Cnksr3

Functionⁱ

Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).By similarity

GO - Biological processⁱ

negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
positive regulation of sodium ion transport Source: UniProtKB

View the complete GO annotation on QuickGO ...

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Connector enhancer of kinase suppressor of ras 3 Short name: Connector enhancer of KSR 3 Alternative name(s): CNK homolog protein 3 Short name: CNK3 Maguin-like protein Parturition-related protein 4
Gene namesⁱ	Name:Cnksr3 Synonyms:Prp4
Organismⁱ	Rattus norvegicus (Rat)
Taxonomic identifierⁱ	10116 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus
Proteomesⁱ	UP000002494 Componentⁱ: Chromosome 1

Organism-specific databases

Rat genome database More...RGDⁱ	1307544 Cnksr3

RGDⁱ

1307544 Cnksr3

Keywords - Cellular componentⁱ

Cell membrane, Cytoplasm, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000311107	1 – 555	Connector enhancer of kinase suppressor of ras 3Add BLAST		555

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	381	PhosphoserineBy similarity		1
Modified residueⁱ	383	PhosphoserineCombined sources		1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

PaxDbⁱ	Q5SGD7
PRIDEⁱ	Q5SGD7

PTM databases

iPTMnetⁱ	Q5SGD7
PhosphoSitePlusⁱ	Q5SGD7

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSRNOG00000018052 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasⁱ	Q5SGD7 baseline and differential
Genevisibleⁱ	Q5SGD7 RN

Interactionⁱ

Subunit structureⁱ

Interacts with epithelial sodium channel ENaC. Interacts directly with SCNN1A (ENaC subunit alpha) and SCNN1B (ENaC subunit beta) C-terminal tails. Interacts with ENaC regulatory proteins NEDD4L, RAF1 and SGK1.By similarity

Protein-protein interaction databases

BioGridⁱ	258906, 1 interactor
STRINGⁱ	10116.ENSRNOP00000024418

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q5SGD7
SMRⁱ	Q5SGD7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	7 – 72	SAMPROSITE-ProRule annotationAdd BLAST	66
Domainⁱ	80 – 174	CRICPROSITE-ProRule annotationAdd BLAST	95
Domainⁱ	211 – 293	PDZPROSITE-ProRule annotationAdd BLAST	83
Domainⁱ	325 – 546	DUF1170Add BLAST	222

Domainⁱ

The PDZ domain is required for interaction with ENaC and SGK1, but not for interaction with NEDDL4 and RAF1.By similarity

Sequence similaritiesⁱ

Belongs to the CNKSR family.Curated

Phylogenomic databases

eggNOGⁱ	KOG1738 Eukaryota ENOG41104C7 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017199
HOGENOMⁱ	HOG000015752
HOVERGENⁱ	HBG051040
InParanoidⁱ	Q5SGD7
OMAⁱ	KCKQPLP
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G037J
PhylomeDBⁱ	Q5SGD7
TreeFamⁱ	TF326495

Family and domain databases

InterProⁱ	View protein in InterPro IPR010599 CNKSR2 IPR017874 CRIC_domain IPR001478 PDZ IPR036034 PDZ_sf IPR001660 SAM IPR013761 SAM/pointed_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF10534 CRIC_ras_sig, 1 hit PF06663 DUF1170, 1 hit PF00595 PDZ, 1 hit PF00536 SAM_1, 1 hit
SMARTⁱ	View protein in SMART SM00228 PDZ, 1 hit SM00454 SAM, 1 hit
SUPFAMⁱ	SSF47769 SSF47769, 1 hit SSF50156 SSF50156, 1 hit
PROSITEⁱ	View protein in PROSITE PS51290 CRIC, 1 hit PS50106 PDZ, 1 hit PS50105 SAM_DOMAIN, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q5SGD7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MEPVTKWSPK QVVDWTRGLD DCLQPYVHKF EREKIDGEQL LKISHQDLEE 
        60         70         80         90        100
LGVTRIGHQE LVLEAVDLLC ALNYGLETDN MKNLVLKLRA SSHNLQNYIS 
       110        120        130        140        150
SRRKSPAYDG NTSRKPPNEF LTSVVELIGA AKALLAWLDR APFTGITDLS 
       160        170        180        190        200
VTKNKIIQLC LDLTTAVQKD CLVAEMEDKV LNVVKVLNGI CDKMMHSTTD 
       210        220        230        240        250
PVMSQCACLE EVHLPNVRPG EGLGMYIKST YDGLHVITGT TENSPADRSQ 
       260        270        280        290        300
KIHAGDEVIQ VNRQTVVGWQ LKNLVKKLRE NPTGVVLLLK KRPTGSFSFT 
       310        320        330        340        350
PAPLKNLRWK PPLVQTSPPP TTTQSPESTM DASLKKEKPA ILDLYIPPPP 
       360        370        380        390        400
AVPYSPRDEN VSFSYRGHTK SKQPLPGRKG SESPNSFLDQ ESQRRRFTIT 
       410        420        430        440        450
DSDQLPGYSV GTNILPIKMR GKTPSYGKPR PLSMPADGNW MGIVDPFAKP 
       460        470        480        490        500
RGHGRKGEDA LCRYFSNERI TPIIEESASP VYRFSRPLTE RHLVRGADYI 
       510        520        530        540        550
RGSRCYINSD LHSSATIPFQ EEGPKKKSAS SSAKASSGEP SLLVSWLTRL 

KLLTH

Length:555

Mass (Da):61,851

Last modified:December 21, 2004 - v1

Checksum:ⁱ9427AEB3FF48626D

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A0G2JYE3	A0A0G2JYE3_RAT		Connector enhancer of kinase suppre... Connector enhancer of kinase suppressor of ras 3	Cnksr3	538	Annotation score:

Sequence cautionⁱ

The sequence AAR01114 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	358	D → Y in AAR01114 (Ref. 2) Curated		1
Sequence conflictⁱ	456	K → KG in AAR01114 (Ref. 2) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY328890 mRNA Translation: AAQ92305.1 AY333962 mRNA Translation: AAR01114.1 Different initiation.
NCBI Reference Sequences More...RefSeqⁱ	NP_001012061.1, NM_001012061.1
UniGeneⁱ	Rn.137681

Genome annotation databases

Ensemblⁱ	ENSRNOT00000024418; ENSRNOP00000024418; ENSRNOG00000018052
GeneIDⁱ	308113
KEGGⁱ	rno:308113
UCSC genome browser More...UCSCⁱ	RGD:1307544 rat

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q5SGD7	Connector enhancer of kinase suppressor of ras 3		RAT		538	UniRef100_Q5SGD7
UPI0003D0ACDD		RAT		475

Protein	Similar proteins		Species	Score	Length	Source
Q5SGD7	Connector enhancer of kinase suppressor of ras 3		HUMAN		555	UniRef90_Q6P9H4
Connector enhancer of kinase suppressor of ras 3		MOUSE		555
connector enhancer of kinase suppressor of ras 3		MESAU		555
Connector enhancer of kinase suppressor of ras 3		CRIGR		555
CNKSR family member 3		GORGO		555
+28

Protein	Similar proteins		Species	Score	Length	Source
Q5SGD7	Connector enhancer of kinase suppressor of ras 3		HUMAN		555	UniRef50_Q6P9H4
Connector enhancer of kinase suppressor of ras 3		MOUSE		555
Connector enhancer of kinase suppressor of ras 3		CRIGR		555
connector enhancer of kinase suppressor of ras 3		MESAU		555
Connector enhancer of kinase suppressor of ras 3		RAT		538
+357

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AY328890 mRNA Translation: AAQ92305.1 AY333962 mRNA Translation: AAR01114.1 Different initiation.
RefSeqⁱ	NP_001012061.1, NM_001012061.1
UniGeneⁱ	Rn.137681

3D structure databases

ProteinModelPortalⁱ	Q5SGD7
SMRⁱ	Q5SGD7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	258906, 1 interactor
STRINGⁱ	10116.ENSRNOP00000024418

PTM databases

iPTMnetⁱ	Q5SGD7
PhosphoSitePlusⁱ	Q5SGD7

Proteomic databases

PaxDbⁱ	Q5SGD7
PRIDEⁱ	Q5SGD7

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSRNOT00000024418; ENSRNOP00000024418; ENSRNOG00000018052
GeneIDⁱ	308113
KEGGⁱ	rno:308113
UCSCⁱ	RGD:1307544 rat

Organism-specific databases

CTDⁱ	154043
RGDⁱ	1307544 Cnksr3

Phylogenomic databases

eggNOGⁱ	KOG1738 Eukaryota ENOG41104C7 LUCA
GeneTreeⁱ	ENSGT00390000017199
HOGENOMⁱ	HOG000015752
HOVERGENⁱ	HBG051040
InParanoidⁱ	Q5SGD7
OMAⁱ	KCKQPLP
OrthoDBⁱ	EOG091G037J
PhylomeDBⁱ	Q5SGD7
TreeFamⁱ	TF326495

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q5SGD7

PROⁱ

PR:Q5SGD7

Gene expression databases

Bgeeⁱ	ENSRNOG00000018052 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasⁱ	Q5SGD7 baseline and differential
Genevisibleⁱ	Q5SGD7 RN

Family and domain databases

InterProⁱ	View protein in InterPro IPR010599 CNKSR2 IPR017874 CRIC_domain IPR001478 PDZ IPR036034 PDZ_sf IPR001660 SAM IPR013761 SAM/pointed_sf
Pfamⁱ	View protein in Pfam PF10534 CRIC_ras_sig, 1 hit PF06663 DUF1170, 1 hit PF00595 PDZ, 1 hit PF00536 SAM_1, 1 hit
SMARTⁱ	View protein in SMART SM00228 PDZ, 1 hit SM00454 SAM, 1 hit
SUPFAMⁱ	SSF47769 SSF47769, 1 hit SSF50156 SSF50156, 1 hit
PROSITEⁱ	View protein in PROSITE PS51290 CRIC, 1 hit PS50106 PDZ, 1 hit PS50105 SAM_DOMAIN, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CNKR3_RAT
Accessionⁱ	Q5SGD7Primary (citable) accession number: Q5SGD7 Secondary accession number(s): Q6VPP2
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
	Last sequence update:	December 21, 2004
	Last modified:	September 12, 2018
	This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - Q5SGD7 (CNKR3_RAT)

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Connector enhancer of kinase suppressor of ras 3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Other locations

Plasma Membrane

Other locations

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ