UniProtKB - Q5T197 (DCST1_HUMAN)
Protein
E3 ubiquitin-protein ligase DCST1
Gene
DCST1
Organism
Homo sapiens (Human)
Status
Functioni
E3 ubiquitin-protein ligase which mediates 'Lys-48'-linked ubiquitination of STAT2 and induces its proteosomal degradation thereby negatively regulating type-I-interferon signaling.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 646 – 685 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 40 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- ubiquitin protein ligase activity Source: UniProtKB
GO - Biological processi
- innate immune response Source: UniProtKB-KW
- negative regulation of type I interferon-mediated signaling pathway Source: UniProtKB
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Transferase |
Biological process | Immunity, Innate immunity, Ubl conjugation pathway |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
PathwayCommonsi | Q5T197 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: E3 ubiquitin-protein ligase DCST1Curated (EC:2.3.2.271 Publication)Alternative name(s): DC-STAMP domain-containing protein 1 RING-type E3 ubiquitin transferaseCurated |
Gene namesi | Name:DCST1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:26539, DCST1 |
neXtProti | NX_Q5T197 |
VEuPathDBi | HostDB:ENSG00000163357.10 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 48 | CytoplasmicSequence analysisAdd BLAST | 48 | |
Transmembranei | 49 – 69 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 70 – 79 | ExtracellularSequence analysis | 10 | |
Transmembranei | 80 – 100 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 101 – 115 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 116 – 136 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 137 – 372 | ExtracellularSequence analysisAdd BLAST | 236 | |
Transmembranei | 373 – 393 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 394 – 463 | CytoplasmicSequence analysisAdd BLAST | 70 | |
Transmembranei | 464 – 484 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 485 – 553 | ExtracellularSequence analysisAdd BLAST | 69 | |
Transmembranei | 554 – 574 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 575 – 706 | CytoplasmicSequence analysisAdd BLAST | 132 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 149095 |
OpenTargetsi | ENSG00000163357 |
PharmGKBi | PA142672005 |
Miscellaneous databases
Pharosi | Q5T197, Tdark |
Genetic variation databases
BioMutai | DCST1 |
DMDMi | 74756229 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000278824 | 1 – 706 | E3 ubiquitin-protein ligase DCST1Add BLAST | 706 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 156 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 189 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 321 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 349 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 525 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
GlycoproteinProteomic databases
MassIVEi | Q5T197 |
PaxDbi | Q5T197 |
PeptideAtlasi | Q5T197 |
PRIDEi | Q5T197 |
ProteomicsDBi | 20607 64248 [Q5T197-1] 64249 [Q5T197-2] |
PTM databases
GlyGeni | Q5T197, 5 sites |
iPTMneti | Q5T197 |
PhosphoSitePlusi | Q5T197 |
Expressioni
Inductioni
Induced by IFNB1.1 Publication
Gene expression databases
Bgeei | ENSG00000163357, Expressed in skin of abdomen and 94 other tissues |
ExpressionAtlasi | Q5T197, baseline and differential |
Genevisiblei | Q5T197, HS |
Organism-specific databases
HPAi | ENSG00000163357, Group enriched (retina, skin, testis) |
Interactioni
Subunit structurei
Interacts with STAT2; the interaction results in STAT2 'Lys-48'-linked ubiquitination leading to its proteasomal degradation.
1 PublicationProtein-protein interaction databases
BioGRIDi | 127192, 4 interactors |
IntActi | Q5T197, 2 interactors |
STRINGi | 9606.ENSP00000295542 |
Miscellaneous databases
RNActi | Q5T197, protein |
Family & Domainsi
Domaini
The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity.1 Publication
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 646 – 685 | RING-type; degeneratePROSITE-ProRule annotationAdd BLAST | 40 |
Keywords - Domaini
Transmembrane, Transmembrane helix, Zinc-fingerPhylogenomic databases
eggNOGi | KOG3726, Eukaryota |
GeneTreei | ENSGT00940000153269 |
HOGENOMi | CLU_015030_1_1_1 |
InParanoidi | Q5T197 |
OMAi | MAKPFTN |
PhylomeDBi | Q5T197 |
TreeFami | TF318254 |
Family and domain databases
InterProi | View protein in InterPro IPR012858, DC_STAMP-like IPR001841, Znf_RING |
Pfami | View protein in Pfam PF07782, DC_STAMP, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q5T197-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDIKHHQNGT RGQRRKQPHT TVQRLLTWGL PVSCSWFLWR QPGEFPVTAL
60 70 80 90 100
LLGAGAGGLL AIGLFQLLVN PMNIYEEQKI MFLYSLVGLG AMGWGTSPHI
110 120 130 140 150
RCASLLLVPK MLGKEGRLFV LGYALAAIYV GPVANLRHNL NNVIASLGCT
160 170 180 190 200
VELQINNTRA AWRISTAPLR AMFKDLLSSK ELLRAETRNI SATFEDLDAQ
210 220 230 240 250
VNSETGYTPE DTMDSGETAQ GREARQAPAS RLHLSTQKMY ELKTKLRCSY
260 270 280 290 300
VVNQAILSCR RWFDRKHEQC MKHIWVPLLT HLLCLPMKFK FFCGIAKVME
310 320 330 340 350
VWCRNRIPVE GNFGQTYDSL NQSIRGLDGE FSANIDFKEE KQAGVLGLNT
360 370 380 390 400
SWERVSTEVR DYVYRQEARL EWALGLLHVL LSCTFLLVLH ASFSYMDSYN
410 420 430 440 450
HDIRFDNIYI STYFCQIDDR RKKLGKRTLL PLRKAEEKTV IFPCKPTIQA
460 470 480 490 500
SEMSNVVREL LETLPILLLL VVLCGLDWAL YSIFDTIRHH SFLQYSFRSS
510 520 530 540 550
HKLEVKVGGD SMLARLLRKT IGALNTSSET VMESNNMPCL PQPVGLDARA
560 570 580 590 600
YWRAAVPIGL LVCLCLLQAF GYRLRRVIAA FYFPKREKKR ILFLYNDLLK
610 620 630 640 650
KRAAFTKLRR AAILRRERQQ KAPRHPLADI LHRGCPLLRR WLCRRCVVCQ
660 670 680 690 700
APETPESYVC RTLDCEAVYC WSCWDDMRQR CPVCTPREEL SSSAFSDSND
DTAYAG
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketE9PJX3 | E9PJX3_HUMAN | E3 ubiquitin-protein ligase DCST1 | DCST1 | 528 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 467 | L → P in BAB71440 (PubMed:14702039).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_056859 | 158 | T → I. Corresponds to variant dbSNP:rs9427170EnsemblClinVar. | 1 | |
Natural variantiVAR_030868 | 512 | M → L2 PublicationsCorresponds to variant dbSNP:rs11264300Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_045619 | 63 – 87 | Missing in isoform 3. 1 PublicationAdd BLAST | 25 | |
Alternative sequenceiVSP_023406 | 624 – 664 | RHPLA…CRTLD → VFTEPQFLPLKNGKTGEICK ACSLEISLEWWSGVGLRCQR A in isoform 2. 1 PublicationAdd BLAST | 41 | |
Alternative sequenceiVSP_023407 | 665 – 706 | Missing in isoform 2. 1 PublicationAdd BLAST | 42 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK057347 mRNA Translation: BAB71440.1 AK301876 mRNA Translation: BAG63312.1 AL451085 Genomic DNA No translation available. BC064844 mRNA Translation: AAH64844.1 AF314227 Genomic DNA Translation: AAS46286.1 |
CCDSi | CCDS1083.1 [Q5T197-1] CCDS44235.1 [Q5T197-3] |
RefSeqi | NP_001137159.1, NM_001143687.2 [Q5T197-3] NP_689707.2, NM_152494.3 [Q5T197-1] |
Genome annotation databases
Ensembli | ENST00000295542; ENSP00000295542; ENSG00000163357 [Q5T197-1] ENST00000368419; ENSP00000357404; ENSG00000163357 [Q5T197-2] ENST00000423025; ENSP00000387369; ENSG00000163357 [Q5T197-3] |
GeneIDi | 149095 |
KEGGi | hsa:149095 |
UCSCi | uc001fgn.3, human [Q5T197-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK057347 mRNA Translation: BAB71440.1 AK301876 mRNA Translation: BAG63312.1 AL451085 Genomic DNA No translation available. BC064844 mRNA Translation: AAH64844.1 AF314227 Genomic DNA Translation: AAS46286.1 |
CCDSi | CCDS1083.1 [Q5T197-1] CCDS44235.1 [Q5T197-3] |
RefSeqi | NP_001137159.1, NM_001143687.2 [Q5T197-3] NP_689707.2, NM_152494.3 [Q5T197-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 127192, 4 interactors |
IntActi | Q5T197, 2 interactors |
STRINGi | 9606.ENSP00000295542 |
PTM databases
GlyGeni | Q5T197, 5 sites |
iPTMneti | Q5T197 |
PhosphoSitePlusi | Q5T197 |
Genetic variation databases
BioMutai | DCST1 |
DMDMi | 74756229 |
Proteomic databases
MassIVEi | Q5T197 |
PaxDbi | Q5T197 |
PeptideAtlasi | Q5T197 |
PRIDEi | Q5T197 |
ProteomicsDBi | 20607 64248 [Q5T197-1] 64249 [Q5T197-2] |
Protocols and materials databases
Antibodypediai | 20409, 86 antibodies |
Genome annotation databases
Ensembli | ENST00000295542; ENSP00000295542; ENSG00000163357 [Q5T197-1] ENST00000368419; ENSP00000357404; ENSG00000163357 [Q5T197-2] ENST00000423025; ENSP00000387369; ENSG00000163357 [Q5T197-3] |
GeneIDi | 149095 |
KEGGi | hsa:149095 |
UCSCi | uc001fgn.3, human [Q5T197-1] |
Organism-specific databases
CTDi | 149095 |
DisGeNETi | 149095 |
GeneCardsi | DCST1 |
HGNCi | HGNC:26539, DCST1 |
HPAi | ENSG00000163357, Group enriched (retina, skin, testis) |
neXtProti | NX_Q5T197 |
OpenTargetsi | ENSG00000163357 |
PharmGKBi | PA142672005 |
VEuPathDBi | HostDB:ENSG00000163357.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3726, Eukaryota |
GeneTreei | ENSGT00940000153269 |
HOGENOMi | CLU_015030_1_1_1 |
InParanoidi | Q5T197 |
OMAi | MAKPFTN |
PhylomeDBi | Q5T197 |
TreeFami | TF318254 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
PathwayCommonsi | Q5T197 |
Miscellaneous databases
BioGRID-ORCSi | 149095, 4 hits in 873 CRISPR screens |
GenomeRNAii | 149095 |
Pharosi | Q5T197, Tdark |
PROi | PR:Q5T197 |
RNActi | Q5T197, protein |
Gene expression databases
Bgeei | ENSG00000163357, Expressed in skin of abdomen and 94 other tissues |
ExpressionAtlasi | Q5T197, baseline and differential |
Genevisiblei | Q5T197, HS |
Family and domain databases
InterProi | View protein in InterPro IPR012858, DC_STAMP-like IPR001841, Znf_RING |
Pfami | View protein in Pfam PF07782, DC_STAMP, 1 hit |
PROSITEi | View protein in PROSITE PS50089, ZF_RING_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DCST1_HUMAN | |
Accessioni | Q5T197Primary (citable) accession number: Q5T197 Secondary accession number(s): B4DXA0 Q96M70 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 6, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 10, 2021 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - PATHWAY comments
Index of metabolic and biosynthesis pathways