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Protein

DNA polymerase lambda

Gene

Poll

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

DNA polymerase that functions in several pathways of DNA repair. Involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Also contributes to DNA double-strand break repair by non-homologous end joining and homologous recombination. Has both template-dependent and template-independent (terminal transferase) DNA polymerase activities. Has also a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).By similarity

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei310Schiff-base intermediate with DNABy similarity1
Binding sitei384dCTPBy similarity1
Metal bindingi425ManganeseBy similarity1
Metal bindingi427ManganeseBy similarity1
Metal bindingi488ManganeseBy similarity1
Binding sitei511dCTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi415 – 418dCTP bindingBy similarity4
Nucleotide bindingi424 – 427dCTP bindingBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Lyase, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication, DNA synthesis
LigandManganese, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-5693571 Nonhomologous End-Joining (NHEJ)

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase lambdaCurated (EC:2.7.7.7By similarity, EC:4.2.99.-By similarity)
Short name:
Pol LambdaCurated
Gene namesi
Name:PollImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi1308053 Poll

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187861 – 573DNA polymerase lambdaAdd BLAST573

Proteomic databases

PaxDbiQ5RKI3
PRIDEiQ5RKI3

PTM databases

iPTMnetiQ5RKI3
PhosphoSitePlusiQ5RKI3

Expressioni

Gene expression databases

BgeeiENSRNOG00000016748 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiQ5RKI3 RN

Interactioni

Subunit structurei

Binds PCNA.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023137

Structurei

3D structure databases

ProteinModelPortaliQ5RKI3
SMRiQ5RKI3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini35 – 131BRCTPROSITE-ProRule annotationAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni263 – 277DNA bindingBy similarityAdd BLAST15
Regioni343 – 346DNA bindingBy similarity4
Regioni418 – 427Involved in primer bindingBy similarity10
Regioni464 – 503DNA bindingBy similarityAdd BLAST40

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated

Phylogenomic databases

eggNOGiKOG2534 Eukaryota
COG1796 LUCA
GeneTreeiENSGT00530000063002
HOGENOMiHOG000007787
HOVERGENiHBG002788
InParanoidiQ5RKI3
KOiK03512
OMAiKYLGVCQ
OrthoDBiEOG091G03V5
PhylomeDBiQ5RKI3
TreeFamiTF103011

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR018944 DNA_pol_lambd_fingers_domain
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR002008 DNA_pol_X_beta-like
IPR029398 PolB_thumb
PfamiView protein in Pfam
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF10391 DNA_pol_lambd_f, 1 hit
PF14716 HHH_8, 1 hit
PRINTSiPR00869 DNAPOLX
PR00870 DNAPOLXBETA
SMARTiView protein in SMART
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS00522 DNA_POLYMERASE_X, 1 hit

Sequencei

Sequence statusi: Complete.

Q5RKI3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDPQGILKAF PKRKKIHADP SSNALAKIPK REAGDARGWL SSLRAHIMPT
60 70 80 90 100
GIGRARAELF EKQIIQHGGQ VCSAQAPGVT HIVVDEGMDY ERALRLLRLP
110 120 130 140 150
QLPPGAQLVK SAWLSLCLQE KKLTDTDGFS LSSPKRSLNE PQPSKSGQDA
160 170 180 190 200
SAPGTQGVLP RTTLSLSPPC TRAVSPPPKA EKPPKTQTQL SSEDEASDGE
210 220 230 240 250
GPQVSSADLQ ALISGHYPTP PGEDGGPDPA PEALGKWVCA QPSSQKATNY
260 270 280 290 300
NLHITEKLEV LAKAYNVQGD KWRALGYAKA INALKSFHKP VSSYQEACSI
310 320 330 340 350
PGVGRRMAEK VMEILESGHL RKLDHISDSV PVLELFSNIW GAGTKTAQMW
360 370 380 390 400
YHQGFRSLED IRGLASLTAQ QAIGLKHYDD FLDRMPREEA AEIEQMVRVS
410 420 430 440 450
AQAFNPGLLC VACGSFRRGK VTCGDVDVLI THPDGRSHQG IFSPLLDSLR
460 470 480 490 500
QQGFLTDDLV SQEENGQQQK YLGVCRLPGA GQRHRRLDII VVPYSEFACA
510 520 530 540 550
LLYFTGSAHF NRSMRALAKT KGMSLSEHAL SAAVVRNSQG VKVGAGQVLP
560 570
TPTEKDVFKL LGLPYREPAE RDW
Length:573
Mass (Da):62,401
Last modified:December 21, 2004 - v1
Checksum:i5978076D6CC463B0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085841 mRNA Translation: AAH85841.1
RefSeqiNP_001014190.1, NM_001014168.1
UniGeneiRn.11799

Genome annotation databases

EnsembliENSRNOT00000023137; ENSRNOP00000023137; ENSRNOG00000016748
GeneIDi361767
KEGGirno:361767

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC085841 mRNA Translation: AAH85841.1
RefSeqiNP_001014190.1, NM_001014168.1
UniGeneiRn.11799

3D structure databases

ProteinModelPortaliQ5RKI3
SMRiQ5RKI3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023137

PTM databases

iPTMnetiQ5RKI3
PhosphoSitePlusiQ5RKI3

Proteomic databases

PaxDbiQ5RKI3
PRIDEiQ5RKI3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023137; ENSRNOP00000023137; ENSRNOG00000016748
GeneIDi361767
KEGGirno:361767

Organism-specific databases

CTDi27343
RGDi1308053 Poll

Phylogenomic databases

eggNOGiKOG2534 Eukaryota
COG1796 LUCA
GeneTreeiENSGT00530000063002
HOGENOMiHOG000007787
HOVERGENiHBG002788
InParanoidiQ5RKI3
KOiK03512
OMAiKYLGVCQ
OrthoDBiEOG091G03V5
PhylomeDBiQ5RKI3
TreeFamiTF103011

Enzyme and pathway databases

ReactomeiR-RNO-5693571 Nonhomologous End-Joining (NHEJ)

Miscellaneous databases

PROiPR:Q5RKI3

Gene expression databases

BgeeiENSRNOG00000016748 Expressed in 10 organ(s), highest expression level in skeletal muscle tissue
GenevisibleiQ5RKI3 RN

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR018944 DNA_pol_lambd_fingers_domain
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR002008 DNA_pol_X_beta-like
IPR029398 PolB_thumb
PfamiView protein in Pfam
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF10391 DNA_pol_lambd_f, 1 hit
PF14716 HHH_8, 1 hit
PRINTSiPR00869 DNAPOLX
PR00870 DNAPOLXBETA
SMARTiView protein in SMART
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS00522 DNA_POLYMERASE_X, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPOLL_RAT
AccessioniPrimary (citable) accession number: Q5RKI3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: December 21, 2004
Last modified: November 7, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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