UniProtKB - Q5SX39 (MYH4_MOUSE)
Protein
Myosin-4
Gene
Myh4
Organism
Mus musculus (Mouse)
Status
Functioni
Muscle contraction.
Caution
Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).Curated
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 179 – 186 | ATPSequence analysis | 8 |
GO - Molecular functioni
- actin filament binding Source: InterPro
- ATP binding Source: UniProtKB-KW
- calmodulin binding Source: UniProtKB-KW
- double-stranded RNA binding Source: MGI
- motor activity Source: InterPro
GO - Biological processi
- muscle contraction Source: MGI
- response to activity Source: MGI
Keywordsi
Molecular function | Actin-binding, Calmodulin-binding, Motor protein, Muscle protein, Myosin |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Myosin-4Alternative name(s): Myosin heavy chain 2b Short name: MyHC-2b Myosin heavy chain 4 |
Gene namesi | Name:Myh4 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1339713, Myh4 |
Subcellular locationi
Other locations
Note: Thick filaments of the myofibrils.
Cytoskeleton
- myosin complex Source: UniProtKB-KW
Other locations
- myofibril Source: MGI
- myosin filament Source: UniProtKB-KW
Keywords - Cellular componenti
Cytoplasm, Thick filamentPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000123399 | 1 – 1939 | Myosin-4Add BLAST | 1939 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 36 | PhosphoserineBy similarity | 1 | |
Modified residuei | 64 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 69 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 79 | PhosphoserineBy similarity | 1 | |
Modified residuei | 130 | N6,N6,N6-trimethyllysineSequence analysis | 1 | |
Modified residuei | 389 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 391 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 392 | PhosphoserineBy similarity | 1 | |
Modified residuei | 419 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 424 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 625 | PhosphoserineBy similarity | 1 | |
Modified residuei | 757 | Pros-methylhistidineBy similarity | 1 | |
Modified residuei | 776 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1092 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1096 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1162 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1237 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1241 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1243 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1255 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1261 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1265 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1278 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1286 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1288 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1292 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1303 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1306 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1413 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1464 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 1467 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1474 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1492 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 1495 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1501 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1514 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1517 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1542 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1547 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1554 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1574 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1600 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1603 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1714 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1726 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1730 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1736 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1739 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q5SX39 |
MaxQBi | Q5SX39 |
PaxDbi | Q5SX39 |
PeptideAtlasi | Q5SX39 |
PRIDEi | Q5SX39 |
2D gel databases
UCD-2DPAGEi | Q5SX39 |
PTM databases
iPTMneti | Q5SX39 |
PhosphoSitePlusi | Q5SX39 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000057003, Expressed in muscle of leg and 135 other tissues |
Genevisiblei | Q5SX39, MM |
Interactioni
Subunit structurei
Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
GO - Molecular functioni
- actin filament binding Source: InterPro
- calmodulin binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 201648, 7 interactors |
STRINGi | 10090.ENSMUSP00000018632 |
Miscellaneous databases
RNActi | Q5SX39, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 33 – 82 | Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST | 50 | |
Domaini | 86 – 782 | Myosin motorPROSITE-ProRule annotationAdd BLAST | 697 | |
Domaini | 785 – 814 | IQPROSITE-ProRule annotationAdd BLAST | 30 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 659 – 681 | Actin-bindingBy similarityAdd BLAST | 23 | |
Regioni | 761 – 775 | Actin-bindingBy similarityAdd BLAST | 15 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 843 – 1939 | Sequence analysisAdd BLAST | 1097 |
Domaini
The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated
Sequence similaritiesi
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0161, Eukaryota |
GeneTreei | ENSGT00940000163646 |
HOGENOMi | CLU_000192_8_1_1 |
InParanoidi | Q5SX39 |
OMAi | TQGILKX |
OrthoDBi | 47111at2759 |
PhylomeDBi | Q5SX39 |
TreeFami | TF314375 |
Family and domain databases
Gene3Di | 2.30.30.360, 1 hit 3.40.850.10, 1 hit 4.10.270.10, 1 hit |
InterProi | View protein in InterPro IPR000048, IQ_motif_EF-hand-BS IPR036961, Kinesin_motor_dom_sf IPR001609, Myosin_head_motor_dom IPR027401, Myosin_IQ_contain_sf IPR004009, Myosin_N IPR008989, Myosin_S1_N IPR002928, Myosin_tail IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00063, Myosin_head, 1 hit PF02736, Myosin_N, 1 hit PF01576, Myosin_tail_1, 1 hit |
PRINTSi | PR00193, MYOSINHEAVY |
SMARTi | View protein in SMART SM00242, MYSc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50096, IQ, 1 hit PS51456, MYOSIN_MOTOR, 1 hit PS51844, SH3_LIKE, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5SX39-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSDAEMAVF GEAAPYLRKS EKERIEAQNK PFDAKSSVFV VDAKESYVKA
60 70 80 90 100
TVQSREGGKV TAKTEGGATV TVKDDQVFSM NPPKYDKIED MAMMTHLHEP
110 120 130 140 150
AVLYNLKERY AAWMIYTYSG LFCVTVNPYK WLPVYNPEVV AAYRGKKRQE
160 170 180 190 200
APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA
210 220 230 240 250
VTGDKKKEEA TSGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF
260 270 280 290 300
IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QIMSNKKPEL
310 320 330 340 350
IEMLLITTNP YDFAYVSQGE ITVPSIDDQE ELMATDTAVD ILGFSADEKV
360 370 380 390 400
AIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK
410 420 430 440 450
ALCYPRVKVG NEYVTKGQTV QQVYNSVGAL AKSMYEKMFL WMVTRINQQL
460 470 480 490 500
DTKQPRQYFI GVLDIAGFEI FDFNTLEQLC INFTNEKLQQ FFNHHMFVLE
510 520 530 540 550
QEEYKKEGID WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS
560 570 580 590 600
FKNKLYEQHL GKSNNFQKPK PAKGKAEAHF SLVHYAGTVD YNIIGWLDKN
610 620 630 640 650
KDPLNETVVG LYQKSGLKTL AFLFSGGQAA EAEGGGGKKG GKKKGSSFQT
660 670 680 690 700
VSALFRENLN KLMTNLKSTH PHFVRCLIPN ETKTPGAMEH ELVLHQLRCN
710 720 730 740 750
GVLEGIRICR KGFPSRILYA DFKQRYKVLN ASAIPEGQFI DSKKASEKLL
760 770 780 790 800
GSIDIDHTQY KFGHTKVFFK AGLLGTLEEM RDEKLAQLIT RTQAVCRGYL
810 820 830 840 850
MRVEFKKMME RRESIFCIQY NVRAFMNVKH WPWMKLYFKI KPLLKSAETE
860 870 880 890 900
KEMANMKEDF EKAKEDLAKS EAKRKELEEK MVALMQEKND LQLQVQAEAD
910 920 930 940 950
GLADAEERCD QLIKTKIQLE AKIKELTERA EDEEEINAEL TAKKRKLEDE
960 970 980 990 1000
CSELKKDIDD LELTLAKVEK EKHATENKVK NLTEEMAGLD ENIAKLTKEK
1010 1020 1030 1040 1050
KALQEAHQQT LDDLQAEEDK VNTLTKAKTK LEQQVDDLEG SLEQEKKLRM
1060 1070 1080 1090 1100
DLERAKRKLE GDLKLAQEST MDIENDKQQL DEKLKKKEFE MSNLQSKIED
1110 1120 1130 1140 1150
EQALGMQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR SDLSRELEEI
1160 1170 1180 1190 1200
SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEEATLQHE ATAAALRKKH
1210 1220 1230 1240 1250
ADSVAELGEQ IDNLQRVKQK LEKEKSELKM EIDDLASNME TVSKAKGNLE
1260 1270 1280 1290 1300
KMCRTLEDQL SEVKTKEEEQ QRLINELSTQ KARLHTESGE FSRQLDEKDA
1310 1320 1330 1340 1350
MVSQLSRGKQ AFTQQIEELK RQLEEESKAK NALAHALQSA RHDCDLLREQ
1360 1370 1380 1390 1400
YEEEQEAKAE LQRAMSKANS EVAQWRTKYE TDAIQRTEEL EEAKKKLAQR
1410 1420 1430 1440 1450
LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN AACAALDKKQ
1460 1470 1480 1490 1500
RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDQLE
1510 1520 1530 1540 1550
TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKIKKQIDQE KSELQASLEE
1560 1570 1580 1590 1600
AEASLEHEEG KILRIQLELN QVKSEIDRKI AEKDEEIDQL KRNHLRVVES
1610 1620 1630 1640 1650
MQSTLDAEIR SRNDALRIKK KMEGDLNEME IQLNHANRQA AEAIRNLRNT
1660 1670 1680 1690 1700
QGMLKDTQLH LDDALRGQDD LKEQLAMVER RANLMQAEIE ELRASLEQTE
1710 1720 1730 1740 1750
RSRRVAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ IQGEMEDIVQ
1760 1770 1780 1790 1800
EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQHR
1810 1820 1830 1840 1850
LDEAEQLALK GGKKQIQKLE ARVRELENEV ENEQKRNIEA VKGLRKHERR
1860 1870 1880 1890 1900
VKELTYQTEE DRKNVLRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF
1910 1920 1930
RKIQHELEEA EERADIAESQ VNKLRVKSRE VHTKVISEE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL596129 Genomic DNA No translation available. BC141362 mRNA Translation: AAI41363.1 |
CCDSi | CCDS24856.1 |
RefSeqi | NP_034985.2, NM_010855.3 |
Genome annotation databases
Ensembli | ENSMUST00000018632; ENSMUSP00000018632; ENSMUSG00000057003 ENSMUST00000170942; ENSMUSP00000127514; ENSMUSG00000057003 |
GeneIDi | 17884 |
KEGGi | mmu:17884 |
UCSCi | uc007jmj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL596129 Genomic DNA No translation available. BC141362 mRNA Translation: AAI41363.1 |
CCDSi | CCDS24856.1 |
RefSeqi | NP_034985.2, NM_010855.3 |
3D structure databases
SMRi | Q5SX39 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 201648, 7 interactors |
STRINGi | 10090.ENSMUSP00000018632 |
PTM databases
iPTMneti | Q5SX39 |
PhosphoSitePlusi | Q5SX39 |
2D gel databases
UCD-2DPAGEi | Q5SX39 |
Proteomic databases
EPDi | Q5SX39 |
MaxQBi | Q5SX39 |
PaxDbi | Q5SX39 |
PeptideAtlasi | Q5SX39 |
PRIDEi | Q5SX39 |
Genome annotation databases
Ensembli | ENSMUST00000018632; ENSMUSP00000018632; ENSMUSG00000057003 ENSMUST00000170942; ENSMUSP00000127514; ENSMUSG00000057003 |
GeneIDi | 17884 |
KEGGi | mmu:17884 |
UCSCi | uc007jmj.2, mouse |
Organism-specific databases
CTDi | 4622 |
MGIi | MGI:1339713, Myh4 |
Phylogenomic databases
eggNOGi | KOG0161, Eukaryota |
GeneTreei | ENSGT00940000163646 |
HOGENOMi | CLU_000192_8_1_1 |
InParanoidi | Q5SX39 |
OMAi | TQGILKX |
OrthoDBi | 47111at2759 |
PhylomeDBi | Q5SX39 |
TreeFami | TF314375 |
Miscellaneous databases
BioGRID-ORCSi | 17884, 2 hits in 18 CRISPR screens |
PROi | PR:Q5SX39 |
RNActi | Q5SX39, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000057003, Expressed in muscle of leg and 135 other tissues |
Genevisiblei | Q5SX39, MM |
Family and domain databases
Gene3Di | 2.30.30.360, 1 hit 3.40.850.10, 1 hit 4.10.270.10, 1 hit |
InterProi | View protein in InterPro IPR000048, IQ_motif_EF-hand-BS IPR036961, Kinesin_motor_dom_sf IPR001609, Myosin_head_motor_dom IPR027401, Myosin_IQ_contain_sf IPR004009, Myosin_N IPR008989, Myosin_S1_N IPR002928, Myosin_tail IPR027417, P-loop_NTPase |
Pfami | View protein in Pfam PF00063, Myosin_head, 1 hit PF02736, Myosin_N, 1 hit PF01576, Myosin_tail_1, 1 hit |
PRINTSi | PR00193, MYOSINHEAVY |
SMARTi | View protein in SMART SM00242, MYSc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS50096, IQ, 1 hit PS51456, MYOSIN_MOTOR, 1 hit PS51844, SH3_LIKE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MYH4_MOUSE | |
Accessioni | Q5SX39Primary (citable) accession number: Q5SX39 Secondary accession number(s): B9EJ73 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2005 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 10, 2021 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families