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Protein

Myosin-4

Gene

Myh4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Muscle contraction.

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).Curated

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi179 – 186ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • muscle contraction Source: MGI
  • response to activity Source: MGI

Keywordsi

Molecular functionActin-binding, Calmodulin-binding, Motor protein, Muscle protein, Myosin
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-4
Alternative name(s):
Myosin heavy chain 2b
Short name:
MyHC-2b
Myosin heavy chain 4
Gene namesi
Name:Myh4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1339713 Myh4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001233991 – 1939Myosin-4Add BLAST1939

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei36PhosphoserineBy similarity1
Modified residuei64PhosphothreonineBy similarity1
Modified residuei69PhosphothreonineBy similarity1
Modified residuei79PhosphoserineBy similarity1
Modified residuei130N6,N6,N6-trimethyllysineSequence analysis1
Modified residuei389PhosphotyrosineBy similarity1
Modified residuei391PhosphothreonineBy similarity1
Modified residuei392PhosphoserineBy similarity1
Modified residuei419PhosphothreonineBy similarity1
Modified residuei424PhosphotyrosineBy similarity1
Modified residuei625PhosphoserineBy similarity1
Modified residuei757Pros-methylhistidineBy similarity1
Modified residuei776PhosphothreonineBy similarity1
Modified residuei1092PhosphoserineBy similarity1
Modified residuei1096PhosphoserineBy similarity1
Modified residuei1162PhosphoserineBy similarity1
Modified residuei1237PhosphoserineBy similarity1
Modified residuei1241PhosphothreonineBy similarity1
Modified residuei1243PhosphoserineBy similarity1
Modified residuei1255PhosphothreonineBy similarity1
Modified residuei1261PhosphoserineBy similarity1
Modified residuei1265PhosphothreonineBy similarity1
Modified residuei1278PhosphoserineBy similarity1
Modified residuei1286PhosphothreonineBy similarity1
Modified residuei1288PhosphoserineBy similarity1
Modified residuei1292PhosphoserineBy similarity1
Modified residuei1303PhosphoserineBy similarity1
Modified residuei1306PhosphoserineBy similarity1
Modified residuei1413PhosphoserineBy similarity1
Modified residuei1464PhosphotyrosineBy similarity1
Modified residuei1467PhosphothreonineBy similarity1
Modified residuei1474PhosphoserineBy similarity1
Modified residuei1492PhosphotyrosineBy similarity1
Modified residuei1495PhosphoserineBy similarity1
Modified residuei1501PhosphothreonineBy similarity1
Modified residuei1514PhosphoserineBy similarity1
Modified residuei1517PhosphothreonineBy similarity1
Modified residuei1542PhosphoserineBy similarity1
Modified residuei1547PhosphoserineBy similarity1
Modified residuei1554PhosphoserineBy similarity1
Modified residuei1574PhosphoserineBy similarity1
Modified residuei1600PhosphoserineBy similarity1
Modified residuei1603PhosphoserineBy similarity1
Modified residuei1714PhosphoserineBy similarity1
Modified residuei1726PhosphoserineBy similarity1
Modified residuei1730PhosphothreonineBy similarity1
Modified residuei1736PhosphothreonineBy similarity1
Modified residuei1739PhosphoserineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiQ5SX39
MaxQBiQ5SX39
PaxDbiQ5SX39
PeptideAtlasiQ5SX39
PRIDEiQ5SX39

2D gel databases

UCD-2DPAGEiQ5SX39

PTM databases

iPTMnetiQ5SX39
PhosphoSitePlusiQ5SX39

Expressioni

Gene expression databases

BgeeiENSMUSG00000057003 Expressed in 120 organ(s), highest expression level in muscle of leg
CleanExiMM_MYH4
GenevisibleiQ5SX39 MM

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201648, 3 interactors
STRINGi10090.ENSMUSP00000018632

Structurei

3D structure databases

ProteinModelPortaliQ5SX39
SMRiQ5SX39
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 82Myosin N-terminal SH3-likePROSITE-ProRule annotationAdd BLAST50
Domaini86 – 782Myosin motorPROSITE-ProRule annotationAdd BLAST697
Domaini785 – 814IQPROSITE-ProRule annotationAdd BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni659 – 681Actin-bindingBy similarityAdd BLAST23
Regioni761 – 775Actin-bindingBy similarityAdd BLAST15

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili843 – 1939Sequence analysisAdd BLAST1097

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161 Eukaryota
COG5022 LUCA
GeneTreeiENSGT00930000150860
HOGENOMiHOG000173959
HOVERGENiHBG004704
InParanoidiQ5SX39
KOiK10352
OMAiHANKANE
OrthoDBiEOG091G07UM
PhylomeDBiQ5SX39
TreeFamiTF314375

Family and domain databases

Gene3Di2.30.30.360, 1 hit
3.40.850.10, 1 hit
4.10.270.10, 1 hit
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR027401 Myosin_IQ_contain_sf
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR002928 Myosin_tail
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00063 Myosin_head, 1 hit
PF02736 Myosin_N, 1 hit
PF01576 Myosin_tail_1, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 1 hit
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Q5SX39-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSSDAEMAVF GEAAPYLRKS EKERIEAQNK PFDAKSSVFV VDAKESYVKA
60 70 80 90 100
TVQSREGGKV TAKTEGGATV TVKDDQVFSM NPPKYDKIED MAMMTHLHEP
110 120 130 140 150
AVLYNLKERY AAWMIYTYSG LFCVTVNPYK WLPVYNPEVV AAYRGKKRQE
160 170 180 190 200
APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA
210 220 230 240 250
VTGDKKKEEA TSGKMQGTLE DQIISANPLL EAFGNAKTVR NDNSSRFGKF
260 270 280 290 300
IRIHFGATGK LASADIETYL LEKSRVTFQL KAERSYHIFY QIMSNKKPEL
310 320 330 340 350
IEMLLITTNP YDFAYVSQGE ITVPSIDDQE ELMATDTAVD ILGFSADEKV
360 370 380 390 400
AIYKLTGAVM HYGNMKFKQK QREEQAEPDG TEVADKAAYL TSLNSADLLK
410 420 430 440 450
ALCYPRVKVG NEYVTKGQTV QQVYNSVGAL AKSMYEKMFL WMVTRINQQL
460 470 480 490 500
DTKQPRQYFI GVLDIAGFEI FDFNTLEQLC INFTNEKLQQ FFNHHMFVLE
510 520 530 540 550
QEEYKKEGID WEFIDFGMDL AACIELIEKP MGIFSILEEE CMFPKATDTS
560 570 580 590 600
FKNKLYEQHL GKSNNFQKPK PAKGKAEAHF SLVHYAGTVD YNIIGWLDKN
610 620 630 640 650
KDPLNETVVG LYQKSGLKTL AFLFSGGQAA EAEGGGGKKG GKKKGSSFQT
660 670 680 690 700
VSALFRENLN KLMTNLKSTH PHFVRCLIPN ETKTPGAMEH ELVLHQLRCN
710 720 730 740 750
GVLEGIRICR KGFPSRILYA DFKQRYKVLN ASAIPEGQFI DSKKASEKLL
760 770 780 790 800
GSIDIDHTQY KFGHTKVFFK AGLLGTLEEM RDEKLAQLIT RTQAVCRGYL
810 820 830 840 850
MRVEFKKMME RRESIFCIQY NVRAFMNVKH WPWMKLYFKI KPLLKSAETE
860 870 880 890 900
KEMANMKEDF EKAKEDLAKS EAKRKELEEK MVALMQEKND LQLQVQAEAD
910 920 930 940 950
GLADAEERCD QLIKTKIQLE AKIKELTERA EDEEEINAEL TAKKRKLEDE
960 970 980 990 1000
CSELKKDIDD LELTLAKVEK EKHATENKVK NLTEEMAGLD ENIAKLTKEK
1010 1020 1030 1040 1050
KALQEAHQQT LDDLQAEEDK VNTLTKAKTK LEQQVDDLEG SLEQEKKLRM
1060 1070 1080 1090 1100
DLERAKRKLE GDLKLAQEST MDIENDKQQL DEKLKKKEFE MSNLQSKIED
1110 1120 1130 1140 1150
EQALGMQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR SDLSRELEEI
1160 1170 1180 1190 1200
SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEEATLQHE ATAAALRKKH
1210 1220 1230 1240 1250
ADSVAELGEQ IDNLQRVKQK LEKEKSELKM EIDDLASNME TVSKAKGNLE
1260 1270 1280 1290 1300
KMCRTLEDQL SEVKTKEEEQ QRLINELSTQ KARLHTESGE FSRQLDEKDA
1310 1320 1330 1340 1350
MVSQLSRGKQ AFTQQIEELK RQLEEESKAK NALAHALQSA RHDCDLLREQ
1360 1370 1380 1390 1400
YEEEQEAKAE LQRAMSKANS EVAQWRTKYE TDAIQRTEEL EEAKKKLAQR
1410 1420 1430 1440 1450
LQDAEEHVEA VNSKCASLEK TKQRLQNEVE DLMIDVERSN AACAALDKKQ
1460 1470 1480 1490 1500
RNFDKVLAEW KQKYEETQAE LEASQKESRS LSTELFKVKN AYEESLDQLE
1510 1520 1530 1540 1550
TLKRENKNLQ QEISDLTEQI AEGGKHIHEL EKIKKQIDQE KSELQASLEE
1560 1570 1580 1590 1600
AEASLEHEEG KILRIQLELN QVKSEIDRKI AEKDEEIDQL KRNHLRVVES
1610 1620 1630 1640 1650
MQSTLDAEIR SRNDALRIKK KMEGDLNEME IQLNHANRQA AEAIRNLRNT
1660 1670 1680 1690 1700
QGMLKDTQLH LDDALRGQDD LKEQLAMVER RANLMQAEIE ELRASLEQTE
1710 1720 1730 1740 1750
RSRRVAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ IQGEMEDIVQ
1760 1770 1780 1790 1800
EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNM EQTVKDLQHR
1810 1820 1830 1840 1850
LDEAEQLALK GGKKQIQKLE ARVRELENEV ENEQKRNIEA VKGLRKHERR
1860 1870 1880 1890 1900
VKELTYQTEE DRKNVLRLQD LVDKLQTKVK AYKRQAEEAE EQSNVNLAKF
1910 1920 1930
RKIQHELEEA EERADIAESQ VNKLRVKSRE VHTKVISEE
Length:1,939
Mass (Da):222,859
Last modified:December 21, 2004 - v1
Checksum:iF4334DF4D674A244
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL596129 Genomic DNA No translation available.
BC141362 mRNA Translation: AAI41363.1
CCDSiCCDS24856.1
RefSeqiNP_034985.2, NM_010855.3
UniGeneiMm.297382

Genome annotation databases

EnsembliENSMUST00000018632; ENSMUSP00000018632; ENSMUSG00000057003
ENSMUST00000170942; ENSMUSP00000127514; ENSMUSG00000057003
GeneIDi17884
KEGGimmu:17884
UCSCiuc007jmj.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL596129 Genomic DNA No translation available.
BC141362 mRNA Translation: AAI41363.1
CCDSiCCDS24856.1
RefSeqiNP_034985.2, NM_010855.3
UniGeneiMm.297382

3D structure databases

ProteinModelPortaliQ5SX39
SMRiQ5SX39
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201648, 3 interactors
STRINGi10090.ENSMUSP00000018632

PTM databases

iPTMnetiQ5SX39
PhosphoSitePlusiQ5SX39

2D gel databases

UCD-2DPAGEiQ5SX39

Proteomic databases

EPDiQ5SX39
MaxQBiQ5SX39
PaxDbiQ5SX39
PeptideAtlasiQ5SX39
PRIDEiQ5SX39

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018632; ENSMUSP00000018632; ENSMUSG00000057003
ENSMUST00000170942; ENSMUSP00000127514; ENSMUSG00000057003
GeneIDi17884
KEGGimmu:17884
UCSCiuc007jmj.2 mouse

Organism-specific databases

CTDi4622
MGIiMGI:1339713 Myh4

Phylogenomic databases

eggNOGiKOG0161 Eukaryota
COG5022 LUCA
GeneTreeiENSGT00930000150860
HOGENOMiHOG000173959
HOVERGENiHBG004704
InParanoidiQ5SX39
KOiK10352
OMAiHANKANE
OrthoDBiEOG091G07UM
PhylomeDBiQ5SX39
TreeFamiTF314375

Miscellaneous databases

PROiPR:Q5SX39
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057003 Expressed in 120 organ(s), highest expression level in muscle of leg
CleanExiMM_MYH4
GenevisibleiQ5SX39 MM

Family and domain databases

Gene3Di2.30.30.360, 1 hit
3.40.850.10, 1 hit
4.10.270.10, 1 hit
InterProiView protein in InterPro
IPR000048 IQ_motif_EF-hand-BS
IPR036961 Kinesin_motor_dom_sf
IPR001609 Myosin_head_motor_dom
IPR027401 Myosin_IQ_contain_sf
IPR004009 Myosin_N
IPR008989 Myosin_S1_N
IPR002928 Myosin_tail
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00063 Myosin_head, 1 hit
PF02736 Myosin_N, 1 hit
PF01576 Myosin_tail_1, 1 hit
PRINTSiPR00193 MYOSINHEAVY
SMARTiView protein in SMART
SM00242 MYSc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS50096 IQ, 1 hit
PS51456 MYOSIN_MOTOR, 1 hit
PS51844 SH3_LIKE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMYH4_MOUSE
AccessioniPrimary (citable) accession number: Q5SX39
Secondary accession number(s): B9EJ73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 122 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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