UniProtKB - Q5U4D9 (THOC6_MOUSE)
Protein
THO complex subunit 6 homolog
Gene
Thoc6
Organism
Mus musculus (Mouse)
Status
Functioni
Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway.Plays a role in apoptosis negative control involved in brain development (By similarity).By similarity
GO - Molecular functioni
- RNA binding Source: UniProtKB-KW
GO - Biological processi
- apoptotic process Source: UniProtKB-KW
- central nervous system development Source: UniProtKB
- mRNA export from nucleus Source: MGI
- mRNA processing Source: UniProtKB-KW
- negative regulation of apoptotic process Source: UniProtKB
- RNA splicing Source: UniProtKB-KW
- viral mRNA export from host cell nucleus Source: MGI
Keywordsi
Molecular function | Developmental protein, RNA-binding |
Biological process | Apoptosis, mRNA processing, mRNA splicing, mRNA transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-MMU-72187, mRNA 3'-end processing R-MMU-73856, RNA Polymerase II Transcription Termination |
Names & Taxonomyi
Protein namesi | Recommended name: THO complex subunit 6 homologAlternative name(s): WD repeat-containing protein 58 |
Gene namesi | Name:Thoc6 Synonyms:Wdr58 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2677480, Thoc6 |
Subcellular locationi
Nucleus
- Nucleus By similarity
- Nucleus speckle By similarity
Nucleus
- nuclear body Source: MGI
- nuclear chromosome, telomeric region Source: Ensembl
- nuclear speck Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
- THO complex Source: MGI
- THO complex part of transcription export complex Source: MGI
- transcription export complex Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000233159 | 1 – 341 | THO complex subunit 6 homologAdd BLAST | 341 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 180 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q5U4D9 |
MaxQBi | Q5U4D9 |
PaxDbi | Q5U4D9 |
PeptideAtlasi | Q5U4D9 |
PRIDEi | Q5U4D9 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00123949 |
PTM databases
iPTMneti | Q5U4D9 |
PhosphoSitePlusi | Q5U4D9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000041319, Expressed in epiblast (generic) and 234 other tissues |
ExpressionAtlasi | Q5U4D9, baseline and differential |
Genevisiblei | Q5U4D9, MM |
Interactioni
Subunit structurei
Component of the THO complex, which is composed of THOC1, THOC2, THOC3, THOC5, THOC6 and THOC7; together with at least ALYREF/THOC4, DDX39B, SARNP/CIP29 and CHTOP, THO forms the transcription/export (TREX) complex which seems to have a dynamic structure involving ATP-dependent remodeling.
By similarityProtein-protein interaction databases
BioGRIDi | 239749, 2 interactors |
IntActi | Q5U4D9, 2 interactors |
STRINGi | 10090.ENSMUSP00000038137 |
Miscellaneous databases
RNActi | Q5U4D9, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 22 – 61 | WD 1Add BLAST | 40 | |
Repeati | 74 – 112 | WD 2Add BLAST | 39 | |
Repeati | 124 – 165 | WD 3Add BLAST | 42 | |
Repeati | 166 – 205 | WD 4Add BLAST | 40 | |
Repeati | 215 – 254 | WD 5Add BLAST | 40 | |
Repeati | 256 – 293 | WD 6Add BLAST | 38 | |
Repeati | 295 – 339 | WD 7Add BLAST | 45 |
Sequence similaritiesi
Belongs to the WD repeat THOC6 family.Curated
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | KOG0649, Eukaryota |
GeneTreei | ENSGT00390000015278 |
HOGENOMi | CLU_060667_0_0_1 |
InParanoidi | Q5U4D9 |
OrthoDBi | 760323at2759 |
PhylomeDBi | Q5U4D9 |
TreeFami | TF324760 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR042626, THOC6 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR44411, PTHR44411, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q5U4D9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEHAAPLAVP LGQAEVFQAL QRLHMTIFSQ SVSPCGKFLA AGNNYGQIAI
60 70 80 90 100
FSLSAALSSE AKEESKKPVV VFHAHDGPVY SMVSTDRHLL SAGDGEVKGW
110 120 130 140 150
LWAEILKKGC KELWRRQPPY RTSLEVPEIN ALLLVPKENS LILAGGDCQL
160 170 180 190 200
HSMDLETGAF TRALRGHTDY IHCLALRERS PEVLSGGEDG AVRLWDLRIA
210 220 230 240 250
KEVQTIEVYK HEECSRPHNG RWIGCLATDS DWMVCGGGPA LTLWHLRSST
260 270 280 290 300
PTTVFPIRAP QKHVTFYQDL ILSAGQGCCV NHWQLSGELK AQVPGSSPGL
310 320 330 340
LSLSLNQQPA APECKVLTAS GNSCRVDVFT NLGYRAFSLS F
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD3Z132 | D3Z132_MOUSE | THO complex subunit 6 homolog | Thoc6 | 337 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC085131 mRNA Translation: AAH85131.1 BC089551 mRNA Translation: AAH89551.1 |
CCDSi | CCDS28454.1 |
RefSeqi | NP_001008425.1, NM_001008425.1 XP_006524607.1, XM_006524544.2 |
Genome annotation databases
Ensembli | ENSMUST00000047436; ENSMUSP00000038137; ENSMUSG00000041319 ENSMUST00000095579; ENSMUSP00000093239; ENSMUSG00000041319 ENSMUST00000115490; ENSMUSP00000111153; ENSMUSG00000041319 |
GeneIDi | 386612 |
KEGGi | mmu:386612 |
UCSCi | uc008ass.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC085131 mRNA Translation: AAH85131.1 BC089551 mRNA Translation: AAH89551.1 |
CCDSi | CCDS28454.1 |
RefSeqi | NP_001008425.1, NM_001008425.1 XP_006524607.1, XM_006524544.2 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 239749, 2 interactors |
IntActi | Q5U4D9, 2 interactors |
STRINGi | 10090.ENSMUSP00000038137 |
PTM databases
iPTMneti | Q5U4D9 |
PhosphoSitePlusi | Q5U4D9 |
2D gel databases
REPRODUCTION-2DPAGEi | IPI00123949 |
Proteomic databases
EPDi | Q5U4D9 |
MaxQBi | Q5U4D9 |
PaxDbi | Q5U4D9 |
PeptideAtlasi | Q5U4D9 |
PRIDEi | Q5U4D9 |
Protocols and materials databases
Antibodypediai | 23992, 155 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000047436; ENSMUSP00000038137; ENSMUSG00000041319 ENSMUST00000095579; ENSMUSP00000093239; ENSMUSG00000041319 ENSMUST00000115490; ENSMUSP00000111153; ENSMUSG00000041319 |
GeneIDi | 386612 |
KEGGi | mmu:386612 |
UCSCi | uc008ass.1, mouse |
Organism-specific databases
CTDi | 79228 |
MGIi | MGI:2677480, Thoc6 |
Phylogenomic databases
eggNOGi | KOG0649, Eukaryota |
GeneTreei | ENSGT00390000015278 |
HOGENOMi | CLU_060667_0_0_1 |
InParanoidi | Q5U4D9 |
OrthoDBi | 760323at2759 |
PhylomeDBi | Q5U4D9 |
TreeFami | TF324760 |
Enzyme and pathway databases
Reactomei | R-MMU-159236, Transport of Mature mRNA derived from an Intron-Containing Transcript R-MMU-72187, mRNA 3'-end processing R-MMU-73856, RNA Polymerase II Transcription Termination |
Miscellaneous databases
BioGRID-ORCSi | 386612, 5 hits in 18 CRISPR screens |
ChiTaRSi | Thoc6, mouse |
PROi | PR:Q5U4D9 |
RNActi | Q5U4D9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000041319, Expressed in epiblast (generic) and 234 other tissues |
ExpressionAtlasi | Q5U4D9, baseline and differential |
Genevisiblei | Q5U4D9, MM |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
InterProi | View protein in InterPro IPR042626, THOC6 IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR017986, WD40_repeat_dom IPR036322, WD40_repeat_dom_sf |
PANTHERi | PTHR44411, PTHR44411, 1 hit |
Pfami | View protein in Pfam PF00400, WD40, 1 hit |
SMARTi | View protein in SMART SM00320, WD40, 3 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | THOC6_MOUSE | |
Accessioni | Q5U4D9Primary (citable) accession number: Q5U4D9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 2, 2006 |
Last sequence update: | December 7, 2004 | |
Last modified: | December 2, 2020 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families