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Protein

Ribonuclease H2 subunit A

Gene

Rnaseh2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Divalent metal cationBy similarity1
Metal bindingi35Divalent metal cationBy similarity1
Metal bindingi142Divalent metal cationBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMetal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H2 subunit A (EC:3.1.26.4)
Short name:
RNase H2 subunit A
Alternative name(s):
Ribonuclease HI large subunit
Short name:
RNase HI large subunit
Ribonuclease HI subunit A
Gene namesi
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 19

Organism-specific databases

RGDi1307248 Rnaseh2a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001117121 – 301Ribonuclease H2 subunit AAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei217PhosphothreonineBy similarity1
Modified residuei258PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ5U209
PRIDEiQ5U209

PTM databases

PhosphoSitePlusiQ5U209

Expressioni

Gene expression databases

BgeeiENSRNOG00000003504 Expressed in 10 organ(s), highest expression level in testis
GenevisibleiQ5U209 RN

Interactioni

Subunit structurei

The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049148

Structurei

3D structure databases

ProteinModelPortaliQ5U209
SMRiQ5U209
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase HII family. Eukaryotic subfamily.Curated

Phylogenomic databases

eggNOGiKOG2299 Eukaryota
COG0164 LUCA
GeneTreeiENSGT00390000010768
HOGENOMiHOG000100290
HOVERGENiHBG023585
InParanoidiQ5U209
KOiK10743
OMAiREECRFF
PhylomeDBiQ5U209

Family and domain databases

Gene3Di1.10.10.460, 1 hit
3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR004649 RNase_H2_suA
IPR001352 RNase_HII/HIII
IPR024567 RNase_HII/HIII_dom
IPR023160 RNase_HII_hlx-loop-hlx_cap_dom
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10954 PTHR10954, 1 hit
PfamiView protein in Pfam
PF01351 RNase_HII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00729 TIGR00729, 1 hit

Sequencei

Sequence statusi: Complete.

Q5U209-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDLSELERDN TGRCRLSSPV PAVCLKEPCV LGVDEAGRGP VLGPMVYAIC
60 70 80 90 100
YCPLSRLADL EALKVADSKT LTENERERLF AKMEEDGDFV GWALDILSPN
110 120 130 140 150
LISTSMLGRV KYNLNSMSHD TAAGLIQHAM DQNVKVTQVF VDTVGMPETY
160 170 180 190 200
QARLQQRFPG IEVTVKAKAD SLFPVVSAAS IIAKVARDQA VKNWQFVESL
210 220 230 240 250
QGLDSDYGSG YPNDPKTKAW LRKHVDPVFG FPQFVRFSWS TAQAILEKEA
260 270 280 290 300
ESVTWEDSAA EEDPEGPGRI TSYFSQGPQA CRPQVSHKYF QERGLETATS

L
Length:301
Mass (Da):33,286
Last modified:December 7, 2004 - v1
Checksum:i57796835026A5201
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086345 mRNA Translation: AAH86345.1
BC086539 mRNA Translation: AAH86539.1
RefSeqiNP_001013252.1, NM_001013234.1
XP_017456820.1, XM_017601331.1
XP_017456821.1, XM_017601332.1
XP_017456822.1, XM_017601333.1
XP_017456823.1, XM_017601334.1
XP_017456824.1, XM_017601335.1
XP_017456825.1, XM_017601336.1
XP_017456826.1, XM_017601337.1
XP_017456827.1, XM_017601338.1
UniGeneiRn.3693

Genome annotation databases

EnsembliENSRNOT00000046181; ENSRNOP00000049148; ENSRNOG00000003504
ENSRNOT00000083159; ENSRNOP00000070694; ENSRNOG00000003504
GeneIDi364974
KEGGirno:364974

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC086345 mRNA Translation: AAH86345.1
BC086539 mRNA Translation: AAH86539.1
RefSeqiNP_001013252.1, NM_001013234.1
XP_017456820.1, XM_017601331.1
XP_017456821.1, XM_017601332.1
XP_017456822.1, XM_017601333.1
XP_017456823.1, XM_017601334.1
XP_017456824.1, XM_017601335.1
XP_017456825.1, XM_017601336.1
XP_017456826.1, XM_017601337.1
XP_017456827.1, XM_017601338.1
UniGeneiRn.3693

3D structure databases

ProteinModelPortaliQ5U209
SMRiQ5U209
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000049148

PTM databases

PhosphoSitePlusiQ5U209

Proteomic databases

PaxDbiQ5U209
PRIDEiQ5U209

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000046181; ENSRNOP00000049148; ENSRNOG00000003504
ENSRNOT00000083159; ENSRNOP00000070694; ENSRNOG00000003504
GeneIDi364974
KEGGirno:364974

Organism-specific databases

CTDi10535
RGDi1307248 Rnaseh2a

Phylogenomic databases

eggNOGiKOG2299 Eukaryota
COG0164 LUCA
GeneTreeiENSGT00390000010768
HOGENOMiHOG000100290
HOVERGENiHBG023585
InParanoidiQ5U209
KOiK10743
OMAiREECRFF
PhylomeDBiQ5U209

Miscellaneous databases

PROiPR:Q5U209

Gene expression databases

BgeeiENSRNOG00000003504 Expressed in 10 organ(s), highest expression level in testis
GenevisibleiQ5U209 RN

Family and domain databases

Gene3Di1.10.10.460, 1 hit
3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR004649 RNase_H2_suA
IPR001352 RNase_HII/HIII
IPR024567 RNase_HII/HIII_dom
IPR023160 RNase_HII_hlx-loop-hlx_cap_dom
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10954 PTHR10954, 1 hit
PfamiView protein in Pfam
PF01351 RNase_HII, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
TIGRFAMsiTIGR00729 TIGR00729, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNH2A_RAT
AccessioniPrimary (citable) accession number: Q5U209
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: December 7, 2004
Last modified: September 12, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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