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Protein

PHD finger protein 12

Gene

Phf12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a transcriptional repressor. Involved in recruitment of functional SIN3A complexes to DNA. Represses transcription at least in part through the activity of an associated histone deacetylase (HDAC). May also repress transcription in a SIN3A-independent manner through recruitment of functional AES complexes to DNA (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri56 – 105PHD-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri271 – 321PHD-type 2; atypicalPROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
PHD finger protein 12
Alternative name(s):
PHD factor 1
Short name:
Pf1
Gene namesi
Name:Phf12Imported
Synonyms:Kiaa1523
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1924057 Phf12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000593031 – 1003PHD finger protein 12Add BLAST1003

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei131PhosphoserineCombined sources1
Modified residuei134PhosphoserineBy similarity1
Cross-linki467Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei555PhosphoserineBy similarity1
Modified residuei557PhosphothreonineBy similarity1
Modified residuei570PhosphothreonineBy similarity1
Modified residuei670PhosphothreonineCombined sources1
Cross-linki899Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki972Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki986Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki990Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ5SPL2
PaxDbiQ5SPL2
PRIDEiQ5SPL2

PTM databases

iPTMnetiQ5SPL2
PhosphoSitePlusiQ5SPL2

Expressioni

Tissue specificityi

Expressed mainly in heart, brain, lung, liver and testis.1 Publication

Developmental stagei

Expression is low in day 7 embryos, peaks at day 11 and declines through to day 17.1 Publication

Gene expression databases

BgeeiENSMUSG00000037791 Expressed in 273 organ(s), highest expression level in spleen
CleanExiMM_PHF12
ExpressionAtlasiQ5SPL2 baseline and differential
GenevisibleiQ5SPL2 MM

Interactioni

Subunit structurei

Interacts with SIN3A in a complex composed of HDAC1, SAP30 and SIN3A. Interacts with AES (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MORF4L1Q9UBU8-22EBI-15963335,EBI-10288852From a different organism.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi234499, 4 interactors
DIPiDIP-59633N
IntActiQ5SPL2, 1 interactor
STRINGi10090.ENSMUSP00000044990

Structurei

3D structure databases

ProteinModelPortaliQ5SPL2
SMRiQ5SPL2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini814 – 868FHAPROSITE-ProRule annotationAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi893 – 896Poly-ArgSequence analysis4
Compositional biasi928 – 931Poly-SerSequence analysis4

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri56 – 105PHD-type 1PROSITE-ProRule annotationAdd BLAST50
Zinc fingeri271 – 321PHD-type 2; atypicalPROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4299 Eukaryota
ENOG410XQQA LUCA
GeneTreeiENSGT00920000149099
HOGENOMiHOG000220810
HOVERGENiHBG057884
InParanoidiQ5SPL2
OMAiQWLCHSC
OrthoDBiEOG091G055U
PhylomeDBiQ5SPL2
TreeFamiTF336193

Family and domain databases

CDDicd00060 FHA, 1 hit
Gene3Di1.10.10.1860, 1 hit
1.20.920.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR036427 Bromodomain-like_sf
IPR000253 FHA_dom
IPR031966 PHF12_MRG-bd
IPR038098 PHF12_MRG-bd_sf
IPR008984 SMAD_FHA_dom_sf
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00628 PHD, 2 hits
PF16737 PHF12_MRG_bd, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SUPFAMiSSF49879 SSF49879, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 1 hit
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5SPL2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MWEKMETKTI VYDLDTSGGL MEQIQALLAP PKTDEAEKRS RKPEKESRRS
60 70 80 90 100
GRATNHDSCD SCKEGGDLLC CDHCPAAFHL QCCNPPLSEE MLPPGEWMCH
110 120 130 140 150
RCTVRRKKRE QKKELGHVNG LVDKSSKRTT SPSSDTDLLD RPASKTELKA
160 170 180 190 200
IAHARILERR ASRPGTPTSN ASTETPTSEH NDVDEDIVDV DEEPVAAEPD
210 220 230 240 250
YVQPQLRRPF ELLIAAAMER NPTQFQLPNE LTCTTALPGS SKRRRKEETT
260 270 280 290 300
GKNVKRTQHE LDHNGLVPLP VKVCFTCNRS CRVAPLIQCD YCPLLFHMDC
310 320 330 340 350
LEPPLTAMPL GRWMCPNHIE HVVLNQKNLT LSNRCQVFDR FQDTISQHVV
360 370 380 390 400
KVDFLNRIHK KHPPNRRVLQ SVKRRSLKVP DAIKSQYQFP PPLIAPAAIR
410 420 430 440 450
DGELICSGVP EESQTHLLNS EHLATQAEQQ EWLCSVVALQ CSILKHLSAK
460 470 480 490 500
QMPSPWDSEQ TEKADIKPVI VTDSSITTSL QTADKAPLPS HYPLSCPSAV
510 520 530 540 550
STQNSLGCSP PHQPPTLEDI SCSSCVEKSK KAPCGTANGP VNTEIKANGP
560 570 580 590 600
HLYSSPTDST DPRRLPGANT PLPGLTHRQG WPRPLTPPSA GGLQNHVGII
610 620 630 640 650
VKTENATGPS SCPQRSLVPV PSLPPSIPSS CASIENTSTL HRKTVQSQIG
660 670 680 690 700
PSSTESRPLG SPPNATRVLT PPQAAGDSIL ATGANQRFCS PAPSSDGKVS
710 720 730 740 750
PGTLSIGSAL TVPSFPANST AMVDLTNSLR AFMDVNGEIE INMLDEKLIK
760 770 780 790 800
FLALQRVHQL FPSRVQASPG NVGTHPLASG GHHPEVQRKE VQARAVFCPL
810 820 830 840 850
LGLGGAVNMC YRTLYIGTGA DMDVCLTNYG HCNYVSGKHA CIFYDENTKH
860 870 880 890 900
YELLNYSEHG TTVDNVLYSC DFSEKTPPTP PSSIVAKVQS VIRRRRHQKQ
910 920 930 940 950
DEEPSEEAAM MSSQAQGPQR RPCNCKASSS SLIGGSGAGW EGTALLHHGS
960 970 980 990 1000
YIKLGCLQFV FSITEFATKQ PKGDASLLQD GVLAEKLSLK PHQGPVLRSN

SVP
Length:1,003
Mass (Da):109,509
Last modified:December 21, 2004 - v1
Checksum:i915622923FA3EBF7
GO
Isoform 2 (identifier: Q5SPL2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     739-788: IEINMLDEKL...SGGHHPEVQR → STSAFPFPGP...PSALLSDVCE
     789-1003: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:788
Mass (Da):85,930
Checksum:i41B9C4C54DC99B97
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5SS87Q5SS87_MOUSE
PHD finger protein 12
Phf12
278Annotation score:
F6U3I4F6U3I4_MOUSE
PHD finger protein 12
Phf12
50Annotation score:
F7AXU7F7AXU7_MOUSE
PHD finger protein 12
Phf12
73Annotation score:
F7AKS4F7AKS4_MOUSE
PHD finger protein 12
Phf12
43Annotation score:

Sequence cautioni

The sequence AAH43080 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_051773739 – 788IEINM…PEVQR → STSAFPFPGPSFTGQCWDTS AGFWRAPPRRCARVHMPATT PSALLSDVCE in isoform 2. CuratedAdd BLAST50
Alternative sequenceiVSP_051774789 – 1003Missing in isoform 2. CuratedAdd BLAST215

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL669840, AL845484 Genomic DNA Translation: CAI25700.1
AL669840, AL845484 Genomic DNA Translation: CAI25701.1
AL845484, AL669840 Genomic DNA Translation: CAI25223.1
AL845484, AL669840 Genomic DNA Translation: CAI25224.1
BC043080 mRNA Translation: AAH43080.1 Different initiation.
AK129383 Transcribed RNA Translation: BAC98193.2
CCDSiCCDS48855.1 [Q5SPL2-1]
RefSeqiNP_777277.2, NM_174852.3 [Q5SPL2-1]
UniGeneiMm.274329

Genome annotation databases

EnsembliENSMUST00000049167; ENSMUSP00000044990; ENSMUSG00000037791 [Q5SPL2-1]
ENSMUST00000108360; ENSMUSP00000103997; ENSMUSG00000037791 [Q5SPL2-2]
GeneIDi268448
KEGGimmu:268448
UCSCiuc007kht.2 mouse [Q5SPL2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL669840, AL845484 Genomic DNA Translation: CAI25700.1
AL669840, AL845484 Genomic DNA Translation: CAI25701.1
AL845484, AL669840 Genomic DNA Translation: CAI25223.1
AL845484, AL669840 Genomic DNA Translation: CAI25224.1
BC043080 mRNA Translation: AAH43080.1 Different initiation.
AK129383 Transcribed RNA Translation: BAC98193.2
CCDSiCCDS48855.1 [Q5SPL2-1]
RefSeqiNP_777277.2, NM_174852.3 [Q5SPL2-1]
UniGeneiMm.274329

3D structure databases

ProteinModelPortaliQ5SPL2
SMRiQ5SPL2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi234499, 4 interactors
DIPiDIP-59633N
IntActiQ5SPL2, 1 interactor
STRINGi10090.ENSMUSP00000044990

PTM databases

iPTMnetiQ5SPL2
PhosphoSitePlusiQ5SPL2

Proteomic databases

EPDiQ5SPL2
PaxDbiQ5SPL2
PRIDEiQ5SPL2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000049167; ENSMUSP00000044990; ENSMUSG00000037791 [Q5SPL2-1]
ENSMUST00000108360; ENSMUSP00000103997; ENSMUSG00000037791 [Q5SPL2-2]
GeneIDi268448
KEGGimmu:268448
UCSCiuc007kht.2 mouse [Q5SPL2-1]

Organism-specific databases

CTDi57649
MGIiMGI:1924057 Phf12
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4299 Eukaryota
ENOG410XQQA LUCA
GeneTreeiENSGT00920000149099
HOGENOMiHOG000220810
HOVERGENiHBG057884
InParanoidiQ5SPL2
OMAiQWLCHSC
OrthoDBiEOG091G055U
PhylomeDBiQ5SPL2
TreeFamiTF336193

Miscellaneous databases

ChiTaRSiPhf12 mouse
PROiPR:Q5SPL2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000037791 Expressed in 273 organ(s), highest expression level in spleen
CleanExiMM_PHF12
ExpressionAtlasiQ5SPL2 baseline and differential
GenevisibleiQ5SPL2 MM

Family and domain databases

CDDicd00060 FHA, 1 hit
Gene3Di1.10.10.1860, 1 hit
1.20.920.10, 1 hit
3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR036427 Bromodomain-like_sf
IPR000253 FHA_dom
IPR031966 PHF12_MRG-bd
IPR038098 PHF12_MRG-bd_sf
IPR008984 SMAD_FHA_dom_sf
IPR019786 Zinc_finger_PHD-type_CS
IPR011011 Znf_FYVE_PHD
IPR001965 Znf_PHD
IPR019787 Znf_PHD-finger
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF00628 PHD, 2 hits
PF16737 PHF12_MRG_bd, 1 hit
SMARTiView protein in SMART
SM00249 PHD, 2 hits
SUPFAMiSSF49879 SSF49879, 1 hit
SSF57903 SSF57903, 2 hits
PROSITEiView protein in PROSITE
PS50006 FHA_DOMAIN, 1 hit
PS01359 ZF_PHD_1, 1 hit
PS50016 ZF_PHD_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiPHF12_MOUSE
AccessioniPrimary (citable) accession number: Q5SPL2
Secondary accession number(s): Q5SPL3, Q6ZPN8, Q80W50
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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