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Protein

Cytochrome P450 4A22

Gene

CYP4A22

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate and palmitate. Shows no activity towards arachidonic acid and prostaglandin A1. Lacks functional activity in the kidney and does not contribute to renal 20-hydroxyeicosatetraenoic acid (20-HETE) biosynthesis.2 Publications

Caution

Was originally termed CYP4A11.1 Publication

Catalytic activityi

A long-chain fatty acid + [reduced NADPH--hemoprotein reductase] + O2 = an omega-hydroxy-long-chain fatty acid + [oxidized NADPH--hemoprotein reductase] + H2O.1 Publication

Kineticsi

Vmax:15.2 µmol/min/µmol enzyme.
  1. KM=56.7 µM for laurate1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei321Heme (covalent; via 1 link)By similarity1
    Metal bindingi457Iron (heme axial ligand)By similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionMonooxygenase, Oxidoreductase
    LigandHeme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiR-HSA-211935 Fatty acids
    R-HSA-211958 Miscellaneous substrates
    R-HSA-211979 Eicosanoids
    R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 4A22
    Alternative name(s):
    CYPIVA22
    Fatty acid omega-hydroxylase
    Lauric acid omega-hydroxylase
    Long-chain fatty acid omega-monooxygenase (EC:1.14.14.801 Publication)
    Gene namesi
    Name:CYP4A22
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000162365.11
    HGNCiHGNC:20575 CYP4A22
    MIMi615341 gene
    neXtProtiNX_Q5TCH4

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    OpenTargetsiENSG00000162365
    PharmGKBiPA134979692

    Polymorphism and mutation databases

    BioMutaiCYP4A22
    DMDMi74746067

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    PropeptideiPRO_00003434081 – 4By similarity4
    ChainiPRO_00003434095 – 519Cytochrome P450 4A22Add BLAST515

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei440PhosphoserineBy similarity1

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ5TCH4
    PeptideAtlasiQ5TCH4
    PRIDEiQ5TCH4
    ProteomicsDBi64954
    64955 [Q5TCH4-2]

    PTM databases

    iPTMnetiQ5TCH4
    PhosphoSitePlusiQ5TCH4

    Expressioni

    Gene expression databases

    BgeeiENSG00000162365 Expressed in 41 organ(s), highest expression level in right lobe of liver
    ExpressionAtlasiQ5TCH4 baseline and differential
    GenevisibleiQ5TCH4 HS

    Interactioni

    Protein-protein interaction databases

    BioGridi129896, 1 interactor
    STRINGi9606.ENSP00000360958

    Structurei

    3D structure databases

    ProteinModelPortaliQ5TCH4
    SMRiQ5TCH4
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi24 – 31Poly-Leu8
    Compositional biasi131 – 134Poly-Leu4

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiKOG0157 Eukaryota
    COG2124 LUCA
    GeneTreeiENSGT00900000140829
    HOGENOMiHOG000233833
    HOVERGENiHBG000182
    InParanoidiQ5TCH4
    KOiK07425
    PhylomeDBiQ5TCH4
    TreeFamiTF105088

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit

    Sequences (2+)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

    Isoform 1 (identifier: Q5TCH4-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide
            10         20         30         40         50
    MSVSVLSPSR RLGGVSGILQ VTSLLILLLL LIKAAQLYLH RQWLLKALQQ
    60 70 80 90 100
    FPCPPSHWLF GHIQEFQHDQ ELQRIQERVK TFPSACPYWI WGGKVRVQLY
    110 120 130 140 150
    DPDYMKVILG RSDPKSHGSY KFLAPRIGYG LLLLNGQTWF QHRRMLTPAF
    160 170 180 190 200
    HNDILKPYVG LMADSVRVML DKWEELLGQD SPLEVFQHVS LMTLDTIMKS
    210 220 230 240 250
    AFSHQGSIQV DRNSQSYIQA ISDLNSLVFC CMRNAFHEND TIYSLTSAGR
    260 270 280 290 300
    WTHRACQLAH QHTDQVIQLR KAQLQKEGEL EKIKRKRHLD FLDILLLAKM
    310 320 330 340 350
    ENGSILSDKD LRAEVDTFMF EGHDTTASGI SWILYALATH PKHQERCREE
    360 370 380 390 400
    IHGLLGDGAS ITWNHLDQMP YTTMCIKEAL RLYPPVPGIG RELSTPVTFP
    410 420 430 440 450
    DGRSLPKGIM VLLSIYGLHH NPKVWPNLEV FDPSRFAPGS AQHSHAFLPF
    460 470 480 490 500
    SGGSRNCIGK QFAMNQLKVA RALTLLRFEL LPDPTRIPIP MARLVLKSKN
    510
    GIHLRLRRLP NPCEDKDQL
    Length:519
    Mass (Da):59,246
    Last modified:December 21, 2004 - v1
    Checksum:iAA96444A38EE79BC
    GO
    Isoform 2 (identifier: Q5TCH4-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         356-519: Missing.

    Show »
    Length:355
    Mass (Da):40,922
    Checksum:i392C812A7C120920
    GO

    Computationally mapped potential isoform sequencesi

    There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    Q5TCH5Q5TCH5_HUMAN
    Cytochrome P450 4A22
    CYP4A22 RP1-18D14.1-003
    421Annotation score:
    A0A087WUY4A0A087WUY4_HUMAN
    Cytochrome P450 4A22
    CYP4A22
    520Annotation score:
    A0A087WZX9A0A087WZX9_HUMAN
    Cytochrome P450 4A22
    CYP4A22
    357Annotation score:
    A0A0C4DFN9A0A0C4DFN9_HUMAN
    Cytochrome P450 4A22
    CYP4A22
    455Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti2S → A in AAQ21368 (PubMed:15611369).Curated1
    Sequence conflicti114P → S in AAQ21368 (PubMed:15611369).Curated1
    Sequence conflicti225N → S in AAQ21368 (PubMed:15611369).Curated1
    Sequence conflicti232M → V in AAQ21367 (PubMed:15611369).Curated1
    Sequence conflicti238E → Q in AAQ21367 (PubMed:15611369).Curated1
    Sequence conflicti241T → A in AAQ21368 (PubMed:15611369).Curated1
    Sequence conflicti353G → S in AAQ21367 (PubMed:15611369).Curated1
    Sequence conflicti434S → F in AAQ21367 (PubMed:15611369).Curated1
    Sequence conflicti487I → T in AAQ21367 (PubMed:15611369).Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_04434911R → C in allele CYP4A22*2 and CYP4A22*3. 1 PublicationCorresponds to variant dbSNP:rs76011927Ensembl.1
    Natural variantiVAR_061045104Y → F. Corresponds to variant dbSNP:rs61507155Ensembl.1
    Natural variantiVAR_044350121K → R1 PublicationCorresponds to variant dbSNP:rs2758717Ensembl.1
    Natural variantiVAR_044351126R → W in allele CYP4A22*8, allele CYP4A22*9, allele CYP4A22*11, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 PublicationsCorresponds to variant dbSNP:rs12564525Ensembl.1
    Natural variantiVAR_044352130G → S in allele CYP4A22*4, allele CYP4A22*10, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 PublicationsCorresponds to variant dbSNP:rs2056900Ensembl.1
    Natural variantiVAR_044353152N → Y in allele CYP4A22*2, allele CYP4A22*3, allele CYP4A22*4, allele CYP4A22*5, allele CYP4A22*6, allele CYP4A22*7, allele CYP4A22*8, allele CYP4A22*9, allele CYP4A22*10, allele CYP4A22*11, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 PublicationsCorresponds to variant dbSNP:rs2056899Ensembl.1
    Natural variantiVAR_044354185V → F in allele CYP4A22*10, allele CYP4A22*11, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 1 PublicationCorresponds to variant dbSNP:rs4926581Ensembl.1
    Natural variantiVAR_044355226S → N1 PublicationCorresponds to variant dbSNP:rs35202523Ensembl.1
    Natural variantiVAR_044356230C → S1 PublicationCorresponds to variant dbSNP:rs35156123Ensembl.1
    Natural variantiVAR_044357231C → R Allele CYP4A22*2, allele CYP4A22*3, allele CYP4A22*4, allele CYP4A22*5, allele CYP4A22*6, allele CYP4A22*7, allele CYP4A22*8, allele CYP4A22*9, allele CYP4A22*10, allele CYP4A22*11, allele CYP4A22*12, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 PublicationsCorresponds to variant dbSNP:rs10789501Ensembl.1
    Natural variantiVAR_044358276K → T in allele CYP4A22*8, allele CYP4A22*11, allele CYP4A22*14 and allele CYP4A22*15. 1 PublicationCorresponds to variant dbSNP:rs6661132Ensembl.1
    Natural variantiVAR_044359428L → P in allele CYP4A22*6, allele CYP4A22*9, allele CYP4A22*10, allele CYP4A22*12, allele CYP4A22*13 and allele CYP4A22*15. 2 PublicationsCorresponds to variant dbSNP:rs2405599Ensembl.1
    Natural variantiVAR_044360491M → I1 PublicationCorresponds to variant dbSNP:rs2758714Ensembl.1
    Natural variantiVAR_044361509L → F in allele CYP4A22*7, allele CYP4A22*10, allele CYP4A22*11, allele CYP4A22*13, allele CYP4A22*14 and allele CYP4A22*15. 2 PublicationsCorresponds to variant dbSNP:rs4926600Ensembl.1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_034584356 – 519Missing in isoform 2. CuratedAdd BLAST164

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF208532 Genomic DNA Translation: AAF76722.1
    AY280371 mRNA Translation: AAQ21367.1
    AY280372 mRNA Translation: AAQ21368.1
    AL135960 Genomic DNA Translation: CAI19737.1
    AL135960 Genomic DNA Translation: CAI19738.1
    BC148248 mRNA Translation: AAI48249.1
    CCDSiCCDS30707.1 [Q5TCH4-1]
    RefSeqiNP_001010969.2, NM_001010969.3 [Q5TCH4-1]
    NP_001295031.1, NM_001308102.1
    UniGeneiHs.726474

    Genome annotation databases

    EnsembliENST00000371891; ENSP00000360958; ENSG00000162365 [Q5TCH4-1]
    GeneIDi284541
    KEGGihsa:284541
    UCSCiuc001cqv.2 human [Q5TCH4-1]

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Similar proteinsi

    Cross-referencesi

    Web resourcesi

    Cytochrome P450 Allele Nomenclature Committee

    CYP4A22 alleles

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF208532 Genomic DNA Translation: AAF76722.1
    AY280371 mRNA Translation: AAQ21367.1
    AY280372 mRNA Translation: AAQ21368.1
    AL135960 Genomic DNA Translation: CAI19737.1
    AL135960 Genomic DNA Translation: CAI19738.1
    BC148248 mRNA Translation: AAI48249.1
    CCDSiCCDS30707.1 [Q5TCH4-1]
    RefSeqiNP_001010969.2, NM_001010969.3 [Q5TCH4-1]
    NP_001295031.1, NM_001308102.1
    UniGeneiHs.726474

    3D structure databases

    ProteinModelPortaliQ5TCH4
    SMRiQ5TCH4
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi129896, 1 interactor
    STRINGi9606.ENSP00000360958

    PTM databases

    iPTMnetiQ5TCH4
    PhosphoSitePlusiQ5TCH4

    Polymorphism and mutation databases

    BioMutaiCYP4A22
    DMDMi74746067

    Proteomic databases

    PaxDbiQ5TCH4
    PeptideAtlasiQ5TCH4
    PRIDEiQ5TCH4
    ProteomicsDBi64954
    64955 [Q5TCH4-2]

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000371891; ENSP00000360958; ENSG00000162365 [Q5TCH4-1]
    GeneIDi284541
    KEGGihsa:284541
    UCSCiuc001cqv.2 human [Q5TCH4-1]

    Organism-specific databases

    CTDi284541
    EuPathDBiHostDB:ENSG00000162365.11
    GeneCardsiCYP4A22
    H-InvDBiHIX0028676
    HGNCiHGNC:20575 CYP4A22
    MIMi615341 gene
    neXtProtiNX_Q5TCH4
    OpenTargetsiENSG00000162365
    PharmGKBiPA134979692
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG0157 Eukaryota
    COG2124 LUCA
    GeneTreeiENSGT00900000140829
    HOGENOMiHOG000233833
    HOVERGENiHBG000182
    InParanoidiQ5TCH4
    KOiK07425
    PhylomeDBiQ5TCH4
    TreeFamiTF105088

    Enzyme and pathway databases

    ReactomeiR-HSA-211935 Fatty acids
    R-HSA-211958 Miscellaneous substrates
    R-HSA-211979 Eicosanoids
    R-HSA-2142691 Synthesis of Leukotrienes (LT) and Eoxins (EX)

    Miscellaneous databases

    GeneWikiiCYP4A22
    GenomeRNAii284541
    PROiPR:Q5TCH4
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000162365 Expressed in 41 organ(s), highest expression level in right lobe of liver
    ExpressionAtlasiQ5TCH4 baseline and differential
    GenevisibleiQ5TCH4 HS

    Family and domain databases

    Gene3Di1.10.630.10, 1 hit
    InterProiView protein in InterPro
    IPR001128 Cyt_P450
    IPR017972 Cyt_P450_CS
    IPR002401 Cyt_P450_E_grp-I
    IPR036396 Cyt_P450_sf
    PfamiView protein in Pfam
    PF00067 p450, 1 hit
    PRINTSiPR00463 EP450I
    PR00385 P450
    SUPFAMiSSF48264 SSF48264, 1 hit
    PROSITEiView protein in PROSITE
    PS00086 CYTOCHROME_P450, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiCP4AM_HUMAN
    AccessioniPrimary (citable) accession number: Q5TCH4
    Secondary accession number(s): Q5TCH3
    , Q6JXK7, Q6JXK8, Q9NRM4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: December 21, 2004
    Last modified: November 7, 2018
    This is version 124 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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