Annotation score:
H0Y7B1H0Y7B1_HUMAN
E3 ubiquitin-protein ligase RNF220
RNF220
121Annotation score:
U3KPZ6U3KPZ6_HUMAN
E3 ubiquitin-protein ligase RNF220
RNF220
45Annotation score:

Sequence cautioni

The sequence BAA91704 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84A → V in AAH98300 (PubMed:15489334).Curated1
Sequence conflicti277K → E in BAG51705 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0554371 – 239Missing in isoform 2. 1 PublicationAdd BLAST239
Alternative sequenceiVSP_055438331Q → QVCPLCNRPLAGSEQEMSRH VEHCLSK in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001459 mRNA Translation: BAA91704.1 Different initiation.
AK056424 mRNA Translation: BAG51705.1
AK297228 mRNA Translation: BAG59711.1
AL122004 Genomic DNA No translation available.
AL359373 Genomic DNA No translation available.
AL596225 Genomic DNA No translation available.
BC034221 mRNA Translation: AAH34221.1
BC098266 mRNA Translation: AAH98266.1
BC098300 mRNA Translation: AAH98300.1
CCDSiCCDS510.1 [Q5VTB9-1]
RefSeqiNP_001306885.1, NM_001319956.1 [Q5VTB9-1]
NP_001306886.1, NM_001319957.1 [Q5VTB9-3]
NP_060620.2, NM_018150.3 [Q5VTB9-1]
XP_016857112.1, XM_017001623.1 [Q5VTB9-1]
XP_016857113.1, XM_017001624.1 [Q5VTB9-1]
XP_016857114.1, XM_017001625.1 [Q5VTB9-1]
UniGeneiHs.456557

Genome annotation databases

EnsembliENST00000355387; ENSP00000347548; ENSG00000187147 [Q5VTB9-1]
ENST00000361799; ENSP00000354872; ENSG00000187147 [Q5VTB9-1]
ENST00000372247; ENSP00000361321; ENSG00000187147 [Q5VTB9-1]
GeneIDi55182
KEGGihsa:55182
UCSCiuc001clv.2 human [Q5VTB9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK001459 mRNA Translation: BAA91704.1 Different initiation.
AK056424 mRNA Translation: BAG51705.1
AK297228 mRNA Translation: BAG59711.1
AL122004 Genomic DNA No translation available.
AL359373 Genomic DNA No translation available.
AL596225 Genomic DNA No translation available.
BC034221 mRNA Translation: AAH34221.1
BC098266 mRNA Translation: AAH98266.1
BC098300 mRNA Translation: AAH98300.1
CCDSiCCDS510.1 [Q5VTB9-1]
RefSeqiNP_001306885.1, NM_001319956.1 [Q5VTB9-1]
NP_001306886.1, NM_001319957.1 [Q5VTB9-3]
NP_060620.2, NM_018150.3 [Q5VTB9-1]
XP_016857112.1, XM_017001623.1 [Q5VTB9-1]
XP_016857113.1, XM_017001624.1 [Q5VTB9-1]
XP_016857114.1, XM_017001625.1 [Q5VTB9-1]
UniGeneiHs.456557

3D structure databases

ProteinModelPortaliQ5VTB9
SMRiQ5VTB9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120481, 17 interactors
IntActiQ5VTB9, 7 interactors
STRINGi9606.ENSP00000347548

PTM databases

iPTMnetiQ5VTB9
PhosphoSitePlusiQ5VTB9

Polymorphism and mutation databases

BioMutaiRNF220
DMDMi74756788

Proteomic databases

EPDiQ5VTB9
MaxQBiQ5VTB9
PaxDbiQ5VTB9
PeptideAtlasiQ5VTB9
PRIDEiQ5VTB9
ProteomicsDBi65319

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000355387; ENSP00000347548; ENSG00000187147 [Q5VTB9-1]
ENST00000361799; ENSP00000354872; ENSG00000187147 [Q5VTB9-1]
ENST00000372247; ENSP00000361321; ENSG00000187147 [Q5VTB9-1]
GeneIDi55182
KEGGihsa:55182
UCSCiuc001clv.2 human [Q5VTB9-1]

Organism-specific databases

CTDi55182
DisGeNETi55182
EuPathDBiHostDB:ENSG00000187147.17
GeneCardsiRNF220
H-InvDBiHIX0000517
HGNCiHGNC:25552 RNF220
HPAiHPA027578
MIMi616136 gene
neXtProtiNX_Q5VTB9
OpenTargetsiENSG00000187147
PharmGKBiPA162401886
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IEJC Eukaryota
ENOG4111JY9 LUCA
GeneTreeiENSGT00390000016573
HOGENOMiHOG000231748
HOVERGENiHBG055526
InParanoidiQ5VTB9
OMAiXGAKKLC
OrthoDBiEOG091G0PN3
PhylomeDBiQ5VTB9
TreeFamiTF324716

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

ChiTaRSiRNF220 human
GeneWikiiRNF220
GenomeRNAii55182
PROiPR:Q5VTB9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000187147 Expressed in 225 organ(s), highest expression level in corpus callosum
CleanExiHS_RNF220
ExpressionAtlasiQ5VTB9 baseline and differential
GenevisibleiQ5VTB9 HS

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR031824 RNF220_mid
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF15926 RNF220, 2 hits
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRN220_HUMAN
AccessioniPrimary (citable) accession number: Q5VTB9
Secondary accession number(s): B3KPJ3
, B4DLZ9, E9PCS1, Q4KMX2, Q9NVP6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 20, 2007
Last sequence update: December 7, 2004
Last modified: October 10, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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