UniProtKB - Q5U0F4 (Q5U0F4_HUMAN)
Eukaryotic translation initiation factor 3 subunit I
EIF3S2
Functioni
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.
UniRule annotationGO - Molecular functioni
- translation initiation factor activity Source: UniProtKB-UniRule
GO - Biological processi
- formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
Keywordsi
Molecular function | Initiation factorUniRule annotationARBA annotation |
Biological process | Protein biosynthesis |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic translation initiation factor 3 subunit IUniRule annotationShort name: eIF3iUniRule annotation Alternative name(s): Eukaryotic translation initiation factor 3 subunit 2UniRule annotation eIF-3-betaUniRule annotation eIF3 p36UniRule annotation |
Gene namesi | Name:EIF3S2UniRule annotationImported Synonyms:EIF3IUniRule annotation ORF Names:hCG_41608Imported |
Organismi | Homo sapiens (Human)Imported |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Organism-specific databases
VEuPathDBi | HostDB:ENSG00000084623 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- eukaryotic 43S preinitiation complex Source: UniProtKB-UniRule
- eukaryotic 48S preinitiation complex Source: UniProtKB-UniRule
- eukaryotic translation initiation factor 3 complex Source: UniProtKB-UniRule
Keywords - Cellular componenti
CytoplasmUniRule annotationPTM / Processingi
Post-translational modificationi
Keywords - PTMi
PhosphoproteinUniRule annotationExpressioni
Gene expression databases
Bgeei | ENSG00000084623, Expressed in left adrenal gland and 244 other tissues |
Interactioni
Subunit structurei
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3.
UniRule annotationProtein-protein interaction databases
IntActi | Q5U0F4, 4 interactors |
Structurei
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 6 – 47 | WDPROSITE-ProRule annotationAdd BLAST | 42 | |
Repeati | 48 – 89 | WDPROSITE-ProRule annotationAdd BLAST | 42 | |
Repeati | 184 – 225 | WDPROSITE-ProRule annotationAdd BLAST | 42 | |
Repeati | 281 – 311 | WDPROSITE-ProRule annotationAdd BLAST | 31 |
Sequence similaritiesi
Keywords - Domaini
RepeatARBA annotation, WD repeatPROSITE-ProRule annotationARBA annotationPhylogenomic databases
HOGENOMi | CLU_043845_0_0_1 |
OMAi | ITPKKDF |
OrthoDBi | 866359at2759 |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_03008, eIF3i, 1 hit |
InterProi | View protein in InterPro IPR027525, eIF3i IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 3 hits |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 4 hits |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKPILLQGHE RSITQIKYNR EGDLLFTVAK DPIVNVWYSV NGERLGTYMG
60 70 80 90 100
HTGAVWCVDA DWDTKHVLTG SADNSCRLWD CETGKQLALL KTNSAVRTCG
110 120 130 140 150
FDFGGNIIMF STDKQMGYQC FVSFFDLRDP SQIDNNEPYM KIPCNDSKIT
160 170 180 190 200
SAVWGPLGEC IIAGHESGEL NQYSAKSGEV LVNVKEHSRQ INDIQLSRDM
210 220 230 240 250
TMFVTASKDN TAKLFDSTTL EHQKTFRTER PVNSAALSPN YDHVVLGGGQ
260 270 280 290 300
EAMDVTTTST RIGKFEARFF HLAFEEEFGR VKGHFGPINS VAFHPDGKSY
310 320
SSGGEDGYVR IHYFDPQYFE FEFEA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT019597 mRNA Translation: AAV38404.1 AK289356 mRNA Translation: BAF82045.1 AK289882 mRNA Translation: BAF82571.1 CH471059 Genomic DNA Translation: EAX07553.1 |
RefSeqi | NP_003748.1, NM_003757.3 |
Genome annotation databases
GeneIDi | 8668 |
KEGGi | hsa:8668 |
UCSCi | uc009vuc.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BT019597 mRNA Translation: AAV38404.1 AK289356 mRNA Translation: BAF82045.1 AK289882 mRNA Translation: BAF82571.1 CH471059 Genomic DNA Translation: EAX07553.1 |
RefSeqi | NP_003748.1, NM_003757.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
IntActi | Q5U0F4, 4 interactors |
Protocols and materials databases
Antibodypediai | 31260, 176 antibodies from 27 providers |
DNASUi | 8668 |
Genome annotation databases
GeneIDi | 8668 |
KEGGi | hsa:8668 |
UCSCi | uc009vuc.4, human |
Organism-specific databases
CTDi | 8668 |
PharmGKBi | PA162384875 |
VEuPathDBi | HostDB:ENSG00000084623 |
Phylogenomic databases
HOGENOMi | CLU_043845_0_0_1 |
OMAi | ITPKKDF |
OrthoDBi | 866359at2759 |
Miscellaneous databases
BioGRID-ORCSi | 8668, 800 hits in 1024 CRISPR screens |
ChiTaRSi | EIF3I, human |
GenomeRNAii | 8668 |
Gene expression databases
Bgeei | ENSG00000084623, Expressed in left adrenal gland and 244 other tissues |
Family and domain databases
Gene3Di | 2.130.10.10, 1 hit |
HAMAPi | MF_03008, eIF3i, 1 hit |
InterProi | View protein in InterPro IPR027525, eIF3i IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
Pfami | View protein in Pfam PF00400, WD40, 3 hits |
SMARTi | View protein in SMART SM00320, WD40, 5 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 4 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | Q5U0F4_HUMAN | |
Accessioni | Q5U0F4Primary (citable) accession number: Q5U0F4 | |
Entry historyi | Integrated into UniProtKB/TrEMBL: | February 5, 2008 |
Last sequence update: | February 5, 2008 | |
Last modified: | January 19, 2022 | |
This is version 128 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Unreviewed (UniProtKB/TrEMBL) | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |