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Protein

Connector enhancer of kinase suppressor of ras 3

Gene

Cnksr3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in transepithelial sodium transport. Regulates aldosterone-induced and epithelial sodium channel (ENaC)-mediated sodium transport through regulation of ENaC cell surface expression. Acts as a scaffold protein coordinating the assembly of an ENaC-regulatory complex (ERC).By similarity

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Connector enhancer of kinase suppressor of ras 3
Short name:
Connector enhancer of KSR 3
Alternative name(s):
CNK homolog protein 3
Short name:
CNK3
Maguin-like protein
Parturition-related protein 4
Gene namesi
Name:Cnksr3
Synonyms:Prp4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1307544 Cnksr3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003111071 – 555Connector enhancer of kinase suppressor of ras 3Add BLAST555

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei381PhosphoserineBy similarity1
Modified residuei383PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5SGD7
PRIDEiQ5SGD7

PTM databases

iPTMnetiQ5SGD7
PhosphoSitePlusiQ5SGD7

Expressioni

Gene expression databases

BgeeiENSRNOG00000018052 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiQ5SGD7 baseline and differential
GenevisibleiQ5SGD7 RN

Interactioni

Subunit structurei

Interacts with epithelial sodium channel ENaC. Interacts directly with SCNN1A (ENaC subunit alpha) and SCNN1B (ENaC subunit beta) C-terminal tails. Interacts with ENaC regulatory proteins NEDD4L, RAF1 and SGK1.By similarity

Protein-protein interaction databases

BioGridi258906, 1 interactor
STRINGi10116.ENSRNOP00000024418

Structurei

3D structure databases

ProteinModelPortaliQ5SGD7
SMRiQ5SGD7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 72SAMPROSITE-ProRule annotationAdd BLAST66
Domaini80 – 174CRICPROSITE-ProRule annotationAdd BLAST95
Domaini211 – 293PDZPROSITE-ProRule annotationAdd BLAST83
Domaini325 – 546DUF1170Add BLAST222

Domaini

The PDZ domain is required for interaction with ENaC and SGK1, but not for interaction with NEDDL4 and RAF1.By similarity

Sequence similaritiesi

Belongs to the CNKSR family.Curated

Phylogenomic databases

eggNOGiKOG1738 Eukaryota
ENOG41104C7 LUCA
GeneTreeiENSGT00390000017199
HOGENOMiHOG000015752
HOVERGENiHBG051040
InParanoidiQ5SGD7
OMAiKCKQPLP
OrthoDBiEOG091G037J
PhylomeDBiQ5SGD7
TreeFamiTF326495

Family and domain databases

InterProiView protein in InterPro
IPR010599 CNKSR2
IPR017874 CRIC_domain
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001660 SAM
IPR013761 SAM/pointed_sf
PfamiView protein in Pfam
PF10534 CRIC_ras_sig, 1 hit
PF06663 DUF1170, 1 hit
PF00595 PDZ, 1 hit
PF00536 SAM_1, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51290 CRIC, 1 hit
PS50106 PDZ, 1 hit
PS50105 SAM_DOMAIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q5SGD7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPVTKWSPK QVVDWTRGLD DCLQPYVHKF EREKIDGEQL LKISHQDLEE
60 70 80 90 100
LGVTRIGHQE LVLEAVDLLC ALNYGLETDN MKNLVLKLRA SSHNLQNYIS
110 120 130 140 150
SRRKSPAYDG NTSRKPPNEF LTSVVELIGA AKALLAWLDR APFTGITDLS
160 170 180 190 200
VTKNKIIQLC LDLTTAVQKD CLVAEMEDKV LNVVKVLNGI CDKMMHSTTD
210 220 230 240 250
PVMSQCACLE EVHLPNVRPG EGLGMYIKST YDGLHVITGT TENSPADRSQ
260 270 280 290 300
KIHAGDEVIQ VNRQTVVGWQ LKNLVKKLRE NPTGVVLLLK KRPTGSFSFT
310 320 330 340 350
PAPLKNLRWK PPLVQTSPPP TTTQSPESTM DASLKKEKPA ILDLYIPPPP
360 370 380 390 400
AVPYSPRDEN VSFSYRGHTK SKQPLPGRKG SESPNSFLDQ ESQRRRFTIT
410 420 430 440 450
DSDQLPGYSV GTNILPIKMR GKTPSYGKPR PLSMPADGNW MGIVDPFAKP
460 470 480 490 500
RGHGRKGEDA LCRYFSNERI TPIIEESASP VYRFSRPLTE RHLVRGADYI
510 520 530 540 550
RGSRCYINSD LHSSATIPFQ EEGPKKKSAS SSAKASSGEP SLLVSWLTRL

KLLTH
Length:555
Mass (Da):61,851
Last modified:December 21, 2004 - v1
Checksum:i9427AEB3FF48626D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JYE3A0A0G2JYE3_RAT
Connector enhancer of kinase suppre...
Cnksr3
538Annotation score:

Sequence cautioni

The sequence AAR01114 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti358D → Y in AAR01114 (Ref. 2) Curated1
Sequence conflicti456K → KG in AAR01114 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY328890 mRNA Translation: AAQ92305.1
AY333962 mRNA Translation: AAR01114.1 Different initiation.
RefSeqiNP_001012061.1, NM_001012061.1
UniGeneiRn.137681

Genome annotation databases

EnsembliENSRNOT00000024418; ENSRNOP00000024418; ENSRNOG00000018052
GeneIDi308113
KEGGirno:308113
UCSCiRGD:1307544 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY328890 mRNA Translation: AAQ92305.1
AY333962 mRNA Translation: AAR01114.1 Different initiation.
RefSeqiNP_001012061.1, NM_001012061.1
UniGeneiRn.137681

3D structure databases

ProteinModelPortaliQ5SGD7
SMRiQ5SGD7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi258906, 1 interactor
STRINGi10116.ENSRNOP00000024418

PTM databases

iPTMnetiQ5SGD7
PhosphoSitePlusiQ5SGD7

Proteomic databases

PaxDbiQ5SGD7
PRIDEiQ5SGD7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024418; ENSRNOP00000024418; ENSRNOG00000018052
GeneIDi308113
KEGGirno:308113
UCSCiRGD:1307544 rat

Organism-specific databases

CTDi154043
RGDi1307544 Cnksr3

Phylogenomic databases

eggNOGiKOG1738 Eukaryota
ENOG41104C7 LUCA
GeneTreeiENSGT00390000017199
HOGENOMiHOG000015752
HOVERGENiHBG051040
InParanoidiQ5SGD7
OMAiKCKQPLP
OrthoDBiEOG091G037J
PhylomeDBiQ5SGD7
TreeFamiTF326495

Miscellaneous databases

PROiPR:Q5SGD7

Gene expression databases

BgeeiENSRNOG00000018052 Expressed in 10 organ(s), highest expression level in adult mammalian kidney
ExpressionAtlasiQ5SGD7 baseline and differential
GenevisibleiQ5SGD7 RN

Family and domain databases

InterProiView protein in InterPro
IPR010599 CNKSR2
IPR017874 CRIC_domain
IPR001478 PDZ
IPR036034 PDZ_sf
IPR001660 SAM
IPR013761 SAM/pointed_sf
PfamiView protein in Pfam
PF10534 CRIC_ras_sig, 1 hit
PF06663 DUF1170, 1 hit
PF00595 PDZ, 1 hit
PF00536 SAM_1, 1 hit
SMARTiView protein in SMART
SM00228 PDZ, 1 hit
SM00454 SAM, 1 hit
SUPFAMiSSF47769 SSF47769, 1 hit
SSF50156 SSF50156, 1 hit
PROSITEiView protein in PROSITE
PS51290 CRIC, 1 hit
PS50106 PDZ, 1 hit
PS50105 SAM_DOMAIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCNKR3_RAT
AccessioniPrimary (citable) accession number: Q5SGD7
Secondary accession number(s): Q6VPP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: December 21, 2004
Last modified: September 12, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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