UniProtKB - Q5SQF8 (SP30L_MOUSE)
Protein
Histone deacetylase complex subunit SAP30L
Gene
Sap30l
Organism
Mus musculus (Mouse)
Status
Functioni
Functions as transcription repressor, probably via its interaction with histone deacetylase complexes. Involved in the functional recruitment of the class 1 Sin3-histone deacetylase complex (HDAC) to the nucleolus. Binds DNA, apparently without sequence-specificity, and bends bound double-stranded DNA. Binds phosphoinositol phosphates (phosphoinositol 3-phosphate, phosphoinositol 4-phosphate and phosphoinositol 5-phosphate) via the same basic sequence motif that mediates DNA binding and nuclear import.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 28 – 76 | AtypicalAdd BLAST | 49 |
GO - Molecular functioni
- DNA binding Source: UniProtKB
- histone binding Source: UniProtKB
- histone deacetylase activity Source: GO_Central
- non-sequence-specific DNA binding, bending Source: UniProtKB
- nucleosome binding Source: UniProtKB
- phosphatidylinositol-3-phosphate binding Source: UniProtKB
- phosphatidylinositol-4-phosphate binding Source: UniProtKB
- phosphatidylinositol-5-phosphate binding Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- negative regulation of transcription by RNA polymerase II Source: UniProtKB
- regulation of transcription, DNA-templated Source: GO_Central
Keywordsi
Molecular function | DNA-binding, Repressor |
Biological process | Transcription, Transcription regulation |
Ligand | Lipid-binding, Metal-binding, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Histone deacetylase complex subunit SAP30LAlternative name(s): Sin3 corepressor complex subunit SAP30L Sin3-associated protein p30-like |
Gene namesi | Name:Sap30l |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1354709 Sap30l |
Subcellular locationi
Nucleus
- nucleolus By similarity
Nucleus
- histone deacetylase complex Source: UniProtKB
- nucleolus Source: UniProtKB
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000309501 | 1 – 182 | Histone deacetylase complex subunit SAP30LAdd BLAST | 182 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Disulfide bondi | 28 ↔ 29 | Redox-activePROSITE-ProRule annotation | ||
Disulfide bondi | 37 ↔ 73 | Redox-activePROSITE-ProRule annotation | ||
Cross-linki | 48 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Modified residuei | 92 | PhosphoserineCombined sources | 1 | |
Modified residuei | 98 | PhosphoserineCombined sources | 1 | |
Cross-linki | 154 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 165 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 174 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Acetylation, Disulfide bond, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q5SQF8 |
MaxQBi | Q5SQF8 |
PaxDbi | Q5SQF8 |
PeptideAtlasi | Q5SQF8 |
PRIDEi | Q5SQF8 |
PTM databases
iPTMneti | Q5SQF8 |
PhosphoSitePlusi | Q5SQF8 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000020519 Expressed in 292 organ(s), highest expression level in primary oocyte |
Genevisiblei | Q5SQF8 MM |
Interactioni
Subunit structurei
Interacts with components of the histone deacetylase complex SIN3A, HDAC1 and HDAC2. Binds histones and nucleosomes.
Interacts with FEZ1.
By similarityGO - Molecular functioni
- histone binding Source: UniProtKB
Protein-protein interaction databases
ComplexPortali | CPX-3441 SIN3A histone deacetylase complex, ES cell-specific variant CPX-3443 SIN3A histone deacetylase complex CPX-3444 SIN3B histone deacetylase complex |
IntActi | Q5SQF8, 1 interactor |
MINTi | Q5SQF8 |
STRINGi | 10090.ENSMUSP00000020826 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 87 – 89 | Important for DNA and phosphoinositide bindingBy similarity | 3 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 85 – 90 | Nuclear localization signal (NLS)By similarity | 6 |
Domaini
The zinc-finger domain mediates direct interaction with DNA and phosphoinositol phosphates (phosphoinositol 3-phosphate, phosphoinositol 4-phosphate and phosphoinositol 5-phosphate). In vitro oxydation causes reversible disulfide bond formation between Cys residues in the zinc-finger domain and reversible loss of zinc ion binding.By similarity
Sequence similaritiesi
Belongs to the SAP30 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 28 – 76 | AtypicalAdd BLAST | 49 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | ENOG410IHMG Eukaryota ENOG4111GVC LUCA |
GeneTreei | ENSGT00390000006633 |
HOGENOMi | HOG000007811 |
InParanoidi | Q5SQF8 |
OMAi | YKVSTRP |
OrthoDBi | 1520155at2759 |
PhylomeDBi | Q5SQF8 |
TreeFami | TF324135 |
Family and domain databases
Gene3Di | 1.10.720.110, 1 hit |
InterProi | View protein in InterPro IPR024145 His_deAcase_SAP30/SAP30L IPR038291 SAP30_C_sf IPR025718 SAP30_Sin3-bd IPR025717 SAP30_zn-finger |
PANTHERi | PTHR13286 PTHR13286, 1 hit |
Pfami | View protein in Pfam PF13867 SAP30_Sin3_bdg, 1 hit PF13866 zf-SAP30, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5SQF8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNGFSTEEDS REGPPAAPAA APGYGQSCCL IADGERCVRP AGNASFSKRV
60 70 80 90 100
QKSISQKKLK LDIDKSVRHL YICDFHKNFI QSVRNKRKRK ASDDGGDSPE
110 120 130 140 150
HDADIPEVDL FQLQVNTLRR YKRHYKLQTR PGFNKAQLAE TVSRHFRNIP
160 170 180
VNEKETLAYF IYMVKSNRSR LDQKSEGSKQ LE
Sequence cautioni
The sequence AAH94930 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL732587 Genomic DNA No translation available. BC051686 mRNA Translation: AAH51686.1 BC094930 mRNA Translation: AAH94930.1 Different initiation. BC117029 mRNA Translation: AAI17030.1 BC117031 mRNA Translation: AAI17032.1 AK010234 mRNA Translation: BAB26785.1 |
CCDSi | CCDS36160.1 |
RefSeqi | NP_001074637.1, NM_001081168.1 |
Genome annotation databases
Ensembli | ENSMUST00000020826; ENSMUSP00000020826; ENSMUSG00000020519 |
GeneIDi | 50724 |
KEGGi | mmu:50724 |
UCSCi | uc007jae.1 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL732587 Genomic DNA No translation available. BC051686 mRNA Translation: AAH51686.1 BC094930 mRNA Translation: AAH94930.1 Different initiation. BC117029 mRNA Translation: AAI17030.1 BC117031 mRNA Translation: AAI17032.1 AK010234 mRNA Translation: BAB26785.1 |
CCDSi | CCDS36160.1 |
RefSeqi | NP_001074637.1, NM_001081168.1 |
3D structure databases
SMRi | Q5SQF8 |
ModBasei | Search... |
Protein-protein interaction databases
ComplexPortali | CPX-3441 SIN3A histone deacetylase complex, ES cell-specific variant CPX-3443 SIN3A histone deacetylase complex CPX-3444 SIN3B histone deacetylase complex |
IntActi | Q5SQF8, 1 interactor |
MINTi | Q5SQF8 |
STRINGi | 10090.ENSMUSP00000020826 |
PTM databases
iPTMneti | Q5SQF8 |
PhosphoSitePlusi | Q5SQF8 |
Proteomic databases
EPDi | Q5SQF8 |
MaxQBi | Q5SQF8 |
PaxDbi | Q5SQF8 |
PeptideAtlasi | Q5SQF8 |
PRIDEi | Q5SQF8 |
Genome annotation databases
Ensembli | ENSMUST00000020826; ENSMUSP00000020826; ENSMUSG00000020519 |
GeneIDi | 50724 |
KEGGi | mmu:50724 |
UCSCi | uc007jae.1 mouse |
Organism-specific databases
CTDi | 79685 |
MGIi | MGI:1354709 Sap30l |
Phylogenomic databases
eggNOGi | ENOG410IHMG Eukaryota ENOG4111GVC LUCA |
GeneTreei | ENSGT00390000006633 |
HOGENOMi | HOG000007811 |
InParanoidi | Q5SQF8 |
OMAi | YKVSTRP |
OrthoDBi | 1520155at2759 |
PhylomeDBi | Q5SQF8 |
TreeFami | TF324135 |
Miscellaneous databases
ChiTaRSi | Sap30l mouse |
PROi | PR:Q5SQF8 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000020519 Expressed in 292 organ(s), highest expression level in primary oocyte |
Genevisiblei | Q5SQF8 MM |
Family and domain databases
Gene3Di | 1.10.720.110, 1 hit |
InterProi | View protein in InterPro IPR024145 His_deAcase_SAP30/SAP30L IPR038291 SAP30_C_sf IPR025718 SAP30_Sin3-bd IPR025717 SAP30_zn-finger |
PANTHERi | PTHR13286 PTHR13286, 1 hit |
Pfami | View protein in Pfam PF13867 SAP30_Sin3_bdg, 1 hit PF13866 zf-SAP30, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SP30L_MOUSE | |
Accessioni | Q5SQF8Primary (citable) accession number: Q5SQF8 Secondary accession number(s): Q504M9, Q9CUZ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | November 13, 2019 | |
This is version 105 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot