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Protein

Formin-binding protein 1-like

Gene

FNBP1L

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. May bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. Actin polymerization may promote the fission of membrane tubules to form endocytic vesicles. Essential for autophagy of intracellular bacterial pathogens.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei165Mediates end-to-end attachment of dimersBy similarity1

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • GTPase binding Source: UniProtKB
  • lipid binding Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Biological processAutophagy, Endocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Formin-binding protein 1-like
Alternative name(s):
Transducer of Cdc42-dependent actin assembly protein 1
Short name:
Toca-1
Gene namesi
Name:FNBP1L
Synonyms:C1orf39, TOCA1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000137942.16
HGNCiHGNC:20851 FNBP1L
MIMi608848 gene
neXtProtiNX_Q5T0N5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi441 – 443MGD → IST: Impairs interaction with CDC42 and reduces CDC42-induced actin assembly. 1 Publication3
Mutagenesisi576W → K: Impairs interaction with WASL and reduces CDC42-induced actin assembly. 1 Publication1

Organism-specific databases

DisGeNETi54874
OpenTargetsiENSG00000137942
PharmGKBiPA128394675

Polymorphism and mutation databases

BioMutaiFNBP1L
DMDMi118572313

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002614341 – 605Formin-binding protein 1-likeAdd BLAST605

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei295PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1
Modified residuei501PhosphoserineCombined sources1
Modified residuei505PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ5T0N5
MaxQBiQ5T0N5
PaxDbiQ5T0N5
PeptideAtlasiQ5T0N5
PRIDEiQ5T0N5
ProteomicsDBi64183
64184 [Q5T0N5-2]
64185 [Q5T0N5-3]
64186 [Q5T0N5-4]
64187 [Q5T0N5-5]

PTM databases

iPTMnetiQ5T0N5
PhosphoSitePlusiQ5T0N5

Expressioni

Gene expression databases

BgeeiENSG00000137942 Expressed in 228 organ(s), highest expression level in forebrain
CleanExiHS_FNBP1L
ExpressionAtlasiQ5T0N5 baseline and differential
GenevisibleiQ5T0N5 HS

Organism-specific databases

HPAiHPA065273

Interactioni

Subunit structurei

Homodimerizes, the dimers can polymerize end-to-end to form filamentous structures (By similarity). Interacts with GTP-bound CDC42 (PubMed:15260990). Interacts with DAAM1, DIAPH1, DIAPH2, DNM1, DNM2 and WASL/N-WASP (PubMed:16326391, PubMed:16630611, PubMed:16418535). Interacts with ATG3 (PubMed:19342671). Interacts (via SH3 domain) with ABI1, WASF2, CDC42 and WIPF1 (PubMed:19798448).By similarity6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DAAM1Q9Y4D12EBI-714058,EBI-2817289

GO - Molecular functioni

Protein-protein interaction databases

BioGridi120222, 27 interactors
IntActiQ5T0N5, 13 interactors
MINTiQ5T0N5
STRINGi9606.ENSP00000271234

Structurei

3D structure databases

ProteinModelPortaliQ5T0N5
SMRiQ5T0N5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 263F-BARPROSITE-ProRule annotationAdd BLAST263
Domaini397 – 474REM-1PROSITE-ProRule annotationAdd BLAST78
Domaini538 – 599SH3PROSITE-ProRule annotationAdd BLAST62

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni245 – 535Interaction with CDC42Add BLAST291
Regioni522 – 605Interaction with DNM11 PublicationAdd BLAST84
Regioni541 – 605Interaction with DAAM1, DIAPH1 and DIAPH21 PublicationAdd BLAST65
Regioni541 – 597Interaction with DNM2 and WASL1 PublicationAdd BLAST57

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili66 – 258By similarityAdd BLAST193
Coiled coili392 – 484By similarityAdd BLAST93

Domaini

The F-BAR domain binds the phospholipid membrane with its concave surface. The end-to-end polymerization of dimers of these domains provides a curved surface that fits best membranes with around 600 A diameter, and may drive tubulation (By similarity).By similarity

Sequence similaritiesi

Belongs to the FNBP1 family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiENOG410INNW Eukaryota
ENOG410XS31 LUCA
GeneTreeiENSGT00510000046403
HOGENOMiHOG000231767
HOVERGENiHBG002489
InParanoidiQ5T0N5
KOiK20121
PhylomeDBiQ5T0N5
TreeFamiTF351162

Family and domain databases

CDDicd07675 F-BAR_FNBP1L, 1 hit
cd12072 SH3_FNBP1L, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR030116 FNBP1L
IPR035494 FNBP1L_F-BAR
IPR035493 FNBP1L_SH3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43990:SF3 PTHR43990:SF3, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS51860 REM_1, 1 hit
PS50002 SH3, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5T0N5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSWGTELWDQ FDSLDKHTQW GIDFLERYAK FVKERIEIEQ NYAKQLRNLV
60 70 80 90 100
KKYCPKRSSK DEEPRFTSCV AFFNILNELN DYAGQREVVA EEMAHRVYGE
110 120 130 140 150
LMRYAHDLKT ERKMHLQEGR KAQQYLDMCW KQMDNSKKKF ERECREAEKA
160 170 180 190 200
QQSYERLDND TNATKADVEK AKQQLNLRTH MADENKNEYA AQLQNFNGEQ
210 220 230 240 250
HKHFYVVIPQ IYKQLQEMDE RRTIKLSECY RGFADSERKV IPIISKCLEG
260 270 280 290 300
MILAAKSVDE RRDSQMVVDS FKSGFEPPGD FPFEDYSQHI YRTISDGTIS
310 320 330 340 350
ASKQESGKMD AKTTVGKAKG KLWLFGKKPK PQSPPLTPTS LFTSSTPNGS
360 370 380 390 400
QFLTFSIEPV HYCMNEIKTG KPRIPSFRSL KRGWSVKMGP ALEDFSHLPP
410 420 430 440 450
EQRRKKLQQR IDELNRELQK ESDQKDALNK MKDVYEKNPQ MGDPGSLQPK
460 470 480 490 500
LAETMNNIDR LRMEIHKNEA WLSEVEGKTG GRGDRRHSSD INHLVTQGRE
510 520 530 540 550
SPEGSYTDDA NQEVRGPPQQ HGHHNEFDDE FEDDDPLPAI GHCKAIYPFD
560 570 580 590 600
GHNEGTLAMK EGEVLYIIEE DKGDGWTRAR RQNGEEGYVP TSYIDVTLEK

NSKGS
Note: Gene prediction based on EST data.
Length:605
Mass (Da):70,065
Last modified:July 9, 2014 - v3
Checksum:i1D9237185C3006ED
GO
Isoform 2 (identifier: Q5T0N5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     384-388: Missing.

Note: Gene prediction based on EST data.
Show »
Length:600
Mass (Da):69,434
Checksum:i1C2DDFB3A3A5F0B3
GO
Isoform 3 (identifier: Q5T0N5-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     331-388: Missing.

Show »
Length:547
Mass (Da):63,572
Checksum:i416101498FF99567
GO
Isoform 4 (identifier: Q5T0N5-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     331-388: Missing.
     605-605: S → AVTYI

Note: Gene prediction based on EST data.
Show »
Length:551
Mass (Da):64,033
Checksum:iB14EB59CE9010149
GO
Isoform 5 (identifier: Q5T0N5-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     384-388: Missing.
     605-605: S → AVTYI

Note: Gene prediction based on EST data. No experimental confirmation available.
Show »
Length:604
Mass (Da):69,894
Checksum:i8C72A845744DDFB3
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R347S4R347_HUMAN
Formin-binding protein 1-like
FNBP1L
609Annotation score:
A0A075B6Q2A0A075B6Q2_HUMAN
Formin-binding protein 1-like
FNBP1L
451Annotation score:

Sequence cautioni

The sequence BAA91051 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_021709331 – 388Missing in isoform 3 and isoform 4. 3 PublicationsAdd BLAST58
Alternative sequenceiVSP_021710384 – 388Missing in isoform 2 and isoform 5. Curated5
Alternative sequenceiVSP_021711605S → AVTYI in isoform 4 and isoform 5. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY514449 mRNA Translation: AAR98814.1
AC095034 Genomic DNA No translation available.
AL109613 Genomic DNA No translation available.
AL512651 Genomic DNA No translation available.
BC062477 mRNA Translation: AAH62477.1
BC074891 mRNA Translation: AAH74891.1
BC074892 mRNA Translation: AAH74892.1
AK000282 mRNA Translation: BAA91051.1 Different initiation.
CCDSiCCDS53343.1 [Q5T0N5-1]
CCDS53344.1 [Q5T0N5-4]
CCDS60192.1 [Q5T0N5-3]
RefSeqiNP_001020119.1, NM_001024948.2 [Q5T0N5-4]
NP_001157945.1, NM_001164473.2 [Q5T0N5-1]
NP_060207.2, NM_017737.4 [Q5T0N5-3]
UniGeneiHs.134060

Genome annotation databases

EnsembliENST00000260506; ENSP00000260506; ENSG00000137942 [Q5T0N5-4]
ENST00000271234; ENSP00000271234; ENSG00000137942 [Q5T0N5-1]
ENST00000370253; ENSP00000359275; ENSG00000137942 [Q5T0N5-3]
GeneIDi54874
KEGGihsa:54874
UCSCiuc001dpv.4 human [Q5T0N5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY514449 mRNA Translation: AAR98814.1
AC095034 Genomic DNA No translation available.
AL109613 Genomic DNA No translation available.
AL512651 Genomic DNA No translation available.
BC062477 mRNA Translation: AAH62477.1
BC074891 mRNA Translation: AAH74891.1
BC074892 mRNA Translation: AAH74892.1
AK000282 mRNA Translation: BAA91051.1 Different initiation.
CCDSiCCDS53343.1 [Q5T0N5-1]
CCDS53344.1 [Q5T0N5-4]
CCDS60192.1 [Q5T0N5-3]
RefSeqiNP_001020119.1, NM_001024948.2 [Q5T0N5-4]
NP_001157945.1, NM_001164473.2 [Q5T0N5-1]
NP_060207.2, NM_017737.4 [Q5T0N5-3]
UniGeneiHs.134060

3D structure databases

ProteinModelPortaliQ5T0N5
SMRiQ5T0N5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120222, 27 interactors
IntActiQ5T0N5, 13 interactors
MINTiQ5T0N5
STRINGi9606.ENSP00000271234

PTM databases

iPTMnetiQ5T0N5
PhosphoSitePlusiQ5T0N5

Polymorphism and mutation databases

BioMutaiFNBP1L
DMDMi118572313

Proteomic databases

EPDiQ5T0N5
MaxQBiQ5T0N5
PaxDbiQ5T0N5
PeptideAtlasiQ5T0N5
PRIDEiQ5T0N5
ProteomicsDBi64183
64184 [Q5T0N5-2]
64185 [Q5T0N5-3]
64186 [Q5T0N5-4]
64187 [Q5T0N5-5]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000260506; ENSP00000260506; ENSG00000137942 [Q5T0N5-4]
ENST00000271234; ENSP00000271234; ENSG00000137942 [Q5T0N5-1]
ENST00000370253; ENSP00000359275; ENSG00000137942 [Q5T0N5-3]
GeneIDi54874
KEGGihsa:54874
UCSCiuc001dpv.4 human [Q5T0N5-1]

Organism-specific databases

CTDi54874
DisGeNETi54874
EuPathDBiHostDB:ENSG00000137942.16
GeneCardsiFNBP1L
HGNCiHGNC:20851 FNBP1L
HPAiHPA065273
MIMi608848 gene
neXtProtiNX_Q5T0N5
OpenTargetsiENSG00000137942
PharmGKBiPA128394675
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410INNW Eukaryota
ENOG410XS31 LUCA
GeneTreeiENSGT00510000046403
HOGENOMiHOG000231767
HOVERGENiHBG002489
InParanoidiQ5T0N5
KOiK20121
PhylomeDBiQ5T0N5
TreeFamiTF351162

Enzyme and pathway databases

ReactomeiR-HSA-8856828 Clathrin-mediated endocytosis

Miscellaneous databases

ChiTaRSiFNBP1L human
GeneWikiiFNBP1L
GenomeRNAii54874
PROiPR:Q5T0N5
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000137942 Expressed in 228 organ(s), highest expression level in forebrain
CleanExiHS_FNBP1L
ExpressionAtlasiQ5T0N5 baseline and differential
GenevisibleiQ5T0N5 HS

Family and domain databases

CDDicd07675 F-BAR_FNBP1L, 1 hit
cd12072 SH3_FNBP1L, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR030116 FNBP1L
IPR035494 FNBP1L_F-BAR
IPR035493 FNBP1L_SH3
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR43990:SF3 PTHR43990:SF3, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF00018 SH3_1, 1 hit
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS51860 REM_1, 1 hit
PS50002 SH3, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiFBP1L_HUMAN
AccessioniPrimary (citable) accession number: Q5T0N5
Secondary accession number(s): J3QSS4
, Q5T0N6, Q6B097, Q6P653, Q6R4Q4, Q9NXG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: July 9, 2014
Last modified: October 10, 2018
This is version 138 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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