UniProtKB - Q5RJX8 (KT5BB_XENLA)
Protein
Histone-lysine N-methyltransferase KMT5B-B
Gene
kmt5b-b
Organism
Xenopus laevis (African clawed frog)
Status
Functioni
Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (By similarity). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Plays a role in myogenesis by regulating the expression of target genes, such as EID3. Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).By similarity
Catalytic activityi
- N6-methyl-L-lysyl20-[histone H4] + S-adenosyl-L-methionine = H+ + N6,N6-dimethyl-L-lysyl20-[histone H4] + S-adenosyl-L-homocysteineBy similarityEC:2.1.1.362By similarityThis reaction proceeds in the forwardBy similarity direction.
- N6,N6-dimethyl-L-lysyl20-[histone H4] + S-adenosyl-L-methionine = H+ + N6,N6,N6-trimethyl-L-lysyl20-[histone H4] + S-adenosyl-L-homocysteineBy similarityThis reaction proceeds in the forwardBy similarity direction.
- L-lysyl20-[histone H4] + S-adenosyl-L-methionine = H+ + N6-methyl-L-lysyl20-[histone H4] + S-adenosyl-L-homocysteineBy similarityEC:2.1.1.361By similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 27 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 116 | S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity | 1 | |
Binding sitei | 163 | S-adenosyl-L-methionineBy similarity | 1 | |
Metal bindingi | 181 | ZincBy similarity | 1 | |
Metal bindingi | 225 | ZincBy similarity | 1 | |
Binding sitei | 226 | S-adenosyl-L-methionine; via amide nitrogenBy similarity | 1 | |
Metal bindingi | 227 | ZincBy similarity | 1 | |
Metal bindingi | 230 | ZincBy similarity | 1 |
GO - Molecular functioni
- chromatin binding Source: UniProtKB
- histone methyltransferase activity (H4-K20 specific) Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- S-adenosyl-L-methionine binding Source: UniProtKB
GO - Biological processi
- DNA repair Source: UniProtKB
- histone H4-K20 dimethylation Source: InterPro
- muscle organ development Source: UniProtKB-KW
- positive regulation of double-strand break repair via nonhomologous end joining Source: UniProtKB
- positive regulation of isotype switching Source: UniProtKB
Keywordsi
Molecular function | Chromatin regulator, Methyltransferase, Repressor, Transferase |
Biological process | Myogenesis, Transcription, Transcription regulation |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase KMT5B-BCuratedAlternative name(s): Lysine-specific methyltransferase 5B-BBy similarity Suppressor of variegation 4-20 homolog 1-B Short name: Su(var)4-20 homolog 1-B Short name: Suv4-20h1-B [histone H4]-N-methyl-L-lysine20 N-methyltransferase KMT5BCurated (EC:2.1.1.362By similarity) [histone H4]-lysine20 N-methyltransferase KMT5BCurated (EC:2.1.1.361By similarity) |
Gene namesi | Name:kmt5b-bBy similarity Synonyms:suv420h1-b |
Organismi | Xenopus laevis (African clawed frog) |
Taxonomic identifieri | 8355 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Xenopus |
Organism-specific databases
Xenbasei | XB-GENE-6253211, kmt5b.L |
Subcellular locationi
Nucleus
Other locations
- Chromosome By similarity
Note: Associated with pericentric heterochromatin.By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- chromosome Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000281792 | 1 – 785 | Histone-lysine N-methyltransferase KMT5B-BAdd BLAST | 785 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 458 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Ubl conjugationFamily & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 99 – 214 | SETPROSITE-ProRule annotationAdd BLAST | 116 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 109 – 112 | S-adenosyl-L-methionine bindingBy similarity | 4 | |
Regioni | 178 – 179 | S-adenosyl-L-methionine bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.PROSITE-ProRule annotation
Phylogenomic databases
OMAi | TACESIP |
Family and domain databases
CDDi | cd19184, SET_KMT5B, 1 hit |
Gene3Di | 1.10.10.1700, 1 hit |
InterProi | View protein in InterPro IPR041938, Hist-Lys_N-MTase_N IPR044424, KMT5B_SET IPR001214, SET_dom IPR039977, Suv4-20/Set9 IPR025790, Suv4-20_animal |
PANTHERi | PTHR12977, PTHR12977, 1 hit |
Pfami | View protein in Pfam PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM00317, SET, 1 hit |
PROSITEi | View protein in PROSITE PS51570, SAM_MT43_SUVAR420_2, 1 hit PS50280, SET, 1 hit |
i Sequence
Sequence statusi: Complete.
Q5RJX8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSAKELCEND DLATSLVLDP YLGFQTHKMN TRFRPIKGRQ EELKEVIENF
60 70 80 90 100
KKNEQLEKTF KNLVAGDWAR HYFLHKNKMQ ETHFKAHVFI YLRMFATSSG
110 120 130 140 150
FEILPCCRYS SERNGAKIVA TKDWKRNDKI ELLVGCIAEL SEAEENMLLR
160 170 180 190 200
HGENDFSVMY STRKNCAQLW LGPAAFINHD CRPNCKFVST GRDTACVKAL
210 220 230 240 250
RDIEPGEEIS CYYGDGFFGE NNEFCECYTC ERRATGAFKS RVGLNEPGPV
260 270 280 290 300
INSKYGLRET DKRLNRLKKL GDNGKNSDSQ SVSSNTDADT SQEKNTANRK
310 320 330 340 350
SNGLRKKSKS RTLRRQSMSR IPISSSSTSS KLPHINNSRV PKRLRKTAKP
360 370 380 390 400
LHSKLKIRCH RKKVEQKKTS KKLDVSNLVL KEPKVVLYKN LAIKKDRESQ
410 420 430 440 450
GAVQVTETTG CLTRHAAREY KLNSFKGAYA HGDTSPCTYI TRSSLRTRFN
460 470 480 490 500
SKDMSEAKLQ PNSVDGYRSS HGTVIQLDTG DPLFQSTRKN ELLQETSRQS
510 520 530 540 550
MRFQRNNFNT SRRNSRQNRY ITQASKVEDS VSIYNSSSID NSLPDLGNSH
560 570 580 590 600
CDLGEGNALI HTSPDYKNIK SSTDEYPLVT SEITKSKKNI RTVKNKKRRR
610 620 630 640 650
ITRYDAQLIL ENSTGIPKLT LRRRHDSNSS KTNEKENEGM GSSKISIKLS
660 670 680 690 700
KDHEKDKNSL YVAKLNNGFN SGSGSTSTKL KIQLKRDEEN RMAFPNENGM
710 720 730 740 750
YCSDTLSLLG TRMEVDGYDH YEEESVGESS TEEEEEEEDE FDDEFEDDFI
760 770 780
PLPPAKRLRL IVGKDSIDID ISSRRREDQS LRLNA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC086459 mRNA Translation: AAH86459.1 |
RefSeqi | NP_001088653.1, NM_001095184.1 |
Genome annotation databases
GeneIDi | 495826 |
KEGGi | xla:495826 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC086459 mRNA Translation: AAH86459.1 |
RefSeqi | NP_001088653.1, NM_001095184.1 |
3D structure databases
SMRi | Q5RJX8 |
ModBasei | Search... |
Genome annotation databases
GeneIDi | 495826 |
KEGGi | xla:495826 |
Organism-specific databases
CTDi | 495826 |
Xenbasei | XB-GENE-6253211, kmt5b.L |
Phylogenomic databases
OMAi | TACESIP |
Family and domain databases
CDDi | cd19184, SET_KMT5B, 1 hit |
Gene3Di | 1.10.10.1700, 1 hit |
InterProi | View protein in InterPro IPR041938, Hist-Lys_N-MTase_N IPR044424, KMT5B_SET IPR001214, SET_dom IPR039977, Suv4-20/Set9 IPR025790, Suv4-20_animal |
PANTHERi | PTHR12977, PTHR12977, 1 hit |
Pfami | View protein in Pfam PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM00317, SET, 1 hit |
PROSITEi | View protein in PROSITE PS51570, SAM_MT43_SUVAR420_2, 1 hit PS50280, SET, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KT5BB_XENLA | |
Accessioni | Q5RJX8Primary (citable) accession number: Q5RJX8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 3, 2007 |
Last sequence update: | December 21, 2004 | |
Last modified: | April 7, 2021 | |
This is version 68 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |