Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type-4 uracil-DNA glycosylase

Gene

udg

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Removes uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP. Can remove uracil from double-stranded DNA containing either a U/G, U/A, U/C or U/T base pair as well as from single-stranded DNA (PubMed:12000829, PubMed:14556741). Specifically recognizes uracil that is flipped out from double-stranded DNA (PubMed:14556741).2 Publications

Miscellaneous

The 4Fe-4S cluster may have a structural rather than a catalytic role. Does not appear to be essential for enzyme activity.1 Publication

Catalytic activityi

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.2 Publications

Enzyme regulationi

Product-inhibited by apurinic/apyrimidinic sites.1 Publication

Kineticsi

  1. KM=1.0 µM for G/U dsDNA1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi13Iron-sulfur (4Fe-4S)Combined sources1 Publication1
    Metal bindingi16Iron-sulfur (4Fe-4S)Combined sources1 Publication1
    Binding sitei54Uracil; via amide nitrogen1 Publication1
    Binding sitei80Uracil1 Publication1
    Metal bindingi84Iron-sulfur (4Fe-4S)Combined sources1 Publication1
    Metal bindingi100Iron-sulfur (4Fe-4S)Combined sources1 Publication1
    Binding sitei155Uracil1 Publication1

    GO - Molecular functioni

    • 4 iron, 4 sulfur cluster binding Source: UniProtKB
    • metal ion binding Source: UniProtKB-KW
    • uracil DNA N-glycosylase activity Source: UniProtKB

    GO - Biological processi

    • DNA repair Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase
    Biological processDNA damage, DNA repair
    Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.2.2.27 2305

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-4 uracil-DNA glycosylaseCurated (EC:3.2.2.272 Publications)
    Alternative name(s):
    TTUDGA1 Publication
    TthUDG1 Publication
    Gene namesi
    Name:udgImported
    Ordered Locus Names:TTHA0718Imported
    OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
    Taxonomic identifieri300852 [NCBI]
    Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
    Proteomesi
    • UP000000532 Componenti: Chromosome

    Pathology & Biotechi

    Chemistry databases

    DrugBankiDB03419 Uracil

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004391821 – 205Type-4 uracil-DNA glycosylaseAdd BLAST205

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi300852.TTHA0718

    Structurei

    Secondary structure

    1205
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Helixi3 – 10Combined sources8
    Helixi17 – 19Combined sources3
    Beta strandi35 – 41Combined sources7
    Helixi45 – 50Combined sources6
    Helixi57 – 69Combined sources13
    Helixi73 – 75Combined sources3
    Beta strandi76 – 81Combined sources6
    Helixi87 – 89Combined sources3
    Helixi94 – 103Combined sources10
    Helixi105 – 112Combined sources8
    Beta strandi115 – 121Combined sources7
    Helixi122 – 129Combined sources8
    Helixi135 – 138Combined sources4
    Beta strandi143 – 145Combined sources3
    Beta strandi148 – 150Combined sources3
    Helixi156 – 161Combined sources6
    Helixi170 – 187Combined sources18

    3D structure databases

    ProteinModelPortaliQ5SKC5
    SMRiQ5SKC5
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni40 – 42Uracil binding1 Publication3

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4108UN4 Bacteria
    COG1573 LUCA
    HOGENOMiHOG000196501
    KOiK21929
    OMAiWLMWQDI
    PhylomeDBiQ5SKC5

    Family and domain databases

    CDDicd10030 UDG_F4_TTUDGA_like, 1 hit
    Gene3Di3.40.470.10, 1 hit
    InterProiView protein in InterPro
    IPR005273 Ura-DNA_glyco_family4
    IPR005122 Uracil-DNA_glycosylase-like
    IPR036895 Uracil-DNA_glycosylase-like_sf
    PfamiView protein in Pfam
    PF03167 UDG, 1 hit
    SMARTiView protein in SMART
    SM00986 UDG, 1 hit
    SUPFAMiSSF52141 SSF52141, 1 hit
    TIGRFAMsiTIGR00758 UDG_fam4, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q5SKC5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTLELLQAQA QNCTACRLME GRTRVVFGEG NPDAKLMIVG EGPGEEEDKT
    60 70 80 90 100
    GRPFVGKAGQ LLNRILEAAG IPREEVYITN IVKCRPPQNR APLPDEAKIC
    110 120 130 140 150
    TDKWLLKQIE LIAPQIIVPL GAVAAEFFLG EKVSITKVRG KWYEWHGIKV
    160 170 180 190 200
    FPMFHPAYLL RNPSRAPGSP KHLTWLDIQE VKRALDALPP KERRPVKAVS

    QEPLF
    Length:205
    Mass (Da):22,966
    Last modified:December 21, 2004 - v1
    Checksum:i74720211D889806F
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AB109239 Genomic DNA Translation: BAC79245.1
    AP008226 Genomic DNA Translation: BAD70541.1
    RefSeqiWP_011228142.1, NC_006461.1
    YP_143984.1, NC_006461.1

    Genome annotation databases

    EnsemblBacteriaiBAD70541; BAD70541; BAD70541
    GeneIDi3169183
    KEGGittj:TTHA0718
    PATRICifig|300852.9.peg.711

    Similar proteinsi

    Entry informationi

    Entry nameiUDGA_THET8
    AccessioniPrimary (citable) accession number: Q5SKC5
    Secondary accession number(s): Q7WYV4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2017
    Last sequence update: December 21, 2004
    Last modified: February 28, 2018
    This is version 66 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

    Do not show this banner again
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health