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Protein

Inactive glycosyltransferase 25 family member 3

Gene

CERCAM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable cell adhesion protein involved in leukocyte transmigration across the blood-brain barrier. Does not express any beta-galactosyltransferase activity in vitro.2 Publications

Caution

Has no glucosyltransferase activity. Concerning beta-galactosyltransferase activity, the level of CERCAM could be too low to detect any activity when tested in transfected insect cells.Curated

GO - Biological processi

  • cell adhesion Source: MGI
  • leukocyte cell-cell adhesion Source: ProtInc

Keywordsi

Biological processCell adhesion

Protein family/group databases

CAZyiGT25 Glycosyltransferase Family 25

Names & Taxonomyi

Protein namesi
Recommended name:
Inactive glycosyltransferase 25 family member 3
Alternative name(s):
Cerebral endothelial cell adhesion molecule
Gene namesi
Name:CERCAM
Synonyms:CEECAM1, GLT25D3, KIAA1502
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000167123.18
HGNCiHGNC:23723 CERCAM
MIMi616626 gene
neXtProtiNX_Q5T4B2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi440S → D: No effect on lack of galactosyltransferase activity in a chimeric construct with COLGALT1. Exhibits some galactosyltransferase activity; when associated with R-450; M-451; Q-452 and V-453 in a chimeric construct with COLGALT1. 1 Publication1
Mutagenesisi450Q → R: Exhibits some galactosyltransferase activity; when associated with D-440; M-451; Q-452 and V-453 in a chimeric construct with COLGALT1. 1 Publication1
Mutagenesisi451V → M: Exhibits some galactosyltransferase activity; when associated with D-440; R-450; Q-452 and V-453 in a chimeric construct with COLGALT1. 1 Publication1
Mutagenesisi452N → Q: Exhibits some galactosyltransferase activity; when associated with D-440; R-450; M-451 and V-453 in a chimeric construct with COLGALT1. 1 Publication1
Mutagenesisi453P → V: Exhibits some galactosyltransferase activity; when associated with D-440; R-450; M-451 and Q-452 in a chimeric construct with COLGALT1. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000167123
PharmGKBiPA162382177

Polymorphism and mutation databases

BioMutaiCERCAM

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000030954423 – 595Inactive glycosyltransferase 25 family member 3Add BLAST573

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi153N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi237N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi360N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ5T4B2
MaxQBiQ5T4B2
PaxDbiQ5T4B2
PeptideAtlasiQ5T4B2
PRIDEiQ5T4B2
ProteomicsDBi64440
64441 [Q5T4B2-2]

PTM databases

iPTMnetiQ5T4B2
PhosphoSitePlusiQ5T4B2

Expressioni

Tissue specificityi

Ubiquitous. Highly expressed in secretory and nervous tissues.2 Publications

Gene expression databases

BgeeiENSG00000167123
CleanExiHS_CERCAM
ExpressionAtlasiQ5T4B2 baseline and differential
GenevisibleiQ5T4B2 HS

Organism-specific databases

HPAiHPA021657
HPA051595

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi119332, 43 interactors
IntActiQ5T4B2, 28 interactors
STRINGi9606.ENSP00000361929

Structurei

3D structure databases

ProteinModelPortaliQ5T4B2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi592 – 595Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Belongs to the glycosyltransferase 25 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4179 Eukaryota
ENOG410XQQN LUCA
GeneTreeiENSGT00550000074427
HOVERGENiHBG058097
InParanoidiQ5T4B2
OMAiEYFPTKN
OrthoDBiEOG091G0SKW
PhylomeDBiQ5T4B2
TreeFamiTF313826

Family and domain databases

CDDicd06532 Glyco_transf_25, 1 hit
InterProiView protein in InterPro
IPR002654 Glyco_trans_25
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01755 Glyco_transf_25, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q5T4B2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRAARAAPLL QLLLLLGPWL EAAGVAESPL PAVVLAILAR NAEHSLPHYL
60 70 80 90 100
GALERLDYPR ARMALWCATD HNVDNTTEML QEWLAAVGDD YAAVVWRPEG
110 120 130 140 150
EPRFYPDEEG PKHWTKERHQ FLMELKQEAL TFARNWGADY ILFADTDNIL
160 170 180 190 200
TNNQTLRLLM GQGLPVVAPM LDSQTYYSNF WCGITPQGYY RRTAEYFPTK
210 220 230 240 250
NRQRRGCFRV PMVHSTFLAS LRAEGADQLA FYPPHPNYTW PFDDIIVFAY
260 270 280 290 300
ACQAAGVSVH VCNEHRYGYM NVPVKSHQGL EDERVNFIHL ILEALVDGPR
310 320 330 340 350
MQASAHVTRP SKRPSKIGFD EVFVISLARR PDRRERMLAS LWEMEISGRV
360 370 380 390 400
VDAVDGWMLN SSAIRNLGVD LLPGYQDPYS GRTLTKGEVG CFLSHYSIWE
410 420 430 440 450
EVVARGLARV LVFEDDVRFE SNFRGRLERL MEDVEAEKLS WDLIYLGRKQ
460 470 480 490 500
VNPEKETAVE GLPGLVVAGY SYWTLAYALR LAGARKLLAS QPLRRMLPVD
510 520 530 540 550
EFLPIMFDQH PNEQYKAHFW PRDLVAFSAQ PLLAAPTHYA GDAEWLSDTE
560 570 580 590
TSSPWDDDSG RLISWSGSQK TLRSPRLDLT GSSGHSLQPQ PRDEL
Length:595
Mass (Da):67,592
Last modified:December 21, 2004 - v1
Checksum:iFC2157AD8C7AC558
GO
Isoform 2 (identifier: Q5T4B2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-78: Missing.

Show »
Length:517
Mass (Da):59,100
Checksum:i8E8C443064488AE7
GO

Sequence cautioni

The sequence AAH98432 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI19699 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAI19700 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA96026 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC11040 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAI13495 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti209R → H in AAI19700 (PubMed:15489334).Curated1
Sequence conflicti356 – 358GWM → AGW in AAD51367 (PubMed:10608765).Curated3

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0292341 – 78Missing in isoform 2. 3 PublicationsAdd BLAST78

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF177203 mRNA Translation: AAD51367.1
AK074519 mRNA Translation: BAC11036.1
AK074523 mRNA Translation: BAC11040.1 Different initiation.
AL359091 Genomic DNA Translation: CAI13494.1
AL359091 Genomic DNA Translation: CAI13495.1 Sequence problems.
AL359091 Genomic DNA Translation: CAI13496.1
CH471090 Genomic DNA Translation: EAW87787.1
CH471090 Genomic DNA Translation: EAW87789.1
BC011811 mRNA Translation: AAH11811.2
BC098432 mRNA Translation: AAH98432.2 Different initiation.
BC108698 mRNA Translation: AAI08699.1
BC119698 mRNA Translation: AAI19699.1 Different initiation.
BC119699 mRNA Translation: AAI19700.1 Different initiation.
AB040935 mRNA Translation: BAA96026.1 Different initiation.
CCDSiCCDS6901.2 [Q5T4B2-1]
CCDS69675.1 [Q5T4B2-2]
RefSeqiNP_001273689.1, NM_001286760.1 [Q5T4B2-2]
NP_057258.3, NM_016174.4 [Q5T4B2-1]
XP_005252092.1, XM_005252035.1 [Q5T4B2-1]
XP_011517064.1, XM_011518762.1 [Q5T4B2-1]
XP_011517065.1, XM_011518763.2 [Q5T4B2-2]
XP_016870283.1, XM_017014794.1 [Q5T4B2-1]
UniGeneiHs.495230

Genome annotation databases

EnsembliENST00000372838; ENSP00000361929; ENSG00000167123 [Q5T4B2-1]
ENST00000372842; ENSP00000361933; ENSG00000167123 [Q5T4B2-2]
GeneIDi51148
KEGGihsa:51148
UCSCiuc004buz.5 human [Q5T4B2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiGT253_HUMAN
AccessioniPrimary (citable) accession number: Q5T4B2
Secondary accession number(s): A7MD00
, C4AMA2, Q0VDF3, Q2VPJ4, Q4KMP2, Q5T4B1, Q8N107, Q96EZ5, Q9P226, Q9UMW5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 21, 2004
Last modified: June 20, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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