UniProtKB - Q5TCZ1 (SPD2A_HUMAN)

BLAST

>sp|Q5TCZ1|SPD2A_HUMAN SH3 and PX domain-containing protein 2A OS=Homo sapiens OX=9606 GN=SH3PXD2A PE=1 SV=1
MLAYCVQDATVVDVEKRRNPSKHYVYIINVTWSDSTSQTIYRRYSKFFDLQMQLLDKFPI
EGGQKDPKQRIIPFLPGKILFRRSHIRDVAVKRLKPIDEYCRALVRLPPHISQCDEVFRF
FEARPEDVNPPKEDYGSSKRKSVWLSSWAESPKKDVTGADATAEPMILEQYVVVSNYKKQ
ENSELSLQAGEVVDVIEKNESGWWFVSTSEEQGWVPATYLEAQNGTRDDSDINTSKTGEV
SKRRKAHLRRLDRRWTLGGMVNRQHSREEKYVTVQPYTSQSKDEIGFEKGVTVEVIRKNL
EGWWYIRYLGKEGWAPASYLKKAKDDLPTRKKNLAGPVEIIGNIMEISNLLNKKASGDKE
TPPAEGEGHEAPIAKKEISLPILCNASNGSAVGVPDRTVSRLAQGSPAVARIAPQRAQIS
SPNLRTRPPPRRESSLGFQLPKPPEPPSVEVEYYTIAEFQSCISDGISFRGGQKAEVIDK
NSGGWWYVQIGEKEGWAPASYIDKRKKPNLSRRTSTLTRPKVPPPAPPSKPKEAEEGPTG
ASESQDSPRKLKYEEPEYDIPAFGFDSEPELSEEPVEDRASGERRPAQPHRPSPASSLQR
ARFKVGESSEDVALEEETIYENEGFRPYAEDTLSARGSSGDSDSPGSSSLSLTRKNSPKS
GSPKSSSLLKLKAEKNAQAEMGKNHSSASFSSSITINTTCCSSSSSSSSSLSKTSGDLKP
RSASDAGIRGTPKVRAKKDADANAGLTSCPRAKPSVRPKPFLNRAESQSQEKMDISTLRR
QLRPTGQLRGGLKGSKSEDSELPPQTASEAPSEGSRRSSSDLITLPATTPPCPTKKEWEG
PATSYMTCSAYQKVQDSEISFPAGVEVQVLEKQESGWWYVRFGELEGWAPSHYLVLDENE
QPDPSGKELDTVPAKGRQNEGKSDSLEKIERRVQALNTVNQSKKATPPIPSKPPGGFGKT
SGTPAVKMRNGVRQVAVRPQSVFVSPPPKDNNLSCALRRNESLTATDGLRGVRRNSSFST
ARSAAAEAKGRLAERAASQGSDSPLLPAQRNSIPVSPVRPKPIEKSQFIHNNLKDVYVSI
ADYEGDEETAGFQEGVSMEVLERNPNGWWYCQILDGVKPFKGWVPSNYLEKKN

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History
Entry version 137 (07 Nov 2018)
Sequence version 1 (21 Dec 2004)
Previous versions | rss
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Protein

SH3 and PX domain-containing protein 2A

Gene

SH3PXD2A

Organism

Homo sapiens (Human)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Adapter protein involved in invadopodia and podosome formation, extracellular matrix degradation and invasiveness of some cancer cells. Binds matrix metalloproteinases (ADAMs), NADPH oxidases (NOXs) and phosphoinositides. Acts as an organizer protein that allows NOX1- or NOX3-dependent reactive oxygen species (ROS) generation and ROS localization. In association with ADAM12, mediates the neurotoxic effect of amyloid-beta peptide.5 Publications

GO - Molecular functionⁱ

phosphatidylinositol-3,4-bisphosphate binding Source: Ensembl
phosphatidylinositol-3-phosphate binding Source: Ensembl
phosphatidylinositol-4,5-bisphosphate binding Source: Ensembl
phosphatidylinositol-4-phosphate binding Source: Ensembl
phosphatidylinositol-5-phosphate binding Source: Ensembl
phosphatidylinositol binding
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protease binding Source: Ensembl
superoxide-generating NADPH oxidase activator activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

extracellular matrix organization Source: Reactome
osteoclast fusion
Source: CACAO
Inferred from mutant phenotypeⁱ
- 22584907
superoxide metabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 19755710

View the complete GO annotation on QuickGO ...

Enzyme and pathway databases

Reactomeⁱ	R-HSA-8941237 Invadopodia formation

Reactomeⁱ

R-HSA-8941237 Invadopodia formation

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: SH3 and PX domain-containing protein 2A Alternative name(s): Adapter protein TKS5 Five SH3 domain-containing protein SH3 multiple domains protein 1 Tyrosine kinase substrate with five SH3 domains
Gene namesⁱ	Name:SH3PXD2A Synonyms:FISH, KIAA0418, SH3MD1, TKS5
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 10

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000107957.16
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:23664 SH3PXD2A
neXtProtⁱ	NX_Q5TCZ1

Keywords - Cellular componentⁱ

Cell junction, Cell projection, Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	42	R → A: Loss of binding to (PtdIns(3)P) and (PtdIns(3,4)P2). 1 Publication		1
Mutagenesisⁱ	93	R → A: Loss of binding to (PtdIns(3)P) and (PtdIns(3,4)P2). 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	9644
MalaCards human disease database More...MalaCardsⁱ	SH3PXD2A
Open Targets More...OpenTargetsⁱ	ENSG00000107957
Orphanetⁱ	3148 Malignant peripheral nerve sheath tumor
PharmGKBⁱ	PA134956816

Polymorphism and mutation databases

BioMutaⁱ	SH3PXD2A
DMDMⁱ	74746151

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000278488	1 – 1133	SH3 and PX domain-containing protein 2AAdd BLAST		1133

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	256	PhosphothreonineBy similarity	1
Modified residueⁱ	406	PhosphoserineCombined sources	1
Modified residueⁱ	421	PhosphoserineCombined sources	1
Modified residueⁱ	547	PhosphoserineCombined sources	1
Modified residueⁱ	567	PhosphoserineCombined sources	1
Modified residueⁱ	593	PhosphoserineCombined sources	1
Modified residueⁱ	644	PhosphoserineCombined sources	1
Modified residueⁱ	731	PhosphothreonineCombined sources	1
Modified residueⁱ	767	PhosphoserineBy similarity	1
Modified residueⁱ	769	PhosphoserineBy similarity	1
Modified residueⁱ	819	PhosphoserineBy similarity	1
Modified residueⁱ	829	PhosphothreonineBy similarity	1
Modified residueⁱ	1002	PhosphoserineCombined sources	1
Modified residueⁱ	1016	PhosphoserineCombined sources	1
Modified residueⁱ	1017	PhosphoserineCombined sources	1
Modified residueⁱ	1038	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Tyrosine phosphorylated by SRC. Phosphorylation plays a regulatory role in the protein localization. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region. Phosphorylated on serine upon DNA damage, probably by ATM or ATR.

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q5TCZ1
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q5TCZ1
PaxDbⁱ	Q5TCZ1
PeptideAtlas More...PeptideAtlasⁱ	Q5TCZ1
PRIDEⁱ	Q5TCZ1
ProteomicsDBⁱ	64997 64998 [Q5TCZ1-2] 64999 [Q5TCZ1-3]

PTM databases

iPTMnetⁱ	Q5TCZ1
PhosphoSitePlusⁱ	Q5TCZ1

Expressionⁱ

Tissue specificityⁱ

Found in several cancer cell lines, particularly invasive breast carcinomas and melanomas.1 Publication

Gene expression databases

Bgeeⁱ	ENSG00000107957 Expressed in 219 organ(s), highest expression level in uterine cervix
CleanExⁱ	HS_SH3PXD2A
ExpressionAtlasⁱ	Q5TCZ1 baseline and differential
Genevisibleⁱ	Q5TCZ1 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA037922 HPA037923

HPAⁱ

HPA037922
HPA037923

Interactionⁱ

Subunit structureⁱ

Interacts (via N-terminus) with CYBA (By similarity). Interacts with ADAM12, ADAM15 and ADAM19. Interacts with NOXO1. Interacts (via SH3 domains) with NOXA1. Interacts with FASLG.By similarity5 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
ADAM15	Q13444	2	EBI-2483234,EBI-77818
Dnm1	P21575	2	EBI-7014859,EBI-80070	From Rattus norvegicus.
SOS1	Q07889	5	EBI-7014859,EBI-297487

GO - Molecular functionⁱ

protease binding Source: Ensembl

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	115002, 90 interactors
IntActⁱ	Q5TCZ1, 14 interactors
Molecular INTeraction database More...MINTⁱ	Q5TCZ1
STRINGⁱ	9606.ENSP00000348215

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	181 – 184	Combined sources	4
Beta strandⁱ	194 – 198	Combined sources	5
Beta strandⁱ	202 – 207	Combined sources	6
Beta strandⁱ	212 – 216	Combined sources	5
Helixⁱ	217 – 219	Combined sources	3
Beta strandⁱ	221 – 223	Combined sources	3
Beta strandⁱ	268 – 272	Combined sources	5
Beta strandⁱ	292 – 295	Combined sources	4
Beta strandⁱ	300 – 308	Combined sources	9
Beta strandⁱ	311 – 316	Combined sources	6
Helixⁱ	317 – 319	Combined sources	3
Beta strandⁱ	843 – 847	Combined sources	5
Beta strandⁱ	866 – 872	Combined sources	7
Beta strandⁱ	876 – 882	Combined sources	7
Beta strandⁱ	885 – 890	Combined sources	6
Turnⁱ	891 – 893	Combined sources	3
Beta strandⁱ	1075 – 1079	Combined sources	5
Beta strandⁱ	1087 – 1089	Combined sources	3
Beta strandⁱ	1097 – 1100	Combined sources	4
Beta strandⁱ	1107 – 1113	Combined sources	7
Beta strandⁱ	1116 – 1118	Combined sources	3
Beta strandⁱ	1120 – 1125	Combined sources	6
Helixⁱ	1126 – 1128	Combined sources	3
Beta strandⁱ	1129 – 1131	Combined sources	3

Show more details

3D structure databases

ProteinModelPortalⁱ	Q5TCZ1
SMRⁱ	Q5TCZ1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ	Q5TCZ1

EvolutionaryTraceⁱ

Q5TCZ1

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	4 – 128	PXPROSITE-ProRule annotationAdd BLAST	125
Domainⁱ	166 – 225	SH3 1PROSITE-ProRule annotationAdd BLAST	60
Domainⁱ	266 – 325	SH3 2PROSITE-ProRule annotationAdd BLAST	60
Domainⁱ	448 – 507	SH3 3PROSITE-ProRule annotationAdd BLAST	60
Domainⁱ	840 – 899	SH3 4PROSITE-ProRule annotationAdd BLAST	60
Domainⁱ	1072 – 1133	SH3 5PROSITE-ProRule annotationAdd BLAST	62

Coiled coil

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Coiled coilⁱ	917 – 946	Sequence analysisAdd BLAST		30

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	634 – 724	Ser-richAdd BLAST		91

Domainⁱ

The PX domain is required for podosome localization because of its ability to bind phosphatidylinositol 3-phosphate (PtdIns₃P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) and, to a lesser extent, phosphatidylinositol 4-phosphate (PtdIns₄P), phosphatidylinositol 5-phosphate (PtdIns₅P), and phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Binds to the third intramolecular SH3 domain (By similarity).By similarity

The fifth SH3 domain mediates binding with ADAM12, ADAM15 and ADAM19.2 Publications

Sequence similaritiesⁱ

Belongs to the SH3PXD2 family.Curated

Keywords - Domainⁱ

Coiled coil, Repeat, SH3 domain

Phylogenomic databases

eggNOGⁱ	KOG4773 Eukaryota ENOG410YBFF LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063010
HOGENOMⁱ	HOG000154376
HOVERGENⁱ	HBG089589
InParanoidⁱ	Q5TCZ1
OMAⁱ	PTQRNGI
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G00YX
PhylomeDBⁱ	Q5TCZ1
TreeFamⁱ	TF329347

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06888 PX_FISH, 1 hit cd12074 SH3_Tks5_1, 1 hit cd12077 SH3_Tks5_2, 1 hit cd12079 SH3_Tks5_3, 1 hit cd12019 SH3_Tks5_4, 1 hit cd12020 SH3_Tks5_5, 1 hit
Gene3Dⁱ	3.30.1520.10, 1 hit
InterProⁱ	View protein in InterPro IPR001683 Phox IPR036871 PX_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR037961 SH3PXD2_PX IPR034917 SH3PXD2A IPR035450 SH3PXD2A_SH3_1 IPR035452 SH3PXD2A_SH3_2 IPR035449 SH3PXD2A_SH3_3 IPR035453 SH3PXD2A_SH3_4 IPR035454 SH3PXD2A_SH3_5
PANTHERⁱ	PTHR15706:SF7 PTHR15706:SF7, 3 hits
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00787 PX, 1 hit PF00018 SH3_1, 4 hits
SMARTⁱ	View protein in SMART SM00312 PX, 1 hit SM00326 SH3, 5 hits
SUPFAMⁱ	SSF50044 SSF50044, 5 hits SSF64268 SSF64268, 1 hit
PROSITEⁱ	View protein in PROSITE PS50195 PX, 1 hit PS50002 SH3, 5 hits

Sequences (3+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 3 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 1 (identifier: Q5TCZ1-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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        10         20         30         40         50
MLAYCVQDAT VVDVEKRRNP SKHYVYIINV TWSDSTSQTI YRRYSKFFDL 
        60         70         80         90        100
QMQLLDKFPI EGGQKDPKQR IIPFLPGKIL FRRSHIRDVA VKRLKPIDEY 
       110        120        130        140        150
CRALVRLPPH ISQCDEVFRF FEARPEDVNP PKEDYGSSKR KSVWLSSWAE 
       160        170        180        190        200
SPKKDVTGAD ATAEPMILEQ YVVVSNYKKQ ENSELSLQAG EVVDVIEKNE 
       210        220        230        240        250
SGWWFVSTSE EQGWVPATYL EAQNGTRDDS DINTSKTGEV SKRRKAHLRR 
       260        270        280        290        300
LDRRWTLGGM VNRQHSREEK YVTVQPYTSQ SKDEIGFEKG VTVEVIRKNL 
       310        320        330        340        350
EGWWYIRYLG KEGWAPASYL KKAKDDLPTR KKNLAGPVEI IGNIMEISNL 
       360        370        380        390        400
LNKKASGDKE TPPAEGEGHE APIAKKEISL PILCNASNGS AVGVPDRTVS 
       410        420        430        440        450
RLAQGSPAVA RIAPQRAQIS SPNLRTRPPP RRESSLGFQL PKPPEPPSVE 
       460        470        480        490        500
VEYYTIAEFQ SCISDGISFR GGQKAEVIDK NSGGWWYVQI GEKEGWAPAS 
       510        520        530        540        550
YIDKRKKPNL SRRTSTLTRP KVPPPAPPSK PKEAEEGPTG ASESQDSPRK 
       560        570        580        590        600
LKYEEPEYDI PAFGFDSEPE LSEEPVEDRA SGERRPAQPH RPSPASSLQR 
       610        620        630        640        650
ARFKVGESSE DVALEEETIY ENEGFRPYAE DTLSARGSSG DSDSPGSSSL 
       660        670        680        690        700
SLTRKNSPKS GSPKSSSLLK LKAEKNAQAE MGKNHSSASF SSSITINTTC 
       710        720        730        740        750
CSSSSSSSSS LSKTSGDLKP RSASDAGIRG TPKVRAKKDA DANAGLTSCP 
       760        770        780        790        800
RAKPSVRPKP FLNRAESQSQ EKMDISTLRR QLRPTGQLRG GLKGSKSEDS 
       810        820        830        840        850
ELPPQTASEA PSEGSRRSSS DLITLPATTP PCPTKKEWEG PATSYMTCSA 
       860        870        880        890        900
YQKVQDSEIS FPAGVEVQVL EKQESGWWYV RFGELEGWAP SHYLVLDENE 
       910        920        930        940        950
QPDPSGKELD TVPAKGRQNE GKSDSLEKIE RRVQALNTVN QSKKATPPIP 
       960        970        980        990       1000
SKPPGGFGKT SGTPAVKMRN GVRQVAVRPQ SVFVSPPPKD NNLSCALRRN 
      1010       1020       1030       1040       1050
ESLTATDGLR GVRRNSSFST ARSAAAEAKG RLAERAASQG SDSPLLPAQR 
      1060       1070       1080       1090       1100
NSIPVSPVRP KPIEKSQFIH NNLKDVYVSI ADYEGDEETA GFQEGVSMEV 
      1110       1120       1130 
LERNPNGWWY CQILDGVKPF KGWVPSNYLE KKN

Length:1,133

Mass (Da):125,289

Last modified:December 21, 2004 - v1

Checksum:ⁱD485F49E9192359C

Isoform 2 (identifier: Q5TCZ1-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
1-165: Missing.
240-267: Missing.

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        10         20         30         40         50
MILEQYVVVS NYKKQENSEL SLQAGEVVDV IEKNESGWWF VSTSEEQGWV 
        60         70         80         90        100
PATYLEAQNG TRDDSDINTS KTGEEEKYVT VQPYTSQSKD EIGFEKGVTV 
       110        120        130        140        150
EVIRKNLEGW WYIRYLGKEG WAPASYLKKA KDDLPTRKKN LAGPVEIIGN 
       160        170        180        190        200
IMEISNLLNK KASGDKETPP AEGEGHEAPI AKKEISLPIL CNASNGSAVG 
       210        220        230        240        250
VPDRTVSRLA QGSPAVARIA PQRAQISSPN LRTRPPPRRE SSLGFQLPKP 
       260        270        280        290        300
PEPPSVEVEY YTIAEFQSCI SDGISFRGGQ KAEVIDKNSG GWWYVQIGEK 
       310        320        330        340        350
EGWAPASYID KRKKPNLSRR TSTLTRPKVP PPAPPSKPKE AEEGPTGASE 
       360        370        380        390        400
SQDSPRKLKY EEPEYDIPAF GFDSEPELSE EPVEDRASGE RRPAQPHRPS 
       410        420        430        440        450
PASSLQRARF KVGESSEDVA LEEETIYENE GFRPYAEDTL SARGSSGDSD 
       460        470        480        490        500
SPGSSSLSLT RKNSPKSGSP KSSSLLKLKA EKNAQAEMGK NHSSASFSSS 
       510        520        530        540        550
ITINTTCCSS SSSSSSSLSK TSGDLKPRSA SDAGIRGTPK VRAKKDADAN 
       560        570        580        590        600
AGLTSCPRAK PSVRPKPFLN RAESQSQEKM DISTLRRQLR PTGQLRGGLK 
       610        620        630        640        650
GSKSEDSELP PQTASEAPSE GSRRSSSDLI TLPATTPPCP TKKEWEGPAT 
       660        670        680        690        700
SYMTCSAYQK VQDSEISFPA GVEVQVLEKQ ESGWWYVRFG ELEGWAPSHY 
       710        720        730        740        750
LVLDENEQPD PSGKELDTVP AKGRQNEGKS DSLEKIERRV QALNTVNQSK 
       760        770        780        790        800
KATPPIPSKP PGGFGKTSGT PAVKMRNGVR QVAVRPQSVF VSPPPKDNNL 
       810        820        830        840        850
SCALRRNESL TATDGLRGVR RNSSFSTARS AAAEAKGRLA ERAASQGSDS 
       860        870        880        890        900
PLLPAQRNSI PVSPVRPKPI EKSQFIHNNL KDVYVSIADY EGDEETAGFQ 
       910        920        930        940
EGVSMEVLER NPNGWWYCQI LDGVKPFKGW VPSNYLEKKN

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Length:940

Mass (Da):102,618

Checksum:ⁱ1CA24D7BC03B8AC3

Isoform 3 (identifier: Q5TCZ1-3) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
240-267: Missing.

« Hide

        10         20         30         40         50
MLAYCVQDAT VVDVEKRRNP SKHYVYIINV TWSDSTSQTI YRRYSKFFDL 
        60         70         80         90        100
QMQLLDKFPI EGGQKDPKQR IIPFLPGKIL FRRSHIRDVA VKRLKPIDEY 
       110        120        130        140        150
CRALVRLPPH ISQCDEVFRF FEARPEDVNP PKEDYGSSKR KSVWLSSWAE 
       160        170        180        190        200
SPKKDVTGAD ATAEPMILEQ YVVVSNYKKQ ENSELSLQAG EVVDVIEKNE 
       210        220        230        240        250
SGWWFVSTSE EQGWVPATYL EAQNGTRDDS DINTSKTGEE EKYVTVQPYT 
       260        270        280        290        300
SQSKDEIGFE KGVTVEVIRK NLEGWWYIRY LGKEGWAPAS YLKKAKDDLP 
       310        320        330        340        350
TRKKNLAGPV EIIGNIMEIS NLLNKKASGD KETPPAEGEG HEAPIAKKEI 
       360        370        380        390        400
SLPILCNASN GSAVGVPDRT VSRLAQGSPA VARIAPQRAQ ISSPNLRTRP 
       410        420        430        440        450
PPRRESSLGF QLPKPPEPPS VEVEYYTIAE FQSCISDGIS FRGGQKAEVI 
       460        470        480        490        500
DKNSGGWWYV QIGEKEGWAP ASYIDKRKKP NLSRRTSTLT RPKVPPPAPP 
       510        520        530        540        550
SKPKEAEEGP TGASESQDSP RKLKYEEPEY DIPAFGFDSE PELSEEPVED 
       560        570        580        590        600
RASGERRPAQ PHRPSPASSL QRARFKVGES SEDVALEEET IYENEGFRPY 
       610        620        630        640        650
AEDTLSARGS SGDSDSPGSS SLSLTRKNSP KSGSPKSSSL LKLKAEKNAQ 
       660        670        680        690        700
AEMGKNHSSA SFSSSITINT TCCSSSSSSS SSLSKTSGDL KPRSASDAGI 
       710        720        730        740        750
RGTPKVRAKK DADANAGLTS CPRAKPSVRP KPFLNRAESQ SQEKMDISTL 
       760        770        780        790        800
RRQLRPTGQL RGGLKGSKSE DSELPPQTAS EAPSEGSRRS SSDLITLPAT 
       810        820        830        840        850
TPPCPTKKEW EGPATSYMTC SAYQKVQDSE ISFPAGVEVQ VLEKQESGWW 
       860        870        880        890        900
YVRFGELEGW APSHYLVLDE NEQPDPSGKE LDTVPAKGRQ NEGKSDSLEK 
       910        920        930        940        950
IERRVQALNT VNQSKKATPP IPSKPPGGFG KTSGTPAVKM RNGVRQVAVR 
       960        970        980        990       1000
PQSVFVSPPP KDNNLSCALR RNESLTATDG LRGVRRNSSF STARSAAAEA 
      1010       1020       1030       1040       1050
KGRLAERAAS QGSDSPLLPA QRNSIPVSPV RPKPIEKSQF IHNNLKDVYV 
      1060       1070       1080       1090       1100
SIADYEGDEE TAGFQEGVSM EVLERNPNGW WYCQILDGVK PFKGWVPSNY 

LEKKN

Note: Gene prediction based on similarity to mouse ortholog and partial transcript data.

Show »

Length:1,105

Mass (Da):121,836

Checksum:ⁱ384ECB9785EE3FAB

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	H0Y507	H0Y507_HUMAN		SH3 and PX domain-containing protei... SH3 and PX domain-containing protein 2A	SH3PXD2A	1,060	Annotation score:

Sequence cautionⁱ

The sequence BAA24848 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature key	Position(s)	DescriptionActions	Length
Natural variantⁱVAR_030781	659	K → Q. Corresponds to variant dbSNP:rs11818820Ensembl.	1
Natural variantⁱVAR_030782	1035	R → Q. Corresponds to variant dbSNP:rs3781365Ensembl.	1
Natural variantⁱVAR_056993	1073	L → P. Corresponds to variant dbSNP:rs12764700Ensembl.	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_023312	1 – 165	Missing in isoform 2. 1 PublicationAdd BLAST		165
Alternative sequenceⁱVSP_023313	240 – 267	Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST		28

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB007878 mRNA Translation: BAA24848.2 Different initiation. AL121929 Genomic DNA No translation available. AL133355 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49623.1 CH471066 Genomic DNA Translation: EAW49624.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS31278.1 [Q5TCZ1-3]
PIRⁱ	T00056
NCBI Reference Sequences More...RefSeqⁱ	NP_055446.2, NM_014631.2 [Q5TCZ1-3] XP_005270351.1, XM_005270294.4
UniGeneⁱ	Hs.678727

Genome annotation databases

Ensemblⁱ	ENST00000355946; ENSP00000348215; ENSG00000107957 [Q5TCZ1-3] ENST00000369774; ENSP00000358789; ENSG00000107957 [Q5TCZ1-1]
GeneIDⁱ	9644
KEGGⁱ	hsa:9644
UCSC genome browser More...UCSCⁱ	uc001kxj.2 human [Q5TCZ1-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q5TCZ1	SH3 and PX domains 2A		CALJA		1141	UniRef90_Q5TCZ1
Uncharacterized protein		Cebus capucinus imitator		1134
Uncharacterized protein		AOTNA		1134
SH3 and PX domain-containing protein 2A isoform X1		Florida manatee		1134
SH3 and PX domains 2A		PANTR		1133
+218
Q5TCZ1-2	Isoform 3 of SH3 and PX domain-containing protein 2A		HUMAN		1105	UniRef90_Q5TCZ1-3
Uncharacterized protein		Cebus capucinus imitator		1106
Uncharacterized protein		AOTNA		1106
SH3 and PX domain-containing protein 2A		LIPVE		1106
SH3 and PX domain-containing protein 2A isoform X3		Florida manatee		1106
+85
Q5TCZ1-3	Isoform 2 of SH3 and PX domain-containing protein 2A		HUMAN		940	UniRef90_Q5TCZ1-3
Uncharacterized protein		Cebus capucinus imitator		1106
Uncharacterized protein		AOTNA		1106
SH3 and PX domain-containing protein 2A		LIPVE		1106
SH3 and PX domain-containing protein 2A isoform X3		Florida manatee		1106
+85

Protein	Similar proteins		Species	Score	Length	Source
Q5TCZ1	SH3 and PX domain-containing protein 2A		MOUSE		1124	UniRef50_Q5TCZ1
SH3 and PX domains 2A		CALJA		1141
SH3 and PX domain-containing protein 2A isoform X1		Florida manatee		1134
Uncharacterized protein		Cebus capucinus imitator		1134
Uncharacterized protein		AOTNA		1134
+419

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB007878 mRNA Translation: BAA24848.2 Different initiation. AL121929 Genomic DNA No translation available. AL133355 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49623.1 CH471066 Genomic DNA Translation: EAW49624.1
CCDSⁱ	CCDS31278.1 [Q5TCZ1-3]
PIRⁱ	T00056
RefSeqⁱ	NP_055446.2, NM_014631.2 [Q5TCZ1-3] XP_005270351.1, XM_005270294.4
UniGeneⁱ	Hs.678727

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2DNU	NMR	-	A	268-325	[»]
	2EGA	NMR	-	A	168-224	[»]
	2EGC	NMR	-	A	1072-1133	[»]
	2EKH	NMR	-	A	842-908	[»]
ProteinModelPortalⁱ	Q5TCZ1
SMRⁱ	Q5TCZ1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	115002, 90 interactors
IntActⁱ	Q5TCZ1, 14 interactors
MINTⁱ	Q5TCZ1
STRINGⁱ	9606.ENSP00000348215

PTM databases

iPTMnetⁱ	Q5TCZ1
PhosphoSitePlusⁱ	Q5TCZ1

Polymorphism and mutation databases

BioMutaⁱ	SH3PXD2A
DMDMⁱ	74746151

Proteomic databases

EPDⁱ	Q5TCZ1
MaxQBⁱ	Q5TCZ1
PaxDbⁱ	Q5TCZ1
PeptideAtlasⁱ	Q5TCZ1
PRIDEⁱ	Q5TCZ1
ProteomicsDBⁱ	64997 64998 [Q5TCZ1-2] 64999 [Q5TCZ1-3]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	9644
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000355946; ENSP00000348215; ENSG00000107957 [Q5TCZ1-3] ENST00000369774; ENSP00000358789; ENSG00000107957 [Q5TCZ1-1]
GeneIDⁱ	9644
KEGGⁱ	hsa:9644
UCSCⁱ	uc001kxj.2 human [Q5TCZ1-1]

Organism-specific databases

CTDⁱ	9644
DisGeNETⁱ	9644
EuPathDBⁱ	HostDB:ENSG00000107957.16
GeneCardsⁱ	SH3PXD2A
HGNCⁱ	HGNC:23664 SH3PXD2A
HPAⁱ	HPA037922 HPA037923
MalaCardsⁱ	SH3PXD2A
neXtProtⁱ	NX_Q5TCZ1
OpenTargetsⁱ	ENSG00000107957
Orphanetⁱ	3148 Malignant peripheral nerve sheath tumor
PharmGKBⁱ	PA134956816
HUGEⁱ	Search...
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG4773 Eukaryota ENOG410YBFF LUCA
GeneTreeⁱ	ENSGT00530000063010
HOGENOMⁱ	HOG000154376
HOVERGENⁱ	HBG089589
InParanoidⁱ	Q5TCZ1
OMAⁱ	PTQRNGI
OrthoDBⁱ	EOG091G00YX
PhylomeDBⁱ	Q5TCZ1
TreeFamⁱ	TF329347

Enzyme and pathway databases

Reactomeⁱ	R-HSA-8941237 Invadopodia formation

Reactomeⁱ

R-HSA-8941237 Invadopodia formation

Miscellaneous databases

ChiTaRSⁱ	SH3PXD2A human
EvolutionaryTraceⁱ	Q5TCZ1
GeneWikiⁱ	SH3PXD2A
GenomeRNAiⁱ	9644
Protein Ontology More...PROⁱ	PR:Q5TCZ1

Gene expression databases

Bgeeⁱ	ENSG00000107957 Expressed in 219 organ(s), highest expression level in uterine cervix
CleanExⁱ	HS_SH3PXD2A
ExpressionAtlasⁱ	Q5TCZ1 baseline and differential
Genevisibleⁱ	Q5TCZ1 HS

Family and domain databases

CDDⁱ	cd06888 PX_FISH, 1 hit cd12074 SH3_Tks5_1, 1 hit cd12077 SH3_Tks5_2, 1 hit cd12079 SH3_Tks5_3, 1 hit cd12019 SH3_Tks5_4, 1 hit cd12020 SH3_Tks5_5, 1 hit
Gene3Dⁱ	3.30.1520.10, 1 hit
InterProⁱ	View protein in InterPro IPR001683 Phox IPR036871 PX_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR037961 SH3PXD2_PX IPR034917 SH3PXD2A IPR035450 SH3PXD2A_SH3_1 IPR035452 SH3PXD2A_SH3_2 IPR035449 SH3PXD2A_SH3_3 IPR035453 SH3PXD2A_SH3_4 IPR035454 SH3PXD2A_SH3_5
PANTHERⁱ	PTHR15706:SF7 PTHR15706:SF7, 3 hits
Pfamⁱ	View protein in Pfam PF00787 PX, 1 hit PF00018 SH3_1, 4 hits
SMARTⁱ	View protein in SMART SM00312 PX, 1 hit SM00326 SH3, 5 hits
SUPFAMⁱ	SSF50044 SSF50044, 5 hits SSF64268 SSF64268, 1 hit
PROSITEⁱ	View protein in PROSITE PS50195 PX, 1 hit PS50002 SH3, 5 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SPD2A_HUMAN
Accessionⁱ	Q5TCZ1Primary (citable) accession number: Q5TCZ1 Secondary accession number(s): D3DR98 , O43302, Q5TCZ2, Q5TDQ8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 20, 2007
	Last sequence update:	December 21, 2004
	Last modified:	November 7, 2018
	This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM

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UniProtKB - Q5TCZ1 (SPD2A_HUMAN)

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SH3 and PX domain-containing protein 2A

SH3PXD2A

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

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GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

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Cytoskeleton

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<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

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Organism-specific databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Coiled coil

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

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PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

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Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ