Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Zinc finger protein PLAG1

Gene

Plag1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Cooperates with CBFB-MYH11 (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri34 – 56C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri62 – 86C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri92 – 114C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri121 – 143C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri150 – 172C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri185 – 207C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri213 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein PLAG1
Alternative name(s):
Pleiomorphic adenoma gene 1 protein
Gene namesi
Name:Plag1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi1305286 Plag1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002951091 – 499Zinc finger protein PLAG1Add BLAST499

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki244Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Cross-linki263Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity

Post-translational modificationi

Sumoylated with SUMO1; which inhibits transcriptional activity, but does not affect nuclear localization. Blockers of sumoylation pathway such as SENP3 and inactive UBE2I increases transcriptional capacity. Sumoylation is increased in the presence of PIAS1 (By similarity).By similarity
Acetylated by lysine acetyltransferase EP300; which activates transcriptional capacity. Lysine residues that are sumoylated also seem to be target for acetylation (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiQ5U2T6
PRIDEiQ5U2T6

Expressioni

Tissue specificityi

Expressed in heart, spleen, lung, kidney, brain, testis and epididymis but not in salivary glands.

Gene expression databases

BgeeiENSRNOG00000008846 Expressed in 9 organ(s), highest expression level in heart
GenevisibleiQ5U2T6 RN

Interactioni

Subunit structurei

Interacts with KPNA2, which escorts protein to the nucleus via interaction with nuclear localization signal. Interacts with E3 SUMO-protein ligase PIAS1, PIAS2 and PIAS4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011721

Structurei

3D structure databases

ProteinModelPortaliQ5U2T6
SMRiQ5U2T6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 84Interaction with KPNA2By similarityAdd BLAST83
Regioni41 – 242Decreased nuclear import with localization in the nucleus but also in the cytoplasmBy similarityAdd BLAST202
Regioni243 – 499Activates transcription; Inhibition of nuclear import due to lack of NLS and KPNA2 interactionBy similarityAdd BLAST257
Regioni243 – 383Repression domain; contains 3 sumoylation motifs and massively decrease transcription activityBy similarityAdd BLAST141
Regioni384 – 499Massively activates transcriptionBy similarityAdd BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi22 – 25Nuclear localization signalBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi369 – 488Ser-richAdd BLAST120

Domaini

C2H2-type zinc fingers 3 interacts with DNA-binding site G-clusterinc fingers. C2H2-type zinc fingers 6 and 7 interact with DNA-binding site core sequence (By similarity).By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri34 – 56C2H2-type 1PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri62 – 86C2H2-type 2PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri92 – 114C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri121 – 143C2H2-type 4PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri150 – 172C2H2-type 5PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri185 – 207C2H2-type 6PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri213 – 236C2H2-type 7PROSITE-ProRule annotationAdd BLAST24

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00390000013640
HOGENOMiHOG000294191
HOVERGENiHBG053608
InParanoidiQ5U2T6
KOiK19484
OMAiIDMDAVH
OrthoDBiEOG091G061O
PhylomeDBiQ5U2T6
TreeFamiTF332024

Family and domain databases

InterProiView protein in InterPro
IPR027765 PLAG1
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR44197:SF2 PTHR44197:SF2, 1 hit
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 7 hits
SUPFAMiSSF57667 SSF57667, 4 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 7 hits

Sequencei

Sequence statusi: Complete.

Q5U2T6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATVIPGDLS EVRDTQKAPS GKRKRGESKP RKNFPCQLCD KAFNSVEKLK
60 70 80 90 100
VHSFSHTGER PYKCTHQDCT KAFVSKYKLQ RHMATHSPEK THKCNYCEKM
110 120 130 140 150
FHRKDHLKNH LHTHDPNKET FKCEECGKSY NTKLGFKRHL ALHAATSGDL
160 170 180 190 200
TCKVCLQTFE STGVLLEHLK SHAGKSSGGV KEKKHQCEHC ERRFYTRKDV
210 220 230 240 250
RRHMVVHTGR KDFLCQYCAQ RFGRKDHLTR HMKKSHNQEL LKVKTEPVDF
260 270 280 290 300
LDPFTCNMSV PIKDELLPVM SLPSSELLSK PFTNTLQLNL YNTPFQSMQS
310 320 330 340 350
SGSTHQMITT LPLGMTCPID MDTVHPSHHL AFKCPFSSTS YAISIPEKEQ
360 370 380 390 400
PLKGEIESYL MELQGGAPSS SQDSQASSSK LGLEPQSGSP DDGAGDLSLS
410 420 430 440 450
KSSISISDPL NTPALDFSQL FNFIPLNGPP YNPLSVGSLG MSYSQDEAHS
460 470 480 490
SVSQLPTQTQ DLQDPANTVG LGSLHSLSAA FTSSLSSSTT LPRFHQAFQ
Length:499
Mass (Da):55,640
Last modified:December 7, 2004 - v1
Checksum:i8EC2FA9F25A1CC8B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085871 mRNA Translation: AAH85871.1
RefSeqiNP_001008317.1, NM_001008316.1
XP_008761747.1, XM_008763525.2
XP_008761748.1, XM_008763526.2
XP_017448710.1, XM_017593221.1
XP_017448711.1, XM_017593222.1
XP_017448712.1, XM_017593223.1
UniGeneiRn.234443

Genome annotation databases

EnsembliENSRNOT00000011721; ENSRNOP00000011721; ENSRNOG00000008846
GeneIDi297804
KEGGirno:297804
UCSCiRGD:1305286 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085871 mRNA Translation: AAH85871.1
RefSeqiNP_001008317.1, NM_001008316.1
XP_008761747.1, XM_008763525.2
XP_008761748.1, XM_008763526.2
XP_017448710.1, XM_017593221.1
XP_017448711.1, XM_017593222.1
XP_017448712.1, XM_017593223.1
UniGeneiRn.234443

3D structure databases

ProteinModelPortaliQ5U2T6
SMRiQ5U2T6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000011721

Proteomic databases

PaxDbiQ5U2T6
PRIDEiQ5U2T6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011721; ENSRNOP00000011721; ENSRNOG00000008846
GeneIDi297804
KEGGirno:297804
UCSCiRGD:1305286 rat

Organism-specific databases

CTDi5324
RGDi1305286 Plag1

Phylogenomic databases

eggNOGiKOG1721 Eukaryota
COG5048 LUCA
GeneTreeiENSGT00390000013640
HOGENOMiHOG000294191
HOVERGENiHBG053608
InParanoidiQ5U2T6
KOiK19484
OMAiIDMDAVH
OrthoDBiEOG091G061O
PhylomeDBiQ5U2T6
TreeFamiTF332024

Miscellaneous databases

PROiPR:Q5U2T6

Gene expression databases

BgeeiENSRNOG00000008846 Expressed in 9 organ(s), highest expression level in heart
GenevisibleiQ5U2T6 RN

Family and domain databases

InterProiView protein in InterPro
IPR027765 PLAG1
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
PANTHERiPTHR44197:SF2 PTHR44197:SF2, 1 hit
PfamiView protein in Pfam
PF00096 zf-C2H2, 3 hits
SMARTiView protein in SMART
SM00355 ZnF_C2H2, 7 hits
SUPFAMiSSF57667 SSF57667, 4 hits
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 7 hits
PS50157 ZINC_FINGER_C2H2_2, 7 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPLAG1_RAT
AccessioniPrimary (citable) accession number: Q5U2T6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: December 7, 2004
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again