UniProtKB - Q5SQI0 (ATAT_HUMAN)
Protein
Alpha-tubulin N-acetyltransferase 1
Gene
ATAT1
Organism
Homo sapiens (Human)
Status
Functioni
Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.UniRule annotation4 Publications
Catalytic activityi
- acetyl-CoA + L-lysyl-[α-tubulin] = CoA + H+ + N6-acetyl-L-lysyl-[α-tubulin]UniRule annotation4 PublicationsEC:2.3.1.108UniRule annotation4 Publications
Kineticsi
kcat is 2.2 h(-1) for the acetylation of polymerized tubulin (microtubules), 0.35 h(-1) for the acetylation of free tubulin, and 1.47 h(-1)with acetyl-CoA as substrate.
- KM=2.0 µM for free alpha-tubulin2 Publications
- KM=1.6 µM for polymerized tubulin (microtubules)2 Publications
- KM=2.2 µM for acetyl-CoA2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 58 | Crucial for catalytic activityUniRule annotation1 Publication | 1 |
GO - Molecular functioni
- lysine N-acetyltransferase activity, acting on acetyl phosphate as donor Source: UniProtKB
- tubulin N-acetyltransferase activity Source: MGI
GO - Biological processi
- alpha-tubulin acetylation Source: UniProtKB
- cilium assembly Source: Reactome
- dentate gyrus development Source: Ensembl
- neuron development Source: UniProtKB-UniRule
- positive regulation of NLRP3 inflammasome complex assembly Source: Ensembl
- regulation of fat cell differentiation Source: Ensembl
- regulation of microtubule cytoskeleton organization Source: UniProtKB-UniRule
- spermatogenesis Source: Ensembl
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
PathwayCommonsi | Q5SQI0 |
Reactomei | R-HSA-5617833, Cilium Assembly |
Names & Taxonomyi
Protein namesi | Recommended name: Alpha-tubulin N-acetyltransferase 1UniRule annotation (EC:2.3.1.108UniRule annotation3 Publications)Short name: Alpha-TATUniRule annotation Short name: Alpha-TAT1UniRule annotation Short name: TATUniRule annotation Alternative name(s): Acetyltransferase mec-17 homologUniRule annotation |
Gene namesi | ORF Names:Nbla00487 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:21186, ATAT1 |
MIMi | 615556, gene |
neXtProti | NX_Q5SQI0 |
VEuPathDBi | HostDB:ENSG00000137343.17 |
Subcellular locationi
Cytoskeleton
- cytoskeleton UniRule annotation
- spindle UniRule annotation
Other locations
- Cytoplasm UniRule annotation1 Publication
- clathrin-coated pit UniRule annotation1 Publication
- focal adhesion UniRule annotation1 Publication
- axon UniRule annotation
Cytoskeleton
- microtubule Source: InterPro
- microtubule bundle Source: Ensembl
- mitotic spindle Source: MGI
Cytosol
- cytosol Source: HPA
Golgi apparatus
- Golgi apparatus Source: HPA
Plasma Membrane
- clathrin-coated pit Source: UniProtKB-SubCell
Other locations
- axon Source: UniProtKB-SubCell
- focal adhesion Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell projection, Coated pit, Cytoplasm, Cytoskeleton, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 58 | Q → A: Loss of acetyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 61 | S → A: No effect on catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 64 | I → A: Strong reduction in acetyltransferase activity. 3 Publications | 1 | |
Mutagenesisi | 69 | R → A: Strong reduction in acetyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 102 | K → A: Strong reduction in acetyltransferase activity. 1 Publication | 1 | |
Mutagenesisi | 105 | F → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 106 | V → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 107 | L → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 108 | D → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 109 | D → A: Slight increase in activity. 1 Publication | 1 | |
Mutagenesisi | 109 | D → R: Marginal increase in activity. 1 Publication | 1 | |
Mutagenesisi | 111 | E → A: 2-fold increase in activity. 1 Publication | 1 | |
Mutagenesisi | 111 | E → R: No effect on catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 115 | E → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 117 | E → A: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 120 | C → A or S: Strong reduction in microtubule acetylation. 1 Publication | 1 | |
Mutagenesisi | 157 | D → E or N: Strong reduction in microtubule acetylation. 2 Publications | 1 | |
Mutagenesisi | 158 | R → A: 20% of wild-type acetyltransferase activity. 2 Publications | 1 | |
Mutagenesisi | 182 | N → A: Strong reduction in activity. 1 Publication | 1 | |
Mutagenesisi | 183 | F → A: Strong reduction in activity. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 79969 |
OpenTargetsi | ENSG00000137343 |
PharmGKBi | PA134948212 |
Miscellaneous databases
Pharosi | Q5SQI0, Tbio |
Genetic variation databases
BioMutai | ATAT1 |
DMDMi | 74743537 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000348066 | 1 – 421 | Alpha-tubulin N-acetyltransferase 1Add BLAST | 421 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 56 | N6-acetyllysine; by autocatalysisUniRule annotationBy similarity | 1 | |
Modified residuei | 146 | N6-acetyllysine; by autocatalysisUniRule annotationBy similarity | 1 | |
Modified residuei | 233 | N6-acetyllysine; by autocatalysisUniRule annotationBy similarity | 1 | |
Modified residuei | 244 | N6-acetyllysine; by autocatalysisUniRule annotationBy similarity | 1 | |
Modified residuei | 272 | PhosphoserineBy similarity | 1 | |
Modified residuei | 276 | PhosphoserineCombined sources | 1 | |
Modified residuei | 305 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 315 | PhosphoserineCombined sources | 1 | |
Modified residuei | 323 | Omega-N-methylarginineCombined sources | 1 |
Post-translational modificationi
Autoacetylation strongly increases tubulin acetylation.UniRule annotation
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | Q5SQI0 |
jPOSTi | Q5SQI0 |
MassIVEi | Q5SQI0 |
PaxDbi | Q5SQI0 |
PeptideAtlasi | Q5SQI0 |
PRIDEi | Q5SQI0 |
ProteomicsDBi | 63801 [Q5SQI0-1] 63802 [Q5SQI0-2] 63803 [Q5SQI0-3] 63804 [Q5SQI0-4] 63805 [Q5SQI0-5] 63806 [Q5SQI0-6] 63807 [Q5SQI0-7] |
PTM databases
iPTMneti | Q5SQI0 |
PhosphoSitePlusi | Q5SQI0 |
Expressioni
Gene expression databases
Bgeei | ENSG00000137343, Expressed in cortical plate and 180 other tissues |
ExpressionAtlasi | Q5SQI0, baseline and differential |
Genevisiblei | Q5SQI0, HS |
Organism-specific databases
HPAi | ENSG00000137343, Low tissue specificity |
Interactioni
Subunit structurei
Component of the BBSome complex.
Interacts with AP2 alpha-adaptins, including AP2A2, but not with AP1 gamma-adaptin (AP1G1/AP1G2); this interaction is required for efficient alpha-tubulin acetylation, hence clathrin-coated pits are sites of microtubule acetylation.
UniRule annotation4 PublicationsProtein-protein interaction databases
BioGRIDi | 123036, 10 interactors |
IntActi | Q5SQI0, 8 interactors |
STRINGi | 9606.ENSP00000327832 |
Miscellaneous databases
RNActi | Q5SQI0, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q5SQI0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 190 | N-acetyltransferaseUniRule annotationAdd BLAST | 190 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 124 – 137 | Acetyl-CoA bindingUniRule annotation3 PublicationsAdd BLAST | 14 | |
Regioni | 160 – 169 | Acetyl-CoA bindingUniRule annotation3 Publications | 10 |
Sequence similaritiesi
Belongs to the acetyltransferase ATAT1 family.UniRule annotation
Phylogenomic databases
eggNOGi | KOG4601, Eukaryota |
GeneTreei | ENSGT00390000008276 |
HOGENOMi | CLU_025013_0_0_1 |
InParanoidi | Q5SQI0 |
OMAi | HESRQRC |
PhylomeDBi | Q5SQI0 |
TreeFami | TF315643 |
Family and domain databases
HAMAPi | MF_03130, mec17, 1 hit |
InterProi | View protein in InterPro IPR038746, Atat IPR007965, GNAT_ATAT |
PANTHERi | PTHR12327, PTHR12327, 1 hit |
Pfami | View protein in Pfam PF05301, Acetyltransf_16, 1 hit |
PROSITEi | View protein in PROSITE PS51730, GNAT_ATAT, 1 hit |
s (7)i Sequence
Sequence statusi: Complete.
This entry describes 7 produced by isoformsialternative splicing. AlignAdd to basketIsoform 11 Publication (identifier: Q5SQI0-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEFPFDVDAL FPERITVLDQ HLRPPARRPG TTTPARVDLQ QQIMTIIDEL
60 70 80 90 100
GKASAKAQNL SAPITSASRM QSNRHVVYIL KDSSARPAGK GAIIGFIKVG
110 120 130 140 150
YKKLFVLDDR EAHNEVEPLC ILDFYIHESV QRHGHGRELF QYMLQKERVE
160 170 180 190 200
PHQLAIDRPS QKLLKFLNKH YNLETTVPQV NNFVIFEGFF AHQHRPPAPS
210 220 230 240 250
LRATRHSRAA AVDPTPAAPA RKLPPKRAEG DIKPYSSSDR EFLKVAVEPP
260 270 280 290 300
WPLNRAPRRA TPPAHPPPRS SSLGNSPERG PLRPFVPEQE LLRSLRLCPP
310 320 330 340 350
HPTARLLLAA DPGGSPAQRR RTRGTPPGLV AQSCCYSRHG GVNSSSPNTG
360 370 380 390 400
NQDSKQGEQE TKNRSASEEQ ALSQDGSGEK PMHTAPPQAP APPAQSWTVG
410 420
GDILNARFIR NLQERRSTRP W
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_052899 | 1 – 12 | Missing in isoform 2. 2 PublicationsAdd BLAST | 12 | |
Alternative sequenceiVSP_052900 | 13 – 36 | ERITV…TTPAR → MWLTWPFCFLTITLREEGVC HLES in isoform 2. 2 PublicationsAdd BLAST | 24 | |
Alternative sequenceiVSP_052901 | 195 – 218 | RPPAP…PTPAA → P in isoform 3, isoform 6 and isoform 7. 2 PublicationsAdd BLAST | 24 | |
Alternative sequenceiVSP_052902 | 323 – 333 | RGTPPGLVAQS → SSLPRSEESRY in isoform 4 and isoform 6. 3 PublicationsAdd BLAST | 11 | |
Alternative sequenceiVSP_052903 | 324 – 421 | Missing in isoform 5 and isoform 7. 3 PublicationsAdd BLAST | 98 | |
Alternative sequenceiVSP_052904 | 334 – 421 | Missing in isoform 4 and isoform 6. 3 PublicationsAdd BLAST | 88 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB075512 mRNA Translation: BAE45758.1 AK023220 mRNA Translation: BAB14472.1 AL662800 Genomic DNA No translation available. AL732442 Genomic DNA No translation available. AL845353 Genomic DNA No translation available. BX119957 Genomic DNA No translation available. BX908728 Genomic DNA No translation available. CR753328 Genomic DNA No translation available. CR759778 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03306.1 CH471081 Genomic DNA Translation: EAX03307.1 BC006105 mRNA Translation: AAH06105.1 BC047303 mRNA Translation: AAH47303.1 AL833858 mRNA Translation: CAD38717.1 |
CCDSi | CCDS4683.2 [Q5SQI0-4] CCDS54978.1 [Q5SQI0-2] CCDS59002.1 [Q5SQI0-6] CCDS83072.1 [Q5SQI0-5] CCDS83073.1 [Q5SQI0-7] |
RefSeqi | NP_001026892.1, NM_001031722.3 [Q5SQI0-2] NP_001177653.1, NM_001190724.2 NP_001241881.1, NM_001254952.2 [Q5SQI0-6] NP_001305691.1, NM_001318762.1 [Q5SQI0-7] NP_001305692.1, NM_001318763.1 [Q5SQI0-5] NP_079185.2, NM_024909.3 [Q5SQI0-4] |
Genome annotation databases
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB075512 mRNA Translation: BAE45758.1 AK023220 mRNA Translation: BAB14472.1 AL662800 Genomic DNA No translation available. AL732442 Genomic DNA No translation available. AL845353 Genomic DNA No translation available. BX119957 Genomic DNA No translation available. BX908728 Genomic DNA No translation available. CR753328 Genomic DNA No translation available. CR759778 Genomic DNA No translation available. CH471081 Genomic DNA Translation: EAX03306.1 CH471081 Genomic DNA Translation: EAX03307.1 BC006105 mRNA Translation: AAH06105.1 BC047303 mRNA Translation: AAH47303.1 AL833858 mRNA Translation: CAD38717.1 |
CCDSi | CCDS4683.2 [Q5SQI0-4] CCDS54978.1 [Q5SQI0-2] CCDS59002.1 [Q5SQI0-6] CCDS83072.1 [Q5SQI0-5] CCDS83073.1 [Q5SQI0-7] |
RefSeqi | NP_001026892.1, NM_001031722.3 [Q5SQI0-2] NP_001177653.1, NM_001190724.2 NP_001241881.1, NM_001254952.2 [Q5SQI0-6] NP_001305691.1, NM_001318762.1 [Q5SQI0-7] NP_001305692.1, NM_001318763.1 [Q5SQI0-5] NP_079185.2, NM_024909.3 [Q5SQI0-4] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3VWD | X-ray | 1.25 | A | 1-194 | [»] | |
3VWE | X-ray | 1.96 | A | 1-194 | [»] | |
4B5O | X-ray | 1.05 | A | 1-196 | [»] | |
4B5P | X-ray | 1.60 | A/B | 1-196 | [»] | |
4GS4 | X-ray | 2.11 | A | 2-236 | [»] | |
4IF5 | X-ray | 1.70 | A | 1-193 | [»] | |
4PK2 | X-ray | 1.35 | A | 1-194 | [»] | |
4PK3 | X-ray | 1.35 | A | 1-194 | [»] | |
4U9Y | X-ray | 2.20 | A | 1-194 | [»] | |
4U9Z | X-ray | 1.80 | A | 1-194 | [»] | |
SMRi | Q5SQI0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 123036, 10 interactors |
IntActi | Q5SQI0, 8 interactors |
STRINGi | 9606.ENSP00000327832 |
PTM databases
iPTMneti | Q5SQI0 |
PhosphoSitePlusi | Q5SQI0 |
Genetic variation databases
BioMutai | ATAT1 |
DMDMi | 74743537 |
Proteomic databases
EPDi | Q5SQI0 |
jPOSTi | Q5SQI0 |
MassIVEi | Q5SQI0 |
PaxDbi | Q5SQI0 |
PeptideAtlasi | Q5SQI0 |
PRIDEi | Q5SQI0 |
ProteomicsDBi | 63801 [Q5SQI0-1] 63802 [Q5SQI0-2] 63803 [Q5SQI0-3] 63804 [Q5SQI0-4] 63805 [Q5SQI0-5] 63806 [Q5SQI0-6] 63807 [Q5SQI0-7] |
Protocols and materials databases
ABCDi | Q5SQI0, 1 sequenced antibody |
Antibodypediai | 26411, 124 antibodies |
DNASUi | 79969 |
Genome annotation databases
Organism-specific databases
CTDi | 79969 |
DisGeNETi | 79969 |
GeneCardsi | ATAT1 |
HGNCi | HGNC:21186, ATAT1 |
HPAi | ENSG00000137343, Low tissue specificity |
MIMi | 615556, gene |
neXtProti | NX_Q5SQI0 |
OpenTargetsi | ENSG00000137343 |
PharmGKBi | PA134948212 |
VEuPathDBi | HostDB:ENSG00000137343.17 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4601, Eukaryota |
GeneTreei | ENSGT00390000008276 |
HOGENOMi | CLU_025013_0_0_1 |
InParanoidi | Q5SQI0 |
OMAi | HESRQRC |
PhylomeDBi | Q5SQI0 |
TreeFami | TF315643 |
Enzyme and pathway databases
PathwayCommonsi | Q5SQI0 |
Reactomei | R-HSA-5617833, Cilium Assembly |
Miscellaneous databases
BioGRID-ORCSi | 79969, 6 hits in 873 CRISPR screens |
ChiTaRSi | ATAT1, human |
GenomeRNAii | 79969 |
Pharosi | Q5SQI0, Tbio |
PROi | PR:Q5SQI0 |
RNActi | Q5SQI0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000137343, Expressed in cortical plate and 180 other tissues |
ExpressionAtlasi | Q5SQI0, baseline and differential |
Genevisiblei | Q5SQI0, HS |
Family and domain databases
HAMAPi | MF_03130, mec17, 1 hit |
InterProi | View protein in InterPro IPR038746, Atat IPR007965, GNAT_ATAT |
PANTHERi | PTHR12327, PTHR12327, 1 hit |
Pfami | View protein in Pfam PF05301, Acetyltransf_16, 1 hit |
PROSITEi | View protein in PROSITE PS51730, GNAT_ATAT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATAT_HUMAN | |
Accessioni | Q5SQI0Primary (citable) accession number: Q5SQI0 Secondary accession number(s): A2AB28 Q9H8X5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 10, 2021 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Uncharacterized protein families (UPF)
List of uncharacterized protein family (UPF) entries - Human chromosome 6
Human chromosome 6: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families