UniProtKB - Q5SGD7 (CNKR3_RAT)
Protein
Connector enhancer of kinase suppressor of ras 3
Gene
Cnksr3
Organism
Rattus norvegicus (Rat)
Status
Functioni
Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).By similarity
GO - Biological processi
- negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
- negative regulation of peptidyl-serine phosphorylation Source: UniProtKB
- positive regulation of sodium ion transmembrane transporter activity Source: RGD
- positive regulation of sodium ion transport Source: UniProtKB
Names & Taxonomyi
Protein namesi | Recommended name: Connector enhancer of kinase suppressor of ras 3Short name: Connector enhancer of KSR 3 Alternative name(s): CNK homolog protein 3 Short name: CNK3 Maguin-like protein Parturition-related protein 4 |
Gene namesi | Name:Cnksr3 Synonyms:Prp4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1307544, Cnksr3 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Plasma membrane
- Apical cell membrane By similarity; Peripheral membrane protein By similarity
Plasma Membrane
- apical plasma membrane Source: UniProtKB-SubCell
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell membrane, Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000311107 | 1 – 555 | Connector enhancer of kinase suppressor of ras 3Add BLAST | 555 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 381 | PhosphoserineBy similarity | 1 | |
Modified residuei | 383 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q5SGD7 |
PRIDEi | Q5SGD7 |
PTM databases
iPTMneti | Q5SGD7 |
PhosphoSitePlusi | Q5SGD7 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000018052, Expressed in cerebellum and 21 other tissues |
ExpressionAtlasi | Q5SGD7, baseline and differential |
Genevisiblei | Q5SGD7, RN |
Interactioni
Subunit structurei
Interacts with epithelial sodium channel ENaC.
Interacts directly with SCNN1A (ENaC subunit alpha) and SCNN1B (ENaC subunit beta) C-terminal tails.
Interacts with ENaC regulatory proteins NEDD4L, RAF1 and SGK1.
By similarityProtein-protein interaction databases
BioGRIDi | 258906, 1 interactor |
STRINGi | 10116.ENSRNOP00000024418 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 72 | SAMPROSITE-ProRule annotationAdd BLAST | 66 | |
Domaini | 80 – 174 | CRICPROSITE-ProRule annotationAdd BLAST | 95 | |
Domaini | 211 – 293 | PDZPROSITE-ProRule annotationAdd BLAST | 83 | |
Domaini | 325 – 546 | DUF1170Add BLAST | 222 |
Domaini
The PDZ domain is required for interaction with ENaC and SGK1, but not for interaction with NEDDL4 and RAF1.By similarity
Sequence similaritiesi
Belongs to the CNKSR family.Curated
Phylogenomic databases
eggNOGi | KOG1738, Eukaryota |
GeneTreei | ENSGT00940000154428 |
InParanoidi | Q5SGD7 |
OMAi | RCTIADY |
OrthoDBi | 1121556at2759 |
PhylomeDBi | Q5SGD7 |
TreeFami | TF326495 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR010599, CNKSR2 IPR017874, CRIC_domain IPR001478, PDZ IPR036034, PDZ_sf IPR001660, SAM IPR013761, SAM/pointed_sf |
Pfami | View protein in Pfam PF10534, CRIC_ras_sig, 1 hit PF06663, DUF1170, 1 hit PF00595, PDZ, 1 hit PF00536, SAM_1, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS51290, CRIC, 1 hit PS50106, PDZ, 1 hit PS50105, SAM_DOMAIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q5SGD7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEPVTKWSPK QVVDWTRGLD DCLQPYVHKF EREKIDGEQL LKISHQDLEE
60 70 80 90 100
LGVTRIGHQE LVLEAVDLLC ALNYGLETDN MKNLVLKLRA SSHNLQNYIS
110 120 130 140 150
SRRKSPAYDG NTSRKPPNEF LTSVVELIGA AKALLAWLDR APFTGITDLS
160 170 180 190 200
VTKNKIIQLC LDLTTAVQKD CLVAEMEDKV LNVVKVLNGI CDKMMHSTTD
210 220 230 240 250
PVMSQCACLE EVHLPNVRPG EGLGMYIKST YDGLHVITGT TENSPADRSQ
260 270 280 290 300
KIHAGDEVIQ VNRQTVVGWQ LKNLVKKLRE NPTGVVLLLK KRPTGSFSFT
310 320 330 340 350
PAPLKNLRWK PPLVQTSPPP TTTQSPESTM DASLKKEKPA ILDLYIPPPP
360 370 380 390 400
AVPYSPRDEN VSFSYRGHTK SKQPLPGRKG SESPNSFLDQ ESQRRRFTIT
410 420 430 440 450
DSDQLPGYSV GTNILPIKMR GKTPSYGKPR PLSMPADGNW MGIVDPFAKP
460 470 480 490 500
RGHGRKGEDA LCRYFSNERI TPIIEESASP VYRFSRPLTE RHLVRGADYI
510 520 530 540 550
RGSRCYINSD LHSSATIPFQ EEGPKKKSAS SSAKASSGEP SLLVSWLTRL
KLLTH
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JYE3 | A0A0G2JYE3_RAT | Connector enhancer of kinase suppre... | Cnksr3 | 538 | Annotation score: |
Sequence cautioni
The sequence AAR01114 differs from that shown. Reason: Erroneous initiation.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 358 | D → Y in AAR01114 (Ref. 2) Curated | 1 | |
Sequence conflicti | 456 | K → KG in AAR01114 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY328890 mRNA Translation: AAQ92305.1 AY333962 mRNA Translation: AAR01114.1 Different initiation. |
RefSeqi | NP_001012061.1, NM_001012061.1 |
Genome annotation databases
Ensembli | ENSRNOT00000024418; ENSRNOP00000024418; ENSRNOG00000018052 |
GeneIDi | 308113 |
KEGGi | rno:308113 |
UCSCi | RGD:1307544, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY328890 mRNA Translation: AAQ92305.1 AY333962 mRNA Translation: AAR01114.1 Different initiation. |
RefSeqi | NP_001012061.1, NM_001012061.1 |
3D structure databases
SMRi | Q5SGD7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 258906, 1 interactor |
STRINGi | 10116.ENSRNOP00000024418 |
PTM databases
iPTMneti | Q5SGD7 |
PhosphoSitePlusi | Q5SGD7 |
Proteomic databases
PaxDbi | Q5SGD7 |
PRIDEi | Q5SGD7 |
Genome annotation databases
Ensembli | ENSRNOT00000024418; ENSRNOP00000024418; ENSRNOG00000018052 |
GeneIDi | 308113 |
KEGGi | rno:308113 |
UCSCi | RGD:1307544, rat |
Organism-specific databases
CTDi | 154043 |
RGDi | 1307544, Cnksr3 |
Phylogenomic databases
eggNOGi | KOG1738, Eukaryota |
GeneTreei | ENSGT00940000154428 |
InParanoidi | Q5SGD7 |
OMAi | RCTIADY |
OrthoDBi | 1121556at2759 |
PhylomeDBi | Q5SGD7 |
TreeFami | TF326495 |
Miscellaneous databases
PROi | PR:Q5SGD7 |
Gene expression databases
Bgeei | ENSRNOG00000018052, Expressed in cerebellum and 21 other tissues |
ExpressionAtlasi | Q5SGD7, baseline and differential |
Genevisiblei | Q5SGD7, RN |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR010599, CNKSR2 IPR017874, CRIC_domain IPR001478, PDZ IPR036034, PDZ_sf IPR001660, SAM IPR013761, SAM/pointed_sf |
Pfami | View protein in Pfam PF10534, CRIC_ras_sig, 1 hit PF06663, DUF1170, 1 hit PF00595, PDZ, 1 hit PF00536, SAM_1, 1 hit |
SMARTi | View protein in SMART SM00228, PDZ, 1 hit SM00454, SAM, 1 hit |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS51290, CRIC, 1 hit PS50106, PDZ, 1 hit PS50105, SAM_DOMAIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CNKR3_RAT | |
Accessioni | Q5SGD7Primary (citable) accession number: Q5SGD7 Secondary accession number(s): Q6VPP2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 13, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | April 7, 2021 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families