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Entry version 103 (12 Aug 2020)
Sequence version 1 (07 Dec 2004)
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Protein

Sperm-associated antigen 1

Gene

Spag1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

May play a role in the cytoplasmic assembly of the ciliary dynein arms (By similarity). Binds GTP and has GTPase activity (By similarity). Plays a role in fertilization.By similarity1 Publication

Miscellaneous

Antibodies against SPAG1 interfere with fertilization.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi630 – 637GTPSequence analysis8

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase
Biological processFertilization
LigandGTP-binding, Nucleotide-binding

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Sperm-associated antigen 1
Alternative name(s):
HSD-3.8
Infertility-related sperm protein Spag-1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Spag1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiRattus norvegicus (Rat)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10116 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002494 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 7

Organism-specific databases

Rat genome database

More...
RGDi
1310702, Spag1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001063261 – 893Sperm-associated antigen 1Add BLAST893

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei351PhosphoserineCombined sources1
Modified residuei703PhosphoserineCombined sources1
Modified residuei739PhosphoserineCombined sources1
Modified residuei740PhosphoserineCombined sources1
Modified residuei758PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
Q5U2X2

PRoteomics IDEntifications database

More...
PRIDEi
Q5U2X2

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
Q5U2X2

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...
PhosphoSitePlusi
Q5U2X2

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Testis and sperm.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...
Bgeei
ENSRNOG00000010078, Expressed in testis and 20 other tissues

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

STRING: functional protein association networks

More...
STRINGi
10116.ENSRNOP00000013801

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
Q5U2X2

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section indicates the positions and types of repeated sequence motifs or repeated domains within the protein.<p><a href='/help/repeat' target='_top'>More...</a></p>Repeati213 – 246TPR 1Add BLAST34
Repeati247 – 279TPR 2Add BLAST33
Repeati280 – 313TPR 3Add BLAST34
Repeati429 – 463TPR 4Add BLAST35
Repeati471 – 504TPR 5Add BLAST34
Repeati506 – 538TPR 6Add BLAST33
Repeati605 – 638TPR 7Add BLAST34
Repeati639 – 672TPR 8Add BLAST34

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG1124, Eukaryota

Ensembl GeneTree

More...
GeneTreei
ENSGT00940000154697

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_008405_1_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
Q5U2X2

KEGG Orthology (KO)

More...
KOi
K19870

Identification of Orthologs from Complete Genome Data

More...
OMAi
QYRKAYV

Database of Orthologous Groups

More...
OrthoDBi
1070087at2759

Database for complete collections of gene phylogenies

More...
PhylomeDBi
Q5U2X2

TreeFam database of animal gene trees

More...
TreeFami
TF106251

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
1.25.40.10, 3 hits

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR025986, RPAP3-like_C
IPR013026, TPR-contain_dom
IPR011990, TPR-like_helical_dom_sf
IPR001440, TPR_1
IPR019734, TPR_repeat

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF13877, RPAP3_C, 1 hit
PF00515, TPR_1, 2 hits
PF13181, TPR_8, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00028, TPR, 8 hits

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF48452, SSF48452, 3 hits

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS50005, TPR, 5 hits
PS50293, TPR_REGION, 4 hits

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

Q5U2X2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTAKVKDHPP LWGFGTTKTF KIPIEHLDFK YIENCSDVKQ LEKILCVLRS
60 70 80 90 100
GEEGYYPELT EFCEKRLTGL APRSRALRKD KPAATASSFS AEEWEKIDSD
110 120 130 140 150
LKSWVSEIKR EENTRHFHDP EKHPGVEDPL PPVRGSNSCP RGGKETSSKS
160 170 180 190 200
KTAKKRIPRD YAEWDKFDVE KECSKIDEDY KEKTVINNKA HLSKIETKID
210 220 230 240 250
TAGLTEKEKN FLANREKGKG NEAFYSGDYE EAVMYYTRSL SALPTATAYN
260 270 280 290 300
NRAQAEIKLQ RWSSALEDCE KALELEPGNI KALLRRATTY KHQNKFLEAV
310 320 330 340 350
DDLRKVLQAE PDNDLAKKTL SEVERELKNS EPASELQTKG KRMVIEEVEN
360 370 380 390 400
SGDEGGKGDE DDHEDDGVDM AAMGNIQKKL MVRSQGGRRS RRARTPMPGA
410 420 430 440 450
EQQEGQPETG TASTSDNHDL EERRAADSPG DLKSRGNELF RGGQFAEAAV
460 470 480 490 500
QYSGAIAQLE PTGSENADEL SILYSNRAAC YLKEGNCRGC IQDCDRALEL
510 520 530 540 550
QPFAVKPLLR RAMAYETLEQ YRSAYVDYIT VLKIDCRIQL ASDSVNRITR
560 570 580 590 600
ILTELDGPKW RERLPPIPAV PASEPLRVWH PAAETPDQDP CPNSCTPTIT
610 620 630 640 650
DEKMFQALKE EGNQLVKDKN YKDAISKYNE CLKINSKACA IYTNRALCYL
660 670 680 690 700
KLGQFEEAKL DCDKALQIDS KNVKASYRLE LAQKGLENCR ERVADPSQVV
710 720 730 740 750
LLSPDSSEAA RHLDTKNDTA PPSRERERRR IEIQEVDDSS DEEPERPAEA
760 770 780 790 800
SAVEEGWSAE RAGKIEVCKP RNAYEFGQVL STISARKDEE ACAQLLVFTA
810 820 830 840 850
PQDLPVLLSN KLEGDMLLLI MQSLKSHLVA KDPSLVCKHL LYLSKAERFE
860 870 880 890
MMLALTSKDQ KEQMAQLFDD LSDAQADCLT AEDIQALRRQ YVL
Length:893
Mass (Da):100,615
Last modified:December 7, 2004 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iE95507EFF0B3A49F
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
BC085828 mRNA Translation: AAH85828.1

NCBI Reference Sequences

More...
RefSeqi
NP_001012116.1, NM_001012116.1

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENSRNOT00000013801; ENSRNOP00000013801; ENSRNOG00000010078

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
315033

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
rno:315033

UCSC genome browser

More...
UCSCi
RGD:1310702, rat

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085828 mRNA Translation: AAH85828.1
RefSeqiNP_001012116.1, NM_001012116.1

3D structure databases

SMRiQ5U2X2
ModBaseiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013801

PTM databases

iPTMnetiQ5U2X2
PhosphoSitePlusiQ5U2X2

Proteomic databases

PaxDbiQ5U2X2
PRIDEiQ5U2X2

Genome annotation databases

EnsembliENSRNOT00000013801; ENSRNOP00000013801; ENSRNOG00000010078
GeneIDi315033
KEGGirno:315033
UCSCiRGD:1310702, rat

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
6674
RGDi1310702, Spag1

Phylogenomic databases

eggNOGiKOG1124, Eukaryota
GeneTreeiENSGT00940000154697
HOGENOMiCLU_008405_1_0_1
InParanoidiQ5U2X2
KOiK19870
OMAiQYRKAYV
OrthoDBi1070087at2759
PhylomeDBiQ5U2X2
TreeFamiTF106251

Miscellaneous databases

Protein Ontology

More...
PROi
PR:Q5U2X2

Gene expression databases

BgeeiENSRNOG00000010078, Expressed in testis and 20 other tissues

Family and domain databases

Gene3Di1.25.40.10, 3 hits
InterProiView protein in InterPro
IPR025986, RPAP3-like_C
IPR013026, TPR-contain_dom
IPR011990, TPR-like_helical_dom_sf
IPR001440, TPR_1
IPR019734, TPR_repeat
PfamiView protein in Pfam
PF13877, RPAP3_C, 1 hit
PF00515, TPR_1, 2 hits
PF13181, TPR_8, 1 hit
SMARTiView protein in SMART
SM00028, TPR, 8 hits
SUPFAMiSSF48452, SSF48452, 3 hits
PROSITEiView protein in PROSITE
PS50005, TPR, 5 hits
PS50293, TPR_REGION, 4 hits

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSPAG1_RAT
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q5U2X2
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: December 7, 2004
Last modified: August 12, 2020
This is version 103 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome
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