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Protein

Polynucleotide 5'-hydroxyl-kinase NOL9

Gene

NOL9

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Polynucleotide 5'-kinase involved in rRNA processing. The kinase activity is required for the processing of the 32S precursor into 5.8S and 28S rRNAs, more specifically for the generation of the major 5.8S(S) form. In vitro, has both DNA and RNA 5'-kinase activities. Probably binds RNA.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi306 – 313ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB
  • polynucleotide 5'-hydroxyl-kinase activity Source: UniProtKB
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) Source: GO_Central
  • maturation of 5.8S rRNA Source: UniProtKB
  • rRNA processing Source: Reactome

Keywordsi

Molecular functionKinase, RNA-binding, Transferase
Biological processrRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Polynucleotide 5'-hydroxyl-kinase NOL9 (EC:2.7.1.-)
Alternative name(s):
Nucleolar protein 9
Gene namesi
Name:NOL9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000162408.10
HGNCiHGNC:26265 NOL9
neXtProtiNX_Q5SY16

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi312K → A: Abolishes kinase activity and rRNA processing. 1 Publication1
Mutagenesisi313S → A: Abolishes kinase activity and rRNA processing. 1 Publication1

Organism-specific databases

DisGeNETi79707
OpenTargetsiENSG00000162408
PharmGKBiPA142671256

Polymorphism and mutation databases

BioMutaiNOL9
DMDMi73921242

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000969392 – 702Polynucleotide 5'-hydroxyl-kinase NOL9Add BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Cross-linki485Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei487PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5SY16
MaxQBiQ5SY16
PaxDbiQ5SY16
PeptideAtlasiQ5SY16
PRIDEiQ5SY16
ProteomicsDBi64010

2D gel databases

SWISS-2DPAGEiQ5SY16

PTM databases

iPTMnetiQ5SY16
PhosphoSitePlusiQ5SY16
SwissPalmiQ5SY16

Expressioni

Gene expression databases

BgeeiENSG00000162408 Expressed in 237 organ(s), highest expression level in forebrain
CleanExiHS_NOL9
GenevisibleiQ5SY16 HS

Organism-specific databases

HPAiHPA036347

Interactioni

Subunit structurei

Interacts with PELP1, WDR18 and SENP3.By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi122825, 44 interactors
CORUMiQ5SY16
IntActiQ5SY16, 24 interactors
MINTiQ5SY16
STRINGi9606.ENSP00000366934

Structurei

3D structure databases

ProteinModelPortaliQ5SY16
SMRiQ5SY16
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Clp1 family. NOL9/GRC3 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2750 Eukaryota
COG1341 LUCA
GeneTreeiENSGT00390000012406
HOGENOMiHOG000113821
HOVERGENiHBG058105
KOiK06947
OMAiMDGLYTK
OrthoDBiEOG091G0BNE
PhylomeDBiQ5SY16
TreeFamiTF313802

Family and domain databases

InterProiView protein in InterPro
IPR032319 CLP1_P
PfamiView protein in Pfam
PF16575 CLP1_P, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SY16-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADSGLLLKR GSCRSTWLRV RKARPQLILS RRPRRRLGSL RWCGRRRLRW
60 70 80 90 100
RLLQAQASGV DWREGARQVS RAAAARRPNT ATPSPIPSPT PASEPESEPE
110 120 130 140 150
LESASSCHRP LLIPPVRPVG PGRALLLLPV EQGFTFSGIC RVTCLYGQVQ
160 170 180 190 200
VFGFTISQGQ PAQDIFSVYT HSCLSIHALH YSQPEKSKKE LKREARNLLK
210 220 230 240 250
SHLNLDDRRW SMQNFSPQCS IVLLEHLKTA TVNFITSYPG SSYIFVQESP
260 270 280 290 300
TPQIKPEYLA LRSVGIRREK KRKGLQLTES TLSALEELVN VSCEEVDGCP
310 320 330 340 350
VILVCGSQDV GKSTFNRYLI NHLLNSLPCV DYLECDLGQT EFTPPGCISL
360 370 380 390 400
LNITEPVLGP PFTHLRTPQK MVYYGKPSCK NNYENYIDIV KYVFSAYKRE
410 420 430 440 450
SPLIVNTMGW VSDQGLLLLI DLIRLLSPSH VVQFRSDHSK YMPDLTPQYV
460 470 480 490 500
DDMDGLYTKS KTKMRNRRFR LAAFADALEF ADEEKESPVE FTGHKLIGVY
510 520 530 540 550
TDFAFRITPR NRESHNKILR DLSILSYLSQ LQPPMPKPLS PLHSLTPYQV
560 570 580 590 600
PFNAVALRIT HSDVAPTHIL YAVNASWVGL CKIQDDVRGY TNGPILLAQT
610 620 630 640 650
PICDCLGFGI CRGIDMEKRL YHILTPVPPE ELRTVNCLLV GAIAIPHCVL
660 670 680 690 700
KCQRGIEGTV PYVTTDYNFK LPGASEKIGA REPEEAHKEK PYRRPKFCRK

MK
Length:702
Mass (Da):79,323
Last modified:December 21, 2004 - v1
Checksum:i5D42AF5F7F292997
GO

Sequence cautioni

The sequence BAB15611 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05477710R → W2 PublicationsCorresponds to variant dbSNP:rs4908923Ensembl.1
Natural variantiVAR_05477850W → R2 PublicationsCorresponds to variant dbSNP:rs6693400Ensembl.1
Natural variantiVAR_05477958S → A2 PublicationsCorresponds to variant dbSNP:rs6693391Ensembl.1
Natural variantiVAR_056955420I → V. Corresponds to variant dbSNP:rs17029613Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026976 mRNA Translation: BAB15611.1 Different initiation.
AK289653 mRNA Translation: BAF82342.1
AL591866 Genomic DNA No translation available.
BC009257 mRNA Translation: AAH09257.2
BC012439 mRNA Translation: AAH12439.1
BC063639 mRNA Translation: AAH63639.1
BC094836 mRNA Translation: AAH94836.1
BC105095 mRNA Translation: AAI05096.1
BC110849 mRNA Translation: AAI10850.1
BC112278 mRNA Translation: AAI12279.1
CCDSiCCDS80.1
RefSeqiNP_078930.3, NM_024654.4
XP_011540449.1, XM_011542147.2
XP_016857827.1, XM_017002338.1
XP_016857828.1, XM_017002339.1
UniGeneiHs.59425

Genome annotation databases

EnsembliENST00000377705; ENSP00000366934; ENSG00000162408
GeneIDi79707
KEGGihsa:79707
UCSCiuc001ans.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK026976 mRNA Translation: BAB15611.1 Different initiation.
AK289653 mRNA Translation: BAF82342.1
AL591866 Genomic DNA No translation available.
BC009257 mRNA Translation: AAH09257.2
BC012439 mRNA Translation: AAH12439.1
BC063639 mRNA Translation: AAH63639.1
BC094836 mRNA Translation: AAH94836.1
BC105095 mRNA Translation: AAI05096.1
BC110849 mRNA Translation: AAI10850.1
BC112278 mRNA Translation: AAI12279.1
CCDSiCCDS80.1
RefSeqiNP_078930.3, NM_024654.4
XP_011540449.1, XM_011542147.2
XP_016857827.1, XM_017002338.1
XP_016857828.1, XM_017002339.1
UniGeneiHs.59425

3D structure databases

ProteinModelPortaliQ5SY16
SMRiQ5SY16
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122825, 44 interactors
CORUMiQ5SY16
IntActiQ5SY16, 24 interactors
MINTiQ5SY16
STRINGi9606.ENSP00000366934

PTM databases

iPTMnetiQ5SY16
PhosphoSitePlusiQ5SY16
SwissPalmiQ5SY16

Polymorphism and mutation databases

BioMutaiNOL9
DMDMi73921242

2D gel databases

SWISS-2DPAGEiQ5SY16

Proteomic databases

EPDiQ5SY16
MaxQBiQ5SY16
PaxDbiQ5SY16
PeptideAtlasiQ5SY16
PRIDEiQ5SY16
ProteomicsDBi64010

Protocols and materials databases

DNASUi79707
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377705; ENSP00000366934; ENSG00000162408
GeneIDi79707
KEGGihsa:79707
UCSCiuc001ans.4 human

Organism-specific databases

CTDi79707
DisGeNETi79707
EuPathDBiHostDB:ENSG00000162408.10
GeneCardsiNOL9
HGNCiHGNC:26265 NOL9
HPAiHPA036347
neXtProtiNX_Q5SY16
OpenTargetsiENSG00000162408
PharmGKBiPA142671256
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2750 Eukaryota
COG1341 LUCA
GeneTreeiENSGT00390000012406
HOGENOMiHOG000113821
HOVERGENiHBG058105
KOiK06947
OMAiMDGLYTK
OrthoDBiEOG091G0BNE
PhylomeDBiQ5SY16
TreeFamiTF313802

Enzyme and pathway databases

ReactomeiR-HSA-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

ChiTaRSiNOL9 human
GeneWikiiNOL9
GenomeRNAii79707
PROiPR:Q5SY16

Gene expression databases

BgeeiENSG00000162408 Expressed in 237 organ(s), highest expression level in forebrain
CleanExiHS_NOL9
GenevisibleiQ5SY16 HS

Family and domain databases

InterProiView protein in InterPro
IPR032319 CLP1_P
PfamiView protein in Pfam
PF16575 CLP1_P, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNOL9_HUMAN
AccessioniPrimary (citable) accession number: Q5SY16
Secondary accession number(s): Q2NL84
, Q4VBY3, Q6P472, Q7L4D6, Q96EE0, Q9H5L4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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