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Protein

Ribosomal RNA small subunit methyltransferase F

Gene

rsmF

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates the cytosines at positions 1400 (m5C1400), 1404 (m5C1404) and 1407 (m5C1407) of 16S rRNA. C1400, C1404 and C1407 are methylated in a 30S subunit substrate, but only C1400 and C1404 are methylated when naked 16S rRNA is the substrate. Methylation by RsmF may facilitate growth at temperatures outside the optimal growth temperature.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + cytosine(1400) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1400) in 16S rRNA.1 Publication
S-adenosyl-L-methionine + cytosine(1404) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1404) in 16S rRNA.1 Publication
S-adenosyl-L-methionine + cytosine(1407) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1407) in 16S rRNA.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133S-adenosyl-L-methionine1 Publication1
Binding sitei138S-adenosyl-L-methionine1 Publication1
Binding sitei177S-adenosyl-L-methionine1 Publication1
Active sitei230NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processrRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-1392-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase FBy similarity (EC:2.1.1.-1 Publication, EC:2.1.1.1781 Publication)
Alternative name(s):
rRNA (cytosine-C(5)-)-methyltransferase RsmFBy similarity
Gene namesi
Name:rsmF1 Publication
Ordered Locus Names:TTHA1387Imported
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB02899 N-Carboxymethionine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004314811 – 456Ribosomal RNA small subunit methyltransferase FAdd BLAST456

Interactioni

Protein-protein interaction databases

STRINGi300852.TTHA1387

Structurei

Secondary structure

1456
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5SII2
SMRiQ5SII2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SII2

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 115S-adenosyl-L-methionine binding1 Publication7

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105CYJ Bacteria
COG0144 LUCA
HOGENOMiHOG000218116
OMAiDPLFHAG
PhylomeDBiQ5SII2

Family and domain databases

InterProiView protein in InterPro
IPR031341 Methyltr_RsmF_N
IPR001678 MeTrfase_RsmB/NOP2
IPR023267 RCMT
IPR031340 RsmF_methylt_CI
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PF17125 Methyltr_RsmF_N, 1 hit
PF17126 RsmF_methylt_CI, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit

Sequencei

Sequence statusi: Complete.

Q5SII2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLPKAFLSRM AELLGEEFPA FLKALTEGKR TYGLRVNTLK LPPEAFQRIS
60 70 80 90 100
PWPLRPIPWC QEGFYYPEEA RPGPHPFFYA GLYYIQEPSA QAVGVLLDPK
110 120 130 140 150
PGERVLDLAA APGGKTTHLA ARMGGKGLLL ANEVDGKRVR GLLENVERWG
160 170 180 190 200
APLAVTQAPP RALAEAFGTY FHRVLLDAPC SGEGMFRKDR EAARHWGPSA
210 220 230 240 250
PKRMAEVQKA LLAQASRLLG PGGVLVYSTC TFAPEENEGV VAHFLKAHPE
260 270 280 290 300
FRLEDARLHP LFAPGVPEWG EGNPELLKTA RLWPHRLEGE GHFLARFRKE
310 320 330 340 350
GGAWSTPRLE RPSPLSQEAL RAFRGFLEEA GLTLEGPVLD RAGHLYLLPE
360 370 380 390 400
GLPTLLGLKA PAPGLYLGKV QKGRFLPARA LALAFGATLP WPEGLPRLAL
410 420 430 440 450
TPEDPRALAF ATGEGVAWEG EDHPLALVVL KTAAGEFPLD FGKAKRGVLR

PVGVGL
Length:456
Mass (Da):49,740
Last modified:December 21, 2004 - v1
Checksum:iC440940306A6683E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA Translation: BAD71210.1
RefSeqiWP_011228642.1, NC_006461.1
YP_144653.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD71210; BAD71210; BAD71210
GeneIDi3168094
KEGGittj:TTHA1387
PATRICifig|300852.9.peg.1363

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP008226 Genomic DNA Translation: BAD71210.1
RefSeqiWP_011228642.1, NC_006461.1
YP_144653.1, NC_006461.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M6UX-ray1.40A/B1-456[»]
3M6VX-ray1.82A/B1-456[»]
3M6WX-ray1.30A1-456[»]
3M6XX-ray1.68A1-456[»]
ProteinModelPortaliQ5SII2
SMRiQ5SII2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1387

Chemistry databases

DrugBankiDB02899 N-Carboxymethionine

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71210; BAD71210; BAD71210
GeneIDi3168094
KEGGittj:TTHA1387
PATRICifig|300852.9.peg.1363

Phylogenomic databases

eggNOGiENOG4105CYJ Bacteria
COG0144 LUCA
HOGENOMiHOG000218116
OMAiDPLFHAG
PhylomeDBiQ5SII2

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-1392-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ5SII2

Family and domain databases

InterProiView protein in InterPro
IPR031341 Methyltr_RsmF_N
IPR001678 MeTrfase_RsmB/NOP2
IPR023267 RCMT
IPR031340 RsmF_methylt_CI
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF01189 Methyltr_RsmB-F, 1 hit
PF17125 Methyltr_RsmF_N, 1 hit
PF17126 RsmF_methylt_CI, 1 hit
PRINTSiPR02008 RCMTFAMILY
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51686 SAM_MT_RSMB_NOP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRSMF_THET8
AccessioniPrimary (citable) accession number: Q5SII2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: December 21, 2004
Last modified: February 28, 2018
This is version 74 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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