Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Elongation factor Tu-A

Gene

tufA

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
EF-Tu-GDP binds to the acceptor arm of tmRNA by interacting with its acceptor arm, suggesting that GTP hydrolysis by EF-Tu is essential for tmRNA function.1 Publication
Protects glycyl-tRNA(Gly) from hydrolysis by E.coli D-aminoacyl-tRNA deacylase (dtd) (PubMed:27224426).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 26GTP8
Nucleotide bindingi82 – 86GTP5
Nucleotide bindingi137 – 140GTP4

GO - Molecular functioni

Keywordsi

Molecular functionElongation factor, RNA-binding
Biological processProtein biosynthesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-1700-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor Tu-AUniRule annotation
Short name:
EF-Tu-AUniRule annotation
Gene namesi
Name:tufAUniRule annotation
Synonyms:tuf
Ordered Locus Names:TTHA1694
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000914232 – 406Elongation factor Tu-AAdd BLAST405

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei395PhosphothreonineBy similarity1

Post-translational modificationi

Phosphorylated on a threonine.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ5SHN6

PTM databases

iPTMnetiQ5SHN6

Interactioni

Subunit structurei

Monomer (By similarity). Binds to the 70S ribosome, contacts tmRNA during trans-translation (PubMed:12677067).UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi300852.TTHA1694

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ5SHN6
SMRiQ5SHN6
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ5SHN6

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini10 – 215tr-type GAdd BLAST206

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 26G1By similarity8
Regioni61 – 65G2By similarity5
Regioni82 – 85G3By similarity4
Regioni137 – 140G4By similarity4
Regioni175 – 177G5By similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CGV Bacteria
COG0050 LUCA
HOGENOMiHOG000229290
KOiK02358
OMAiAIRDMGM
PhylomeDBiQ5SHN6

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
HAMAPiMF_00118_B EF_Tu_B, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q5SHN6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAKGEFVRTK PHVNVGTIGH VDHGKTTLTA ALTYVAAAEN PNVEVKDYGD
60 70 80 90 100
IDKAPEERAR GITINTAHVE YETAKRHYSH VDCPGHADYI KNMITGAAQM
110 120 130 140 150
DGAILVVSAA DGPMPQTREH ILLARQVGVP YIVVFMNKVD MVDDPELLDL
160 170 180 190 200
VEMEVRDLLN QYEFPGDEVP VIRGSALLAL EQMHRNPKTR RGENEWVDKI
210 220 230 240 250
WELLDAIDEY IPTPVRDVDK PFLMPVEDVF TITGRGTVAT GRIERGKVKV
260 270 280 290 300
GDEVEIVGLA PETRRTVVTG VEMHRKTLQE GIAGDNVGVL LRGVSREEVE
310 320 330 340 350
RGQVLAKPGS ITPHTKFEAS VYVLKKEEGG RHTGFFSGYR PQFYFRTTDV
360 370 380 390 400
TGVVQLPPGV EMVMPGDNVT FTVELIKPVA LEEGLRFAIR EGGRTVGAGV

VTKILE
Length:406
Mass (Da):44,782
Last modified:January 23, 2007 - v3
Checksum:iBA597518358269E4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti380A → G in CAA29397 (PubMed:3317278).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05977 Genomic DNA Translation: CAA29397.1
X06657 Genomic DNA Translation: CAA29856.1
AP008226 Genomic DNA Translation: BAD71517.1
PIRiS00229
S17146
RefSeqiWP_011228847.1, NC_006461.1
YP_144960.1, NC_006461.1

Genome annotation databases

EnsemblBacteriaiBAD71517; BAD71517; BAD71517
GeneIDi3167925
KEGGittj:TTHA1694
PATRICifig|300852.9.peg.1664

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X05977 Genomic DNA Translation: CAA29397.1
X06657 Genomic DNA Translation: CAA29856.1
AP008226 Genomic DNA Translation: BAD71517.1
PIRiS00229
S17146
RefSeqiWP_011228847.1, NC_006461.1
YP_144960.1, NC_006461.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1HA3X-ray2.00A/B2-406[»]
1ZC8electron microscopy13.00Y2-406[»]
2C78X-ray1.40A2-406[»]
4V5GX-ray3.60AZ/CZ1-406[»]
4V5PX-ray3.10AZ/CZ2-406[»]
4V5QX-ray3.10AZ/CZ2-406[»]
4V5RX-ray3.10AZ/CZ2-406[»]
4V5SX-ray3.10AZ/CZ2-406[»]
4V8QX-ray3.10BZ2-406[»]
ProteinModelPortaliQ5SHN6
SMRiQ5SHN6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1694

PTM databases

iPTMnetiQ5SHN6

Proteomic databases

PRIDEiQ5SHN6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71517; BAD71517; BAD71517
GeneIDi3167925
KEGGittj:TTHA1694
PATRICifig|300852.9.peg.1664

Phylogenomic databases

eggNOGiENOG4105CGV Bacteria
COG0050 LUCA
HOGENOMiHOG000229290
KOiK02358
OMAiAIRDMGM
PhylomeDBiQ5SHN6

Enzyme and pathway databases

BioCyciTTHE300852:G1GKC-1700-MONOMER

Miscellaneous databases

EvolutionaryTraceiQ5SHN6

Family and domain databases

CDDicd03697 EFTU_II, 1 hit
HAMAPiMF_00118_B EF_Tu_B, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR033720 EFTU_2
IPR031157 G_TR_CS
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR009000 Transl_B-barrel_sf
IPR009001 Transl_elong_EF1A/Init_IF2_C
IPR004541 Transl_elong_EFTu/EF1A_bac/org
IPR004160 Transl_elong_EFTu/EF1A_C
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF03143 GTP_EFTU_D3, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF50465 SSF50465, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00485 EF-Tu, 1 hit
TIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS00301 G_TR_1, 1 hit
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEFTU1_THET8
AccessioniPrimary (citable) accession number: Q5SHN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 24, 2005
Last sequence update: January 23, 2007
Last modified: October 10, 2018
This is version 107 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again