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Protein

Phosphoenolpyruvate carboxykinase (ATP)

Gene

pckA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA (By similarity).By similarity1 Publication

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Activity regulationi

Allosterically activated by calcium.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52SubstrateUniRule annotation1
Metal bindingi130Calcium; via carbonyl oxygen1
Metal bindingi131Calcium; via carbonyl oxygen1
Metal bindingi133Calcium; via carbonyl oxygen1
Binding sitei191SubstrateUniRule annotation1
Metal bindingi197ManganeseUniRule annotation1
Binding sitei197ATPUniRule annotation1
Binding sitei197SubstrateUniRule annotation1
Metal bindingi216Manganese 2; via tele nitrogenUniRule annotation1
Binding sitei216ATPUniRule annotation1
Metal bindingi253ManganeseUniRule annotation1
Metal bindingi267Calcium; via carbonyl oxygen1
Binding sitei281ATPUniRule annotation1
Binding sitei319ATPUniRule annotation1
Binding sitei319SubstrateUniRule annotation1
Binding sitei439ATP; via carbonyl oxygenUniRule annotation2 Publications1
Binding sitei444ATPUniRule annotation2 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi232 – 240ATPUniRule annotation2 Publications9
Nucleotide bindingi438 – 439ATP2 Publications2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandATP-binding, Calcium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-291-MONOMER
BRENDAi4.1.1.49 2305
UniPathwayi
UPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase (ATP)UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Synonyms:tthHB8IM
Ordered Locus Names:TTHA0278
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002369521 – 529Phosphoenolpyruvate carboxykinase (ATP)Add BLAST529

Interactioni

Subunit structurei

Dimer of dimers.2 Publications

Protein-protein interaction databases

STRINGi300852.TTHA0278

Structurei

Secondary structure

1529
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5SLL5
SMRiQ5SLL5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SLL5

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase (ATP) family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DJ1 Bacteria
COG1866 LUCA
HOGENOMiHOG000271471
KOiK01610
OMAiMRYAGEM
PhylomeDBiQ5SLL5

Family and domain databases

CDDicd00484 PEPCK_ATP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00453 PEPCK_ATP, 1 hit
InterProiView protein in InterPro
IPR001272 PEP_carboxykinase_ATP
IPR013035 PEP_carboxykinase_C
IPR008210 PEP_carboxykinase_N
IPR015994 PEPCK_ATP_CS
PANTHERiPTHR30031 PTHR30031, 1 hit
PfamiView protein in Pfam
PF01293 PEPCK_ATP, 1 hit
PIRSFiPIRSF006294 PEP_crbxkin, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
TIGRFAMsiTIGR00224 pckA, 1 hit
PROSITEiView protein in PROSITE
PS00532 PEPCK_ATP, 1 hit

Sequencei

Sequence statusi: Complete.

Q5SLL5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MQRLEALGIH PKKRVFWNTV SPVLVEHTLL RGEGLLAHHG PLVVDTTPYT
60 70 80 90 100
GRSPKDKFVV REPEVEGEIW WGEVNQPFAP EAFEALYQRV VQYLSERDLY
110 120 130 140 150
VQDLYAGADR RYRLAVRVVT ESPWHALFAR NMFILPRRFG NDDEVEAFVP
160 170 180 190 200
GFTVVHAPYF QAVPERDGTR SEVFVGISFQ RRLVLIVGTK YAGEIKKSIF
210 220 230 240 250
TVMNYLMPKR GVFPMHASAN VGKEGDVAVF FGLSGTGKTT LSTDPERPLI
260 270 280 290 300
GDDEHGWSED GVFNFEGGCY AKVIRLSPEH EPLIYKASNQ FEAILENVVV
310 320 330 340 350
NPESRRVQWD DDSKTENTRS SYPIAHLENV VESGVAGHPR AIFFLSADAY
360 370 380 390 400
GVLPPIARLS PEEAMYYFLS GYTARVAGTE RGVTEPRATF SACFGAPFLP
410 420 430 440 450
MHPGVYARML GEKIRKHAPR VYLVNTGWTG GPYGVGYRFP LPVTRALLKA
460 470 480 490 500
ALSGALENVP YRRDPVFGFE VPLEAPGVPQ ELLNPRETWA DKEAYDQQAR
510 520
KLARLFQENF QKYASGVAKE VAEAGPRTE
Length:529
Mass (Da):59,314
Last modified:December 21, 2004 - v1
Checksum:i62FC60C17D719D70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA Translation: BAD70101.1
RefSeqiWP_011227826.1, NC_006461.1
YP_143544.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD70101; BAD70101; BAD70101
GeneIDi3168667
KEGGittj:TTHA0278
PATRICifig|300852.9.peg.278

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA Translation: BAD70101.1
RefSeqiWP_011227826.1, NC_006461.1
YP_143544.1, NC_006461.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J3BX-ray2.00A/B1-529[»]
1XKVX-ray2.20A/B1-529[»]
2PC9X-ray2.40A/B/C/D1-529[»]
ProteinModelPortaliQ5SLL5
SMRiQ5SLL5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA0278

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD70101; BAD70101; BAD70101
GeneIDi3168667
KEGGittj:TTHA0278
PATRICifig|300852.9.peg.278

Phylogenomic databases

eggNOGiENOG4105DJ1 Bacteria
COG1866 LUCA
HOGENOMiHOG000271471
KOiK01610
OMAiMRYAGEM
PhylomeDBiQ5SLL5

Enzyme and pathway databases

UniPathwayi
UPA00138

BioCyciTTHE300852:G1GKC-291-MONOMER
BRENDAi4.1.1.49 2305

Miscellaneous databases

EvolutionaryTraceiQ5SLL5

Family and domain databases

CDDicd00484 PEPCK_ATP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00453 PEPCK_ATP, 1 hit
InterProiView protein in InterPro
IPR001272 PEP_carboxykinase_ATP
IPR013035 PEP_carboxykinase_C
IPR008210 PEP_carboxykinase_N
IPR015994 PEPCK_ATP_CS
PANTHERiPTHR30031 PTHR30031, 1 hit
PfamiView protein in Pfam
PF01293 PEPCK_ATP, 1 hit
PIRSFiPIRSF006294 PEP_crbxkin, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
TIGRFAMsiTIGR00224 pckA, 1 hit
PROSITEiView protein in PROSITE
PS00532 PEPCK_ATP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_THET8
AccessioniPrimary (citable) accession number: Q5SLL5
Secondary accession number(s): P84128, Q7SIC6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: December 21, 2004
Last modified: October 10, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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