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Protein

Rho GTPase-activating protein 44

Gene

Arhgap44

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

GTPase-activating protein (GAP) that stimulates the GTPase activity of Rho-type GTPases. Thereby, controls Rho-type GTPases cycling between their active GTP-bound and inactive GDP-bound states. May act as a GAP for CDC42 and RAC1. Endosomal recycling protein which, in association with SHANK3, is involved in synaptic plasticity. Promotes GRIA1 exocytosis from recycling endosomes and spine morphological changes associated to long-term potentiation.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: SynGO-UCL
  • phospholipid binding Source: MGI
  • Rac GTPase binding Source: SynGO-UCL

GO - Biological processi

Keywordsi

Molecular functionGTPase activation

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 44
Alternative name(s):
Rho-type GTPase-activating protein RICH2
RhoGAP interacting with CIP4 homologs protein 2
Short name:
RICH-2
Gene namesi
Name:Arhgap44
Synonyms:Rich2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2144423 Arhgap44

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Endosome, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi291R → A: Reduces GRIA1 recycling. Abolishes the increase of spine density and size, as well as reduces the increase of GRIA1 expression in surface associated with long-term potentiation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002804811 – 814Rho GTPase-activating protein 44Add BLAST814

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei493PhosphoserineCombined sources1
Modified residuei805PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5SSM3
PeptideAtlasiQ5SSM3
PRIDEiQ5SSM3

PTM databases

iPTMnetiQ5SSM3
PhosphoSitePlusiQ5SSM3
SwissPalmiQ5SSM3

Expressioni

Tissue specificityi

Expressed in hippocampal neurons (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000033389 Expressed in 234 organ(s), highest expression level in brain
CleanExiMM_AU040829
ExpressionAtlasiQ5SSM3 baseline and differential
GenevisibleiQ5SSM3 MM

Interactioni

Subunit structurei

Interacts with BST2 (via cytoplasmic domain).Interacts (probably via PDZ-binding motif) with SHANK3 (via PDZ domain); the interaction takes place in dendritic spines and promotes GRIA1 exocytosis.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229799, 2 interactors
IntActiQ5SSM3, 2 interactors
STRINGi10090.ENSMUSP00000039139

Structurei

3D structure databases

ProteinModelPortaliQ5SSM3
SMRiQ5SSM3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 249BARPROSITE-ProRule annotationAdd BLAST236
Domaini255 – 445Rho-GAPPROSITE-ProRule annotationAdd BLAST191

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni727 – 814Interaction with BST2By similarityAdd BLAST88

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi811 – 814PDZ-bindingCurated4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi540 – 740Pro-richAdd BLAST201

Domaini

Rho-GAP domain is required to promote GRIA1 exocytosis from recycling endosomes. Rho-GAP and BAR domains are necessary for the control of long-term potentiation in hippocampal neurons (PubMed:23739967).1 Publication

Phylogenomic databases

eggNOGiKOG4270 Eukaryota
ENOG410XRR2 LUCA
GeneTreeiENSGT00760000118863
HOVERGENiHBG000015
InParanoidiQ5SSM3
KOiK21067
OrthoDBiEOG091G02YP
PhylomeDBiQ5SSM3
TreeFamiTF316514

Family and domain databases

Gene3Di1.10.555.10, 1 hit
1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR004148 BAR_dom
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SM00324 RhoGAP, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
PS50238 RHOGAP, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5SSM3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKKQFNRMRQ LANQTVGRAE KTEVLSEDLL QVEKRLELVK QVSHSTHKKL
60 70 80 90 100
TACLQGQQGA EADKRSKKLP LTTLAQCLVE GSAILGDDTL LGKMLKLCGE
110 120 130 140 150
TEDKLAQELI HFELQVERDV IEPLFLLAEV EIPNIQKQRK HLAKLVLDMD
160 170 180 190 200
SSRTRWQQTS KSSGLSSSLQ PAGAKADALR EEMEEAANRV EICRDQLSAD
210 220 230 240 250
MYSFVAKEID YANYFQTLIE VQAEYHRKSL TLLQAVLPQI KAQQEAWVEK
260 270 280 290 300
PSFGKPLEEH LMISGREIAF PIEACVTMLL ECGMQEEGLF RVAPSASKLK
310 320 330 340 350
KLKAALDCCV VDVQEYSADP HAIAGALKSY LRELPEPLMT FELYDEWIQA
360 370 380 390 400
SNIQEQDKRL QALWNACEKL PKANHNNIKY LIKFLSKLSE YQDVNKMTPS
410 420 430 440 450
NMAIVLGPNL LWPQSEGNIT EMMTTVSLQI VGIIEPIIQH ADWFFPGEIE
460 470 480 490 500
FNLTGSYGSP VHVNHNANYS SMPSPDMDPA DRRQPEQARR PLSVATDNMM
510 520 530 540 550
LEFYKKDGLR KIQSMGVRVM DTSWVARRGS SAGRKASCAP PSMQPPAPPS
560 570 580 590 600
ELAAPLPSPL PEQVPDSPAT PAPALSPSGA SLQPTPERPS VSKSKELSPG
610 620 630 640 650
SGQKGSPGSI QGTPCPGTQL GPQPAASPSQ LPADQSPHTL RKVSKKVAPI
660 670 680 690 700
PPKVPFVQPG TVSDQPVGQP SPVSLSPTPP STPSPYGLSY PPGYSMASGQ
710 720 730 740 750
LSPASAPPLA SPSVFTSTLA KSRPTPKPRQ RPTLPPPQPP SVSLSASSPQ
760 770 780 790 800
STEHPMLDGM SPGESMSTDL VHFDVPSIHI ELGSTLRLSP LEHARRHSAT
810
DKRDSEEESE STAL
Length:814
Mass (Da):88,994
Last modified:December 21, 2004 - v1
Checksum:i466419A6D9471DDF
GO
Isoform 2 (identifier: Q5SSM3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     509-514: Missing.
     769-814: DLVHFDVPSIHIELGSTLRLSPLEHARRHSATDKRDSEEESESTAL → AV

Note: No experimental confirmation available. Contains a PDZ-binding motif at positions 761-764.
Show »
Length:764
Mass (Da):83,273
Checksum:iB0FF91EA208D8839
GO
Isoform 3 (identifier: Q5SSM3-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     769-814: DLVHFDVPSIHIELGSTLRLSPLEHARRHSATDKRDSEEESESTAL → AV

Note: Contains a PDZ-binding motif at positions 767-770.
Show »
Length:770
Mass (Da):83,999
Checksum:i45D4E8973896F0AB
GO
Isoform 4 (identifier: Q5SSM3-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-18: MKKQFNRMRQLANQTVGR → MELPGLELVRPSWVRAGRW
     195-210: DQLSADMYSFVAKEID → VPVPLALLSRDRLRSP
     211-814: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:211
Mass (Da):23,619
Checksum:i16E8F29D110BE5FD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6T1Y2F6T1Y2_MOUSE
Rho GTPase-activating protein 44
Arhgap44
645Annotation score:

Sequence cautioni

The sequence BAC35316 differs from that shown. Reason: Frameshift at position 19.Curated
Isoform 4 : The sequence BAC35316 differs from that shown. Reason: Frameshift at position 175.Curated
The sequence CAI24614 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0237371 – 18MKKQF…QTVGR → MELPGLELVRPSWVRAGRW in isoform 4. 1 PublicationAdd BLAST18
Alternative sequenceiVSP_023738195 – 210DQLSA…AKEID → VPVPLALLSRDRLRSP in isoform 4. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_023739211 – 814Missing in isoform 4. 1 PublicationAdd BLAST604
Alternative sequenceiVSP_023740509 – 514Missing in isoform 2. 1 Publication6
Alternative sequenceiVSP_023741769 – 814DLVHF…ESTAL → AV in isoform 2 and isoform 3. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053220 mRNA Translation: BAC35316.1 Frameshift.
AL663045 Genomic DNA Translation: CAI24613.2
AL663045 Genomic DNA Translation: CAI24614.1 Sequence problems.
AL663045 Genomic DNA Translation: CAI24616.1
AL663045 Genomic DNA Translation: CAI24617.1
BC056366 mRNA Translation: AAH56366.1
BC059911 mRNA Translation: AAH59911.1
CCDSiCCDS36180.1 [Q5SSM3-2]
RefSeqiNP_001092758.1, NM_001099288.1
NP_778168.2, NM_175003.3 [Q5SSM3-2]
XP_006532934.1, XM_006532871.3 [Q5SSM3-1]
XP_006532935.1, XM_006532872.3 [Q5SSM3-3]
UniGeneiMm.134338

Genome annotation databases

EnsembliENSMUST00000047463; ENSMUSP00000039139; ENSMUSG00000033389 [Q5SSM3-2]
ENSMUST00000093001; ENSMUSP00000090680; ENSMUSG00000033389 [Q5SSM3-4]
ENSMUST00000093002; ENSMUSP00000090681; ENSMUSG00000033389 [Q5SSM3-1]
GeneIDi216831
KEGGimmu:216831
UCSCiuc007jku.1 mouse [Q5SSM3-2]
uc007jkv.1 mouse [Q5SSM3-1]
uc007jkw.1 mouse [Q5SSM3-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK053220 mRNA Translation: BAC35316.1 Frameshift.
AL663045 Genomic DNA Translation: CAI24613.2
AL663045 Genomic DNA Translation: CAI24614.1 Sequence problems.
AL663045 Genomic DNA Translation: CAI24616.1
AL663045 Genomic DNA Translation: CAI24617.1
BC056366 mRNA Translation: AAH56366.1
BC059911 mRNA Translation: AAH59911.1
CCDSiCCDS36180.1 [Q5SSM3-2]
RefSeqiNP_001092758.1, NM_001099288.1
NP_778168.2, NM_175003.3 [Q5SSM3-2]
XP_006532934.1, XM_006532871.3 [Q5SSM3-1]
XP_006532935.1, XM_006532872.3 [Q5SSM3-3]
UniGeneiMm.134338

3D structure databases

ProteinModelPortaliQ5SSM3
SMRiQ5SSM3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi229799, 2 interactors
IntActiQ5SSM3, 2 interactors
STRINGi10090.ENSMUSP00000039139

PTM databases

iPTMnetiQ5SSM3
PhosphoSitePlusiQ5SSM3
SwissPalmiQ5SSM3

Proteomic databases

PaxDbiQ5SSM3
PeptideAtlasiQ5SSM3
PRIDEiQ5SSM3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047463; ENSMUSP00000039139; ENSMUSG00000033389 [Q5SSM3-2]
ENSMUST00000093001; ENSMUSP00000090680; ENSMUSG00000033389 [Q5SSM3-4]
ENSMUST00000093002; ENSMUSP00000090681; ENSMUSG00000033389 [Q5SSM3-1]
GeneIDi216831
KEGGimmu:216831
UCSCiuc007jku.1 mouse [Q5SSM3-2]
uc007jkv.1 mouse [Q5SSM3-1]
uc007jkw.1 mouse [Q5SSM3-4]

Organism-specific databases

CTDi9912
MGIiMGI:2144423 Arhgap44

Phylogenomic databases

eggNOGiKOG4270 Eukaryota
ENOG410XRR2 LUCA
GeneTreeiENSGT00760000118863
HOVERGENiHBG000015
InParanoidiQ5SSM3
KOiK21067
OrthoDBiEOG091G02YP
PhylomeDBiQ5SSM3
TreeFamiTF316514

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Miscellaneous databases

PROiPR:Q5SSM3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033389 Expressed in 234 organ(s), highest expression level in brain
CleanExiMM_AU040829
ExpressionAtlasiQ5SSM3 baseline and differential
GenevisibleiQ5SSM3 MM

Family and domain databases

Gene3Di1.10.555.10, 1 hit
1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR004148 BAR_dom
IPR008936 Rho_GTPase_activation_prot
IPR000198 RhoGAP_dom
PfamiView protein in Pfam
PF03114 BAR, 1 hit
PF00620 RhoGAP, 1 hit
SMARTiView protein in SMART
SM00721 BAR, 1 hit
SM00324 RhoGAP, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF48350 SSF48350, 1 hit
PROSITEiView protein in PROSITE
PS51021 BAR, 1 hit
PS50238 RHOGAP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRHG44_MOUSE
AccessioniPrimary (citable) accession number: Q5SSM3
Secondary accession number(s): Q5SSM4
, Q5SSM6, Q5SSM7, Q7TNB9, Q8BW90
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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