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Protein

Glycoprotein endo-alpha-1,2-mannosidase

Gene

MANEA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

GlcMan9GlcNAc2-[protein] + H2O = Man8GlcNAc2-[protein] (isomer 8A(1,2,3)B(1,2)) + alpha-D-glucosyl-(1->3)-alpha-D-mannopyranose.2 Publications

pH dependencei

Optimum pH is 6.2.1 Publication

Temperature dependencei

Optimum temperature is 37 degrees Celsius.1 Publication

GO - Molecular functioni

  • alpha-mannosidase activity Source: GO_Central
  • glycoprotein endo-alpha-1,2-mannosidase activity Source: Reactome

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BioCyciMetaCyc:HS16090-MONOMER
BRENDAi3.2.1.130 2681
ReactomeiR-HSA-964739 N-glycan trimming and elongation in the cis-Golgi

Protein family/group databases

CAZyiGH99 Glycoside Hydrolase Family 99

Names & Taxonomyi

Protein namesi
Recommended name:
Glycoprotein endo-alpha-1,2-mannosidase (EC:3.2.1.1302 Publications)
Short name:
Endo-alpha mannosidase
Short name:
Endomannosidase
Short name:
hEndo
Alternative name(s):
Mandaselin
Gene namesi
Name:MANEA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000172469.14
HGNCiHGNC:21072 MANEA
MIMi612327 gene
neXtProtiNX_Q5SRI9

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 29Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini30 – 462LumenalSequence analysisAdd BLAST433

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi79694
OpenTargetsiENSG00000172469
PharmGKBiPA134891001

Polymorphism and mutation databases

BioMutaiMANEA
DMDMi74743637

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002823151 – 462Glycoprotein endo-alpha-1,2-mannosidaseAdd BLAST462

Post-translational modificationi

Undergoes proteolytic cleavage in the C-terminal region.

Proteomic databases

EPDiQ5SRI9
MaxQBiQ5SRI9
PaxDbiQ5SRI9
PeptideAtlasiQ5SRI9
PRIDEiQ5SRI9
ProteomicsDBi63853

PTM databases

iPTMnetiQ5SRI9
PhosphoSitePlusiQ5SRI9
SwissPalmiQ5SRI9

Expressioni

Tissue specificityi

Highly expressed in the liver and kidney. Expressed at lower levels in muscle, pancreas, heart, placenta, lung and brain.1 Publication

Gene expression databases

BgeeiENSG00000172469 Expressed in 198 organ(s), highest expression level in kidney
CleanExiHS_MANEA
ExpressionAtlasiQ5SRI9 baseline and differential
GenevisibleiQ5SRI9 HS

Organism-specific databases

HPAiHPA011046
HPA011069

Interactioni

Protein-protein interaction databases

BioGridi122815, 26 interactors
STRINGi9606.ENSP00000351669

Structurei

3D structure databases

ProteinModelPortaliQ5SRI9
SMRiQ5SRI9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni60 – 462CatalyticCuratedAdd BLAST403

Sequence similaritiesi

Belongs to the glycosyl hydrolase 99 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IG22 Eukaryota
ENOG410XT8Q LUCA
GeneTreeiENSGT00390000016054
HOVERGENiHBG103563
InParanoidiQ5SRI9
KOiK15538
OMAiGFYSKLP
OrthoDBiEOG091G0CUF
PhylomeDBiQ5SRI9
TreeFamiTF324051

Family and domain databases

CDDicd11574 GH99, 1 hit
InterProiView protein in InterPro
IPR026071 Glyco_Hydrolase_99
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR13572 PTHR13572, 1 hit
PfamiView protein in Pfam
PF16317 Glyco_hydro_99, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q5SRI9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKFRRRTCI ILALFILFIF SLMMGLKMLR PNTATFGAPF GLDLLPELHQ
60 70 80 90 100
RTIHLGKNFD FQKSDRINSE TNTKNLKSVE ITMKPSKASE LNLDELPPLN
110 120 130 140 150
NYLHVFYYSW YGNPQFDGKY IHWNHPVLEH WDPRIAKNYP QGRHNPPDDI
160 170 180 190 200
GSSFYPELGS YSSRDPSVIE THMRQMRSAS IGVLALSWYP PDVNDENGEP
210 220 230 240 250
TDNLVPTILD KAHKYNLKVT FHIEPYSNRD DQNMYKNVKY IIDKYGNHPA
260 270 280 290 300
FYRYKTKTGN ALPMFYVYDS YITKPEKWAN LLTTSGSRSI RNSPYDGLFI
310 320 330 340 350
ALLVEEKHKY DILQSGFDGI YTYFATNGFT YGSSHQNWAS LKLFCDKYNL
360 370 380 390 400
IFIPSVGPGY IDTSIRPWNT QNTRNRINGK YYEIGLSAAL QTRPSLISIT
410 420 430 440 450
SFNEWHEGTQ IEKAVPKRTS NTVYLDYRPH KPGLYLELTR KWSEKYSKER
460
ATYALDRQLP VS
Length:462
Mass (Da):53,671
Last modified:December 21, 2004 - v1
Checksum:iCB593CB9B3DAA056
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
X6R7A2X6R7A2_HUMAN
Glycoprotein endo-alpha-1,2-mannosi...
MANEA
198Annotation score:

Sequence cautioni

The sequence AAL07306 differs from that shown. Reason: Frameshift at several positions.Curated
The sequence CAD97640 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti129E → G in CAD97640 (PubMed:17974005).Curated1
Sequence conflicti237N → S in CAE12165 (PubMed:15760709).Curated1
Sequence conflicti344F → I in AAQ75077 (PubMed:15677381).Curated1
Sequence conflicti344F → I in BAB14298 (PubMed:14702039).Curated1
Sequence conflicti344F → I in CAE45927 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_036242331Y → C in a breast cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs780723017Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ577574 mRNA Translation: CAE12165.1
AY372528 mRNA Translation: AAQ75077.1
AK022900 mRNA Translation: BAB14298.1
AK291940 mRNA Translation: BAF84629.1
BX537398 mRNA Translation: CAD97640.1 Different initiation.
BX640869 mRNA Translation: CAE45927.1
AL671884 Genomic DNA No translation available.
BC137014 mRNA Translation: AAI37015.1
BC137016 mRNA Translation: AAI37017.1
BC146671 mRNA Translation: AAI46672.1
BC150199 mRNA Translation: AAI50200.1
AY048774 mRNA Translation: AAL07306.1 Frameshift.
CCDSiCCDS5032.1
RefSeqiNP_078917.2, NM_024641.3
XP_005267204.1, XM_005267147.3
UniGeneiHs.734632
Hs.743412

Genome annotation databases

EnsembliENST00000358812; ENSP00000351669; ENSG00000172469
GeneIDi79694
KEGGihsa:79694
UCSCiuc003poo.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ577574 mRNA Translation: CAE12165.1
AY372528 mRNA Translation: AAQ75077.1
AK022900 mRNA Translation: BAB14298.1
AK291940 mRNA Translation: BAF84629.1
BX537398 mRNA Translation: CAD97640.1 Different initiation.
BX640869 mRNA Translation: CAE45927.1
AL671884 Genomic DNA No translation available.
BC137014 mRNA Translation: AAI37015.1
BC137016 mRNA Translation: AAI37017.1
BC146671 mRNA Translation: AAI46672.1
BC150199 mRNA Translation: AAI50200.1
AY048774 mRNA Translation: AAL07306.1 Frameshift.
CCDSiCCDS5032.1
RefSeqiNP_078917.2, NM_024641.3
XP_005267204.1, XM_005267147.3
UniGeneiHs.734632
Hs.743412

3D structure databases

ProteinModelPortaliQ5SRI9
SMRiQ5SRI9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi122815, 26 interactors
STRINGi9606.ENSP00000351669

Protein family/group databases

CAZyiGH99 Glycoside Hydrolase Family 99

PTM databases

iPTMnetiQ5SRI9
PhosphoSitePlusiQ5SRI9
SwissPalmiQ5SRI9

Polymorphism and mutation databases

BioMutaiMANEA
DMDMi74743637

Proteomic databases

EPDiQ5SRI9
MaxQBiQ5SRI9
PaxDbiQ5SRI9
PeptideAtlasiQ5SRI9
PRIDEiQ5SRI9
ProteomicsDBi63853

Protocols and materials databases

DNASUi79694
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000358812; ENSP00000351669; ENSG00000172469
GeneIDi79694
KEGGihsa:79694
UCSCiuc003poo.3 human

Organism-specific databases

CTDi79694
DisGeNETi79694
EuPathDBiHostDB:ENSG00000172469.14
GeneCardsiMANEA
HGNCiHGNC:21072 MANEA
HPAiHPA011046
HPA011069
MIMi612327 gene
neXtProtiNX_Q5SRI9
OpenTargetsiENSG00000172469
PharmGKBiPA134891001
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IG22 Eukaryota
ENOG410XT8Q LUCA
GeneTreeiENSGT00390000016054
HOVERGENiHBG103563
InParanoidiQ5SRI9
KOiK15538
OMAiGFYSKLP
OrthoDBiEOG091G0CUF
PhylomeDBiQ5SRI9
TreeFamiTF324051

Enzyme and pathway databases

BioCyciMetaCyc:HS16090-MONOMER
BRENDAi3.2.1.130 2681
ReactomeiR-HSA-964739 N-glycan trimming and elongation in the cis-Golgi

Miscellaneous databases

GeneWikiiMANEA
GenomeRNAii79694
PROiPR:Q5SRI9
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000172469 Expressed in 198 organ(s), highest expression level in kidney
CleanExiHS_MANEA
ExpressionAtlasiQ5SRI9 baseline and differential
GenevisibleiQ5SRI9 HS

Family and domain databases

CDDicd11574 GH99, 1 hit
InterProiView protein in InterPro
IPR026071 Glyco_Hydrolase_99
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR13572 PTHR13572, 1 hit
PfamiView protein in Pfam
PF16317 Glyco_hydro_99, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMANEA_HUMAN
AccessioniPrimary (citable) accession number: Q5SRI9
Secondary accession number(s): A6H8M6
, Q5SRJ0, Q6MZV0, Q70JE9, Q7Z3V7, Q8WWX5, Q9H9D2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  6. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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