Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

D-ribitol-5-phosphate cytidylyltransferase

Gene

Ispd

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cytidylyltransferase required for protein O-linked mannosylation (By similarity). Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate. CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (By similarity). Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively (By similarity). Not Involved in dolichol production (By similarity).By similarity

Catalytic activityi

CTP + D-ribitol 5-phosphate = diphosphate + CDP-ribitol.By similarity
CTP + D-ribose 5-phosphate = diphosphate + CDP-ribose.By similarity
CTP + D-ribulose 5-phosphate = diphosphate + CDP-ribulose.By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei57Transition state stabilizerBy similarity1
Sitei64Transition state stabilizerBy similarity1
Sitei203Positions substrate for the nucleophilic attackBy similarity1
Sitei261Positions substrate for the nucleophilic attackBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase

Enzyme and pathway databases

UniPathwayi
UPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
D-ribitol-5-phosphate cytidylyltransferaseBy similarity (EC:2.7.7.40By similarity)
Alternative name(s):
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase-like proteinBy similarity
Isoprenoid synthase domain-containing proteinImported
Notch1-induced protein1 Publication
Gene namesi
Name:IspdImported
Synonyms:Nip1 Publication
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi1359368 Ispd

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003436991 – 447D-ribitol-5-phosphate cytidylyltransferaseAdd BLAST447

Proteomic databases

PaxDbiQ5S6T3
PRIDEiQ5S6T3

Expressioni

Gene expression databases

BgeeiENSRNOG00000006199 Expressed in 10 organ(s), highest expression level in heart
ExpressionAtlasiQ5S6T3 baseline and differential
GenevisibleiQ5S6T3 RN

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008312

Structurei

3D structure databases

ProteinModelPortaliQ5S6T3
SMRiQ5S6T3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IIH2 Eukaryota
COG1211 LUCA
GeneTreeiENSGT00390000006412
HOVERGENiHBG057782
InParanoidiQ5S6T3
KOiK21031
OMAiIILEQCY
OrthoDBiEOG091G0JSI
PhylomeDBiQ5S6T3
TreeFamiTF328415

Family and domain databases

CDDicd02516 CDP-ME_synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR034683 IspD/TarI
IPR018294 ISPD_synthase_CS
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01128 IspD, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS01295 ISPD, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q5S6T3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MESGPCSRPA EPRHCVSGRA GAGLAFPAFP LSAAGAEPGS RIGTVAAVLP
60 70 80 90 100
AGGCGERMGV RTPKQFCRVL ERPLISYTLQ AMERVCWIKD IVVTVTGENM
110 120 130 140 150
EAMRSIIQRY GHKRISLAEA GATRHRSIFN GLKALAEDQP GCELTRPEVV
160 170 180 190 200
IIHDAVRPFV EEDILLRVVL AAKEHGAAGA IRPLVSTVVS PSADGHLDHS
210 220 230 240 250
LDRAKHRASE MPQAFHFDVI YEAYQKCSDF DLEFGTECLQ LALKYCHRKA
260 270 280 290 300
KLVEGTPDLW KVTYKQDLCA AEAMIKEKIS QEICVVVNTK DEESVGHLLE
310 320 330 340 350
EVLRNELNCI KVTSTVLDRT SGDIENFIDQ CYSFICVNVV SSESRETRKL
360 370 380 390 400
LSILEESSLP LLYPVVVVLV HCFDFTVVPL AQKMENLVWI RELAKEAKGR
410 420 430 440
NVLLSGVLLN HSQDEQKLQE SLVQSAAIIA ALVKERNSAL VGQLLVA
Length:447
Mass (Da):49,192
Last modified:December 21, 2004 - v1
Checksum:i63FC38515B4F8FDE
GO
Isoform 2 (identifier: Q5S6T3-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-99: Missing.

Show »
Length:348
Mass (Da):38,840
Checksum:i4575730394E404E2
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2K627A0A0G2K627_RAT
D-ribitol-5-phosphate cytidylyltran...
Ispd
413Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0346741 – 99Missing in isoform 2. 1 PublicationAdd BLAST99

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY769991 mRNA Translation: AAV53695.1
AY769992 mRNA Translation: AAV53696.1
RefSeqiNP_001008387.1, NM_001008386.1 [Q5S6T3-1]
UniGeneiRn.119606

Genome annotation databases

EnsembliENSRNOT00000008312; ENSRNOP00000008312; ENSRNOG00000006199 [Q5S6T3-1]
GeneIDi493574
KEGGirno:493574
UCSCiRGD:1359368 rat [Q5S6T3-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY769991 mRNA Translation: AAV53695.1
AY769992 mRNA Translation: AAV53696.1
RefSeqiNP_001008387.1, NM_001008386.1 [Q5S6T3-1]
UniGeneiRn.119606

3D structure databases

ProteinModelPortaliQ5S6T3
SMRiQ5S6T3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000008312

Proteomic databases

PaxDbiQ5S6T3
PRIDEiQ5S6T3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008312; ENSRNOP00000008312; ENSRNOG00000006199 [Q5S6T3-1]
GeneIDi493574
KEGGirno:493574
UCSCiRGD:1359368 rat [Q5S6T3-1]

Organism-specific databases

CTDi729920
RGDi1359368 Ispd

Phylogenomic databases

eggNOGiENOG410IIH2 Eukaryota
COG1211 LUCA
GeneTreeiENSGT00390000006412
HOVERGENiHBG057782
InParanoidiQ5S6T3
KOiK21031
OMAiIILEQCY
OrthoDBiEOG091G0JSI
PhylomeDBiQ5S6T3
TreeFamiTF328415

Enzyme and pathway databases

UniPathwayi
UPA00378

Miscellaneous databases

PROiPR:Q5S6T3

Gene expression databases

BgeeiENSRNOG00000006199 Expressed in 10 organ(s), highest expression level in heart
ExpressionAtlasiQ5S6T3 baseline and differential
GenevisibleiQ5S6T3 RN

Family and domain databases

CDDicd02516 CDP-ME_synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR034683 IspD/TarI
IPR018294 ISPD_synthase_CS
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01128 IspD, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS01295 ISPD, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISPD_RAT
AccessioniPrimary (citable) accession number: Q5S6T3
Secondary accession number(s): Q5S6T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2008
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again