UniProtKB - Q5U2V8 (EMC3_RAT)
ER membrane protein complex subunit 3
Emc3
Functioni
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors. By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes.
By similarityGO - Molecular functioni
- membrane insertase activity Source: Ensembl
GO - Biological processi
- protein insertion into ER membrane by stop-transfer membrane-anchor sequence Source: UniProtKB
- tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
Enzyme and pathway databases
Reactomei | R-RNO-8980692, RHOA GTPase cycle |
Names & Taxonomyi
Protein namesi | Recommended name: ER membrane protein complex subunit 3Alternative name(s): Transmembrane protein 111 |
Gene namesi | Name:Emc3 Synonyms:Tmem111 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1311566, Emc3 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- EMC complex Source: UniProtKB
- integral component of endoplasmic reticulum membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 14 | LumenalBy similarityAdd BLAST | 13 | |
Transmembranei | 15 – 38 | HelicalBy similarityAdd BLAST | 24 | |
Topological domaini | 39 – 114 | CytoplasmicBy similarityAdd BLAST | 76 | |
Transmembranei | 115 – 130 | HelicalBy similarityAdd BLAST | 16 | |
Topological domaini | 131 – 168 | LumenalBy similarityAdd BLAST | 38 | |
Transmembranei | 169 – 187 | HelicalBy similarityAdd BLAST | 19 | |
Topological domaini | 188 – 261 | CytoplasmicBy similarityAdd BLAST | 74 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000211409 | 2 – 261 | ER membrane protein complex subunit 3Add BLAST | 260 |
Proteomic databases
jPOSTi | Q5U2V8 |
PaxDbi | Q5U2V8 |
PTM databases
iPTMneti | Q5U2V8 |
PhosphoSitePlusi | Q5U2V8 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000009934, Expressed in female gonad and 21 other tissues |
Genevisiblei | Q5U2V8, RN |
Interactioni
Subunit structurei
Component of the ER membrane protein complex (EMC).
By similarityProtein-protein interaction databases
IntActi | Q5U2V8, 1 interactor |
STRINGi | 10116.ENSRNOP00000013488 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3188, Eukaryota |
GeneTreei | ENSGT00390000005780 |
HOGENOMi | CLU_060791_0_0_1 |
InParanoidi | Q5U2V8 |
OMAi | SINMIDH |
OrthoDBi | 1482782at2759 |
PhylomeDBi | Q5U2V8 |
Family and domain databases
InterProi | View protein in InterPro IPR008568, EMC3 IPR002809, EMC3/TMCO1 |
PANTHERi | PTHR13116, PTHR13116, 1 hit |
Pfami | View protein in Pfam PF01956, EMC3_TMCO1, 1 hit |
PIRSFi | PIRSF010045, DUF850_TM_euk, 1 hit |
SMARTi | View protein in SMART SM01415, DUF106, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAGPELLLDS NIRLWVVLPI VIITFFVGMI RHYVSILLQS DKKLTQEQVS
60 70 80 90 100
DSQVLIRSRV LRENGKYIPK QSFLTRKYYF NNPEDGFFKK TKRKVVPPSP
110 120 130 140 150
MTDPTMLTDM MKGNVTNVLP MILIGGWINM TFSGFVTTKV PFPLTLRFKP
160 170 180 190 200
MLQQGIELLT LDASWVSSAS WYFLNVFGLR SIYSLILGQD NAADQSRMMQ
210 220 230 240 250
EQMTGAAMAM PADTNKAFKT EWEALELTDH QWALDDVEEE LMARDLHFEG
260
MFKKELQTSI F
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC085846 mRNA Translation: AAH85846.1 |
RefSeqi | NP_001008356.1, NM_001008355.1 |
Genome annotation databases
Ensembli | ENSRNOT00000013488; ENSRNOP00000013488; ENSRNOG00000009934 |
GeneIDi | 312640 |
KEGGi | rno:312640 |
UCSCi | RGD:1311566, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC085846 mRNA Translation: AAH85846.1 |
RefSeqi | NP_001008356.1, NM_001008355.1 |
3D structure databases
SMRi | Q5U2V8 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q5U2V8, 1 interactor |
STRINGi | 10116.ENSRNOP00000013488 |
PTM databases
iPTMneti | Q5U2V8 |
PhosphoSitePlusi | Q5U2V8 |
Proteomic databases
jPOSTi | Q5U2V8 |
PaxDbi | Q5U2V8 |
Genome annotation databases
Ensembli | ENSRNOT00000013488; ENSRNOP00000013488; ENSRNOG00000009934 |
GeneIDi | 312640 |
KEGGi | rno:312640 |
UCSCi | RGD:1311566, rat |
Organism-specific databases
CTDi | 55831 |
RGDi | 1311566, Emc3 |
Phylogenomic databases
eggNOGi | KOG3188, Eukaryota |
GeneTreei | ENSGT00390000005780 |
HOGENOMi | CLU_060791_0_0_1 |
InParanoidi | Q5U2V8 |
OMAi | SINMIDH |
OrthoDBi | 1482782at2759 |
PhylomeDBi | Q5U2V8 |
Enzyme and pathway databases
Reactomei | R-RNO-8980692, RHOA GTPase cycle |
Miscellaneous databases
PROi | PR:Q5U2V8 |
Gene expression databases
Bgeei | ENSRNOG00000009934, Expressed in female gonad and 21 other tissues |
Genevisiblei | Q5U2V8, RN |
Family and domain databases
InterProi | View protein in InterPro IPR008568, EMC3 IPR002809, EMC3/TMCO1 |
PANTHERi | PTHR13116, PTHR13116, 1 hit |
Pfami | View protein in Pfam PF01956, EMC3_TMCO1, 1 hit |
PIRSFi | PIRSF010045, DUF850_TM_euk, 1 hit |
SMARTi | View protein in SMART SM01415, DUF106, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EMC3_RAT | |
Accessioni | Q5U2V8Primary (citable) accession number: Q5U2V8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 23, 2022 | |
This is version 105 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families