UniProtKB - Q5T6F0 (DCA12_HUMAN)
DDB1- and CUL4-associated factor 12
DCAF12
Functioni
Substrate-recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation (PubMed:16949367, PubMed:16964240, PubMed:29779948).
The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms (PubMed:29779948).
The DCX(DCAF12) complex specifically recognizes proteins with a diglutamate (Glu-Glu) at the C-terminus, such as MAGEA3, MAGEA6 and CCT5, leading to their ubiquitination and degradation (PubMed:29779948, PubMed:31267705).
Ubiquitination of MAGEA3, MAGEA6 by DCX(DCAF12) complex is required for starvation-induced autophagy (PubMed:31267705).
4 Publications: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- ubiquitin ligase-substrate adaptor activity Source: UniProtKB
GO - Biological processi
- regulation of autophagy Source: UniProtKB
- ubiquitin-dependent protein catabolic process via the C-end degron rule pathway Source: UniProtKB
Keywordsi
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
PathwayCommonsi | Q5T6F0 |
SignaLinki | Q5T6F0 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: DDB1- and CUL4-associated factor 12CuratedAlternative name(s): Centrosome-related protein TCC521 Publication Testis cancer centrosome-related protein1 Publication WD repeat-containing protein 40AImported |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:19911, DCAF12 |
neXtProti | NX_Q5T6F0 |
VEuPathDBi | HostDB:ENSG00000198876 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Cytoskeleton
- centrosome 1 Publication
Cytoskeleton
- centrosome Source: UniProtKB
Other locations
- Cul4-RING E3 ubiquitin ligase complex Source: UniProtKB
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, CytoskeletonPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 368 | R → A: Reduces association with DDB1. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 25853 |
OpenTargetsi | ENSG00000198876 |
PharmGKBi | PA165585735 |
Miscellaneous databases
Pharosi | Q5T6F0, Tbio |
Genetic variation databases
BioMutai | DCAF12 |
DMDMi | 74756352 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000306840 | 1 – 453 | DDB1- and CUL4-associated factor 12Add BLAST | 453 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 15 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q5T6F0 |
jPOSTi | Q5T6F0 |
MassIVEi | Q5T6F0 |
MaxQBi | Q5T6F0 |
PaxDbi | Q5T6F0 |
PeptideAtlasi | Q5T6F0 |
PRIDEi | Q5T6F0 |
ProteomicsDBi | 64578 |
PTM databases
iPTMneti | Q5T6F0 |
PhosphoSitePlusi | Q5T6F0 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000198876, Expressed in bone marrow and 218 other tissues |
ExpressionAtlasi | Q5T6F0, baseline and differential |
Genevisiblei | Q5T6F0, HS |
Organism-specific databases
HPAi | ENSG00000198876, Low tissue specificity |
Interactioni
Subunit structurei
Component of the DCX(DCAF12) E3 ubiquitin ligase complex, at least composed of CUL4 (CUL4A or CUL4B), DDB1, DCAF12 and RBX1.
3 PublicationsProtein-protein interaction databases
BioGRIDi | 117377, 30 interactors |
DIPi | DIP-48760N |
IntActi | Q5T6F0, 18 interactors |
STRINGi | 9606.ENSP00000355114 |
Miscellaneous databases
RNActi | Q5T6F0, protein |
Structurei
Secondary structure
3D structure databases
SMRi | Q5T6F0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q5T6F0 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 81 – 122 | WD 1Add BLAST | 42 | |
Repeati | 123 – 175 | WD 2Add BLAST | 53 | |
Repeati | 176 – 242 | WD 3Add BLAST | 67 | |
Repeati | 243 – 286 | WD 4Add BLAST | 44 | |
Repeati | 287 – 331 | WD 5Add BLAST | 45 | |
Repeati | 332 – 366 | WD 6Add BLAST | 35 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 34 | DisorderedSequence analysisAdd BLAST | 34 |
Sequence similaritiesi
Keywords - Domaini
Repeat, WD repeatPhylogenomic databases
eggNOGi | ENOG502QR7U, Eukaryota |
GeneTreei | ENSGT00940000158028 |
HOGENOMi | CLU_020124_1_0_1 |
InParanoidi | Q5T6F0 |
OMAi | FDMCWLD |
OrthoDBi | 885170at2759 |
PhylomeDBi | Q5T6F0 |
TreeFami | TF323731 |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
SMARTi | View protein in SMART SM00320, WD40, 4 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MARKVVSRKR KAPASPGAGS DAQGPQFGWD HSLHKRKRLP PVKRSLVYYL
60 70 80 90 100
KNREVRLQNE TSYSRVLHGY AAQQLPSLLK EREFHLGTLN KVFASQWLNH
110 120 130 140 150
RQVVCGTKCN TLFVVDVQTS QITKIPILKD REPGGVTQQG CGIHAIELNP
160 170 180 190 200
SRTLLATGGD NPNSLAIYRL PTLDPVCVGD DGHKDWIFSI AWISDTMAVS
210 220 230 240 250
GSRDGSMGLW EVTDDVLTKS DARHNVSRVP VYAHITHKAL KDIPKEDTNP
260 270 280 290 300
DNCKVRALAF NNKNKELGAV SLDGYFHLWK AENTLSKLLS TKLPYCRENV
310 320 330 340 350
CLAYGSEWSV YAVGSQAHVS FLDPRQPSYN VKSVCSRERG SGIRSVSFYE
360 370 380 390 400
HIITVGTGQG SLLFYDIRAQ RFLEERLSAC YGSKPRLAGE NLKLTTGKGW
410 420 430 440 450
LNHDETWRNY FSDIDFFPNA VYTHCYDSSG TKLFVAGGPL PSGLHGNYAG
LWS
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketX6RBJ8 | X6RBJ8_HUMAN | DDB1- and CUL4-associated factor 12 | DCAF12 | 227 | Annotation score: | ||
X6RL26 | X6RL26_HUMAN | DDB1- and CUL4-associated factor 12 | DCAF12 | 189 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 149 | N → H in BAA91106 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_035322 | 131 | R → Q1 PublicationCorresponds to variant dbSNP:rs11557154Ensembl. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ518909 mRNA Translation: ABG24246.1 AB067479 mRNA Translation: BAB67785.1 Different initiation. AK000354 mRNA Translation: BAA91106.1 Frameshift. AK292935 mRNA Translation: BAF85624.1 AL354989 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58472.1 CH471071 Genomic DNA Translation: EAW58473.1 BC008893 mRNA Translation: AAH08893.2 BC063823 mRNA Translation: AAH63823.1 AL117646 mRNA Translation: CAB56028.2 |
CCDSi | CCDS6549.1 |
PIRi | T17338 |
RefSeqi | NP_056212.1, NM_015397.3 XP_011516145.1, XM_011517843.1 |
Genome annotation databases
Ensembli | ENST00000361264; ENSP00000355114; ENSG00000198876 |
GeneIDi | 25853 |
KEGGi | hsa:25853 |
MANE-Selecti | ENST00000361264.9; ENSP00000355114.3; NM_015397.4; NP_056212.1 |
UCSCi | uc003ztt.3, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ518909 mRNA Translation: ABG24246.1 AB067479 mRNA Translation: BAB67785.1 Different initiation. AK000354 mRNA Translation: BAA91106.1 Frameshift. AK292935 mRNA Translation: BAF85624.1 AL354989 Genomic DNA No translation available. CH471071 Genomic DNA Translation: EAW58472.1 CH471071 Genomic DNA Translation: EAW58473.1 BC008893 mRNA Translation: AAH08893.2 BC063823 mRNA Translation: AAH63823.1 AL117646 mRNA Translation: CAB56028.2 |
CCDSi | CCDS6549.1 |
PIRi | T17338 |
RefSeqi | NP_056212.1, NM_015397.3 XP_011516145.1, XM_011517843.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3I7P | X-ray | 3.00 | B | 45-57 | [»] | |
SMRi | Q5T6F0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 117377, 30 interactors |
DIPi | DIP-48760N |
IntActi | Q5T6F0, 18 interactors |
STRINGi | 9606.ENSP00000355114 |
PTM databases
iPTMneti | Q5T6F0 |
PhosphoSitePlusi | Q5T6F0 |
Genetic variation databases
BioMutai | DCAF12 |
DMDMi | 74756352 |
Proteomic databases
EPDi | Q5T6F0 |
jPOSTi | Q5T6F0 |
MassIVEi | Q5T6F0 |
MaxQBi | Q5T6F0 |
PaxDbi | Q5T6F0 |
PeptideAtlasi | Q5T6F0 |
PRIDEi | Q5T6F0 |
ProteomicsDBi | 64578 |
Protocols and materials databases
Antibodypediai | 2916, 137 antibodies from 26 providers |
DNASUi | 25853 |
Genome annotation databases
Ensembli | ENST00000361264; ENSP00000355114; ENSG00000198876 |
GeneIDi | 25853 |
KEGGi | hsa:25853 |
MANE-Selecti | ENST00000361264.9; ENSP00000355114.3; NM_015397.4; NP_056212.1 |
UCSCi | uc003ztt.3, human |
Organism-specific databases
CTDi | 25853 |
DisGeNETi | 25853 |
GeneCardsi | DCAF12 |
HGNCi | HGNC:19911, DCAF12 |
HPAi | ENSG00000198876, Low tissue specificity |
neXtProti | NX_Q5T6F0 |
OpenTargetsi | ENSG00000198876 |
PharmGKBi | PA165585735 |
VEuPathDBi | HostDB:ENSG00000198876 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502QR7U, Eukaryota |
GeneTreei | ENSGT00940000158028 |
HOGENOMi | CLU_020124_1_0_1 |
InParanoidi | Q5T6F0 |
OMAi | FDMCWLD |
OrthoDBi | 885170at2759 |
PhylomeDBi | Q5T6F0 |
TreeFami | TF323731 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q5T6F0 |
SignaLinki | Q5T6F0 |
Miscellaneous databases
BioGRID-ORCSi | 25853, 15 hits in 1083 CRISPR screens |
ChiTaRSi | DCAF12, human |
EvolutionaryTracei | Q5T6F0 |
GenomeRNAii | 25853 |
Pharosi | Q5T6F0, Tbio |
PROi | PR:Q5T6F0 |
RNActi | Q5T6F0, protein |
Gene expression databases
Bgeei | ENSG00000198876, Expressed in bone marrow and 218 other tissues |
ExpressionAtlasi | Q5T6F0, baseline and differential |
Genevisiblei | Q5T6F0, HS |
Family and domain databases
Gene3Di | 2.130.10.10, 2 hits |
InterProi | View protein in InterPro IPR015943, WD40/YVTN_repeat-like_dom_sf IPR001680, WD40_repeat IPR019775, WD40_repeat_CS IPR036322, WD40_repeat_dom_sf |
SMARTi | View protein in SMART SM00320, WD40, 4 hits |
SUPFAMi | SSF50978, SSF50978, 1 hit |
PROSITEi | View protein in PROSITE PS00678, WD_REPEATS_1, 1 hit PS50082, WD_REPEATS_2, 1 hit PS50294, WD_REPEATS_REGION, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DCA12_HUMAN | |
Accessioni | Q5T6F0Primary (citable) accession number: Q5T6F0 Secondary accession number(s): A8KA70 Q9UFJ1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 2, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 23, 2022 | |
This is version 142 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families