UniProtKB - Q5T4B2 (GT253_HUMAN)
Protein
Inactive glycosyltransferase 25 family member 3
Gene
CERCAM
Organism
Homo sapiens (Human)
Status
Functioni
Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.2 Publications
Caution
Has no glucosyltransferase activity. Concerning beta-galactosyltransferase activity, the level of CERCAM could be too low to detect any activity when tested in transfected insect cells.Curated
GO - Molecular functioni
- identical protein binding Source: IntAct
GO - Biological processi
- cell adhesion Source: MGI
- leukocyte cell-cell adhesion Source: ProtInc
Keywordsi
Biological process | Cell adhesion |
Enzyme and pathway databases
PathwayCommonsi | Q5T4B2 |
Protein family/group databases
CAZyi | GT25, Glycosyltransferase Family 25 |
Names & Taxonomyi
Protein namesi | Recommended name: Inactive glycosyltransferase 25 family member 3Alternative name(s): Cerebral endothelial cell adhesion molecule |
Gene namesi | Name:CERCAM Synonyms:CEECAM1, GLT25D3, KIAA1502 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000167123.18 |
HGNCi | HGNC:23723, CERCAM |
MIMi | 616626, gene |
neXtProti | NX_Q5T4B2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum lumen PROSITE-ProRule annotation
Endoplasmic reticulum
- endoplasmic reticulum lumen Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: ProtInc
Keywords - Cellular componenti
Endoplasmic reticulumPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 440 | S → D: No effect on lack of galactosyltransferase activity in a chimeric construct with COLGALT1. Exhibits some galactosyltransferase activity; when associated with R-450; M-451; Q-452 and V-453 in a chimeric construct with COLGALT1. 1 Publication | 1 | |
Mutagenesisi | 450 | Q → R: Exhibits some galactosyltransferase activity; when associated with D-440; M-451; Q-452 and V-453 in a chimeric construct with COLGALT1. 1 Publication | 1 | |
Mutagenesisi | 451 | V → M: Exhibits some galactosyltransferase activity; when associated with D-440; R-450; Q-452 and V-453 in a chimeric construct with COLGALT1. 1 Publication | 1 | |
Mutagenesisi | 452 | N → Q: Exhibits some galactosyltransferase activity; when associated with D-440; R-450; M-451 and V-453 in a chimeric construct with COLGALT1. 1 Publication | 1 | |
Mutagenesisi | 453 | P → V: Exhibits some galactosyltransferase activity; when associated with D-440; R-450; M-451 and Q-452 in a chimeric construct with COLGALT1. 1 Publication | 1 |
Organism-specific databases
OpenTargetsi | ENSG00000167123 |
PharmGKBi | PA162382177 |
Miscellaneous databases
Pharosi | Q5T4B2, Tdark |
Polymorphism and mutation databases
BioMutai | CERCAM |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 22 | Sequence analysisAdd BLAST | 22 | |
ChainiPRO_0000309544 | 23 – 595 | Inactive glycosyltransferase 25 family member 3Add BLAST | 573 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 75 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 153 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 237 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 360 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
EPDi | Q5T4B2 |
jPOSTi | Q5T4B2 |
MassIVEi | Q5T4B2 |
MaxQBi | Q5T4B2 |
PaxDbi | Q5T4B2 |
PeptideAtlasi | Q5T4B2 |
PRIDEi | Q5T4B2 |
ProteomicsDBi | 64440 [Q5T4B2-1] 64441 [Q5T4B2-2] |
PTM databases
GlyGeni | Q5T4B2, 4 sites |
iPTMneti | Q5T4B2 |
PhosphoSitePlusi | Q5T4B2 |
Expressioni
Tissue specificityi
Ubiquitous. Highly expressed in secretory and nervous tissues.2 Publications
Gene expression databases
Bgeei | ENSG00000167123, Expressed in substantia nigra and 189 other tissues |
ExpressionAtlasi | Q5T4B2, baseline and differential |
Genevisiblei | Q5T4B2, HS |
Organism-specific databases
HPAi | ENSG00000167123, Tissue enhanced (brain) |
Interactioni
Binary interactionsi
Hide detailsQ5T4B2
GO - Molecular functioni
- identical protein binding Source: IntAct
Protein-protein interaction databases
BioGRIDi | 119332, 78 interactors |
IntActi | Q5T4B2, 54 interactors |
STRINGi | 9606.ENSP00000361929 |
Miscellaneous databases
RNActi | Q5T4B2, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 592 – 595 | Prevents secretion from ERPROSITE-ProRule annotation | 4 |
Sequence similaritiesi
Belongs to the glycosyltransferase 25 family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4179, Eukaryota |
GeneTreei | ENSGT00990000203644 |
HOGENOMi | CLU_024037_2_0_1 |
InParanoidi | Q5T4B2 |
OMAi | NRQRQGC |
OrthoDBi | 931915at2759 |
PhylomeDBi | Q5T4B2 |
TreeFami | TF313826 |
Family and domain databases
CDDi | cd06532, Glyco_transf_25, 1 hit |
InterProi | View protein in InterPro IPR002654, Glyco_trans_25 IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01755, Glyco_transf_25, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 6 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q5T4B2-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MRAARAAPLL QLLLLLGPWL EAAGVAESPL PAVVLAILAR NAEHSLPHYL
60 70 80 90 100
GALERLDYPR ARMALWCATD HNVDNTTEML QEWLAAVGDD YAAVVWRPEG
110 120 130 140 150
EPRFYPDEEG PKHWTKERHQ FLMELKQEAL TFARNWGADY ILFADTDNIL
160 170 180 190 200
TNNQTLRLLM GQGLPVVAPM LDSQTYYSNF WCGITPQGYY RRTAEYFPTK
210 220 230 240 250
NRQRRGCFRV PMVHSTFLAS LRAEGADQLA FYPPHPNYTW PFDDIIVFAY
260 270 280 290 300
ACQAAGVSVH VCNEHRYGYM NVPVKSHQGL EDERVNFIHL ILEALVDGPR
310 320 330 340 350
MQASAHVTRP SKRPSKIGFD EVFVISLARR PDRRERMLAS LWEMEISGRV
360 370 380 390 400
VDAVDGWMLN SSAIRNLGVD LLPGYQDPYS GRTLTKGEVG CFLSHYSIWE
410 420 430 440 450
EVVARGLARV LVFEDDVRFE SNFRGRLERL MEDVEAEKLS WDLIYLGRKQ
460 470 480 490 500
VNPEKETAVE GLPGLVVAGY SYWTLAYALR LAGARKLLAS QPLRRMLPVD
510 520 530 540 550
EFLPIMFDQH PNEQYKAHFW PRDLVAFSAQ PLLAAPTHYA GDAEWLSDTE
560 570 580 590
TSSPWDDDSG RLISWSGSQK TLRSPRLDLT GSSGHSLQPQ PRDEL
Computationally mapped potential isoform sequencesi
There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A087WYE2 | A0A087WYE2_HUMAN | Inactive glycosyltransferase 25 fam... | CERCAM | 517 | Annotation score: | ||
A0A087WUJ5 | A0A087WUJ5_HUMAN | Inactive glycosyltransferase 25 fam... | CERCAM | 155 | Annotation score: | ||
X6RL83 | X6RL83_HUMAN | Inactive glycosyltransferase 25 fam... | CERCAM | 218 | Annotation score: | ||
B7ZBS8 | B7ZBS8_HUMAN | Inactive glycosyltransferase 25 fam... | CERCAM | 64 | Annotation score: | ||
B7ZBS9 | B7ZBS9_HUMAN | Inactive glycosyltransferase 25 fam... | CERCAM | 79 | Annotation score: | ||
B7ZBT0 | B7ZBT0_HUMAN | Inactive glycosyltransferase 25 fam... | CERCAM | 109 | Annotation score: |
Sequence cautioni
The sequence AAH98432 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI19699 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI19700 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA96026 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC11040 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 209 | R → H in AAI19700 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 356 – 358 | GWM → AGW in AAD51367 (PubMed:10608765).Curated | 3 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_029234 | 1 – 78 | Missing in isoform 2. 3 PublicationsAdd BLAST | 78 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF177203 mRNA Translation: AAD51367.1 AK074519 mRNA Translation: BAC11036.1 AK074523 mRNA Translation: BAC11040.1 Different initiation. AL359091 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW87787.1 CH471090 Genomic DNA Translation: EAW87789.1 BC011811 mRNA Translation: AAH11811.2 BC098432 mRNA Translation: AAH98432.2 Different initiation. BC108698 mRNA Translation: AAI08699.1 BC119698 mRNA Translation: AAI19699.1 Different initiation. BC119699 mRNA Translation: AAI19700.1 Different initiation. AB040935 mRNA Translation: BAA96026.1 Different initiation. |
CCDSi | CCDS6901.2 [Q5T4B2-1] CCDS69675.1 [Q5T4B2-2] |
RefSeqi | NP_001273689.1, NM_001286760.1 [Q5T4B2-2] NP_057258.3, NM_016174.4 [Q5T4B2-1] XP_005252092.1, XM_005252035.1 [Q5T4B2-1] XP_011517064.1, XM_011518762.1 [Q5T4B2-1] XP_011517065.1, XM_011518763.2 [Q5T4B2-2] XP_016870283.1, XM_017014794.1 [Q5T4B2-1] |
Genome annotation databases
Ensembli | ENST00000372838; ENSP00000361929; ENSG00000167123 [Q5T4B2-1] ENST00000372842; ENSP00000361933; ENSG00000167123 [Q5T4B2-2] |
GeneIDi | 51148 |
KEGGi | hsa:51148 |
UCSCi | uc004buz.5, human [Q5T4B2-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF177203 mRNA Translation: AAD51367.1 AK074519 mRNA Translation: BAC11036.1 AK074523 mRNA Translation: BAC11040.1 Different initiation. AL359091 Genomic DNA No translation available. CH471090 Genomic DNA Translation: EAW87787.1 CH471090 Genomic DNA Translation: EAW87789.1 BC011811 mRNA Translation: AAH11811.2 BC098432 mRNA Translation: AAH98432.2 Different initiation. BC108698 mRNA Translation: AAI08699.1 BC119698 mRNA Translation: AAI19699.1 Different initiation. BC119699 mRNA Translation: AAI19700.1 Different initiation. AB040935 mRNA Translation: BAA96026.1 Different initiation. |
CCDSi | CCDS6901.2 [Q5T4B2-1] CCDS69675.1 [Q5T4B2-2] |
RefSeqi | NP_001273689.1, NM_001286760.1 [Q5T4B2-2] NP_057258.3, NM_016174.4 [Q5T4B2-1] XP_005252092.1, XM_005252035.1 [Q5T4B2-1] XP_011517064.1, XM_011518762.1 [Q5T4B2-1] XP_011517065.1, XM_011518763.2 [Q5T4B2-2] XP_016870283.1, XM_017014794.1 [Q5T4B2-1] |
3D structure databases
SMRi | Q5T4B2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 119332, 78 interactors |
IntActi | Q5T4B2, 54 interactors |
STRINGi | 9606.ENSP00000361929 |
Protein family/group databases
CAZyi | GT25, Glycosyltransferase Family 25 |
PTM databases
GlyGeni | Q5T4B2, 4 sites |
iPTMneti | Q5T4B2 |
PhosphoSitePlusi | Q5T4B2 |
Polymorphism and mutation databases
BioMutai | CERCAM |
Proteomic databases
EPDi | Q5T4B2 |
jPOSTi | Q5T4B2 |
MassIVEi | Q5T4B2 |
MaxQBi | Q5T4B2 |
PaxDbi | Q5T4B2 |
PeptideAtlasi | Q5T4B2 |
PRIDEi | Q5T4B2 |
ProteomicsDBi | 64440 [Q5T4B2-1] 64441 [Q5T4B2-2] |
Protocols and materials databases
Antibodypediai | 17470, 109 antibodies |
DNASUi | 51148 |
Genome annotation databases
Ensembli | ENST00000372838; ENSP00000361929; ENSG00000167123 [Q5T4B2-1] ENST00000372842; ENSP00000361933; ENSG00000167123 [Q5T4B2-2] |
GeneIDi | 51148 |
KEGGi | hsa:51148 |
UCSCi | uc004buz.5, human [Q5T4B2-1] |
Organism-specific databases
CTDi | 51148 |
EuPathDBi | HostDB:ENSG00000167123.18 |
GeneCardsi | CERCAM |
HGNCi | HGNC:23723, CERCAM |
HPAi | ENSG00000167123, Tissue enhanced (brain) |
MIMi | 616626, gene |
neXtProti | NX_Q5T4B2 |
OpenTargetsi | ENSG00000167123 |
PharmGKBi | PA162382177 |
HUGEi | Search... |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4179, Eukaryota |
GeneTreei | ENSGT00990000203644 |
HOGENOMi | CLU_024037_2_0_1 |
InParanoidi | Q5T4B2 |
OMAi | NRQRQGC |
OrthoDBi | 931915at2759 |
PhylomeDBi | Q5T4B2 |
TreeFami | TF313826 |
Enzyme and pathway databases
PathwayCommonsi | Q5T4B2 |
Miscellaneous databases
BioGRID-ORCSi | 51148, 14 hits in 843 CRISPR screens |
ChiTaRSi | CERCAM, human |
GenomeRNAii | 51148 |
Pharosi | Q5T4B2, Tdark |
PROi | PR:Q5T4B2 |
RNActi | Q5T4B2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000167123, Expressed in substantia nigra and 189 other tissues |
ExpressionAtlasi | Q5T4B2, baseline and differential |
Genevisiblei | Q5T4B2, HS |
Family and domain databases
CDDi | cd06532, Glyco_transf_25, 1 hit |
InterProi | View protein in InterPro IPR002654, Glyco_trans_25 IPR029044, Nucleotide-diphossugar_trans |
Pfami | View protein in Pfam PF01755, Glyco_transf_25, 1 hit |
SUPFAMi | SSF53448, SSF53448, 1 hit |
PROSITEi | View protein in PROSITE PS00014, ER_TARGET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GT253_HUMAN | |
Accessioni | Q5T4B2Primary (citable) accession number: Q5T4B2 Secondary accession number(s): A7MD00 Q9UMW5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families