UniProtKB - Q5RKI1 (IF4A2_RAT)
Eukaryotic initiation factor 4A-II
Eif4a2
Functioni
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon (By similarity).
By similarityCatalytic activityi
- EC:3.6.4.13
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 77 – 84 | ATPPROSITE-ProRule annotation | 8 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: RGD
- RNA helicase activity Source: UniProtKB-EC
- translation initiation factor activity Source: GO_Central
GO - Biological processi
- cellular response to leukemia inhibitory factor Source: RGD
- cytoplasmic translational initiation Source: GO_Central
- negative regulation of RNA-directed 5'-3' RNA polymerase activity Source: RGD
Keywordsi
Molecular function | Helicase, Hydrolase, Initiation factor, RNA-binding |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-RNO-1169408, ISG15 antiviral mechanism R-RNO-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-RNO-429947, Deadenylation of mRNA R-RNO-72649, Translation initiation complex formation R-RNO-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-RNO-72702, Ribosomal scanning and start codon recognition R-RNO-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Names & Taxonomyi
Protein namesi | Recommended name: Eukaryotic initiation factor 4A-II (EC:3.6.4.13)Short name: eIF-4A-II Short name: eIF4A-II Alternative name(s): ATP-dependent RNA helicase eIF4A-2 |
Gene namesi | Name:Eif4a2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 1309225, Eif4a2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000252335 | 1 – 407 | Eukaryotic initiation factor 4A-IIAdd BLAST | 407 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 159 | PhosphothreonineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q5RKI1 |
PaxDbi | Q5RKI1 |
PeptideAtlasi | Q5RKI1 |
2D gel databases
World-2DPAGEi | 0004:Q5RKI1 |
PTM databases
iPTMneti | Q5RKI1 |
PhosphoSitePlusi | Q5RKI1 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000001815, Expressed in Ammon's horn and 21 other tissues |
ExpressionAtlasi | Q5RKI1, baseline and differential |
Genevisiblei | Q5RKI1, RN |
Interactioni
Subunit structurei
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIFFG3 (By similarity).
Interacts with EIF4E. May interact with NOM1 (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 257691, 3 interactors |
IntActi | Q5RKI1, 2 interactors |
MINTi | Q5RKI1 |
STRINGi | 10116.ENSRNOP00000002484 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 64 – 235 | Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST | 172 | |
Domaini | 246 – 407 | Helicase C-terminalPROSITE-ProRule annotationAdd BLAST | 162 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 22 | DisorderedSequence analysisAdd BLAST | 22 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 33 – 61 | Q motifAdd BLAST | 29 | |
Motifi | 183 – 186 | DEAD box | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0327, Eukaryota |
GeneTreei | ENSGT00940000153783 |
HOGENOMi | CLU_003041_1_0_1 |
InParanoidi | Q5RKI1 |
OMAi | RNPIRIL |
OrthoDBi | 726081at2759 |
PhylomeDBi | Q5RKI1 |
Family and domain databases
CDDi | cd18046, DEADc_EIF4AII_EIF4AI_DDX2, 1 hit |
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR044728, EIF4A_DEADc IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MSGGSADYNR EHGGPEGMDP DGVIESNWNE IVDNFDDMNL KESLLRGIYA
60 70 80 90 100
YGFEKPSAIQ QRAIIPCIKG YDVIAQAQSG TGKTATFAIS ILQQLEIEFK
110 120 130 140 150
ETQALVLAPT RELAQQIQKV ILALGDYMGA TCHACIGGTN VRNEMQKLQA
160 170 180 190 200
EAPHIVVGTP GRVFDMLNRR YLSPKWIKMF VLDEADEMLS RGFKDQIYEI
210 220 230 240 250
FQKLNTSIQV VLLSATMPTD VLEVTKKFMR DPIRILVKKE ELTLEGIKQF
260 270 280 290 300
YINVEREEWK LDTLCDLYET LTITQAVIFL NTRRKVDWLT EKMHARDFTV
310 320 330 340 350
SALHGDMDQK ERDVIMREFR SGSSRVLITT DLLARGIDVQ QVSLVINYDL
360 370 380 390 400
PTNRENYIHR IGRGGRFGRK GVAINFVTEE DKRILRDIET FYNTTVEEMP
MNVADLI
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2K8B7 | A0A0G2K8B7_RAT | RNA helicase | Eif4a2 | 362 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC085859 mRNA Translation: AAH85859.1 |
RefSeqi | NP_001008336.1, NM_001008335.1 |
Genome annotation databases
Ensembli | ENSRNOT00000002484; ENSRNOP00000002484; ENSRNOG00000001815 |
GeneIDi | 303831 |
KEGGi | rno:303831 |
UCSCi | RGD:1309225, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC085859 mRNA Translation: AAH85859.1 |
RefSeqi | NP_001008336.1, NM_001008335.1 |
3D structure databases
SMRi | Q5RKI1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 257691, 3 interactors |
IntActi | Q5RKI1, 2 interactors |
MINTi | Q5RKI1 |
STRINGi | 10116.ENSRNOP00000002484 |
PTM databases
iPTMneti | Q5RKI1 |
PhosphoSitePlusi | Q5RKI1 |
2D gel databases
World-2DPAGEi | 0004:Q5RKI1 |
Proteomic databases
jPOSTi | Q5RKI1 |
PaxDbi | Q5RKI1 |
PeptideAtlasi | Q5RKI1 |
Genome annotation databases
Ensembli | ENSRNOT00000002484; ENSRNOP00000002484; ENSRNOG00000001815 |
GeneIDi | 303831 |
KEGGi | rno:303831 |
UCSCi | RGD:1309225, rat |
Organism-specific databases
CTDi | 1974 |
RGDi | 1309225, Eif4a2 |
Phylogenomic databases
eggNOGi | KOG0327, Eukaryota |
GeneTreei | ENSGT00940000153783 |
HOGENOMi | CLU_003041_1_0_1 |
InParanoidi | Q5RKI1 |
OMAi | RNPIRIL |
OrthoDBi | 726081at2759 |
PhylomeDBi | Q5RKI1 |
Enzyme and pathway databases
Reactomei | R-RNO-1169408, ISG15 antiviral mechanism R-RNO-156827, L13a-mediated translational silencing of Ceruloplasmin expression R-RNO-429947, Deadenylation of mRNA R-RNO-72649, Translation initiation complex formation R-RNO-72662, Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S R-RNO-72702, Ribosomal scanning and start codon recognition R-RNO-72706, GTP hydrolysis and joining of the 60S ribosomal subunit |
Miscellaneous databases
PROi | PR:Q5RKI1 |
Gene expression databases
Bgeei | ENSRNOG00000001815, Expressed in Ammon's horn and 21 other tissues |
ExpressionAtlasi | Q5RKI1, baseline and differential |
Genevisiblei | Q5RKI1, RN |
Family and domain databases
CDDi | cd18046, DEADc_EIF4AII_EIF4AI_DDX2, 1 hit |
Gene3Di | 3.40.50.300, 2 hits |
InterProi | View protein in InterPro IPR011545, DEAD/DEAH_box_helicase_dom IPR044728, EIF4A_DEADc IPR014001, Helicase_ATP-bd IPR001650, Helicase_C IPR027417, P-loop_NTPase IPR000629, RNA-helicase_DEAD-box_CS IPR014014, RNA_helicase_DEAD_Q_motif |
Pfami | View protein in Pfam PF00270, DEAD, 1 hit PF00271, Helicase_C, 1 hit |
SMARTi | View protein in SMART SM00487, DEXDc, 1 hit SM00490, HELICc, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
PROSITEi | View protein in PROSITE PS00039, DEAD_ATP_HELICASE, 1 hit PS51192, HELICASE_ATP_BIND_1, 1 hit PS51194, HELICASE_CTER, 1 hit PS51195, Q_MOTIF, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | IF4A2_RAT | |
Accessioni | Q5RKI1Primary (citable) accession number: Q5RKI1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2006 |
Last sequence update: | December 21, 2004 | |
Last modified: | February 23, 2022 | |
This is version 133 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- Translation initiation factors
List of translation initiation factor entries - SIMILARITY comments
Index of protein domains and families