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Protein

Insulin-like growth factor 2 mRNA-binding protein 2

Gene

Igf2bp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

RNA-binding factor that recruits target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation (By similarity). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. Binds to beta-actin/ACTB and MYC transcripts (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA transport, Translation regulation, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor 2 mRNA-binding protein 2
Short name:
IGF2 mRNA-binding protein 2
Short name:
IMP-2
Alternative name(s):
IGF-II mRNA-binding protein 2
VICKZ family member 2
Gene namesi
Name:Igf2bp2
Synonyms:Imp2, Vickz2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1890358 Igf2bp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002444971 – 592Insulin-like growth factor 2 mRNA-binding protein 2Add BLAST592

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei11PhosphoserineBy similarity1
Modified residuei162PhosphoserineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei543PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ5SF07
PeptideAtlasiQ5SF07
PRIDEiQ5SF07

2D gel databases

REPRODUCTION-2DPAGEiQ5SF07

PTM databases

iPTMnetiQ5SF07
PhosphoSitePlusiQ5SF07

Expressioni

Tissue specificityi

Expressed in oocytes, granulosa cells of small and growing follicles and Leydig cells of the testis (at protein level). Expressed in testis and ovary.1 Publication

Developmental stagei

Expressed in zygotes and blastocysts (at protein level). Expressed in gonads at 12.5, 14.5 and 16.5 dpc (at protein level). Expressed during fetal development at 12.5, 14.5 and 17.5 dpc and declining towards birth.2 Publications

Gene expression databases

BgeeiENSMUSG00000033581 Expressed in 216 organ(s), highest expression level in primary oocyte
ExpressionAtlasiQ5SF07 baseline and differential
GenevisibleiQ5SF07 MM

Interactioni

Subunit structurei

Can form homooligomers and heterooligomers with IGF2BP1 and IGF2BP3 in an RNA-dependent manner. Interacts with HNRPD and IGF2BP1. Interacts with ELAVL1, DHX9 and HNRNPU (By similarity).By similarity

Protein-protein interaction databases

BioGridi235510, 3 interactors
IntActiQ5SF07, 1 interactor
STRINGi10090.ENSMUSP00000097629

Structurei

3D structure databases

ProteinModelPortaliQ5SF07
SMRiQ5SF07
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 76RRM 1PROSITE-ProRule annotationAdd BLAST74
Domaini82 – 157RRM 2PROSITE-ProRule annotationAdd BLAST76
Domaini186 – 251KH 1PROSITE-ProRule annotationAdd BLAST66
Domaini267 – 334KH 2PROSITE-ProRule annotationAdd BLAST68
Domaini420 – 485KH 3PROSITE-ProRule annotationAdd BLAST66
Domaini502 – 568KH 4PROSITE-ProRule annotationAdd BLAST67

Domaini

Domain KH3 and KH4 are the major RNA-binding modules, although KH1 and KH2 may also contribute. The contribution to RNA-binding of individual KH domains may be target-specific. KH1 and KH2, and possibly KH3 and KH4, promote the formation of higher ordered protein-RNA complexes, which may be essential for IGF2BP1 cytoplasmic retention. KH domains are required for RNA-dependent homo- and heterooligomerization and for localization to stress granules (By similarity).By similarity

Sequence similaritiesi

Belongs to the RRM IMP/VICKZ family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2193 Eukaryota
ENOG410ZKB4 LUCA
GeneTreeiENSGT00530000063171
HOGENOMiHOG000000675
HOVERGENiHBG052725
InParanoidiQ5SF07
KOiK17392
OrthoDBiEOG091G17T1
PhylomeDBiQ5SF07
TreeFamiTF320229

Family and domain databases

CDDicd12626 RRM1_IGF2BP2, 1 hit
Gene3Di3.30.1370.10, 2 hits
3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR028743 IGF2BP2
IPR034843 IGF2BP2_RRM1
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR10288:SF93 PTHR10288:SF93, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 4 hits
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00322 KH, 4 hits
SM00360 RRM, 2 hits
SUPFAMiSSF54791 SSF54791, 4 hits
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 4 hits
PS50102 RRM, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5SF07-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MMNKLYIGNL SPAVTADDLR QLFGDRKLPL AGQVLLKSGY AFVDYPDQNW
60 70 80 90 100
AIRAIETLSG KVELHGKIME VDYSVSKKLR SRRIQIRNIP PHLQWEVLDG
110 120 130 140 150
LLAEYGTVEN VEQVNTDTET AVVNVTYMTR EEAKLAIEKL SGHQFEDYSF
160 170 180 190 200
KISYIPDEEV SSPSPPHRAR EQGHGPGSSS QARQIDFPLR ILVPTQFVGA
210 220 230 240 250
IIGKEGLTIK NITKQTQSRV DIHRKENSGA AEKPVTIHAT PEGTSEACRM
260 270 280 290 300
ILEIMQKEAD ETKLAEEVPL KILAHNGFVG RLIGKEGRNL KKIEHETGTK
310 320 330 340 350
ITISSLQDLS IYNPERTITV RGTIEACANA EIEIMKKLRE AFENDMLAVN
360 370 380 390 400
QQANLIPGLN LSALGIFSTG LSVLPPPAGP RGVPPSPPYH PFATHSGYFS
410 420 430 440 450
SLYPHHHFGP FPHHHSYPEQ ETVSLFIPTQ AVGAIIGKKG AHIKQLARFA
460 470 480 490 500
GASIKIAPAE GPDVSERMVI ITGPPEAQFK AQGRIFGKLK EENFFNPKEE
510 520 530 540 550
VKLEAHIRVP SSTAGRVIGK GGKTVNELQN LTSAEVIVPR DQTPDENEEV
560 570 580 590
IVRIIGHFFA SQTAQRKIRE IVQQVKQQEQ RYPQGVAPQR SK
Length:592
Mass (Da):65,584
Last modified:December 21, 2004 - v1
Checksum:i9D0E420758F5D1BE
GO
Isoform 2 (identifier: Q5SF07-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: MMNKLYIGNL...EVDYSVSKKL → MHPSQWPRYGAAGKPFS

Show »
Length:530
Mass (Da):58,659
Checksum:iFB5F89AC81D0835D
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A6X8Z3A6X8Z3_MOUSE
Insulin-like growth factor 2 mRNA-b...
Igf2bp2
524Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti568I → V in BAE41861 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0195761 – 79MMNKL…VSKKL → MHPSQWPRYGAAGKPFS in isoform 2. 1 PublicationAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY531659 mRNA Translation: AAT01428.1
AK170531 mRNA Translation: BAE41861.1
CT009567 Genomic DNA No translation available.
CCDSiCCDS49797.1 [Q5SF07-1]
RefSeqiNP_898850.2, NM_183029.2 [Q5SF07-1]
UniGeneiMm.489442

Genome annotation databases

EnsembliENSMUST00000100052; ENSMUSP00000097629; ENSMUSG00000033581 [Q5SF07-1]
GeneIDi319765
KEGGimmu:319765
UCSCiuc007yrz.1 mouse [Q5SF07-2]
uc007ysa.1 mouse [Q5SF07-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY531659 mRNA Translation: AAT01428.1
AK170531 mRNA Translation: BAE41861.1
CT009567 Genomic DNA No translation available.
CCDSiCCDS49797.1 [Q5SF07-1]
RefSeqiNP_898850.2, NM_183029.2 [Q5SF07-1]
UniGeneiMm.489442

3D structure databases

ProteinModelPortaliQ5SF07
SMRiQ5SF07
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi235510, 3 interactors
IntActiQ5SF07, 1 interactor
STRINGi10090.ENSMUSP00000097629

PTM databases

iPTMnetiQ5SF07
PhosphoSitePlusiQ5SF07

2D gel databases

REPRODUCTION-2DPAGEiQ5SF07

Proteomic databases

PaxDbiQ5SF07
PeptideAtlasiQ5SF07
PRIDEiQ5SF07

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000100052; ENSMUSP00000097629; ENSMUSG00000033581 [Q5SF07-1]
GeneIDi319765
KEGGimmu:319765
UCSCiuc007yrz.1 mouse [Q5SF07-2]
uc007ysa.1 mouse [Q5SF07-1]

Organism-specific databases

CTDi10644
MGIiMGI:1890358 Igf2bp2

Phylogenomic databases

eggNOGiKOG2193 Eukaryota
ENOG410ZKB4 LUCA
GeneTreeiENSGT00530000063171
HOGENOMiHOG000000675
HOVERGENiHBG052725
InParanoidiQ5SF07
KOiK17392
OrthoDBiEOG091G17T1
PhylomeDBiQ5SF07
TreeFamiTF320229

Miscellaneous databases

ChiTaRSiIgf2bp2 mouse
PROiPR:Q5SF07
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033581 Expressed in 216 organ(s), highest expression level in primary oocyte
ExpressionAtlasiQ5SF07 baseline and differential
GenevisibleiQ5SF07 MM

Family and domain databases

CDDicd12626 RRM1_IGF2BP2, 1 hit
Gene3Di3.30.1370.10, 2 hits
3.30.70.330, 2 hits
InterProiView protein in InterPro
IPR028743 IGF2BP2
IPR034843 IGF2BP2_RRM1
IPR004087 KH_dom
IPR004088 KH_dom_type_1
IPR036612 KH_dom_type_1_sf
IPR012677 Nucleotide-bd_a/b_plait_sf
IPR035979 RBD_domain_sf
IPR000504 RRM_dom
PANTHERiPTHR10288:SF93 PTHR10288:SF93, 1 hit
PfamiView protein in Pfam
PF00013 KH_1, 4 hits
PF00076 RRM_1, 2 hits
SMARTiView protein in SMART
SM00322 KH, 4 hits
SM00360 RRM, 2 hits
SUPFAMiSSF54791 SSF54791, 4 hits
SSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50084 KH_TYPE_1, 4 hits
PS50102 RRM, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiIF2B2_MOUSE
AccessioniPrimary (citable) accession number: Q5SF07
Secondary accession number(s): A6X8Z4, Q3TCU4, Q7TQF9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 27, 2006
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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