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UniProtKB - Q5RJP0 (ALD1_RAT)
Protein
Aldo-keto reductase family 1 member B7
Gene
Akr1b7
Organism
Rattus norvegicus (Rat)
Status
Functioni
Reduces a broad range of aliphatic and aromatic aldehydes to the corresponding alcohols (PubMed:21048316).
Reduces prostaglandins (By similarity).
May play a role in the metabolism of xenobiotic aromatic aldehydes.
By similarity1 PublicationCatalytic activityi
- EC:1.1.1.212 Publications
Activity regulationi
Inhibited by tolrestat and epalrestat.1 Publication
Kineticsi
- KM=1.5 µM for NADPH2 Publications
- KM=220 µM for NADH2 Publications
- KM=0.16 µM for 4-oxo-2-nonenal2 Publications
- KM=37 µM for geraniol2 Publications
- KM=1.5 µM for 4-nitrobenzaldehyde2 Publications
pH dependencei
Optimum pH is 6.5-7.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 44 | NADP1 Publication | 1 | |
Active sitei | 49 | Proton donorBy similarity | 1 | |
Sitei | 78 | Lowers pKa of active site TyrBy similarity | 1 | |
Binding sitei | 184 | NADP1 Publication | 1 | |
Binding sitei | 273 | NADP1 Publication | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 20 – 21 | NADP1 Publication | 2 | |
Nucleotide bindingi | 160 – 161 | NADP1 Publication | 2 | |
Nucleotide bindingi | 210 – 215 | NADP1 Publication | 6 | |
Nucleotide bindingi | 263 – 269 | NADP1 Publication | 7 |
GO - Molecular functioni
- alditol:NADP+ 1-oxidoreductase activity Source: GO_Central
- aldo-keto reductase (NADP) activity Source: RGD
- prostaglandin H2 endoperoxidase reductase activity Source: RHEA
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
Reactomei | R-RNO-193144, Estrogen biosynthesis R-RNO-975634, Retinoid metabolism and transport |
Names & Taxonomyi
Protein namesi | Recommended name: Aldo-keto reductase family 1 member B7 (EC:1.1.1.212 Publications)Alternative name(s): Aldehyde reductase Aldose reductase-like protein AKR1B141 Publication Aldose reductase-related protein 1 |
Gene namesi | Name:Akr1b7 Synonyms:Akr1b14 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620257, Akr1b7 |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Cytosol
- cytosol Source: GO_Central
Mitochondrion
- mitochondrion Source: GO_Central
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 269 | H → F or R: Reduced affinity for NADP. 1 Publication | 1 | |
Mutagenesisi | 269 | H → M: Strongly reduced affinity for NADP. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL3421523 |
DrugCentrali | Q5RJP0 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000415351 | 1 – 316 | Aldo-keto reductase family 1 member B7Add BLAST | 316 |
Proteomic databases
jPOSTi | Q5RJP0 |
PaxDbi | Q5RJP0 |
PRIDEi | Q5RJP0 |
PTM databases
iPTMneti | Q5RJP0 |
PhosphoSitePlusi | Q5RJP0 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000009875, Expressed in female gonad and 15 other tissues |
Genevisiblei | Q5RJP0, RN |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000013423 |
Chemistry databases
BindingDBi | Q5RJP0 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | Q5RJP0 |
SMRi | Q5RJP0 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q5RJP0 |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldo/keto reductase family.Curated
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000154773 |
HOGENOMi | CLU_023205_0_0_1 |
InParanoidi | Q5RJP0 |
OMAi | NTFHEKS |
OrthoDBi | 1016440at2759 |
PhylomeDBi | Q5RJP0 |
TreeFami | TF106492 |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR018170, Aldo/ket_reductase_CS IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q5RJP0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTFVKLRTK AKMPLVGLGT WKSPPGQVKE AVKAAIDAGY RHFDCAYVYQ
60 70 80 90 100
NESEVGEAIQ EKIKEKAVRR EDLFIVSKLW STFFEKSLMK EAFQKTLSDL
110 120 130 140 150
KLDYLDLYLI HWPQGLQAGK EFLPKDSQGK VLMSKSTFLD AWEGMEELVD
160 170 180 190 200
QGLVKALGVS NFNHFQIERL LNKPGLKHKP VTNQVECHPY LTQEKLIQYC
210 220 230 240 250
HSKGIAVIAY SPLGSPDRPY AKPEDPVVLE IPKIKEIAAK HKKTIAQVLI
260 270 280 290 300
RFHVQRNVAV IPKSVTLSHI KENIQVFDFQ LSEEDMAAIL SLNRNWRACG
310
LFVTSDEEDF PFHEEY
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF7EMK8 | F7EMK8_RAT | Aldo-keto reductase family 1 member... | Akr1b7 | 315 | Annotation score: | ||
A0A8I6A3C9 | A0A8I6A3C9_RAT | Aldo-keto reductase family 1 member... | Akr1b7 | 303 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH473959 Genomic DNA Translation: EDM15308.1 BC086563 mRNA Translation: AAH86563.1 |
RefSeqi | NP_446233.2, NM_053781.2 |
Genome annotation databases
Ensembli | ENSRNOT00000013423; ENSRNOP00000013423; ENSRNOG00000009875 |
GeneIDi | 116463 |
KEGGi | rno:116463 |
UCSCi | RGD:620257, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | CH473959 Genomic DNA Translation: EDM15308.1 BC086563 mRNA Translation: AAH86563.1 |
RefSeqi | NP_446233.2, NM_053781.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3O3R | X-ray | 1.86 | A/B | 1-316 | [»] | |
3QKZ | X-ray | 1.87 | A/B | 1-316 | [»] | |
AlphaFoldDBi | Q5RJP0 | |||||
SMRi | Q5RJP0 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000013423 |
Chemistry databases
BindingDBi | Q5RJP0 |
ChEMBLi | CHEMBL3421523 |
DrugCentrali | Q5RJP0 |
PTM databases
iPTMneti | Q5RJP0 |
PhosphoSitePlusi | Q5RJP0 |
Proteomic databases
jPOSTi | Q5RJP0 |
PaxDbi | Q5RJP0 |
PRIDEi | Q5RJP0 |
Genome annotation databases
Ensembli | ENSRNOT00000013423; ENSRNOP00000013423; ENSRNOG00000009875 |
GeneIDi | 116463 |
KEGGi | rno:116463 |
UCSCi | RGD:620257, rat |
Organism-specific databases
CTDi | 11997 |
RGDi | 620257, Akr1b7 |
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000154773 |
HOGENOMi | CLU_023205_0_0_1 |
InParanoidi | Q5RJP0 |
OMAi | NTFHEKS |
OrthoDBi | 1016440at2759 |
PhylomeDBi | Q5RJP0 |
TreeFami | TF106492 |
Enzyme and pathway databases
Reactomei | R-RNO-193144, Estrogen biosynthesis R-RNO-975634, Retinoid metabolism and transport |
Miscellaneous databases
EvolutionaryTracei | Q5RJP0 |
PROi | PR:Q5RJP0 |
Gene expression databases
Bgeei | ENSRNOG00000009875, Expressed in female gonad and 15 other tissues |
Genevisiblei | Q5RJP0, RN |
Family and domain databases
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR020471, AKR IPR018170, Aldo/ket_reductase_CS IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ALD1_RAT | |
Accessioni | Q5RJP0Primary (citable) accession number: Q5RJP0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 25, 2012 |
Last sequence update: | December 21, 2004 | |
Last modified: | May 25, 2022 | |
This is version 115 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families